ABSTRACT
Platinum nanoparticles (Pt NPs) with properties including damage of DNA, enzmatic activity and possibility of light absorption in the biological range could find application as effective photosensitizers in photothermal anticancer therapy (PTT). The photothermal properties of Pt NPs depend on their shape, size and crystalline structure. Therefore, in this paper the effect of Pt NPs size on photothermal efficiency is determined. For this purpose, spherical, crystalline 80â¯nm PtI NPs and 2â¯nm PtII NPs were synthesized and characterized by transmission electron microscopy (TEM), selected area diffraction patterns (SAED) and X-ray diffraction (XRD). The possibilities of using Pt NPs in PTT were investigated using two colon cancer cell lines: SW480 and SW620, which were cultured with both Pt NPs and irradiated by two, 650â¯nm and 808â¯nm lasers. Microscopy images of cells and MTS assay showed, that the PTT is the most effective when 2â¯nm nanoparticles and the 650â¯nm laser were used. The mortality of cells was around 62% for SW480 and 70% for SW620. Furthermore, higher temperatures after irradiation of Pt NPs by lasers were observed for the 2â¯nm Pt NPs for both wavelengths. Consequently, the values of photothermal efficiency are higher by approximately 5% and 6% for 2â¯nm Pt NPs, than for 80â¯nm ones, which were irradiated by 650 and 808â¯nm lasers, respectively. Moreover, the results obtained from experimental data corresponded with Mie theory.
Subject(s)
Metal Nanoparticles , Nanoparticles , Photochemotherapy , Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Photothermal Therapy , PlatinumABSTRACT
In this work, we propose a new method for the large-scale production of flexible, periodic alumina arrays with well-ordered pores. We show the incorporation of pre-patterning based on polystyrene (PS) nanosphere lithography into an aluminium anodization process. We prepared ordered monolayers of PS spheres with average diameters of (510 ± 10) nm and (430 ± 10) nm on a large area (1.5 × 1.5 cm2) of the Si substrate. Next, we deposited a 5 µm aluminium layer on arrays of PS nanospheres using the sputtering technique. After the deposition, we covered the aluminium film with a polymer Scotch adhesive tape, and separated it from the silicon substrate by ultrasonic-assisted lift-off. Finally, we performed the anodization of the aluminium. We compared the pore and cell sizes, and the pore distance for the templates obtained by this technique, with reference to the templates prepared by a two-step anodization process. Using this new approach, we obtained highly ordered hexagonal 2D lattices over a large area of up to 2 cm2 with sparse defects, amounting to not more than four defects per 1000 µm2 on average. Here, we show that the use of indentation techniques is not necessary and can be replaced by a fast, cheap and easy pre-patterning step based on nanosphere lithography.