ABSTRACT
Chemical modifications of guanine are some of the most common results of interactions of DNA with many carcinogens and anti-cancer drugs, including nitrosoureas, nitrogen mustards, triazenes, polycyclic aromatics, and aflatoxins. The base sequence specificity for alkylation of guanines by three 2-chloroethylnitrosoureas has been determined. Guanines in the midst of a run of guanines are more susceptible than guanines in other base sequences. We have shown that certain 2-chloroethylnitrosoureas (BCNU, CCNU and methyl-CCNU) follow this same pattern. However, the quantitative degree of higher specificity for guanine with guanines as nearest neighbors depended on both the guanine position alkylated and the structure of the alkyl group attached. For example, when hydroxyethylation of runs of guanine occurred at N-7, a 6- to 11-fold increase of alkylation occurred compared to that found in the random base sequences of DNA, while hydroxyethylation at O-6 increased 1.2 to 3.5-fold and chloroethylation at N-7 was 2-to 4-fold higher than in DNA. Guanines with thymines on both the 3' and 5' sides were much less susceptible, most notably in N-7-hydroxyethylation and N-7-chloroethylation. Since guanine-rich regions are found in regulatory regions of the genome, knowledge concerning the effect of base sequence upon the production of each of the potential DNA lesions is vital to gaining an understanding of the roles of these lesions in the anti-tumor activity of a drug.
Subject(s)
Carmustine/pharmacology , DNA/drug effects , Guanine , Lomustine/pharmacology , Nitrosourea Compounds/pharmacology , Semustine/pharmacology , Alkylation , Base Sequence , Chemical Phenomena , Chemistry , Chromatography, High Pressure Liquid , Structure-Activity RelationshipABSTRACT
The Saturation Kinetics Model (SKM) is useful in describing many physiological responses as functions of a limiting dietary nutrient. However, as nutrients are fed at higher dietary concentrations, responses become inhibited and diminish from their usual plateaus. By adding an inhibition constant (Ks) to the SKM in a manner consistent with substrate inhibition (based on enzyme kinetics), it becomes possible to predict the inhibited portions of the nutrient-response curve. To test this, rats were fed diets of graded levels of casein (0-75%) or lysine (0-6.2%), and weight gains and food intakes were measured daily for up to 2 wk. The inhibition form of the SKM was able to predict the complete response range of each experiment, producing a Ks (weight gain) at a dietary level of 50.60% for casein and 7.56% for lysine. It was also possible to set up an upper and lower dietary nutrient concentration that encompassed the 100% response range for each response, thereby giving an inhibition or toxicity index of 2.02 for casein and 4.98 for lysine. This index allows one to set nutritional requirement levels precisely, optimizing responses without moving into inhibiting or toxic ranges of nutrients. Based on growth response curves, requirements were 25.61% for casein and 1.97% for lysine.
Subject(s)
Mathematics , Models, Biological , Nutritional Requirements , Animals , Body Weight/drug effects , Caseins/deficiency , Caseins/pharmacology , Caseins/toxicity , Diet , Energy Intake/drug effects , Kinetics , Lysine/deficiency , Lysine/pharmacology , Lysine/toxicity , RatsABSTRACT
Urinary metabolites of ring 14C-labeled 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) and 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea (Methyl CCNU) from rats have been isolated and characterized by high-performance liquid chromatography and mass spectrometry. About 44% of the cyclohexyl moiety of CCNU was excreted in 24 hr and included approximately 10% of the excreted dose as free amines and 40% as conjugates that could be converted to amines by hydrolysis. Amine composition of free base plus hydrolyzable conjugates was 55% hydroxycyclohexylamines (3-trans, 3-cis, 4-cis, and 4-trans) and 30% cyclohexylamine. This strongly supports previous studies which indicated that CCNU is largely hydroxylated in vivo as well as in vitro. Rats pretreated with phenobarbital excreted high relative amounts of cis-4-hydroxy derivatives (41%), again showing a high degree of correlation between in vitro and in vivo results. Treatment of urine with beta-glucuronidase gave no apparent increase in free amines. However, sulfatase was about 25% as effective as alkaline hydrolysis for releasing free amines from whole urine. Major urinary metabolites were found to have m.w. of about 629, 413, 329, and 243 and represented 55%, 20%, 20%, and 5% of total excreted 14C, respectively. It was concluded that the higher m.w. metabolites may be conjugates of peptides possibly derived from active site-directed inactivation of specific enzymes. Previous work has shown that enzymes such as chymotrypsin and glutathione reductase are inhibited by isocyanates in this manner. Hydroxylated metabolites of Methyl CCNU had a pattern similar to that of CCNU. The major free (12%) and conjugated amine (54%) metabolites of Methyl CCNU in the urine in decreasing order of quantity present were cis-3-hydroxy-trans-4-methylcyclohexylamine, trans-4-methylcyclohexylamine, trans-4-hydroxymethylcyclohexylamine, and trans-3-hydroxy-trans-4-methyl-cyclohexylamine.
Subject(s)
Lomustine/urine , Nitrosourea Compounds/urine , Semustine/urine , Amines/urine , Animals , Biotransformation , Cyclohexylamines/urine , Hydrolysis , Male , Peptides/metabolism , RatsABSTRACT
Liver microsomal metabolism of 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea in the presence of reduced nicotinamide adenine dinucleotide phosphate and O2 was shown to produce seven metabolites that included the parent urea. A cytochrome P-450-dependent monohydroxylation of the cyclohexyl ring occurred in 3 positions, cis-3, trans-3, and cis-4, and on the methyl group to form a trans-4-hydroxymethyl derivative. In addition, monohydroxylation of the 2-chloroethyl carbon attached to the N-1 urea nitrogen yielded an alpha-hydroxy metabolite. A ring-hydroxylated derivative remained unidentified while the structures of all other such derivatives were established by comparison with compound synthesized, purified by high-pressure liquid chromatography, and characterized by mass spectral and nuclear magnetic resonance analyses. It was tentatively concluded that some parent urea is formed by a cytochrome P-450 dependent reaction because of a requirement for reduced nicotinamide adenine dinucleotide phosphate and inhibition by CO. Microsomes from rats pretreated with phenobarbital showed about a 3-fold increase in hydroxylation rate while phenobarbital-treated mice microsomes were induced 8-fold. However, in both species, the induced hydroxylation rate was about 4 nmol/min/mg protein. When microsomes from phenobarbital-induced rats were used, a mixture of 80% CO:20% O2 decreased the rate of formation of all metabolites to 14% of that in 80% N2:20% O2.
Subject(s)
Microsomes, Liver/metabolism , Nitrosourea Compounds/metabolism , Semustine/metabolism , Animals , Carbon Monoxide/pharmacology , Chemical Phenomena , Chemistry , Hydroxylation , In Vitro Techniques , Magnetic Resonance Spectroscopy , Male , Mass Spectrometry , Mice , Phenobarbital/pharmacology , Rats , Semustine/analogs & derivatives , Semustine/chemical synthesisABSTRACT
Liver microsomal hydroxylation of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea was shown to occur on the cyclohexyl ring at positions 3 and 4. Four metabolites were isolated by selective solvent extraction and purifed by high-pressure liquid chromatography. cis-4-, trans-4-, cis-3-, and trans-3-OH derivatives of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea were synthesized and their chromatographic, mass spectral, and nuclear magnetic resonance characteristics matched those of the metabolites. The position of ring hydroxylation and the identity of each geometric isomer were established by nuclear magnetic resonance using a shift reagent in conjunction with spin decoupling techniques. Microsomes from rats pretreated with phenobarbital showed a sixfold increase in hydroxylation rate (19.5 vs. 3.3 nmol per mg per min). The induction was quite selective for cis-4 hydroxylation (19-fold); however, induction of trans-4 (threefold), cis-3 (threefold), and trans-3 (twofold) hydroxylation did occur. Quantitatively the cis-4-hydroxy metabolite was 67of the total product by phenobarbital-induced microsomes and 21% for normal microsomes. Microsomes from animals pretreated wit- 3-methyl-cholanthrene gave about the same rate and product distribution that normal microsomes gave. A mixture of 80% carbon monoxide-20% oxygen inhibited formation of all four hydroxy metabolites with the inhibition ranging from 55 to 78%.
Subject(s)
Antineoplastic Agents/metabolism , Lomustine/metabolism , Microsomes, Liver/metabolism , Nitrosourea Compounds/metabolism , Animals , Carbon Monoxide/pharmacology , Hydroxylation , Male , Methylcholanthrene/pharmacology , Microsomes, Liver/drug effects , Nitrosourea Compounds/chemical synthesis , Oxidation-Reduction , Phenobarbital/pharmacology , Rats , StereoisomerismSubject(s)
Lomustine , Nitrosourea Compounds , Acetates/biosynthesis , Acetates/urine , Alkylation , Animals , Carbamates , Carbocysteine/urine , Dicarboxylic Acids/biosynthesis , Dicarboxylic Acids/urine , Ethane/analogs & derivatives , Ethane/metabolism , Ethylene Chlorohydrin/metabolism , Halogens , Hydroxylation , Isomerism , Lomustine/analogs & derivatives , Lomustine/metabolism , Male , Methylcholanthrene/pharmacology , Microsomes , Phenobarbital/pharmacology , Rats , Sulfides/biosynthesis , Sulfides/urineABSTRACT
Chemical degradation of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea or 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea in buffer under physiological conditions resulted in the formation of a significant quantity of 2-chlorethanol (18 to 25% of the initial nitrosourea concentration). Other degradation products observed included acetaldehyde (5 to 10%), vinyl chloride (1 to 2%), ethylene (1 to 2%), and cyclohexylamine (32%), but not 1,3-dicyclohexylurea. The 2-chlorethyl moiety of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea was trapped with halide ions, CI-, BR-, and I-, to form the corresponding dihaloethanes which were identified by gas chromatography-mass spectrometry techniques. High-pressure liquid chromatographic procedures were developed for the separation and quantiation of the nitrosoureas and many of their degradation products. It is postulated that a new mode of 1(2-chloreoethyl)-3-cyclohexyl-1-nitrosourea and 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea degradation can occur that is not the loss of the chloro group as chloride ion, but the loss of the N-3 hydrogen as a proton. Then the corresponding isocyanate and 2-chloroethyidiazene hydroxide are formed, with the latter intermidiate becoming an alkylating species, possibly in part as a 2-chloroethyl carbonium ion.