ABSTRACT
BACKGROUND: Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity. OBJECTIVE: To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR. METHODS: BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs). RESULTS: The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs. CONCLUSION: This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.
Subject(s)
Calcification, Physiologic , Pericardium , Cattle , Animals , Glutaral/analysis , Glutaral/pharmacology , Cell Adhesion , Tissue Scaffolds/chemistryABSTRACT
BACKGROUND: Oral lichen planus is a chronic inflammatory skin disease of autoimmune nature, with infiltration of T lymphocytes that destroy the basal stratum, which causes white striae, erosions, ulcers and breaks in epithelial continuity. CASE REPORT: 54-year-old woman with multiple reticular and erosive lesions in the region of the buccal mucosa and lateral borders of the tongue, as well as desquamative gingivitis. Treatment with mometasone was started thrice daily for 20 days and intermittently with miconazole gel to prevent iatrogenic mycosis by the corticosteroid. The patient was examined at 20 days, after which 80 % improvement of the lesions that had central ulcerations was observed, which allowed the ingestion of food and fluids without pain and improved the quality of life. CONCLUSIONS: In autoimmune diseases, clinical-histopathological correlation is important for a definitive diagnosis to be established. Clinical follow-up of these patients should be carried out in the long term, in order to achieve remission of the lesions, control of the disease and avoid future complications.
Antecedentes: El liquen plano bucal es una enfermedad dermatológica inflamatoria crónica de base autoinmune, con infiltración de linfocitos T que destruyen el estrato basal, lo que ocasiona estrías blancas, erosiones, úlceras y diluciones epiteliales de continuidad. Reporte de caso: Mujer de 54 años con múltiples lesiones reticulares y erosivas en región de la mucosa yugal y bordes laterales de la lengua, así como gingivitis descamativa. Se inició tratamiento con mometasona tres veces al día por 20 días e intermitentemente con miconazol en gel, para prevenir micosis iatrogénica por el corticosteroide. La paciente fue revisada a los 20 días, al cabo de los cuales se observó mejoría de 80 % de las lesiones con ulceraciones centrales, lo que permitió la ingesta de alimentos y líquidos sin dolor y mejoró la calidad de vida. Conclusiones: En las enfermedades autoinmunes es importante la correlación clínico-histopatológica para establecer el diagnóstico definitivo. El seguimiento clínico de estos pacientes se debe realizar a largo plazo, para lograr la remisión de las lesiones, control de la enfermedad y evitar futuras complicaciones.
Subject(s)
Lichen Planus, Oral , Female , Humans , Lichen Planus, Oral/diagnosis , Lichen Planus, Oral/drug therapy , Middle AgedABSTRACT
El virus del papiloma humano (VPH) forma parte de un grupo de virus ADN heterogéneo llamados papillomaviridae; este virus es causante de múltiples lesiones hiperplásicas, verrucosas y papilomatosas de las células epiteliales de piel y mucosas, existen más de 120 tipos de VPH, de solo 100 se conoce su secuencia genómica completa. Los dos géneros más importantes son los papilomavirus alpha (ð) y los beta (ß), la mayoría de los virus que infectan el área genital pertenecen al género alpha; según sus características clínicas, se pueden subdividir en cutáneos y de mucosa. Si bien las infecciones por este virus son frecuentes en cavidad oral, este campo de la medicina se encuentra en estudio, debido a que la información sobre el tema no es concluyente, es prioritario para el estomatólogo conocer las generalidades acerca del VPH y tratar las lesiones de una manera informada, este virus se asocia a un 35% de los casos de cáncer de cavidad oral, de los cuales el 70% de ellos son de alto riesgo. En México, se tiene estimado que cerca del 43% de los hombres y del 17,5% de las mujeres, todos ellos sanos y sexualmente activos tienen alguna infección por VPH.
The human papillomavirus (HPV) is part of heterogeneous group of DNA viruses called papillomaviridae; this virus causes multiple hyperplastic, papillomatous and warty lesions of epithelial cells of skin and mucosa, there are more than 120 types of HPV, although, the complete genome sequence of only 100 of these is known. The most important types are the alpha papillomavirus (a) and beta (b), most viruses that infect the genital area belong to the alpha type; according to their clinical characteristics, they can be divided in cutaneous and mucosal. HPV infections are common in oral cavity, this field of medicine is currently being studied, considering that the information about this subject is not conclusive, it is a priority for the stomatologist to know the generalities about HPV and treat injuries in an informed manner; this virus is associated to a 35% of cases of oral cancer, of which 70% are considered high risk. In Mexico, it is estimated that about a 43% of men and a 17.5% of women, all of them healthy and sexually active have at least one HPV infection.
