ABSTRACT
The techniques of assisted reproduction have recently become the most effective methods of treatment of infertility; namely ICSI (intra-cytoplasmic sperm injection), MESA (microepididymal sperm aspiration), TESA (testicular sperm aspiration) and TESE (testicular sperm extraction). The techniques have been increasingly successful, even above the average efficiency of classical IVF (in vitro fertilization). It can be demonstrated by the percentage of ICSI-aided births percentage per 100% of embryotransfers in ISCARE; 1996 = 21.3%, 1997 = 26.15%, 1998 = 29.7%. The successful use of these techniques is associated with the rise of risks which result from the selection of couples for assisted reproduction with genetic-based infertility and with the rise of risks involving the introduction of genetic-based defects in to the next generation. Presently, a list of indications is being developed, which, while still not accepted officially, identifies patients for genetic counselling. Only the counselling center has the competence to estimate genetic risks over generations. Subsequently, after selection by the center, during 1997 and 1998 the chromosomes of 731 patients were cytogenetically examined, representing 429 infertile couples from the centres ISCARE, PRONATAL, FERTIMED, and CAR 1. LF UK. Within these 429 couples, belonging to four groups of indications, the cytogenetic examination was informative in 8.15%. This finding of a relatively high percentage (10 times more than in the general population) confirms the validity of the list of indications and the necessity of cooperation among the genetic counselling center, cytogenetic laboratory and the IVF centre.
Subject(s)
Cytogenetic Analysis , Genetic Counseling , Infertility/therapy , Reproductive Techniques , Chromosome Aberrations/diagnosis , Chromosome Disorders , Female , Fertilization in Vitro , Humans , Interprofessional Relations , MaleABSTRACT
A Real Time PCR system (Q-PCR) for the detection and quantification ofAspergillus flavus in black pepper has been used. Thenor1 gene, a gene of the aflatoxin biosynthetic pathway, served as target sequence. The determined copy numbers of thenor1 gene were compared to the cfu numbers obtained by plate counting. There was not a direct correlation of the results with both approaches, however the tendency was the same. In general both values increased with prolonged incubation time. The differences in copy numbers of thenor1 gene to cfu numbers decreased with increasing incubation time.
Subject(s)
Cryopreservation , Embryo, Mammalian , Reproductive Techniques , Fertilization in Vitro , HumansABSTRACT
During the period between February 1996 and August 1997 51 infertile men were operated. The authors evaluate the results from 47 couples--25 cases of testicular azoospermia and 22 cases of obstructive azoospermia. The mean age of the men in the group was 33 years (22-48 years). The follow-up period is 1-18 months. In 17 cases, microsurgical epididymal sperm aspiration (MESA) was made, in five cases testicular sperm aspiration (TESA) and in 25 men, testicular sperm extraction (TESE) was performed. In the group with testicular azoospermia it proved possible to obtain sperm in 12 of 25 cases, i.e. in 48%. In men with obstructive azoospermia all aspirations were successful, i.e. the yield was 100%. In this group five children were born, seven pregnancies are under way. Thus regardless of the etiology of male infertility 12 of 47 cases, i.e. 25.5% were resolved successfully. When the results of the two groups are differentiated, the outcome is as follows: in the group with testicular azoospermia one child was born and two pregnancies are under way, i.e. 12%, in the group with obstructive azoospermia four children were born and five pregnancies are under way, i.e. 40.9%.
Subject(s)
Infertility, Male/therapy , Reproductive Techniques , Adult , Female , Humans , Infertility, Male/etiology , Male , Microsurgery , Middle Aged , PregnancySubject(s)
Infertility, Male/therapy , Reproductive Techniques , Spermatocele/complications , Adult , Humans , Infertility, Male/etiology , MaleABSTRACT
A one-year study of 51 infertile couples, 47 couples evaluated--25 cases of testicular azoospermia and 22 cases of obstructive azoospermia. The mean age of the men in the group is 33 years (22-48 years). The follow-up period is 1-18 months. In 17 instances microsurgical epididymal sperm aspiration--MESA was made, five times testicular sperm aspiration--TESA and in 25 men testicular sperm extraction--TESE was used. In the group with testicular azoospermia it proved possible to obtain sperm in 12/25 cases, i.e. in 48%. In men with obstructive azoospermia all aspirations were successful, i.e. the yield was 100%. In this group five infants were born, another seven pregnancies are under way. Thus regardless of the etiology of male infertility 12/47 cases, i.e. 25.5%, were successfully resolved. When using differentiated evaluation of the two groups the results are as follows: in the group with testicular azoospermia one infant were born and five pregnancies are under way, i.e. 40.9%.
