GO/AgNW aided sustained release of ciprofloxacin loaded in Starch/PVA nanocomposite mats for wound dressings application.

Compared to conventional bandages, which do not meet all wound care requirements, nanofiber wound dressings could provide a potentially excellent environment for healing. In the present research, nanocomposite membrane based on starch (St) - polyvinyl alcohol (PVA) nanofibers containing ciprofloxacin antibiotic drug loaded on graphene oxide­silver nanowire (GO-AgNWs) hybrid nanoparticles is produced by electrospinning process. Morphological studies showed that the length and diameter of silver nanowires are 21 ± 9.17 µm and 82 ± 10.52 nm, respectively. The contact angle of 57.1° due to the hydrophilic nature of nanofibers, also the swelling degree of 679.51 % and, the water vapor permeability of 2627 ± 56 (g/m2.day) can be expressed as a confirmation of the ability of this wound dressing to manage secretions around the wound. In evaluating the antibacterial activity of these nanocomposite membranes against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, the most potent antibacterial effect is in the case of nanofibers containing a high percentage of starch and nanoparticles carrying ciprofloxacin; with non-growth halos of 47.58 mm and 22.06 mm was recorded. The release of ciprofloxacin drug in vitro was reported to be 61.69 % during 24 h, and the final release rate was 82.17 %. Despite the biocompatibility and cell viability of 97.74 % and the biodegradability rate of 28.51 %, the StP-GOAgNWCip nanocomposite membrane can be introduced as a suitable candidate for wound dressing.

Healing double vacancy defects on graphene: reconstruction by C2 adsorption.

Graphene has emerged as an exciting material because of its widespread applications resulting from its unique properties. Nano-scale engineering of graphene's structure is one of the most active research areas aimed at introducing functionalities to improve the performance or endow the graphene lattice with novel properties. In this regard, conversion between the hexagon and non-hexagon rings becomes an exciting tool to tune the electronic structure of graphene due to the distinct electronic structure and functionalities induced in graphene by each type of ring. This Density Functional Theory (DFT) study is an in-depth look at the adsorption-induced conversion of pentagon-octagon-pentagon rings to hexagon rings, and systematically investigates the possibility of the conversion of pentagon-octagon-pentagon rings to pentagon-heptagon pair rings. Moreover, the bottlenecks for these atomic-level conversions in the lattice structure of graphene and the influence of heteroatom doping on the mechanisms of these transformations are established.

Multi-responsive on-demand drug delivery PMMA-co-PDEAEMA platform based on CO2, electric potential, and pH switchable nanofibrous membranes.

This study investigated the release characteristics of curcumin (CUR)-loaded switchable poly(methyl methacrylate)-co-poly(N,N-diethylaminoethyl methacrylate) (PMMA-co-PDEAEMA) membranes following the application of various stimuli, as well as the platform's applicability in wound dressing and tissue engineering applications. The free-radical polymerization method was used to synthesize the PMMA-co-PDEAEMA copolymer. The drug-loaded nanofibrous membrane with electric potential (EP)-, CO2-, and pH-responsive properties was developed by the electrospinning of PMMA-co-PDEAEMA and CUR. The resulted structure was characterized by a scanning electron microscope (SEM) coupled with X-ray energy dispersive spectroscopy and wide-angle X-ray scattering measurements. The release characteristics of the CUR-loaded wound covering were analyzed in various simulated environments at varying voltages, alternated CO2/N2 gas bubbling, and at two different pH values; the results demonstrated high drug release controllability. Loaded CUR displayed high stability and better solubility compared with free CUR. The CUR-loaded tissue also exhibited high antibacterial activity against Escherichia coli and staphylococcus aureus bacteria. In addition, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay depicted high biocompatibility of up to 95% in the CUR-loaded membrane. This platform could be a promising candidate for usage in tissue engineering and medical applications such as targeted drug delivery, biodetection, reversible cell capture-and-release systems, and biosensors.

Improving Nonenzymatic Biosensing Performance of Electrospun Carbon Nanofibers decorated with Ni/Co Particles via Oxidation.

Nonenzymatic biosensors do not require enzyme immobilization nor face degradation problem. Hence, nonenzymatic biosensors have recently attracted growing attention due to the stability and reproducibility. Here, a comparative study was conducted to quantitatively evaluate the glucose sensing of pure/oxidized Ni, Co, and their bimetal nanostructures grown on electrospun carbon nanofibers (ECNFs) to provide a low-cost free-standing electrode. The prepared nanostructures exhibited sensitivity (from 66.28 to 610.6 µA mM-1 cm-2), linear range of 2-10 mM, limit of detection in the range of 1 mM, and the response time (< 5 s), besides outstanding selectivity and applicability for glucose detection in the human serum. Moreover, the oxidizable interfering species, such as ascorbic acid (AA), uric acid (UA), and dopamine (DA), did not cause interference. Co-C and Ni-C phase diagrams, solid-state diffusion phenomena, and rearrangement of dissolved C atoms after migration from metal particles were discussed. This study undoubtedly provides new prospects on the nonenzymatic biosensing performance of mono-metal, bimetal, and oxide compounds of Ni and Co elements, which could be quite helpful for the fabrication of biomolecules detecting devices.

Investigating the structure of the product of graphene oxide reaction with folic acid and chitosan: density functional theory calculations.

Chitosan biopolymer was used to modify the level of graphene oxide. And the composite prepared from graphene oxide/chitosan, due to its favorable physical and chemical properties, have been used as a drug delivery system. In this study, the adsorption of Folic acid on the carrier was investigated using density functional theory (DFT). The geometry optimizations, electronic structures, and gas-phase properties of widely applicable graphene (G), graphene oxide (GO), chitosan (CS), folic acid (FA), GO-CS and GO-CS-FA were investigated using DFT. The studied molecules are based on graphene oxide. In GO-CS, DFT calculation show that two Chitosan connected to the GO molecule on both opposite sides, so that two Chitosan have maximum distance from each other. Finally, the electronic structure of FA was obtained with this molecule calculated and discussed. The interaction of hydrogen bonds in the most stable pair formers between molecules were determined. Furthermore, the hydrogen bonds were studied by atom in molecules natural bond orbital analyses.Communicated by Ramaswamy H. Sarma.

Photo-induced green synthesis of bimetallic Ag/Pd nanoparticles decorated reduced graphene oxide/nitrogen-doped graphene quantum dots nanocomposite as an amperometric sensor for nitrite detection.

The present study introduces a novel nanocomposite based on reduced graphene oxide, nitrogen-doped graphene quantum dots, and palladium and silver nanoparticles (rGO/NGQD/AgPd) as an electrocatalyst toward nitrite oxidation reaction. Metal nanoparticles were prepared via a green one-pot photochemical reduction procedure utilizing UV light and NGQD simultaneously as a reducing and directing agent. Formation of the nanocomposite was thoroughly demonstrated by the FT-IR, XRD, Raman, XPS, FE-SEM, and TEM characterization tests. Various electrochemical tests evaluated the efficiency of the prepared sensing platform on the surface of a gold working electrode. Sensitivity and limit of detection (LOD) were calculated to be 0.854 µA.µM-1.cm-2 and 0.052 µM, respectively, from the chronoamperometry data. Finally, the proposed sensor was successfully applied for the determination of nitrite ions in river and mineral water samples as natural water sources.

Coating Cellulosic Material with Ag Nanowires to Fabricate Wearable IR-Reflective Device for Personal Thermal Management: The Role of Coating Method and Loading Level.

Textiles coated with silver nanowires (AgNWs) are effective at suppressing radiative heat loss without sacrificing breathability. Many reports present the applicability of AgNWs as IR-reflective wearable textiles, where such studies partially evaluate the parameters for practical usage for large-scale production. In this study, the effect of the two industrial coating methods and the loading value of AgNWs on the performance of AgNWs-coated fabric (AgNWs-CF) is reported. The AgNWs were synthesized by the polyol process and applied onto the surface of cotton fabric using either dip- or spray-coating methods with variable loading levels of AgNWs. X-ray diffraction, scanning electron microscopy (SEM), infrared (IR) reflectance, water vapor permeability (WVP), and electrical resistance properties were characterized. The results report the successful synthesis of AgNWs with a 30 µm length. The results also show that the spray coating method has a better performance for reflecting the IR radiation to the body, which increases with a greater loading level of the AgNWs. The antibacterial results show a good inhibition zone for cotton fabric coated by both methods, where the spray-coated fabric has a better performance overall. The results also show the coated fabric with AgNWs maintains the level of fabric breathability similar to control samples. AgNWs-CFs have potential utility for cold weather protective clothing in which heat dissipation is attenuated, along with applications such as wound dressing materials that provide antibacterial protection.

Synthesis of wearable and flexible NiP0.1-SnOx/PANI/CuO/cotton towards a non-enzymatic glucose sensor.

Ni-SnOx, PANI and CuO nanoparticles were synthesized on cotton fabric through chemical methods to make a new flexible high-performance non-enzymatic glucose sensor. FESEM, XRD, XPS, EDS and ATR analysis were employed to characterize the structure and the morphology of the nanomaterials. The high electrochemical performance of nickel and copper oxide and hydroxide on a conductive template leads to fabrication of a wearable and flexible cotton electrode with an excellent electrocatalytic activity to oxidize glucose. This hybrid system on the fabric as an electrode indicates a detection limit of 130 nM with wide linear range of 0.001-10 mM. The sensitivity was measured to be 1625 and 1325 µA mM-1 cm-2 for the ranges of 0.001-1 and 1-10 mM, respectively. Long-term stability, appropriate selectivity and reusability for many times make possibility for utilizing the fabricated sensor in the practical applications. The fabric is a wide linear range electrode with low detection limit to sense glucose concentration in the body fluids as well as the human blood that can be presumably suggested for designing other similar flexible types of sensor.

Investigation of the effects of starch on the physical and biological properties of polyacrylamide (PAAm)/starch nanofibers.

Here, we report the development of a new polyacrylamide (PAAm)/starch nanofibers' blend system and highlight its potential as substrate for efficient enzyme immobilization. PAAm was synthesized and blended with starch. The final blend was then electrospun into nanofibers. The response surface methodology was used to analyze the parameters that control nanofiber's diameter. Electrospun mat was then modified either by cross-linking or phytase immobilization using silane coupling agent and glutaraldehyde chemistry. Physico-chemical properties of blends were investigated using spectroscopic and thermal studies. The evaluation of immobilized enzyme kinetics on both pure and the starch blended PAAm nanofibers was performed using Michaelis-Menten kinetic curves. Fourier transform infrared spectroscopy results along with differential scanning and X-ray diffraction confirmed that blending was successfully accomplished. TGA analysis also demonstrated that the presence of starch enhances the thermal degradability of PAAm nanofibers. Finally, it was shown that addition of starch to PAAm increases the efficacies of enzyme loading and, therefore, significantly enhances the activity as well as kinetics of the immobilized enzyme on electrospun blend mats.

Introducing a highly dispersed reduced graphene oxide nano-biohybrid employing chitosan/hydroxyethyl cellulose for controlled drug delivery.

In this research, an attempt was made to stabilize reduced graphene oxide (rGO) in all pH ranges, incorporating both chitosan (CS) and hydroxyethyl cellulose (HEC) to make a proper drug carrier with suitable stability and drug release behaviour. The stability of rGO-CS-HEC nanohybrid was assessed using field emission scanning electron microscopy (FE-SEM), ultraviolet-visible spectroscopy (UV-Vis) and Zeta potential measurements. Results depicted that the novel synthesized nanohybrid was stable in all pH ranges, due to the utilization of HEC, while without incorporation of this material, the rGO-CS nanohybrid aggregated at neutral and alkaline media, due to the ionic nature of chitosan. In addition, drug loading and release behaviour of folic acid (FA), as a model drug, was investigated to assess the role of chitosan on the release behaviour of FA from the rGO-CS-HEC nanohybrid in comparison with rGO-HEC and rGO-CS nanohybrids. It was proved that the resultant nanohybrid could release nearly 27% more FA than the rGO-HEC nanohybrid and only 9% lower than the rGO-CS nanohybrid during 120h. Moreover, the biocompatibility of the resultant nanohybrid was also checked to introduce the novel rGO-CS-HEC nanohybrid as a suitable candidate for drug delivery application.

A review of key challenges of electrospun scaffolds for tissue-engineering applications.

Tissue engineering holds great promise to develop functional constructs resembling the structural organization of native tissues to improve or replace biological functions, with the ultimate goal of avoiding organ transplantation. In tissue engineering, cells are often seeded into artificial structures capable of supporting three-dimensional (3D) tissue formation. An optimal scaffold for tissue-engineering applications should mimic the mechanical and functional properties of the extracellular matrix (ECM) of those tissues to be regenerated. Amongst the various scaffolding techniques, electrospinning is an outstanding one which is capable of producing non-woven fibrous structures with dimensional constituents similar to those of ECM fibres. In recent years, electrospinning has gained widespread interest as a potential tissue-engineering scaffolding technique and has been discussed in detail in many studies. So why this review? Apart from their clear advantages and extensive use, electrospun scaffolds encounter some practical limitations, such as scarce cell infiltration and inadequate mechanical strength for load-bearing applications. A number of solutions have been offered by different research groups to overcome the above-mentioned limitations. In this review, we provide an overview of the limitations of electrospinning as a tissue-engineered scaffolding technique, with emphasis on possible resolutions of those issues. Copyright © 2015 John Wiley & Sons, Ltd.

Graphene-oxide stabilization in electrolyte solutions using hydroxyethyl cellulose for drug delivery application.

Stabilization of graphene oxide (GO) in physiological solution is performed using hydroxyethyl cellulose (HEC) to make the resultant nanohybrid suitable for targeted drug delivery purposes. Short and long term stability of GO suspensions with different ionic strengths were assessed using ultraviolet-visible spectroscopy (UV-vis), atomic force microscopy (AFM) and zeta potential measurements. Results depicted that HEC effectively stabilized GO in electrolyte solutions and the mechanism of stabilization appeares to be depended on HEC content. Drug loading and release behavior of folic acid (FA) as a model drug, from GO-HEC nanohybrid were studied to assess its application in drug delivery systems. Results showed the nanohybrid could be highly loaded by folic acid. Moreover, HEC content in the nanohybrid played an important role in final application to make it applicable either as a carrier for controllable drug release or as a folate-targeted drug carrier. In addition, according to cytotoxicity results, the nanohybrid showed good biocompatibility which indeed confirms its potential application as a drug carrier.

Acetylcholinesterase immobilization on polyacrylamide/functionalized multi-walled carbon nanotube nanocomposite nanofibrous membrane.

In this work, polyacrylamide/multi-walled carbon nanotubes (MWCNT) solution is electrospun to nanocomposite nanofibrous membranes for acetylcholinesterase enzyme immobilization. A new method for enzyme immobilization is proposed, and the results of analysis show successful covalent bonding of enzymes on electrospun membrane surface besides their non-covalent entrapment. Fourier transform infrared spectroscopy, mechanical and thermal investigations of nanofibrous membrane approve successful cross-linking and enzyme immobilization. The enzyme relative activity and kinetic on both pure and nanocomposite membranes is investigated, and the results show proper performance of designed membrane to even improve the enzyme activity followed by immobilization compared to free enzyme. Scanning electron microscopy images show nanofibrous web of 3D structure with a low shrinkage and hydrogel structure followed by enzyme immobilization and cross-linking. Moreover, the important role of functionalized carbon nanotubes on final nanofibrous membrane functionality as a media for enzyme immobilization is investigated. The results show that MWCNT could act effectively for enzyme immobilization improvement via both physical (enhanced fibers' morphology and conductivity) and chemical (enzyme entrapment) methods.