Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 9 de 9
Filter
2.
J Mass Spectrom ; 52(5): 290-295, 2017 05.
Article in English | MEDLINE | ID: mdl-28251728

ABSTRACT

The use of sweeteners extracted from leaves of the plant species Stevia rebaudiana is increasing worldwide. They are recognized as generally recognized as safe by the US-FDA and approved by EU-European Food Safety Authority, with some recommendation on the daily dosage that should not interfere with glucose metabolism. The results presented here introduce an easy analytical approach for the identification and assay of Stevia sweeteners in commercially available soft drink, based on liquid chromatography coupled to tandem mass spectrometry, using a natural statin-like molecule, Brutieridin, as internal standard. Copyright © 2017 John Wiley & Sons, Ltd.


Subject(s)
Beverages/analysis , Diterpenes, Kaurane/analysis , Glucosides/analysis , Stevia/chemistry , Sweetening Agents/chemistry , Chromatography, High Pressure Liquid/methods , Plant Extracts/chemistry , Plant Leaves/chemistry , Tandem Mass Spectrometry/methods
3.
Minerva Anestesiol ; 80(6): 645-54, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24280819

ABSTRACT

BACKGROUND: The possibility of outlining a risk profile for perioperative blood transfusion of cirrhotic patients submitted to hepatic resection can help to rationalize transfusion policy. METHODS: Data from 323 hepatic resections, performed in cirrhotic patients, were reviewed. Bootstrap and a leave-one-out logistic regressions were applied to test the accuracy of available risk scores for peri-operative transfusion identified from PubMed search of the last 20 years, to refine them, and to provide internal validation for present results. RESULTS: One-hundred-six patients (32.8%) required blood transfusions during either intra- and/or postoperative. The predictive accuracy of three identified risk scores was poor with the area under receiver operating characteristics (AUROC) curves <0.70 in all cases. Tumor diameter, hemoglobin and presence of coronary artery disease were confirmed, in the present cohort, as predictors of blood transfusion together with serum albumin and bilirubin. The leave-one-out logistic regression results in an AUROC of 0.80, and of 0.79 for internal validation, significantly higher than that of the three scores tested (P<0.001). A Maximal Surgical Blood Order Schedule stratification was proposed. CONCLUSION: The risk profile for transfusion of cirrhotic patients undergoing hepatectomy can be better assessed with a model that combines already known clinical factors and hepatic function indexes.


Subject(s)
Blood Transfusion/methods , Hepatectomy/methods , Liver Cirrhosis/surgery , Perioperative Care/methods , Aged , Blood Loss, Surgical , Cohort Studies , Female , Humans , Male , Middle Aged , Reproducibility of Results , Risk Assessment
4.
J Phys Chem A ; 117(39): 9574-7, 2013 Oct 03.
Article in English | MEDLINE | ID: mdl-23461383

ABSTRACT

The A(2)Πg ← X(2)Πu electronic transition (4800-6000 Å) of triacetylene cation was measured in an ion trap, where the vibrational and rotational degrees of freedom were equilibrated to 25 K. The rotational profile of the origin band is predicted by a collisional-radiative rate model under conditions expected in diffuse interstellar clouds. Variation in the density of the surrounding gas, rotational temperature, and velocity dispersion are taken into account.

5.
Minerva Anestesiol ; 78(10): 1109-16, 2012 Oct.
Article in English | MEDLINE | ID: mdl-23059515

ABSTRACT

BACKGROUND: Currently, online social media have become increasingly popular and can provide the opportunity to provide and acquire information regarding knowledge and attitudes toward organ donation and transplantation. To evaluate participants' knowledge about organ donation, information sources and donation principles, an on-line survey was distributed through social network in Italy. METHODS: 10584 persons were invited to respond to the questionnaire, the response rate was 22.8% and a total of 2258 complete responses were analyzed. RESULTS: The majority of participants were in favour of organ donation (94.9%), but this proportion decreased when asking for consent to donation of a family member's organs (75.2%; P<0.001). Internet represented a considerable proportion of information sources (37.2%), that were much less frequently represented by family doctors (5.6%) and school education (18.6%). Conversely, 68.5% of participants think that family doctors should provide information regarding donation and 81.9% think schools should also provide such education (P<0.001). A good knowledge about donation principles was the main factor associated with a positive attitude toward donation (P<0.001). CONCLUSION: Efforts must be aimed at involving schools and family doctors in education about donation; the use of social networks can represent a way of improving such knowledge.


Subject(s)
Social Support , Tissue and Organ Procurement/statistics & numerical data , Adolescent , Adult , Aged , Attitude , Brain Death , Child , Coma , Female , Health Knowledge, Attitudes, Practice , Health Surveys , Humans , Italy/epidemiology , Male , Middle Aged , Organ Transplantation , Persistent Vegetative State , Surveys and Questionnaires , Young Adult
6.
J Phys Chem A ; 115(25): 6878-81, 2011 Jun 30.
Article in English | MEDLINE | ID: mdl-21413774

ABSTRACT

The origin band in the electronic transition to the dipole bound excited state of C(5)H(D)(-) anions was measured using two-color photodetachment spectroscopy. The rotational analysis of the partially resolved contour is consistent with a linear structure of the anion in both the ground X(3)Σ(-) and excited A(3)Π electronic states, in contrast with an earlier interpretation. The following spectroscopic constants are inferred for C(5)H(-): T(00) = 19248.0(1), B' = 0.0835(1), B'' = 0.0826(2), A'(SO) = -11.96(1), λ(SS)' = 1.97(8) λ(SS)'' = 0.24(15). Ab initio calculations at the UHF-MP2 level support the conclusion that C(5)H(-) is linear in the ground state. The experimentally determined ground state rotational constant can be used in the search for the millimeter wave spectrum of C(5)H(-).

7.
Altern Lab Anim ; 29(5): 601-11, 2001.
Article in English | MEDLINE | ID: mdl-11604101

ABSTRACT

A new, mechanistically based, in vitro strategy involving Balb/c 3T3 clone A 31-1-1 mouse embryo fibroblasts has been proposed for the determination of the carcinogenic potential of inorganic chemicals, in order to establish priority of metal compounds to be tested and, whenever possible, to compare the in vitro results with the corresponding in vivo data. As a first step in this research, this study reports on the cytotoxic effects of 58 metal compounds in the Balb/3T3 cell line. After harmonisation and standardisation of the Balb/3T3 protocol, cells were exposed for 72 hours to a fixed dose (100 microM) of 58 individual compounds. The cytotoxicity induced by some metal compounds was found to be related to their chemical form (for example, Cr(NO(3))(3) and Na(2)CrO(4)), suggesting that the Balb/3T3 cell line is a valuable cellular model in relation to this aspect of metal speciation. The results of the systematic study on the metal-induced cytotoxic effects in the Balb/3T3 cell line could be arbitrarily classified into three groups according to the degree of cytotoxicity. Group I includes 26 species that induced no observable effect or only a slight cytotoxic effect; Group II includes 13 metal compounds that exhibited an obvious degree of cytotoxicity; and Group III includes 19 metal species that displayed a strong cytotoxic response. Metal compounds of Groups II and III are considered to be of the highest priority for setting of dose-effect relationships for a subsequent in vitro study on metal-induced concurrent cytotoxicity and morphological transformation in the Balb/3T3 cell line.


Subject(s)
3T3 Cells/drug effects , Animal Testing Alternatives , Carcinogenicity Tests , Cell Death/drug effects , Metals/toxicity , Animal Testing Alternatives/methods , Animal Testing Alternatives/standards , Animals , Blood , Culture Media , Hydrogen-Ion Concentration , Mice , Mice, Inbred BALB C , Neutral Red/metabolism , Reproducibility of Results
9.
J Mass Spectrom ; 35(3): 461-7, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10767778

ABSTRACT

The composition of the equilibrium between aldehydes and hemiacetals was evaluated by ionspray ionization tandem mass spectrometry. The methanolic extracts of virgin olive oil from Carolea and Coratina cultivars contain a complex mixture of hydroxytyrosol (3) and tyrosol (4) derivatives (5, 6) characterized by the presence of aldehyde moieties in equilibrium with their hydrated form (8) or with the corresponding methanol hemiacetals 9 and 10. The equilibrium was frozen by droplet evaporation, thus allowing the structure determination of each component. The formation of the decarboxylated species 11-14, reported previously, was not observed.

SELECTION OF CITATIONS
SEARCH DETAIL
...