ABSTRACT
Clostridium chauvoei is the pathogenic agent for blackleg, a toxinfection disease in bovine and small ruminants, always lethal and involving considerable economic losses. Some bacteriological, biochemical, immunological studies permitted to isolate identify the major soluble antigenic protein of this bacteria. It's a protein fragment of 70 kDa weight, the 19 fraction, excreted by the bacteria in a suitable culture medium. The 19 fraction of extracellular medium leads to antibodies production on guinea pigs revealed by the ELISA/antibody test.
Subject(s)
Antigens, Bacterial/isolation & purification , Bacterial Proteins/immunology , Clostridium chauvoei/immunology , Animals , Antibodies, Bacterial/biosynthesis , Antibodies, Bacterial/immunology , Antigens, Bacterial/analysis , Bacterial Proteins/analysis , Bacterial Proteins/isolation & purification , Culture Media , Culture Media, Conditioned , Enzyme-Linked Immunosorbent Assay , Flagella/immunology , Guinea Pigs , Immunization , Mice , Molecular Weight , Peptide Fragments/analysis , Peptide Fragments/immunology , Peptide Fragments/isolation & purification , SenegalABSTRACT
The authors report the results of sero-epidemiological monitoring of Zebu, Diakoré and Ndama cattle in the Sahel, Niayes and North Guinea zones, respectively. Calves aged 0-3 or 3-6 months were subjected to blood sampling every three months for a period of 20 months. In the Niayes and North Guinea zones, the immunofluorescence test showed that about 70% of 0-1 month old calves reacted positively to the Cowdria ruminantium antigen, whereas the prevalence was 92% between 3 and 6 months. After the age of 6 months, the results were 100% positive. In the Sahelian area, the prevalence of the infection was null. Results obtained with a sample of about 100 adult cattle from the Sahel, Niayes, North Sudan and North Guinea zones, showed a good correlation between positivity rates and the size of Amblyomma variegatum populations.
