1.
Acta Trop
; 63(2-3): 185-9, 1997 Feb.
Article
in English
| MEDLINE
| ID: mdl-9088432
Subject(s)
Anti-Infective Agents/therapeutic use , Antimalarials/therapeutic use , Dapsone/therapeutic use , Malaria, Falciparum/drug therapy , Proguanil/analogs & derivatives , Pyrimethamine/therapeutic use , Sulfadoxine/therapeutic use , Child , Child, Preschool , Drug Resistance, Microbial , Drug Therapy, Combination , Humans , Infant , Malaria, Falciparum/epidemiology , Proguanil/therapeutic use , Tanzania/epidemiology
2.
Trans R Soc Trop Med Hyg
; 90(6): 678-80, 1996.
Article
in English
| MEDLINE
| ID: mdl-9015515
ABSTRACT
A nested polymerase chain reaction was used to amplify a fragment of the gene for dihydrofolate reductase of Plasmodium falciparum containing codon 108, where a point mutation, causing a serine to asparagine change, occurs in pyrimethamine resistant parasites. The presence of the mutation was detected by restriction enzyme digestion. Parasites in blood samples collected from asymptomatic children before, and 3 weeks after, treatment with pyrimethamine-sulfadoxine or chlorproguanil-dapsone were analysed. Parasites in the samples taken at 3 weeks carried only the asparagine mutant.