High magnetic relaxivity in a fluorescent CdSe/CdS/ZnS quantum dot functionalized with MRI contrast molecules.

Light emitting semiconducting quantum dots show great promise as solar cells, optoelectronic devices and multimodal imaging probes. Here we demonstrate successful grafting of a thiol-functionalised GdIII MRI contrast agent onto the surface of core-multishell CdSe/CdS/ZnS quantum dots. The resulting nanoprobe exhibits intense photoluminescence and unprecedentedly large T1 relaxivity of 6800 mM-1 s-1 per nanoparticle due to secure implanting of ca. 620 magnetic centers per quantum dot unit.

Prevalence of Mycobacterium avium subsp. paratuberculosis fecal shedding in alpacas presented to veterinary hospitals in the United States.

BACKGROUND: The prevalence of Johne's disease in alpacas in the United States is unknown. The limits of polymerase chain reaction (PCR) detection of Mycobacterium avium subsp. paratuberculosis (MAP) in alpaca feces have not been determined. OBJECTIVES: To evaluate the use of PCR for MAP detection in alpaca feces; and to estimate the prevalence of MAP fecal shedding in alpacas presented to veterinary teaching hospitals. ANIMALS: Alpacas presenting to 4 US veterinary teaching hospitals from November 2009 to February 2011. METHODS: Prospective study. Ten dilutions of a wild MAP strain were added to negative alpaca feces and processed for MAP detection by means of a commercial real-time PCR (RT-PCR) assay, and cultured on Herrold's Egg Yolk Medium (HEYM) and liquid broth. The limits of detection for each method were determined. Fecal samples from alpacas admitted to the veterinary teaching hospitals during the study period were evaluated for MAP via PCR and HEYM. RESULTS: The lowest MAP dilution detectable via PCR was 243 MAP colony-forming units (CFU)/g of feces, at which concentration MAP growth was detectable on HEYM. Ten (6%; 95% confidence interval: 3-9%) of the 180 fecal samples collected were positive on PCR. CONCLUSIONS AND CLINICAL IMPORTANCE: Polymerase chain reaction can provide an accurate and rapid detection of MAP fecal shedding in alpacas; and the prevalence of MAP fecal shedding in hospitalized alpacas in 4 US veterinary teaching hospitals was 6%.

Antimicrobial activity of gallium nitrate against Mycobacterium avium subsp. paratuberculosis in neonatal calves.

BACKGROUND: Mycobacterium avium subsp. paratuberculosis (MAP), the agent of Johne's disease in cattle, is a facultative intracellular bacterium that is dependent on ferric iron for its survival and replication. Gallium (Ga), a trivalent semimetal that shares many similarities with ferric iron and functions as an iron mimic has been shown to have in vitro antimicrobial activity against several microorganisms, including MAP. OBJECTIVES: (1) To investigate the antimicrobial activity of Ga in calves experimentally infected with MAP; and (2) to monitor for potential adverse effects of Ga on calf health. ANIMALS: Twelve Holstein calves. METHODS: Randomized blind controlled experiment. Beginning at 10 days of age (study day 1), the experimental calves (n = 6) were treated with 20 mg/kg gallium nitrate daily for 45 days. On study days 4 and 5, all calves were challenged with a PO dose of a live field strain MAP. Treated calves were monitored daily for adverse effects. Calves were euthanized on study day 100, and 29 tissue samples and 1 fecal sample were collected from each calf. Samples were cultured for MAP by MGIT liquid culture system, Herrold's Egg Yolk Medium culture, or both. RESULTS: No adverse effects were observed in the treated calves. Treatment was associated with a significant reduction in MAP tissue burden when compared with control calves (P = .017). CONCLUSIONS AND CLINICAL RELEVANCE: Chemoprophylactic treatment of calves with Ga before and during the period of high susceptibility decreased MAP tissue colonization in experimentally infected neonatal calves.

Reliability of environmental sampling to quantify Mycobacterium avium subspecies paratuberculosis on California free-stall dairies.

The reliability of environmental sampling to quantify Mycobacterium avium ssp. paratuberculosis (MAP) based on collector and time was evaluated. Fecal slurry samples were collected using a standardized protocol simultaneously by 2 collectors of different experience levels. Samples were collected from 30 cow pens on 4 dairies every other day on 3 occasions while cow movements between pens were minimal. The 4 study herds had moderate MAP seroprevalence and were housed in free-stall dairies in central California. Results of testing the environmental samples for MAP using PCR and culture were strongly correlated. The reliability of environmental sampling simultaneously by different collectors as estimated by the intraclass correlation coefficient was excellent (81%) for PCR and good (67%) for culture and may justify comparison of quantitative results of samples collected by different investigators. The reliability of environmental sampling over a 5-d period was good (67 and 64% for PCR and culture results, respectively), which justifies the utility of environmental sampling to identify pens with a high MAP bioburden between routine cow pen changes on a dairy. Environmental sampling of free-stall pens using the standardized sampling protocol yielded comparable PCR and culture results across collectors with different experience levels and at different times within a 5-d period.

Neural correlates of timbre change in harmonic sounds.

Timbre is a major structuring force in music and one of the most important and ecologically relevant features of auditory events. We used sound stimuli selected on the basis of previous psychophysiological studies to investigate the neural correlates of timbre perception. Our results indicate that both the left and right hemispheres are involved in timbre processing, challenging the conventional notion that the elementary attributes of musical perception are predominantly lateralized to the right hemisphere. Significant timbre-related brain activation was found in well-defined regions of posterior Heschl's gyrus and superior temporal sulcus, extending into the circular insular sulcus. Although the extent of activation was not significantly different between left and right hemispheres, temporal lobe activations were significantly posterior in the left, compared to the right, hemisphere, suggesting a functional asymmetry in their respective contributions to timbre processing. The implications of our findings for music processing in particular and auditory processing in general are discussed.

Similarity, invariance, and musical variation.

Perceptual similarity underlies a number of important psychological properties of musical materials, including perceptual invariance under transformation, categorization, recognition, and the sense of familiarity. Mental processes involved in the perception of musical similarity may be an integral part of the functional logic of music composition and thus underly important aspects of musical experience. How much and in what ways can musical materials be varied and still be considered as perceptually related or as belonging to the same category? The notions of musical material, musical variation, perceptual similarity and invariance, and form-bearing dimensions are considered in this light. Recent work on similarity perception has demonstrated that the transformation space for a given musical material is limited by several factors ranging from degree of match of the values of auditory attributes of the events composing the sequences to their relations of various levels of abstraction and to the degree that the transformation respects the grammar of the musical system within which the material was composed. These notions and results are considered in the light of future directions of research, particularly concerning the role of similarity and invariance in the understanding of musical form during listening.

Feature dependence in the automatic identification of musical woodwind instruments.

The automatic identification of musical instruments is a relatively unexplored and potentially very important field for its promise to free humans from time-consuming searches on the Internet and indexing of audio material. Speaker identification techniques have been used in this paper to determine the properties (features) which are most effective in identifying a statistically significant number of sounds representing four classes of musical instruments (oboe, sax, clarinet, flute) excerpted from actual performances. Features examined include cepstral coefficients, constant-Q coefficients, spectral centroid, autocorrelation coefficients, and moments of the time wave. The number of these coefficients was varied, and in the case of cepstral coefficients, ten coefficients were sufficient for identification. Correct identifications of 79%-84% were obtained with cepstral coefficients, bin-to-bin differences of the constant-Q coefficients, and autocorrelation coefficients; the latter have not been used previously in either speaker or instrument identification work. These results depended on the training sounds chosen and the number of clusters used in the calculation. Comparison to a human perception experiment with sounds produced by the same instruments indicates that, under these conditions, computers do as well as humans in identifying woodwind instruments.

tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta.

The rice blast resistance (R) gene Pi-ta mediates gene-for-gene resistance against strains of the fungus Magnaporthe grisea that express avirulent alleles of AVR-Pita. Using a map-based cloning strategy, we cloned Pi-ta, which is linked to the centromere of chromosome 12. Pi-ta encodes a predicted 928-amino acid cytoplasmic receptor with a centrally localized nucleotide binding site. A single-copy gene, Pi-ta shows low constitutive expression in both resistant and susceptible rice. Susceptible rice varieties contain pi-ta(-) alleles encoding predicted proteins that share a single amino acid difference relative to the Pi-ta resistance protein: serine instead of alanine at position 918. Transient expression in rice cells of a Pi-ta(+) R gene together with AVR-Pita(+) induces a resistance response. No resistance response is induced in transient assays that use a naturally occurring pi-ta(-) allele differing only by the serine at position 918. Rice varieties reported to have the linked Pi-ta(2) gene contain Pi-ta plus at least one other R gene, potentially explaining the broadened resistance spectrum of Pi-ta(2) relative to Pi-ta. Molecular cloning of the AVR-Pita and Pi-ta genes will aid in deployment of R genes for effective genetic control of rice blast disease.

Direct interaction of resistance gene and avirulence gene products confers rice blast resistance.

Rice expressing the Pi-ta gene is resistant to strains of the rice blast fungus, Magnaporthe grisea, expressing AVR-Pita in a gene-for-gene relationship. Pi-ta encodes a putative cytoplasmic receptor with a centrally localized nucleotide-binding site and leucine-rich domain (LRD) at the C-terminus. AVR-Pita is predicted to encode a metalloprotease with an N-terminal secretory signal and pro-protein sequences. AVR-Pita(176) lacks the secretory and pro-protein sequences. We report here that transient expression of AVR-Pita(176) inside plant cells results in a Pi-ta-dependent resistance response. AVR-Pita(176) protein is shown to bind specifically to the LRD of the Pi-ta protein, both in the yeast two-hybrid system and in an in vitro binding assay. Single amino acid substitutions in the Pi-ta LRD or in the AVR-Pita(176) protease motif that result in loss of resistance in the plant also disrupt the physical interaction, both in yeast and in vitro. These data suggest that the AVR-Pita(176) protein binds directly to the Pi-ta LRD region inside the plant cell to initiate a Pi-ta-mediated defense response.

Perception of musical tension for nontonal orchestral timbres and its relation to psychoacoustic roughness.

Can tension in nontonal music be expressed without dynamic or rhythmic cues? Perceptual theories of tonal harmony predict that psychoacoustic roughness plays an important role in the perception of this tension. We chose a set of orchestrated chords from a nontonal piece and investigated listeners' judgments of musical tension and roughness. Paired comparisons yielded psychophysical scales of tension and roughness. Two experiments established distinct levels of these two attributes across chords. A model simulation reproduced the experimental roughness measures. The results indicate that nontonal tension could be perceived consistently on the basis of timbral differences and that it was correlated with roughness, the correlation being stronger as the perceptual salience of other attributes (such as high-pitched tones or tonal intervals) was reduced.

Two phase effects in roughness perception.

The respective influences of spectral and temporal aspects of sound in roughness perception are examined by way of phase manipulations. In a first experiment, the phase of the central component of three-component signals is shown to modify perceived roughness, for a given amplitude spectrum, regardless of whether it modifies the waveform envelope. A second experiment shows that the shape of the waveform envelope, for a given amplitude spectrum and a given modulation depth, also influences perceived roughness. We interpret both of these results by considering the envelope of an internal representation that is deduced from the physical signal by taking into account peripheral auditory processing. The results indicate that the modulation depth of such an internal representation is not the only determinant of roughness, but that an effect of temporal asymmetry is also to be taken into account.

Discrimination of musical instrument sounds resynthesized with simplified spectrotemporal parameters.

The perceptual salience of several outstanding features of quasiharmonic, time-variant spectra was investigated in musical instrument sounds. Spectral analyses of sounds from seven musical instruments (clarinet, flute, oboe, trumpet, violin, harpsichord, and marimba) produced time-varying harmonic amplitude and frequency data. Six basic data simplifications and five combinations of them were applied to the reference tones: amplitude-variation smoothing, coherent variation of amplitudes over time, spectral-envelope smoothing, forced harmonic-frequency variation, frequency-variation smoothing, and harmonic-frequency flattening. Listeners were asked to discriminate sounds resynthesized with simplified data from reference sounds resynthesized with the full data. Averaged over the seven instruments, the discrimination was very good for spectral envelope smoothing and amplitude envelope coherence, but was moderate to poor in decreasing order for forced harmonic frequency variation, frequency variation smoothing, frequency flattening, and amplitude variation smoothing. Discrimination of combinations of simplifications was equivalent to that of the most potent constituent simplification. Objective measurements were made on the spectral data for harmonic amplitude, harmonic frequency, and spectral centroid changes resulting from simplifications. These measures were found to correlate well with discrimination results, indicating that listeners have access to a relatively fine-grained sensory representation of musical instrument sounds.

The auditory continuity phenomenon: role of temporal sequence structure.

The auditory continuity phenomenon occurs when listening to a series of alternating high- and low-level tones: instead of perceiving this intermittence, listeners often report hearing a continuous tone upon which is superimposed a series of intermittent tones. The temporal limits to the perception of this phenomenon are investigated, as well as effects of task instructions on it. A loudness-matching paradigm developed previously [McAdams et al., J. Acoust. Soc. Am. 130, 1580-1591 (1998)] provided both an objective indication of this phenomenon and an indication of its strength. In the studies reported here, the phenomenon was observed reliably when the low-level tones were at least half the duration of the high-level tones: the greater the duration ratio between low-level and high-level tones, the stronger the phenomenon. Duty-cycle duration did not affect the strength of this phenomenon. It proved to be particularly robust as its strength was unaffected by task instructions, level of expertise, and repetition. A model is proposed in which adjusted levels depend on the relative levels of the high- and low-level tones weighted by relative duration and attentional focusing.

The functional anatomy of sound intensity discrimination.

The human neuroanatomical substrate of sound intensity discrimination was investigated by combining psychoacoustics and functional neuroimaging. Seven normal subjects were trained to detect deviant sounds presented with a slightly higher intensity than a standard harmonic sound, using a Go/No Go paradigm. Individual psychometric curves were carefully assessed using a three-step psychoacoustic procedure. Subjects were scanned while passively listening to the standard sound and while discriminating changes in sound intensity at four different performance levels (d' = 1.5, 2.5, 3.5, and 4.5). Analysis of regional cerebral blood flow data outlined activation, during the discrimination conditions, of a right hemispheric frontoparietal network already reported in other studies of selective or sustained attention to sensory input, and in which activity appeared inversely proportional to intensity discriminability. Conversely, a right posterior temporal region included in secondary auditory cortex was activated during discrimination of sound intensity independently of performance level. These findings suggest that discrimination of sound intensity involves two different cortical networks: a supramodal right frontoparietal network responsible for allocation of sensory attentional resources, and a region of secondary auditory cortex specifically involved in sensory computation of sound intensity differences.

Auditory continuity and loudness computation.

Sequences composed of alternating bursts of different levels with no silences separating them can give rise to a perception of a continuous sound upon which is superimposed an intermittent stream. These experiments sought to determine how the perceived loudness of the intermittent stream depends on the level difference between higher-level and lower-level bursts in the sequence in cases in which continuity is either heard or not heard. In the main experiment, listeners were asked to adjust the level of continuous or intermittent comparison sequences to match the loudness of components that appeared to be either continuous or intermittent in an alternating-level reference sequence, thus urging them to focus on the two-stream percept. Loudness matches of continuous comparison stimulus were close to physical levels of the lower-level bursts, whereas matches of the intermittent comparison stimulus were well below the physical levels of higher-level bursts. These results are discussed in terms of Bregman's [Auditory Scene Analysis (MIT, Cambridge, MA, 1990)] "old-plus-new" hypothesis: The loudness of the intermittent stream should result from the subtraction of the lower level from the higher level under the assumption that the higher-level burst represents a simultaneous mixture of sounds including the continuation of the lower-level burst. Additional experiments verified that, in the absence of the continuity phenomenon, matched levels were very close to the physical levels and that matches to fixed-level continuous and intermittent sequences were precise. The matching results from the main experiment support predictions of neither classical loudness models that do not take auditory organization processes into account nor schema-based models that presume a selection of information from the higher-level burst that does not affect the perceptual content of this burst. The matched levels fell between predictions of models based on subtraction of acoustic pressure and acoustic power, but were very different from subtraction of loudness measured in sones, suggesting that loudness is computed subsequent to auditory organization processes.

The representation of auditory source characteristics: simple geometric form.

Two experiments examined listeners' ability to discriminate the geometric shape of simple resonating bodies on the basis of their corresponding auditory attributes. In cross-modal matching tasks, subjects listened to recordings of pairs of metal bars (Experiment 1) or wooden bars (Experiment 2) struck in sequence and then selected a visual depiction of the bar cross sections that correctly represented their relative widths and heights from two opposing pairs presented on a computer screen. Multidimensional scaling solutions derived from matching scores for metal and wooden bars indicated that subjects' performance varied directly with increasing differences in the width/height (W/H) ratios of both sets of bars. Subsequent acoustic analyses revealed that the frequency components from torsional vibrational modes and the ratios of frequencies of transverse bending modes in the bars correlated strongly with both the bars' W/H ratios and bar coordinates in the multidimensional configurations. The results suggest that listeners can encode the auditory properties of sound sources by extracting certain invariant physical characteristics of their gross geometric properties from their acoustic behavior.

Isolation, genetic diversity, and geographic distribution of Bayou virus (Bunyaviridae: hantavirus).

Bayou hantavirus, previously implicated in human hantavirus pulmonary syndrome in Louisiana, was isolated from a rice rat (Oryzomys palustris) captured in Georgia. The presence of antibody among rice rats captured throughout the southeastern United States and the extent of diversity among the genetic variants of Bayou viruses suggest that the rice rat is the most likely natural reservoir of the virus and that both virus and host have probably co-evolved for some years.

Organization and discrimination of repeating sound sequences by newborn infants.

A study was conducted to determine whether newborn infants organize auditory streams in a manner similar to that of adults. A series of three experiments investigated the ability of 3- to 4-day-old infants to discriminate repeated rising and falling four-tone sequences in two configurations of source timbre and spatial position. It was hypothesized that if the sequences were organized into two auditory streams on the basis of timbre and spatial position, one of the configurations should be discriminable from its reversal while the other should not. The sequences were tested with different pitch and temporal intervals separating the tones. Sequences were discriminated for the first configuration by adults at both fast tempo/small interval and slow tempo/large interval combinations, while only the latter was discriminated by newborns as measured with a non-nutritive high-amplitude sucking paradigm. Neither adults nor infants could discriminate the sequence reversals for the second configuration. The results suggest that newborn infants organize auditory streams on the basis of source timbre and/or spatial position. They also suggest that newborns have limits in temporal and/or pitch resolution when discriminating tone sequences.

Perceptual attenuation of nonfocused auditory streams.

The aim of this study was to measure the perceptual attenuation, measured in decibels, resulting from the focusing of attention on one stream within a multistream auditory sequence. The intensity of a nonfocused stream was increased until the accuracy of detecting a temporal irregularity in this stream was the same as in a focused stream. Eight subjects were required to detect a temporal irregularity created by delaying or advancing one tone which could be situated in one of three temporally regular streams played simultaneously to create a multistream sequence. The three streams differed in tempo and frequency. Subjects' attention was focused on one of the streams by preceding the multistream sequence with one of the single streams (a cue). We first established the size of temporal irregularity detected at a 90% level in cued streams, confirming that subjects were able to focus on one particular stream. Second, an irregularity of this size was not detected above chance level in noncued streams, demonstrating that listeners focus only on the cued stream. Third, for 5 subjects, a 15-dB increase in the level of one of the noncued streams was necessary to bring detection up to that found in the cued streams. This gain provides an equivalent measure of the perceptual attenuation of nonfocused streams. For 3 other subjects, detection in the noncued stream remained at chance performance whatever the level. For all subjects, detection in the cued stream decreased slightly as the level of the noncued stream increased. We conclude that the attenuation of nonfocused auditory streams can attain as much as 15 dB, at least for some subjects.

The role of auditory beats induced by frequency modulation and polyperiodicity in the perception of spectrally embedded complex target sounds.

The contribution of auditory beats to the perception of target sounds differing from an interfering background by their frequency modulation (FM) pattern or by a difference in fundamental frequency (F0) was investigated. On each trial, test sounds composed of a single, second-order formant were embedded in harmonic backgrounds and presented in successive intervals. The center frequencies of these "normal" formants differed across intervals. Subjects were to decide which interval contained the test formant with a center frequency matching that of an isolated target formant presented before each test stimulus. Matching thresholds were measured in terms of the width of modulation for FM stimuli or the mistuning of the F0's of unmodulated test formants relative to that of the background. Beats may have allowed the identification of the spectral region of the target in both experiments. To reduce interactions between test and background components, matching thresholds were measured for "flat" formants composed of two or three equal-amplitude components embedded in a harmonic background in which components corresponding to those of test formants were absent. These measures were repeated with the addition of a pink noise floor. Matching was still possible in all cases, though at higher thresholds than for normal formants. Computer simulations suggested that the modulation depth of envelope fluctuations within auditory channels played a significant role in the matching of target sounds when their components were mixed in the same frequency region with those of an interfering sound, but not when the target and background components were separated by as much as 250 Hz, the F0 of the stimulus.