Subject(s)
Cadaver , Ethnicity , Kidney Transplantation , Actuarial Analysis , Black People , Graft Survival , Histocompatibility , Humans , Mexico/ethnology , United States , West Indies/ethnology , White PeopleABSTRACT
In vitro inhibition of marrow granulopoiesis was produced by a well-characterized human neuroblastoma cell line (CHP 134). A standard double layer, semi-solid agar system was employed in the experiments. The inhibition was present whether the neuroblastoma cells were mixed with the marrow cells or whether they were separated in a contiguous agar layer. Irradiation of the neuroblastoma cells lessened the inhibitory effect but did not eradicate it. Medium conditioned by the neuroblastoma cells had a mild, but not statistically significant, suppressive effect upon granulopoiesis. Additional studies to define the precise mechanism of suppression are underway.
Subject(s)
Colony-Forming Units Assay , Granulocytes/cytology , Hematopoiesis , Neuroblastoma/pathology , Cell Division , Cell Line , Culture Media , Humans , In Vitro TechniquesSubject(s)
HLA Antigens , Neuroblastoma/immunology , Absorption , Cells, Cultured , Child , Child, Preschool , Cytotoxicity, Immunologic , Edetic Acid/pharmacology , Female , Fibroblasts , Gene Frequency , Haploidy , Humans , Infant , Male , Neuraminidase/pharmacology , Neuroblastoma/genetics , Phenotype , Trypsin/pharmacologyABSTRACT
Rosettes of platelets around granulocytes-platelet satellitism-previously described in Behcet's syndrome led to the discovery of a case that may have been 80 years in duration. A strong relationship between calcium ions and the phenomenon was suggested by its specificity in edetic-acidanticoagulated blood, and the subsequent migration of platelets on supravital preparation from around neutrophils upon addition of 0.2 M calcium chloride to heparinized EDTA-treated blood. Plasma from the patient was able to cause the phenomenon with donor granulocytes and platelets. Platelet agglutinins were also demonstrable. Specificity in Behcet's syndrome is possible, but remains unproved.
Subject(s)
Embolism/chemically induced , Kidney Transplantation , Postoperative Complications , Starch/adverse effects , Animals , Biopsy, Needle , Blood Coagulation Tests , Capillaries , Child , Filtration , Gloves, Surgical , Humans , In Vitro Techniques , Kidney Glomerulus/blood supply , Kidney Glomerulus/pathology , Microscopy, Electron , Platelet Aggregation , Rabbits , Thrombosis/etiology , Thrombosis/prevention & control , Transplantation, Homologous , Zea maysSubject(s)
Antibody Formation , Antigens , Vibrio/immunology , Animals , Antibody Specificity , Antibody-Producing Cells , Antigens, Bacterial , Bacteriolysis , Hemagglutination Tests , Hemolytic Plaque Technique , Immune Sera , Immunity, Cellular , Lipopolysaccharides , Lymph Nodes/immunology , Lymphocytes/immunology , Male , Mice , Mice, Inbred Strains , Polysaccharides, Bacterial , Spleen/cytology , Spleen/immunologyABSTRACT
Mice were infected with Friend leukemia virus and later immunized with either Vibrio cholerae vaccine or sheep erythrocytes. The primary antibody response to the bacteria (as judged by the number of plaque-forming cells) was slightly enhanced by the viral infection, whereas the response to sheep erythrocytes was inhibited. The difference appeared due to sensitization of mice to antigens crossreacting with those of sheep erythrocytes; no natural immunity to V. cholerae is detectable. However, the response of mice infected with Friend leukemia virus to a secondary challenge with the cholera bacteria was markedly inhibited. Even though the number of plaque-forming cells during the primary response was not reduced, accumulation of the cells in distinct splenic foci was suppressed. These results suggest that the effect of Friend leukemia virus on immunocompetent cells is selective. The immune response appears to be susceptible to leukemia virus-induced immunosuppression only when there has been a previous stimulation of immunocytes by antigen.
