ABSTRACT
Confocal microscopy has facilitated measurement of stained lipid volume in Lepeophtheirus salmonis copepodid larvae. Quantity of lipid, location and morphology of vesicles may allow an estimate of age and viability.
Subject(s)
Copepoda/physiology , Lipids/analysis , Microscopy, Confocal , Aging , Animals , Copepoda/chemistry , Ectoparasitic Infestations/parasitology , Ectoparasitic Infestations/veterinary , Fish Diseases/parasitology , Larva/chemistry , Salmon/parasitologyABSTRACT
Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase-based immunoassay, and Western blot.
