ABSTRACT
PROBLEM: Isolation of viable cervical lymphocyte populations and characterization of their function in healthy tissue is necessary to understand immunity in the genital tract. METHODS: Normal, cervical tissue was digested using a multi-enzymatic digestion procedure. Lymphocytes were characterized using FACS analysis and ELISPOT analysis for immunoglobulin secreting cells. RESULTS: Following the digestion procedure, 0.16 x 10(6) +/- 0.8 cells/g of tissue with a viability of 90-98% were isolated from normal cervical tissue. FACS analysis determined that B lymphocytes were the predominant cell type in normal cervical tissue representing a significantly higher percentage than that found in peripheral blood (P = 0.015). T lymphocytes and NK cells represented a significantly lower percentage than that found in peripheral blood (P = 0.0001 and 0.026, respectively). The largest percentage of immunoglobulin secreting cells isolated were secreting IgG followed by IgA. A limited number of IgM secreting cells were detected. IgA2 secreting cells represented 34.46 +/- 4.6% of the total number of IgA plasma cells. CONCLUSION: These studies represent the first analysis of viable mononuclear cells isolated from normal cervical tissue. The results form a baseline from which it will now be possible to compare changes that occur at the cervical squamocolumnar junction in response to infection or neoplasia.
Subject(s)
Cervix Uteri/immunology , Immunoglobulins/biosynthesis , Lymphocyte Subsets/classification , Adult , Antibody-Producing Cells/classification , Cell Separation , Cervix Uteri/metabolism , Female , Humans , Immunophenotyping , Leukocytes, Mononuclear/classification , Leukocytes, Mononuclear/immunology , Leukocytes, Mononuclear/metabolism , Lymphocyte Count , Lymphocyte Subsets/immunology , Lymphocyte Subsets/metabolism , Middle AgedABSTRACT
Soil properties and retention mechanisms affecting the relative mobility of aniline, benzoic acid, and toluene in Cecil sandy loam (clayey, kaolinitic, thermic Typic Hapludults), Holdredge silt loam (fine-silty, mixed, mesic Typic Argiustolls), Sharpsburg silty clay loam (fine, montmorillonitic, mesic Typic Argiudolls), and Valentine fine sand (mixed, mesic Typic Ustipsamments) were delineated in laboratory studies. The effect of the synthetic organic compounds (SOCs) was also determined on effective organic campounds (SOCs) was also determined on effective cation exchange capacity (ECEC) of the soils. Benzoic acid was most mobile of the SOCs but was retained in the Cecil soil by hydrogen bonds to Fe oxides. Toluene was least mobile and probably was hydrophobically sorbed to all soils. Aniline was electrostatically retained in protonated form to organic and inorganic exchange sites of the soils and reduced the ECEC of aniline-treated Holdredge and Sharpsburg soils.
ABSTRACT
Dispersion, dissipation, and efficacy of gas and gel formulations of methyl bromidechloropicrin (202, 269, 336, and 403 kg/ha) on nematodes and weeds on tomato were studied in field plots. Concentrations of methyl bromide and chloropicrin 4 hr after soil treatment were greater at a depth of 15 cm than at 30, 45, or 60 cm. The concentrations of both chemicals decreased with lower doses, greater depths, and longer times after application. The gel formulation was more persistent than the gas formulation at both 336 and 403 kg/ha at depths of 30 and 45 cm, especially 24 and 36 hr after chemical application. Plant growth and yield were improved when nematodes and weeds were controlled.
