A Novel Subcluster of Closely Related Bacillus Phages with Distinct Tail Fiber/Lysin Gene Combinations.

Bacteriophages (phages) are the most numerous entities on Earth, but we have only scratched the surface of describing phage diversity. We isolated seven Bacillus subtilis phages from desert soil in the southwest United States and then sequenced and characterized their genomes. Comparative analyses revealed high nucleotide and amino acid similarity between these seven phages, which constitute a novel subcluster. Interestingly, the tail fiber and lysin genes of these phages seem to come from different origins and carry out slightly different functions. These genes were likely acquired by this subcluster of phages via horizontal gene transfer. In conjunction with host range assays, our data suggest that these phages are adapting to hosts with different cell walls.

Mother-Infant Dyadic Synchrony in the NICU Context.

BACKGROUND: Dyadic synchrony is a co-constructed social process relating to the back and forth interactions between mothers and infants that are strongly associated with neurodevelopment, self-regulation, and attachment. In the neonatal intensive care unit (NICU), this process may become interrupted because of the physiological state of the infant, the emotional state of the mother, and the physical environment of the NICU. PURPOSE: In applying Feldman's Biobehavioral Model of Synchrony, this empirical review deconstructs the process of dyadic synchrony in the NICU context and provides a conceptual approach to guide both research and clinical practice. METHODS: First, we examine the theoretical and empirical literature to explicate the primary structural and biophysiological components of synchrony and relate these constructs to the extant research on premature infants. Next, we synthesize the maternal, infant, and contextual factors that facilitate or inhibit the ontogenesis of dyadic synchrony in the NICU. The final section highlights the state of the science in dyadic synchrony in the NICU including gaps and recommendations for future research. FINDINGS: An empirical review synthesis presents a visual conceptual framework to illustrate multiple processes that depict maternal, infant, and contextual influences of mother-infant synchrony in the NICU. IMPLICATIONS FOR PRACTICE/RESEARCH: Despite the challenges posed to mother-infant relationships in the NICU, high-quality mother-infant interactions are possible, dyadic synchrony can emerge, and premature infants can develop secure attachments. Clinicians and researchers can apply this conceptual framework of mother-infant dyadic synchrony in the NICU to promote evidence-based research and clinical practice.

Dietary Exposure of Japanese Quail (Coturnix japonica) to Perfluorooctane Sulfonate (PFOS) and a Legacy Aqueous Film-Forming Foam (AFFF) Containing PFOS: Effects on Reproduction and Chick Survivability and Growth.

Effects of perfluorooctane sulfonate (PFOS) and a legacy aqueous film-forming foam (AFFF) containing 91% PFOS (AFFF PFOS) on reproduction, chick survivability, and growth of Japanese quail (Coturnix japonica) were determined. Day-old Japanese quail were administered PFOS or AFFF PFOS at 6 dietary concentrations ranging from 0 to 21 mg kg-1 feed for a total of 20 wk. At the age of 4 wk, 16 male/female pairs per treatment were assigned to cages, and egg laying was induced by the age of 10 wk. Eggs were collected daily, set weekly, and incubated for 18 d for the following 10 wk. Hatchlings were fed uncontaminated feed for 2 wk and euthanized to collect blood and liver. After 10 wk of egg collection, adults were euthanized to collect blood, liver, and kidneys. Significantly increased myofiber numbers in the liver and glomerular sclerosis in the kidneys of adults indicated damage at greater doses. Perfluorooctane sulfonate or AFFF PFOS did not significantly affect egg production; however, hatchability was decreased at the highest PFOS dose. The no-observed-adverse-effect levels for chick survivability, considered the critical effect, were 4.1 mg PFOS kg feed-1 (0.55 mg kg body wt-1 d-1 ) and 5.0 mg AFFF PFOS kg feed-1 (0.66 mg kg body wt-1 d-1 ), resulting in calculated average toxicity reference values of 0.25 mg kg feed-1 and 0.034 mg kg body weight-1 d-1 . Environ Toxicol Chem 2021;40:2521-2537. © 2020 SETAC.

The Subacute Toxicity of Perfluorooctane Sulfonate and/or Perfluorooctanoic Acid and Legacy Aqueous Film-Forming Foams to Japanese Quail (Coturnix japonica) Chicks.

As part of an effort to develop avian ecotoxicity information for poly- and perfluoroalkyl substances (PFAS) including perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) associated with aqueous film-forming foam (AFFF) used by the Department of Defense, the subacute toxicity of PFOS, PFOA, PFOS + PFOA, 3M AFFF, and Ansul AFFF to Japanese quail (Coturnix japonica) chicks was determined. Ten-day-old Japanese quail were administered treated feed for 5 d and then fed untreated feed for 18 d. Analyzed concentrations of PFOS, PFOA, and PFOS + PFOA ranged from 62 to 1955, 162 to 1208, and 43 + 45 to 296 + 292 mg kg feed-1 . Analyzed concentrations of PFOS in feed containing the 3M AFFF ranged from 73 to 1399 mg kg feed-1 , and formulated concentrations of 6:2 fluorotelomer thioamido sulfonate in feed containing the Ansul AFFF ranged from 9 to 1118 mg kg feed-1 . Average daily doses resulting in 50% mortality at day 5 were 38 (34-43), 68 (63-74), 55 (51-59), and 130 (103-164) mg PFOS, PFOA, PFOS + PFOA, and PFOS in 3M AFFF kg body weight-1 d-1 . Ansul AFFF did not result in any mortalities. Dietary concentrations resulting in 50% mortality at day 5 were 351 (275-450), 496 (427-575), 398 (339-468), and 467 (390-559) mg PFOS, PFOA, PFOS + PFOA, and PFOS in 3M AFFF kg feed-1 . Environ Toxicol Chem 2021;40:695-710. © 2020 SETAC.

Flushing of Stagnant Premise Water Systems after the COVID-19 Shutdown Can Reduce Infection Risk by Legionella and Mycobacterium spp.

There is concern about potential exposure to opportunistic pathogens when reopening buildings closed due to the COVID-19 pandemic. In this study, water samples were collected before, during, and after flushing showers in five unoccupied (i.e., for ∼2 months) university buildings with quantification of opportunists via a cultivation-based assay (Legionella pneumophila only) and quantitative PCR. L. pneumophila were not detected by either method; Legionella spp., nontuberculous mycobacteria (NTM), and Mycobacterium avium complex (MAC), however, were widespread. Using quantitative microbial risk assessment (QMRA), the estimated risks of illness from exposure to L. pneumophila and MAC via showering were generally low (i.e., less than a 10-7 daily risk threshold), with the exception of systemic infection risk from MAC exposure in some buildings. Flushing rapidly restored the total chlorine (as chloramine) residual and decreased bacterial gene targets to building inlet concentrations within 30 min. During the postflush stagnation period, the residual chlorine dissipated within a few days and bacteria rebounded, approaching preflush concentrations after 6-7 days. These results suggest that flushing can quickly improve water quality in unoccupied buildings, but the improvement may only last a few days.

Self-directed action affects planning in tool-use tasks with toddlers.

Toddlers grasp a tool more effectively when it is self-directed (e.g., spoon) than other-directed (e.g., hammer), possibly because the consequences of self-directed actions are more obvious. When the negative consequences of an inefficient grip were made equally salient, the self-directed versus other-directed differences remained.

Fatty acid profile in milk from goats, Capra aegagrus hircus, exposed to perchlorate and its relationship with perchlorate residues in human milk.

Polyunsaturated fatty acids (PUFA) in milk are vital for normal growth and development of infant mammals. Changes in fatty acid composition were observed in milk fat from goats dosed with perchlorate (0.1 and 1 mg/kg body weight/day) for 31 days, but the effect was not persistent. Adaptation may be induced in these goats to compensate for the perchlorate effect. In an analysis of fatty acid composition in human milk samples, a weak negative correlation was observed between perchlorate concentrations and total PUFA in 38 human milk samples.

Evidence of motor planning in infant reaching behavior.

When adults reach for an object, kinematic measures of their approach movement are affected by what they intend to do after grasping it. We examined whether such future intended actions would be reflected in the approach-to-grasp phase of infant reaching. Twenty-one 10-month-old infants were encouraged to either throw a ball into a tub or fit it down a tube. Kinematic measures of the approach phase of the reach toward the ball were obtained using a motion analysis system. Infants, like adults, reached for the ball faster if they were going to subsequently throw it as opposed to using it in the precision action. The perceptual aspects of the ball were the same and cannot account for these kinematic differences. Infants appear to be planning both segments of their actions in advance. Our findings provide evidence for a level of sophistication in infant motor planning not reported before.