Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Anal Chem ; 73(21): 5334-8, 2001 Nov 01.
Article in English | MEDLINE | ID: mdl-11721938

ABSTRACT

Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. The E. coli were labeled with a membrane-permeable nucleic acid stain (Syto15), a membrane-impermeable nucleic acid stain (propidium iodide), or a fluorescein-labeled antibody and counted at rates from 30 to 85 Hz. The observed labeling efficiencies for the dyes and antibody were greater than 94%.


Subject(s)
Escherichia coli/metabolism , Flow Cytometry/instrumentation , Flow Cytometry/methods , Cell Membrane Permeability , Electrophoresis/methods , Escherichia coli/cytology , Escherichia coli/genetics , Fluorescent Antibody Technique/methods , Fluorescent Dyes/metabolism , Osmosis
SELECTION OF CITATIONS
SEARCH DETAIL
...