Mapping Sleep Spindle Characteristics to Vigilance Outcomes in Patients with Obstructive Sleep Apnea.

Obstructive Sleep Apnea (OSA) is a sleep disorder associated with reduced vigilance. Vigilance status is often measured using the Psychomotor Vigilance Task (PVT). This paper investigates modelling strategies to map sleep spindle (Sp) characteristics to PVT metrics in patients with OSA. Sleep spindles (n=2305) were manually detected across blocks of sleep for 20 patients randomly selected from a cohort of 190 undergoing Polysomnography (PSG) for suspected OSA. Novel Sp metrics based on runs or "bursts" of Sps were used to model Sp characteristics to standardized (z) Lapse and Median Reaction Time (MdRT) scores, and to Groups based on zLapse and zMdRT scores. A model employing Sp Burst characteristics mapped to MdRT Group membership with an accuracy of 91.9%, (95% C.I. 90.8-93.0). The model had a sensitivity of 88.9%, (95% C.I. 87.5-89.0) and specificity of 89.1% (95% C.I. 87.3-90.5) for detecting patients with the lowest MdRTs in our cohort.Clinical Relevance- Based on these results it may be possible to use Sp data collected during overnight diagnostic PSG for OSA to detect patients at risk for attention deficits. This would improve triage for OSA therapy by identifying at risk patients at the time of OSA diagnosis and would remove the need to employ additional testing to assess vigilance status.

Pre-exercise screening and health coaching in CHD secondary prevention: a qualitative study of the patient experience.

Secondary prevention programmes can be effective in reducing morbidity and mortality from coronary heart disease (CHD). In particular, UK guidelines, including those from the Department of Health, emphasize physical activity. However, the effects of secondary prevention programmes with an exercise component are moderate and uptake is highly variable. In order to explore patients' experiences of a pre-exercise screening and health coaching programme (involving one-to-one consultations to support exercise behaviour change), semi-structured telephone interviews were undertaken with 84 CHD patients recruited from primary care. The interviews focused on patients' experiences of the intervention including referral and any recommendations for improvement. A thematic analysis of transcribed interviews showed that the majority of patients were positive about referral. However, patients also identified a number of barriers to attending and completing the programme, including a belief they were sufficiently active already, the existence of other health problems, feeling unsupported in community-based exercise classes and competing demands. Our findings highlight important issues around the choice of an appropriate point of intervention for programmes of this kind as well as the importance of appropriate patient selection, suggesting that the effectiveness of health coaching may be under-reported as a result of including patients who are not yet ready to change their behaviours.

Effects of injectate volume on thermodilution measurements of cardiac output in patients with low ventricular ejection fraction.

OBJECTIVE: To determine the effect of 5-mL injectate on cardiac output measurements in critically ill patients with low ventricular ejection fraction (< 35%). METHODS: Thermodilution cardiac output measurements obtained with three 5-mL and three 10-mL (randomly ordered) iced injectates in 50 patients with low ejection fraction were averaged if the measurements were within 10% of the median. If the 3 measurements were not within those limits, additional measurements were obtained. RESULTS: Cardiac output measured with the 5-mL injectate (mean, 4.63 L/min) and cardiac output measured with the 10-mL injectate (mean, 4.52 L/min) were not significantly different (P = .64). Lower and upper limits of agreement were -1.7 L/min to +1.6 L/min. The bias (mean difference between 10- and 5-mL measurements) of all measurements was -0.09, and the precision was 1.43 L/min, with a 95% confidence limit (mean difference +/- 2 SD) of -1.7 to +1.6 L/min. An additional measurement was necessary in 77% of patients in the 5-mL group but in only 48% of the 10-mL group (P = .006). CONCLUSIONS: Cardiac outputs measured with 5- and 10-mL injectates do not differ significantly. The greater variability of measurements obtained with a 5-mL injectate suggests that more measurements, and thus more time, are needed to measure cardiac output accurately. Clinicians must weigh the benefit of minimizing fluid volume used against the potential decreased reliability of cardiac output measurements.

Structure-based sequence alignment of three AdoMet-dependent DNA methyltransferases.

M.HhaI, M.TaqI and COMT are DNA methyltransferases (MTases) which catalyze the transfer of a methyl group from the cofactor AdoMet to C5 of cytosine, to N6 of adenine and to a hydroxyl group of catechol, respectively. The larger catalytic domains of the bilobal proteins, M.HhaI and M.TaqI, and the entire single domain of COMT have an alpha/beta structure containing a mixed central beta-sheet. These domains have very similar folding. By allowing appropriate 'insertions' or 'deletions' in the backbones of the three structures, it was possible to find more conserved motifs in M.TaqI and COMT. The similarity in protein folding and the equivalence of amino-acid sequences revealed by the structural alignment indicate that many AdoMet-dependent MTases may share a common catalytic domain structure.