Effects of roughage inclusion and particle size on digestion and ruminal fermentation characteristics of beef steers.

Roughage is fed in finishing diets to promote ruminal health and decrease digestive upset, but the inclusion rate is limited because of the cost per unit of energy and feed management issues. Rumination behavior of cattle may be a means to standardize roughage in beef cattle finishing diets, and increasing the particle size of roughage could modulate the ruminal environment and aid in maintaining ruminal pH. Therefore, this experiment was conducted to determine the effects of corn stalk (CS) inclusion rate and particle size in finishing diets on digestibility, rumination, and ruminal fermentation characteristics of beef steers. Four ruminally cannulated steers were used in a 4 × 4 Latin square experiment. Treatments were arranged as a 2 × 2 factorial with treatments consisting of 5% inclusion of a short-grind roughage (5SG), 10% inclusion of a short-grind roughage (10SG), 5% inclusion of a long-grind roughage (5LG), and 10% inclusion of a long-grind roughage (10LG). Differences in particle size were obtained by grinding corn stalks once (LG) or twice (SG) using a commercial tub grinder equipped with a 7.6-cm screen and quantified using the Penn State Particle Separator (PSPS) to estimate physically effective NDF (peNDF). Each period included 14 d for adaptation and 4 d for diet, fecal, and ruminal fluid collections. Animals were outfitted with rumination monitoring collars to continuously measure rumination activity. The 10LG treatment had a greater ( < 0.01) percentage of large particles (retained on the top 3 sieves of the PSPS) compared to the other treatments. This resulted in a greater ( < 0.01) percentage of estimated peNDF for the 10LG diet compared to the others. Feeding diets containing 5% roughage tended to increase ( ≤ 0.09) DM, NDF, and starch total tract digestibility compared to diets containing 10% roughage. Cattle consuming LG treatments had greater ( < 0.01) rumination time and greater ( < 0.01) ruminal pH than cattle consuming diets containing SG roughage. Cattle receiving the 5% inclusion rate of roughage tended to have greater ( = 0.09) time (h/d) under a ruminal pH of 5.6 and a larger ( = 0.03) area under the threshold compared to cattle receiving the 10% roughage treatments. Overall, feeding a lower inclusion of roughage with a larger particle size may stimulate rumination and aid in ruminal buffering similar to that of a higher inclusion of roughage with a smaller particle size, without negatively impacting digestibility and fermentation.

Effects of feeding condensed distiller's solubles and crude glycerin alone or in combination on finishing beef cattle performance, carcass characteristics, and in vitro fermentation.

Two experiments were conducted to evaluate the effects of feeding condensed distiller's solubles (DS) and crude glycerin alone or in combination on performance of finishing beef cattle and in vitro fermentation. In both experiments, dietary treatments consisted of a steam-flaked corn-based diet with 0% DS or crude glycerin (CON), 10% DS (CDS), 10% crude glycerin (GLY), or a combination of 5% DS and 5% crude glycerin (C+G) included on a DM basis. All treatment diets contained 15% (DM basis) wet distiller's grains plus solubles. In Exp. 1, crossbred steers ( = 256; 322 ± 15 kg initial BW) were used in a randomized complete block finishing trial. Growth performance and gain efficiency were not different ( > 0.10) across all treatments. Treatment had no effect ( > 0.10) on carcass weight, marbling score, yield grade, LM area, or percent grading USDA choice. In Exp. 2, ruminal fluid was collected from 2 ruminally cannulated steers to evaluate in vitro fermentation characteristics. No differences ( = 0.43) were observed for DM disappearance across all treatments. The GLY and C+G treatments had decreased ( = 0.02 and = 0.05, respectively) NDF disappearance (NDFD), whereas the CDS treatment tended to have decreased ( = 0.06) NDFD compared with CON. Concentrations of NH decreased ( < 0.04) with GLY and C+G treatments compared with CON. Total gas production decreased ( < 0.01) for the C+G treatment compared with other treatments. The CDS treatment had increased ( < 0.02) total VFA compared with the CON or C+G treatments. Treatments had no effect ( > 0.17) on concentrations of acetate, propionate, and butyrate, but valerate concentrations were the greatest ( < 0.04) for the CON treatment. Concentrations of isobutyrate increased ( < 0.05) for the CON and C+G treatments compared with the GLY treatment and tended to increase ( < 0.10) compared with the CDS treatment. Including DS or glycerin in the substrates decreased ( < 0.08) isovalerate compared with CON. Feeding additional byproducts such as DS and crude glycerin alone or in combination in a finishing diet did not alter live animal performance or carcass characteristics; however, feeding a combination of the 2 byproducts may alter in vitro fermentation.

Effects of roughage inclusion and particle size on performance and rumination behavior of finishing beef steers.

Roughage is typically mechanically processed to increase digestibility and improve handling and mixing characteristics in beef cattle finishing diets. Roughage is fed to promote ruminal health and decrease digestive upset, but inclusion in finishing diets is limited due to the cost per unit of energy. Rumination behavior may be a means to standardize roughage in beef cattle finishing diets, and increasing particle size of roughage may allow a decrease in roughage inclusion without sacrificing animal performance. Therefore, the objectives of this study were to quantify rumination time for a finishing beef animal and to evaluate the effects of corn stalk (CS) inclusion rate and particle size on rumination behavior, animal performance, and carcass characteristics. Fifty-one individually fed steers (385 ± 3.6 kg initial BW) were used in a randomized complete block design feeding study. Corn stalks were passed through a tub grinder equipped with a 7.62-cm screen once to generate long-grind CS (LG-CS) or twice to generate short-grind CS (SG-CS). Dietary treatments were based on steam-flaked corn and included, on a DM basis, 30% wet corn gluten feed (WCGF) with 5% SG-CS (5SG), 30% WCGF with 5% LG-CS (5LG), and 25% WCGF with 10% SG-CS (10SG). The Penn State Particle Separator was used to separate ingredients and treatment diets and to estimate physically effective NDF (peNDF). On d 70, each steer was fitted with a collar (HR Tag; SCR Dairy, Netanya, Isreal), which continuously measured rumination minutes via a sensory microphone. Long-grind CS contained more ( < 0.01) peNDF than SG-CS, and the 10SG diet contained more ( = 0.03) peNDF than the 5LG and 5SG diets. Dry matter intake was greatest ( = 0.03) for steers consuming 5LG and least for steers consuming 10SG, with cattle consuming 5SG being intermediate. Carcass-adjusted ADG and G:F were greatest ( ≤ 0.03) for steers consuming 5LG and 5SG compared with steers consuming 10SG. Hot carcass weight tended ( = 0.10) to be greatest for steers consuming 5LG and least for steers consuming 10SG, with steers consuming 5SG being intermediate. Dressing percent was greater ( = 0.01) for steers consuming 5LG and 5SG than for steers consuming 10SG. A significant interaction ( < 0.01) occurred for rumination minutes × day. Rumination (min/day) were greatest ( = 0.01) for steers consuming 10SG followed by steers consuming 5LG and was lowest for steers consuming 5SG. Increasing particle size of roughage may be a means to decrease roughage inclusion rate while maintaining rumination and performance.

Effects of glycerin concentration in steam-flaked corn-based diets with supplemental yellow grease on performance and carcass characteristics of finishing beef steers.

Forty-eight individually fed crossbred steers (initial BW = 381 ± 7.61 kg) were used to determine the effects of glycerin (GLY) concentration in steam-flaked corn (SFC)-based diets with added yellow grease on animal performance and carcass characteristics. Glycerin was included at 0, 2.5, 5, and 10% dietary DM replacing SFC. A completely randomized design was used with steers as the experimental unit, and the model included the fixed effects of diet. Contrast statements were used to determine linear and quadratic effects of GLY inclusion. Final BW and DMI were not affected (P > 0.27) by GLY concentration. Average daily gain and G:F based on live BW did not differ as GLY level increased in the diet from 0 to 10% of DM (P > 0.33). Carcass-adjusted final BW and carcass-adjusted G:F were also not affected by GLY concentration (P > 0.22); however, carcass-adjusted ADG tended to respond quadratically by decreasing from 2.5 to 5% GLY inclusion and increasing thereafter (P = 0.10). Calculated dietary NEm and NEg did not differ as GLY increased in the diet (P > 0.37). Hot carcass weight tended to respond quadratically, decreasing from 2.5 to 5% GLY and increasing thereafter (P = 0.10). Likewise, dressing percentage tended to respond quadratically by decreasing from 2.5 to 5% GLY inclusion and increasing to 10% GLY inclusion (P = 0.09). Fat thickness decreased linearly as GLY inclusion increased in the diet (P < 0.01); however, no differences were detected in marbling score and LM area (P > 0.21). Calculated yield grade (YG) decreased linearly as GLY increased in the diet from 0 to 10% of DM (P = 0.04). Based on our results, there was no animal performance benefit for replacing SFC with GLY in diets containing yellow grease, and the only change in carcass merit was a slight improvement in YG.

Effects of 35% corn wet distillers grains plus solubles in steam-flaked and dry-rolled corn-based finishing diets on animal performance, carcass characteristics, beef fatty acid composition, and sensory attributes.

Fifty-four individually-fed Hereford-Angus cross steers (initial BW = 308 ± 9 kg) were used in an unbalanced randomized block design with a 2 × 2 factorial treatment arrangement to determine effects of corn processing method and corn wet distillers grains plus solubles (WDGS) inclusion in finishing diets on animal performance, carcass and beef characteristics, and sensory attributes. Dietary treatments included steam-flaked corn- (SFC) and dry-rolled corn (DRC)-based finishing diets containing 0 or 35% WDGS (DM basis; 0SFC and 35SFC, 0DRC and 35DRC, respectively). Yellow grease was used to equilibrate fat content of diets. Steers were fed 174 d, and were harvested on a single date when the mean ultrasound fat thickness was estimated to be 1.30 cm. No interactions between corn processing and WDGS were observed for performance or carcass characteristics (P ≥ 0.11). Final BW (556 ± 14 kg) and ADG (1.43 ± 0.06 kg) were not affected (P ≥ 0.25) by dietary treatment. Steers fed SFC-based diets consumed less feed, and were 10.6% more efficient (P < 0.01) than those fed DRC-based diets. Including WDGS in finishing diets improved feed efficiency of steers consuming both SFC- and DRC-based diets (P ≤ 0.04). Dietary treatment did not affect HCW, dressing percentage, fat thickness, or yield grade (P ≥ 0.27). Including WDGS in finishing diets decreased the concentration of 16:1cis-9, 18:1cis-9, and 18:1cis-11 fatty acids, and tended (P ≤ 0.10) to increase total fat concentration of steaks compared with diets without WDGS. A corn processing method by WDGS interaction was detected for 18:1trans-11 where steaks from 0DRC diets had decreased concentrations compared with other diets. There were no dietary effects on palatability attributes (P > 0.20). Livery-organy aromatics (P = 0.03) and sweet basic tastes (P = 0.01) in steaks from the 35SFC treatment were more intense than in other treatments, but were barely detectable. Thiobarbituric acid reactive substances tended to be greater in steaks from steers fed WDGS after 5 d of storage (P = 0.10), and were greater after 7 d. (P < 0.01). Inclusion of WDGS used in this experiment improved G:F with minimal impacts on carcass characteristics. Both WDGS inclusion and corn processing method impacted fatty acid composition. However, diet had minimal impacts on palatability attributes. When compared with diets fat-equilibrated with yellow grease, the primary concern with incorporating WDGS appears to be decreased shelf-life after 5 d of storage.

Effects of twenty percent corn wet distillers grains plus solubles in steam-flaked and dry-rolled corn-based finishing diets on heifer performance, carcass characteristics, and manure characteristics.

Two hundred sixty-four crossbred heifers (initial BW = 354 kg ± 0.5) were used to determine effects of corn processing method and wet distillers grains plus solubles (WDGS) inclusion in finishing diets on animal performance, carcass characteristics, and manure characteristics. The study was conducted as a randomized complete block with a 2 × 2 factorial arrangement of treatments. Dietary treatments included steam-flaked corn (SFC)- and dry-rolled corn (DRC)-based finishing diets containing 0 or 20% WDGS (0SFC, 20SFC, 0DRC, and 20DRC, respectively). Heifers averaged 154 d on feed and were marketed in 3 groups. There were no interactions between corn processing method and WDGS detected (P ≥ 0.29) for any performance or carcass response variables. Heifers fed diets containing WDGS tended to have greater final BW (P = 0.10) and increased G:F (P = 0.08) compared with heifers fed diets without WDGS. Heifers fed SFC-based diets consumed 7% less feed (P < 0.01) and were 9% more efficient (P < 0.01) than heifers fed DRC-based diets. Carcass characteristics were not affected by corn processing method or WDGS inclusion (P ≥ 0.16). Intakes of OM, N, P, and K were greater (P ≤ 0.05) for heifers fed DRC-based diets than those fed SFC-based diets, which resulted in greater net accumulation of the nutrients in the manure (P ≤ 0.04). Heifers fed diets containing WDGS had greater (P < 0.01) intakes of N, P, and K than heifers fed diets without WDGS. As a result, a greater net accumulation of P and K (P ≤ 0.03) and N (P = 0.10) were present in the manure from cattle fed diets containing WDGS compared with those fed diets without WDGS. There was no interaction (P ≥ 0.16) between corn processing and WDGS on N volatilization losses. Nitrogen volatilization losses from manure (expressed as a percentage of intake and g·heifer(-1)·d(-1)) were greater (P < 0.01) for heifers fed SFC-based diets than heifers fed DRC-based diets. Feeding DRC-based finishing diets to heifers resulted in increased manure production and nutrient excretion and decreased N volatilization. Both corn processing method and WDGS inclusion affected animal performance and manure characteristics.

Use of dried distillers grains throughout a beef production system: effects on stocker and finishing performance, carcass characteristics, and fatty acid composition of beef.

A 2-yr study was conducted using a 3 × 2 factorial arrangement of treatments to evaluate the effects of feeding dried distillers grains throughout a beef production system on performance, carcass characteristics, and fatty acid profile of beef. Factors were wheat pasture supplement [no supplement (CON), dry-rolled corn (DRC), and dried distillers grains (DDG)] fed at 0.5% BW daily and finishing diet [steam-flaked corn based diet containing 0 (SFC) or 35% (35DDG) DDG]. Each year, 60 preconditioned Hereford steers (initial BW = 198 kg ± 3) grazed winter wheat pasture with or without supplement. Body weight gain was 8% greater for steers consuming DDG supplement compared with CON and DRC steers (P < 0.01). After the grazing period, pastures within supplement treatment were randomly assigned to SFC or 35DDG. There was no supplement by finishing diet interaction for any performance or carcass variable of interest (P ≥ 0.41). Previous supplementation on winter wheat affected BW at feedlot entry and adjusted G:F (P ≤ 0.05) but had no effect on finishing ADG or carcass traits (P ≥ 0.12). On a carcass-adjusted basis, steers consuming 35DDG had reduced final BW, ADG, G:F, and total BW gain throughout the system (P ≤ 0.04) compared with SFC. Additionally, steers consuming 35DDG had reduced HCW, dressing percent, and fat thickness (P ≤ 0.03) compared with SFC. There was a supplement by finishing diet interaction (P = 0.02) for 18:0, in which cattle supplemented with DRC and fed the SFC finishing diet had the lowest concentration of 18:0 but DRC supplemented steers fed the 35DDG diet had the greatest concentration. The interaction was not significant (P ≥ 0.18) for other fatty acids. Main effects of supplement and finishing diet affected (P ≤ 0.05) several other fatty acids of interest, particularly 18:2, which is associated with reduced flavor-stability of beef. The use of DDG as a supplement to wheat pasture resulted in greater ADG during wheat grazing and heavier BW at feedlot entry, but final BW was not different from CON or DRC groups. Feeding DDG at 35% DM in steam-flaked corn-based finishing diets reduced ADG, G:F, and HCW, and affected the fatty acid composition of beef.

Wet distillers grains plus solubles concentration in steam-flaked-corn-based diets: Effects on feedlot cattle performance, carcass characteristics, nutrient digestibility, and ruminal fermentation characteristics.

Two experiments were conducted to determine the effects of wet distillers grain plus solubles (WDG; <15% sorghum grain) concentration in steam-flaked corn (SFC) diets on feedlot performance, carcass characteristics, ruminal fermentation, and diet digestibility. In Exp. 1, six hundred crossbred steers (364 ± 35 kg of BW) were used in a randomized complete block design with 8 replications/treatment. Dietary treatments consisted of a dry-rolled corn (DRC) control diet without WDG, a SFC control without WDG, and SFC with 4 WDG concentrations (15, 30, 45, 60% DM basis) replacing SFC, cottonseed meal, urea, and yellow grease. Final BW, ADG, G:F, HCW, and 12th-rib fat depth were greater (P ≤ 0.05) for SFC compared with DRC. Dry matter intake tended (P = 0.06) to be greater for DRC compared with SFC. Final BW, ADG, G:F, HCW, 12th-rib fat depth, and marbling score decreased linearly (P < 0.01) with increasing WDG concentration. In Exp. 2, six ruminally and duodenally cannulated crossbred steers (481 ± 18 kg of BW) were used in a 6 × 6 Latin square design using the same diets as Exp. 1. Ruminal, postruminal, and total tract OM and NDF digestibility were not different (P > 0.14) for DRC compared with SFC. Ruminal and total tract starch digestibility were greater (P < 0.01) for SFC compared with DRC. Dry matter and OM intake were not different (P ≥ 0.43) among WDG treatments. Ruminal and total tract OM digestibility decreased linearly (P < 0.01) with increasing WDG concentration. Intake, ruminal digestibility, and total tract digestibility of NDF increased linearly (P < 0.01) with increasing WDG concentration. Starch intake decreased linearly (P < 0.01) with increasing WDG concentration. Ruminal starch digestibility increased (P = 0.01) with increasing concentration of WDG. Total tract starch digestibility decreased quadratically (P < 0.01) with increasing concentration of WDG. Feeding SFC improved steer performance compared with DRC. The concentration of WDG and corn processing method influences nutrient digestibility and ruminal fermentation. The addition of WDG in SFC-based diets appears to negatively affect animal performance by diluting the energy density of the diet.

Effects of different growing diets on performance, carcass characteristics, insulin sensitivity, and accretion of intramuscular and subcutaneous adipose tissue of feedlot cattle.

Forty-eight individually fed crossbred steers (British and British x Continental; BW=296+/-16.7 kg) were used to evaluate effects of different growing diets on changes in accretion of intramuscular (IMF) and subcutaneous (SCF) adipose tissues, insulin sensitivity, and carcass traits. Dietary treatments were AL-LC (a low-corn diet fed to allow cattle ad libitum access to feed), AL-HC (a high-corn diet fed to allow cattle ad libitum access to feed), LF-HC (a limit fed high-corn diet with the energy intake equal to that provided by AL-LC), and AL-IC (a diet with approximately the midpoint daily energy intake between AL-LC and AL-HC). Steers received treatments until d 56, after which all groups were fed AL-HC until d 140. Real-time ultrasound and BW measurements were taken every 28 d, and 3 glucose tolerance tests (GTT) were conducted on d 0, 28, and 56 of the growing period to assess insulin sensitivity. Based on ultrasound IMF and SCF readings during the growing phase, AL-HC and AL-IC increased accretion of IMF (P=0.01), and AL-LC and LF-HC diets resulted in less accretion of SCF (P<0.01) compared with other treatments. During the finishing period, accretion of IMF (P=0.13) and SCF (P=0.81) did not differ among treatments, which diluted differences in overall (d 0 to 140) accretion of IMF (P=0.28) and SCF (P=0.52), such that final real-time ultrasound measures of IMF and SCF did not differ (P >or= 0.36) among treatments. Actual carcass marbling scores, however, were greater for the AL-HC and AL-IC treatments (P=0.02), and 12th-rib fat thickness tended (P=0.08) to be greater for AL-HC and AL-IC groups. Based on incremental area under the curve and area over the curve as indicators of insulin release and glucose uptake, respectively, no differences (P >or= 0.10) in insulin sensitivity were observed among treatments. Our results suggest that high-corn diets increase growing phase accretion of IMF and SCF; however, these differences were not related to differences in glucose and insulin kinetics.

Effects of phase feeding of protein on performance, blood urea nitrogen concentration, manure nitrogen:phosphorus ratio, and carcass characteristics of feedlot cattle.

Two experiments with a randomized complete block design were conducted to determine the effects of phase feeding of CP on performance, blood urea nitrogen (BUN), manure N:P ratio, and carcass characteristics of steers fed in a feedlot. In Exp. 1, 45 crossbred steers (initial BW = 423 +/- 3.3 kg) were individually fed a diet formulated to contain 13.0% CP (DM basis) for 62 d. On d 63, the dietary CP was maintained at 13.0% or formulated to contain 11.5 or 10.0% CP until slaughter. Actual CP values were 12.8, 11.8, and 9.9%, respectively. Reducing the CP concentration of the diet did not affect ADG of steers from d 62 to 109 (P = 0.54) or over the 109-d feeding period (1.45, 1.50, and 1.49 kg/d for 13.0, 11.5, and 10.0% CP, respectively; P = 0.85). No differences (P > 0.12) among treatments were detected for BUN concentrations on d 0, 62, or 109. Gain:feed, DMI, and carcass characteristics did not differ among treatments (P > 0.10). In Exp. 2, 2 trials were conducted using 184 (initial BW = 406 +/- 2.6 kg) and 162 (initial BW = 342 +/- 1.9 kg) crossbred steers. Data from the 2 trials were pooled for statistical analysis, and trial effect was added to the statistical model. Steers were fed a diet formulated to contain 13.0% CP until reaching approximately 477 kg. When the average BW of the pen was 477 kg, diets were maintained at 13.0% CP or reduced to contain 11.5 or 10.0% CP. Actual CP values were 12.4, 11.5, and 9.3% CP for treatments 13.0, 11.5, and 10.0% CP, respectively. Reducing the CP content of the diet did not affect ADG after the diet changed (P = 0.16) or throughout the finishing period (P = 0.14). Immediately before slaughter, steers fed the 13.0% CP diet had greater (P < 0.001) BUN concentrations than steers fed the 11.5 and 10.0% CP diets. Carcasses from cattle fed the 11.5% CP diet had greater (P = 0.02) fat thickness than the 13.0 and 10.0% CP treatments, whereas carcasses from cattle fed 13.0% CP had greater (P = 0.004) marbling scores than steers fed the 11.5 or 10.0% CP diets. Other carcass characteristics, DMI, and G:F did not differ (P > 0.10) among treatments. The N:P ratio was increased with the 10.0% CP diet (P = 0.02) compared with the 11.5 or 13.5% CP treatments; however, manure composition did not differ (P > 0.10) among treatments. These results indicate that reduced CP concentration during the finishing period does not affect feedlot performance but can improve the N and P relationship in the manure.

Influence of oscillating dietary crude protein concentration on performance, acid-base balance, and nitrogen excretion of steers.

Decreasing dietary N inputs into beef cattle feeding operations could potentially decrease environmental concerns relating to air and water quality. Previous studies with sheep suggest that oscillating dietary CP concentrations may improve N use efficiency and thereby decrease dietary N requirements. Therefore, two studies were conducted to determine the effects of oscillating dietary CP concentrations on performance, acid-base balance, and manure characteristics of steers fed high-concentrate diets. Steers were fed to a constant backfat thickness in both studies. In the first trial, 92 steers (mean BW = 408 +/- 2.8 kg; four pens/treatment) were fed the following diets: 1) constant 12% CP, 2) constant 14% CP, and 3) 10 and 14% CP oscillated at 2-d intervals. Steer performance and carcass characteristics were measured. In the second trial, 27 steers were individually fed the same three experimental dietary regimens (nine steers/treatment). Animal performance, arterial acid-base balance, plasma metabolites, and fecal characteristics were measured. In both trials, steers fed the 14% CP diet tended (P < 0.10) to have greater ADG and gain:feed than steers fed the 12% CP diet. Steers fed the oscillating CP regimen had intermediate performance. In Trial 1, steers fed the 14% CP diet tended (P = 0.09) to have smaller longissimus area and higher quality grades than steers fed the oscillating CP regimen. Protein retentions (g/d) calculated from NRC (2000) equations were greater (P = 0.04) for steers fed the 14% CP diet than steers fed the 12% CP diet. Steers fed the oscillating CP regimen tended (P = 0.08) to have greater calculated protein retention (g/d) than steers fed the 12% CP diet. Steers fed the 14% CP diet had greater (P < 0.05) calculated urinary N excretion than steers fed the 12% CP or oscillating CP regimens. Venous plasma concentrations of urea N were greater (P < 0.001) in steers fed the 14% CP diet than in steers fed the 12% CP diet; steers fed the oscillating CP regimen were intermediate but fluctuated over days. Based on arterial blood gas concentrations, acid-base balance was not significantly affected by dietary CP regimen. Results of these trials suggest that the CP requirement of steers in these studies was greater than 12% of the diet DM, and/or that the degradable CP requirement was greater than 6.3% of diet DM. However, the effects of oscillating dietary CP were minimal.

Ranch-management factors associated with antibody seropositivity for Neospora caninum in consignments of beef calves in Texas, USA.

A study was conducted with a 1998 retained-ownership population of Texas (USA) beef calves to determine the ranch-management practices associated with calf seroprevalence to Neospora caninum. Management practices of 76 Texas ranches that consigned 760 calves to a retained-ownership feedlot program were reviewed from a mailed questionnaire. Ninety-nine of 760 (13%; 95% CI, 9.4%, 17.7%) calves were positive to N. caninum and 59% of the ranches consigned at least one positive calf. In the logistic multiple-regression model which controlled for overdispersion, increased odds of calf-level seropositivity was associated with seasonal calving patterns, with stocking>1cow/calfunit/2.2ha, using a round-bale feeder, allowing wildlife access to the weaning supplement, and self-reared replacement heifers. However, decreased odds of seropositivity was associated with using a cattle-working dog and with using a self-contained cattle feeder. There was substantial overdispersion due to ranch.

Association of serologic status for Neospora caninum with postweaning weight gain and carcass measurements in beef calves.

OBJECTIVE: To determine the seroprevalence for Neospora caninum in a population of beef calves in a feedlot and the association of serologic status with postweaning weight gain and carcass measurements. DESIGN: Longitudinal observational study. ANIMALS: 1,009 weaned beef steers from 92 herds. PROCEDURE: Samples were obtained from all steers at time of arrival at a feedlot. Serologic status for Neospora spp was determined, using an agglutination test. Results of serologic testing were compared with calf growth and carcass data, using multivariate regression with generalized estimating equations. RESULTS: Of 1,009 calves, 131 (12.98%) were seropositive, and 54 of 92 (58.7%) consignments had > or = 1 seropositive calf. Median within-consignment prevalence for consignments in which there was > or = 1 seropositive calf was 20%. Seropositive status was associated with significant reductions in average daily gain, live body weight at slaughter, and hot carcass weight and an increase in ribeye area-to-hot carcass weight ratio. Seropositive status also was associated with significant increases in cost of treatment and significant reductions in income. Sick seropositive calves had the highest cost of treatment. An economic loss of $15.62/calf was projected for seropositive calves. CONCLUSIONS AND CLINICAL RELEVANCE: Significant reductions in postweaning weight gain, carcass weight, and economic return were associated with detection of antibodies to N caninum in beef calves in a feedlot.

Association between infection of the respiratory tract attributable to bovine coronavirus and health and growth performance of cattle in feedlots.

OBJECTIVE: To determine the association between respiratory tract infection with bovine coronavirus (BCV), treatment for respiratory tract disease, pulmonary lesions at slaughter, and average daily gain in cattle in feedlots. ANIMALS: 837 calves in feedlots in Ohio and Texas. PROCEDURE: Nasal swab specimens were obtained from cattle at arrival in a feedlot (day 0) and at various times during the initial 28 days after arrival. Specimens were tested for BCV, using an antigen-capture ELISA. Serum samples were obtained at arrival and again 28 days after arrival and tested for antibodies to BCV, using an antibody-detection ELISA. Information was collected regarding treatment for cattle with respiratory tract disease and average daily gain during the feeding period. Pulmonary lesions were evaluated at slaughter. RESULTS: Cattle shedding BCV from the nasal cavity and developing an antibody response against BCV were 1.6 times more likely to require treatment for respiratory tract disease than cattle that did not shed the virus or develop an immune response against BCV. Additionally, cattle that shed BCV from the nasal cavity were 2.2 times more likely to have pulmonary lesions at slaughter than cattle that did not shed the virus. The BCV shedding or seroconversion status did not affect average daily gain. CONCLUSIONS AND CLINICAL RELEVANCE: Bovine coronavirus infects feedlot cattle and is associated with an increased risk for cattle developing respiratory tract disease and pulmonary lesions. Development of appropriate control measures could help reduce the incidence of respiratory tract disease.

Forage intake by and site and extent of digestion in beef cattle grazing midgrass prairie rangeland or plains bluestem pasture throughout the summer.

Eight beef steers fitted with esophageal (four steers/pasture) and 12 beef calves fitted ruminal and duodenal (six calves/pasture; beginning BW = 267 +/- 6 kg) cannulas grazed either midgrass prairie rangeland (excellent range condition; MIDGRASS) or plains bluestem (Bothriochloa ischaemum var. Plains) pasture (BLUESTEM) during mid-May, late June, mid-August, and mid-October of 1990 and 1991 in order to compare nutrient intake and digestion. Forage OM intake (OMI) by cattle grazing MIDGRASS or BLUESTEM was similar (P > .05) in June and August. In May and October, cattle grazing MIDGRASS consumed more (P < .05) OM than cattle grazing BLUESTEM. The extent of true ruminal OM digestion was similar (P > .05) between forage types except in October 1991, when the extent of digestion for BLUESTEM was greater (P < .05) than for MIDGRASS. The N intake by cattle interacted by year and forage (P < .05). Nitrogen intake by cattle grazing MIDGRASS tended to be lower in June and August than in May and October. The N intake by cattle grazing BLUESTEM peaked (P < .05) in August during 1990; however, N intake was lowest (P < .05) in August during 1991. Duodenal non-ammonia N (NAN) flow was higher (P < .05) in cattle grazing BLUESTEM than in cattle grazing MIDGRASS from May through August; however, duodenal NAN flow in cattle grazing BLUESTEM was lower (P < .05) in October 1991. Duodenal microbial N synthesis (grams/day) responded quadratically (P < .05) to total ruminal OM digestion (kilograms/day). Extent of true ruminal N digestion of both forages decreased (P < .05) as forage became more mature and lower in total N. Midgrass prairie seemed superior to BLUESTEM in May and October because of higher energy intakes and BLUESTEM seemed to be a good alternative to MIDGRASS during June through August, suggesting that these forages would make excellent complements. Furthermore, these data suggest that, in cattle grazing either forage, duodenal NAN flow was disproportionately high relative to energy intake.

Diet quality and ruminal digestion in beef cattle grazing midgrass prairie rangeland or plains bluestem pasture throughout the summer.

Beef cattle fitted with esophageal (four steers/pasture) or ruminal and duodenal (six calves/pasture; beginning BW +/- SE = 267 +/- 6 kg) cannulas grazed midgrass prairie rangeland (excellent range condition; MIDGRASS) or plains bluestem (Bothriochloa ischaemum var. Plains) pasture (BLUESTEM) in mid-May, late-June, mid-August, and mid-October of 1990 and 1991. Nitrogen in masticate samples collected from MIDGRASS was lowest (P < .05) in June and August across both years. The N in BLUESTEM masticate peaked (P < .05) in August 1990, but N was lowest (P < .05) in August 1991. The detergent fiber content of masticate from both forages increased (P < .05) as the grazing season advanced from May through August; fall regrowth in October occasionally resulted in a small decrease (P < .05) in fiber content. In vitro OM disappearance (IVOMD) followed a pattern similar to N content. The IVOMD of BLUESTEM masticate was greater (P < .05) than that of MIDGRASS masticate. The ruminal ammonia N concentration (milligrams/deciliter) in cattle grazing BLUESTEM (4.5) usually was greater (P < .05) than in cattle grazing MIDGRASS (3.3). In situ OM and N disappearance was greater (P < .05) from BLUESTEM masticate than from MIDGRASS masticate in May, June, and August. The ruminally degraded N:ruminally degraded OM ratio (grams/kilograms) estimated from in situ digestion suggested that cattle grazing MIDGRASS during the mid-summer of both years and BLUESTEM in August 1991 may have been marginally deficient in ruminally degraded N. Plains bluestem pasture would complement MIDGRASS by providing better quality grazing during the mid-summer.

Influence of high-starch vs high-fiber energy supplements on performance of stocker cattle grazing wheat pasture and subsequent feedlot performance.

A 3-yr study was conducted to determine effects of high-starch (HS) or high-fiber (HF) energy supplements on performance of fall-weaned steer calves (n = 192, Exp. 1 and 2; n = 84, Exp. 3) grazing winter wheat pasture (Triticum aestivum variety 2157) and subsequent feedlot performance. The steers received 1) no supplement (CL) other than free-choice access to a commercial mineral mixture or 2) were hand-fed 6 d/wk either a corn-based HS supplement, or 3) a soybean hull/wheat middling-based HF supplement. In Exp. 1 (1989-1990), a fourth treatment provided ad libitum access to the HF supplement (SFHF). Supplements contained 88 mg of monensin/kg and the combination of ionophore, minerals and salt (8%) was used to limit intake of the SFHF supplement. Target level of daily consumption of all supplements was .75% of mean BW. Stocking density was increased by 33% (i.e., from 1.24 to 1.65 steers/ha) in Exp. 1 and 3, and by 22 to 44% in Exp. 2 when supplements were fed. Subsequent to grazing wheat pasture in Exp. 2 and 3, feedlot performance and carcass quality (Exp. 2 only) of the cattle were measured. Over the 3-yr period (pooled analysis), mean daily supplement consumption was .65% BW. Daily gains were increased (P < .001) .15 kg by supplementation and were .92, 1.06, and 1.08 kg for CL, HS, and HF, respectively. Daily gains were not influenced (P > .45) by type of energy supplement. Mean supplement conversions (kilograms asfed.kilogram of increased gain-1.hectare-1) were 5.4 for HS and 5.0 for HF and did not differ (P > .95). Subsequent feedlot daily gain was decreased .09 kg (P < .05) by supplementation in Exp. 2 but not in Exp. 3 (P > .80). This supplementation program for growing cattle on wheat pasture allowed stocking density to be increased by approximately one-third and increased daily gains by .15 kg.

Forage intake and digestion by cattle grazing midgrass prairie rangeland or sideoats grama/sweetclover pasture.

In mid-May, beef cattle fitted with esophageal (four steers/pasture) or ruminal and duodenal cannulas (six heifers/pasture; 274 +/- 6 kg BW +/- SE) grazed midgrass prairie (excellent range condition; PRAIRIE) or a seeded mixture of sideoats grama (48% of pasture DM) and sweetclover (6% of pasture DM; Bouteloua curtipendula [Michx.] Torr./Melilotus officinalis [L.] Lam.; PASTURE). Masticate NDF, ADF, and in vitro OM disappearance did not differ (P > .11) between forage types (average = 66.6, 36.1, and 58.8% of OM). Only N differed (P = .02) between PRAIRIE (2.1) and PASTURE (2.4% of OM). Extents of in situ OM and N disappearance were greater (P < .05) and rate of N disappearance between 12 and 36 h was slower (P < .10) from PRAIRIE than from PASTURE masticate. Based on in situ data, the ruminally degraded N: ruminally degraded OM (grams/kilogram) ratio differed (P < .05) between PRAIRIE (22) and PASTURE (25). Ruminal ammonia N concentration (milligrams/deciliter) was less (P = .02) for PRAIRIE (2.8) than for PASTURE (3.8). Forage OM intake and fecal OM output did not differ (P > .72; average = 8,207 and 3,380 g/d), but duodenal OM flow tended (P = .13) to be greater (PRAIRIE = 4,892, PASTURE = 5,170 g/d) in cattle grazing PASTURE. Apparent and true ruminal OM digestion did not differ between forage types (P > .18; average = 38.3 and 48.5%). Nitrogen intake, nonammonia N, and forage N flow at the duodenum were greater (P < .04) for PASTURE (198 vs 171, 242 vs 210, and 162 vs 135 g/d) than for PRAIRIE. Microbial N flow (average = 78 g/d) and microbial efficiency (average = 20 g of microbial N/kg of OM truly fermented) did not differ (P > .25) between forage types. Apparent and true ruminal N digestion did not differ (P > .65; average = -26.6 and 19.3%) between forage types. Flow of nonammonia N was in excess relative to digestible OM intake; hence, digestible OM intake seemed to be first-limiting for performance by cattle grazing either forage type.

Technical note: a double L intestinal cannula for cattle.

A double L-shaped intestinal cannula was developed in an attempt to overcome problems observed previously with simple T-type cannulas. The cannula was constructed from cyclopolyvinyl chloride water pipe fittings. Construction materials were fairly rigid, but by connecting the split cannula pieces with elastic castration bands the cannula had some flexibility. Placing a short cone over the exposed cannula barrel reduced mechanical damage to the intestine. The double L cannula required a much smaller incision in the intestine during surgical insertion than a T-type cannula; it also simplified replacement. Construction is described; use and performance of the cannula has been satisfactory.

Effects of corn gluten feed on forage intake, digestibility and ruminal parameters of cattle fed native grass hay.

Thirty-two beef cows (467 kg) were individually fed native grass hay and supplement for two 14-d periods in each of 2 yr. Supplement treatments and amounts fed (kilograms/day) were negative control (NC), 0, or equal amounts of protein from soybean meal (SBM), .7; a blend of soybean meal and corn gluten feed (SBM/CGF), 1.0; or corn gluten feed (CGF) 1.6. Cows received supplement at 0645 and had ad libitum access to native grass hay from 0700 to 1130 and from 1530 to 2000. Compared with NC, all protein supplements increased (P less than .05) ruminal NH3, propionate and butyrate concentrations at 4 and 25 h postfeeding. Ruminal fluid pH, total VFA and acetate concentrations at 4 and 35 h postfeeding were not affected by supplements. All supplements increased (P less than .01) hay intake as well as hay, acid detergent fiber (ADF) and total diet dry matter (DM) digestibility. Compared to supplemental SBM, feeding CGF reduced (P less than .01) hay intake. Calculated daily intakes of metabolizable energy (ME) were 12, 17, 18, and 17 Mcal for NC, SBM, SBM/CGF and CGF, respectively. Hay intake, DM and ADF digestibility and ME intakes tended to be higher for SBM/CGF than for the average of SBM and CGF fed alone. Intakes of digestible DM and ADF were not altered by protein supplements, suggesting that intake responses were due to increased diet digestibility. Corn gluten feed appears to be an effective source of supplemental protein and energy for cows consuming low-quality roughage.