Differential effect by chronic disease risk: A secondary analysis of the ChooseWell 365 randomized controlled trial.

Objective: Whether employees' health status is associated with the effectiveness of workplace health promotion programs is unknown. The objective of this study was to determine if the effect of a workplace healthy eating intervention differed by baseline chronic disease status. Methods: This was a secondary analysis of a randomized controlled trial conducted September 2016 to February 2018 among US hospital employees to test the effect of a 12-month behavioral intervention (personalized feedback, peer comparisons, and financial incentives) on diet and weight. Participants were classified as having chronic disease (yes/no) based on self-reported hypertension, hyperlipidemia, heart disease, stroke, pre-diabetes, diabetes, cancer or another serious illness. BMI was measured at study visits and calories purchased were measured from cafeteria sales data over 24 months. Mixed models with random effects assessed heterogeneity of treatment effects by chronic disease. Results: Participants (N = 548) were mostly female (79.7 %) and white (81.2 %); 224 (40.9 %) had chronic disease. Among those with chronic disease, intervention participants reduced caloric intake by 74.4 [22.3] kcal more than control, with a smaller difference between intervention and control (-1.9 [18.7] kcal) (three-way p-interaction = 0.02). The effect on BMI for those with chronic disease (0.47 [0.21] kg/m2) indicated weight stability among intervention participants and weight gain among controls while the effect (-0.56 [0.18] kg/m2) for those without chronic disease was the opposite (three-way p-interaction < 0.01). Conclusions: Those with chronic diseases had greater reductions in calories purchased and gained less weight. Employers with limited resources for health promotion might consider tailoring programs to employees at highest risk.

Do children lose and maintain weight easier than adults: a comparison of child and parent weight changes from six months to ten years.

Recent research has shown weight maintenance for obese preadolescent children over 60 and 120 month intervals, while studies on adults consistently show they fail to maintain weight loss. This paper is designed to examine differences in percent overweight changes at 6, 60 and 120 months in obese parents and children from 113 families who participated in randomized controlled outcome studies evaluating family-based behavioral treatment. Analyses showed children had significantly greater changes in percent overweight than their parents at each time point. Chi-Square and Logistic Regression analyses showed children were more likely than their parents at each time point to have percent overweight decreases greater than 20%, with over 20% of the children and less than 1% of the parents showing changes this large. The implications of these results for weight control are discussed. These results suggest there may be differences in the efficacy of treating obesity in children versus adults.

Effects of decreasing sedentary behavior and increasing activity on weight change in obese children.

Obese children 8-12 years old from 61 families were randomized to treatment groups that targeted increased exercise, decreased sedentary behaviors, or both (combined group) to test the influence of reinforcing children to be more active or less sedentary on child weight change. Significant decreases in percentage overweight were observed after 4 months between the sedentary and the exercise groups (-19.9 vs. -13.2). At 1 year, the sedentary group had a greater decrease in percentage overweight than did the combined and the exercise groups (-18.7 vs. -10.3 and -8.7) and greater decrease in percentage of body fat (-4.7 vs. -1.3). All groups improved fitness during treatment and follow-up. Children in the sedentary group increased their liking for high-intensity activity and reported lower caloric intake than did children in the exercise group. These results support the goal of reducing time spent in sedentary activities to improve weight loss.

Ten-year outcomes of behavioral family-based treatment for childhood obesity.

We report 10-year treatment outcomes for obese children in 4 randomized treatment studies. At 10 years, 34% decreased percentage overweight by 20% or more, and 30% were not obese. Significant effects were observed when parents and children were targeted and reinforced for weight loss in comparison with nontargeted controls and for children given lifestyle or aerobic exercise in comparison with a calisthenics control. Thirty-four percent of the variance in change in percentage overweight was predicted from sex, baseline percentage overweight, self-monitoring weight, meals eaten at home, and family and friends' support for eating and exercise. Results show long-term changes in children depend on the treatment, and evidence converges on the importance of the family and other sources of support for eating and activity change.

Effect of weight loss by obese children on long-term growth.

OBJECTIVE: To assess height growth over 10 years in children treated for obesity. DESIGN: Longitudinal, prospective follow-up of a series of randomized, controlled weight control trials. SETTING: Specialized pediatric weight control clinic. PARTICIPANTS: One hundred fifty-eight 6- to 12-year-old obese children who were followed up for 10 years after treatment. INTERVENTIONS: Family-based behavioral weight control. MEASUREMENTS/MAIN RESULTS: At entry the height percentiles of the obese children were significantly higher (71.6 percentile) than same-sex parent (52.0 percentile) or midparent (51.5 percentile) height (an estimate of parental contribution to height). After an average growth of 22.7 cm, children were 2.2 cm taller than their same-sex parent and decreased to an average height percentile of 57.8. Multiple regression analysis showed that child sex, age, baseline height and percent overweight, midparent height, and height change of the child from baseline to 5 years accounted for 94% of the variance in growth. Child percent overweight change made no contribution to predicting height change. Comparison between children obese and nonobese at 10 years showed no differences in growth. CONCLUSIONS: Moderate energy restriction with dietary guidance by overweight children did not negatively influence long-term growth.

Estimation of percent overweight within families.

When height and weight cannot be directly measured percent overweight can be estimated using self-reported height and weight, reports of others for height and weight, and matching of body shape to silhouettes. The present study assessed the relative benefits of each method for estimating percent overweight within families, as well as the potential for using matching of silhouettes for diagnosing obesity. Analysis of the relationship between percent overweight and silhouette ratings showed increases in percent overweight across the silhouettes. Correlations between self- or other-reported and measured percent overweight showed that self-reports correlated very highly with measured reports of percent overweight (r = .97, .96 for parents and children, respectively), but parent reports of their spouses and children percent overweight were lower (r = .87, .87) and child reports of sibling or parent percent overweight were even lower (r = .75, .75). Significant increases in accuracy of diagnosing obesity were observed when the combination of adjusted self-reports and silhouettes were used in comparison to self-reports alone.

Growth in obese children treated for obesity.

This study assesses the growth patterns during a 5-year period in children aged 6 to 12 years treated for obesity using behavioral family-based treatment procedures. Previous studies have suggested a decrease in height velocity after weight reduction, but these results did not consider either the height of the parent or the greater height of obese than nonobese children. Results show that at entry, obese children are taller than their nonobese peers (74th percentile), and that even after 5 years, they remain taller than the norm (65th percentile). Child weight and level of physical maturity accounted for 54% of the variance in predicting baseline height percentile. Entrance height and parental height accounted for 9% of the variance in changes in height percentile, both adjusted for parental height. Weight change did not correlate with growth adjusted for parental height. These results do not suggest that negative effects on height are a long-term side effect of child weight control.

EPR characterization of the cytochrome b-c1 complex from Rhodobacter sphaeroides.

EPR characteristics of cytochrome c1, cytochromes b-565 and b-562, the iron-sulfur cluster, and an antimycin-sensitive ubisemiquinone radical of purified cytochrome b-c1 complex of Rhodobacter sphaeroides have been studied. The EPR specra of cytochrome c1 shows a signal at g = 3.36 flanked with shoulders. The oxidized form of cytochrome b-562 shows a broad EPR signal at g = 3.49, while oxidized cytochrome b-565 shows a signal at g = 3.76, similar to those of two b cytochromes in the mitochondrial complex. The distribution of cytochromes b-565 and b-562 in the isolated complex is 44 and 56%, respectively. Antimycin and 2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinone (DBMIB) have little effect on the g = 3.76 signal, but they cause a slight downfield and upfield shifts of the g = 3.49 signal, respectively. 5-Undecyl-6-hydroxyl-4,7-dioxobenzothiazole (UHDBT) shifts the g = 3.49 signal downfield to g = 3.56 and sharpens the g = 3.76 signal slightly. Myxothiazol causes an upfield shift of both g = 3.49 and g = 3.76 signals. EPR characteristics of the reduced iron-sulfur cluster in bacterial cytochrome b-c1 complex are: gx = 1.8 with a small shoulder at g = 1.76, gy = 1.89 and gz = 2.02, similar to those observed with the mitochondrial enzyme. The gx = 1.8 signal decreased and the shoulder increased concurrently as the redox potential decreased, indicating that the environment of the iron-sulfur cluster is sensitive to the redox state of the complex. UHDBT sharpens the gz and and shifts it downfield from g = 2.02 to 2.03, and shifts gx upfield from g = 1.80 to 1.78. UHDBT also causes an upfield shift of gy but to a much lesser extent compared to the other two signals. Addition of DBMIB causes a downfield shift of the gy from 1.89 to 1.94 and broadens the gx signal with an upfield to g = 1.75. Myxothiazol and antimycin show little effect on the gy and gz signals, but they broaden and shift the gx signal upfield to g = 1.74. However, the myxothiazol effect is partially reversed by UHDBT. An antimycin-sensitive ubisemiquinone radical was detected in the cytochrome b-c1 complex. At pH 8.4, the antimycin-sensitive ubisemiquinone radical has a maximal concentration of 0.66 mol per mol complex at 100 mV.(ABSTRACT TRUNCATED AT 400 WORDS)

Ten-year follow-up of behavioral, family-based treatment for obese children.

Using a prospective, randomized, controlled design, we examined the effects of behavioral family-based treatment on percent overweight and growth over 10 years in obese 6- to 12-year-old children. Obese children and their parents were randomized to three groups that were provided similar diet, exercise, and behavior management training but differed in the reinforcement for weight loss and behavior change. The child and parent group reinforced parent and child behavior change and weight loss, the child group reinforced child behavior change and weight loss, and the nonspecific control group reinforced families for attendance. Children in the child and parent group showed significantly greater decreases in percent overweight after 5 and 10 years (-11.2% and -7.5%, respectively) than children in the nonspecific control group (+ 7.9% and + 14.3%, respectively). Children in the child group showed increases in percent overweight after 5 and 10 years (+ 2.7% and + 4.5%, respectively) that were midway between those for the child and parent and nonspecific groups and not significantly different from either. At 10 years, child height was related strongly to the height of the parent of the same sex (r = .78 children were 1.8 cm taller than their parents, with no differences in height between groups.

Five-year follow-up of family-based behavioral treatments for childhood obesity.

This article presents the 5-year outcome of family-based behavioral treatment of obesity for 6- to 12-year-old children in 162 families across 4 treatment outcome studies. Results suggest that treatments that use (a) conjoint targeting and reinforcement of child and parent behavior or (b) reciprocal targeting and reinforcement of children and parents are associated with the best child outcomes. Predictors of child success include self-monitoring, changing eating behavior, praise, and change in parent percent overweight. Parental outcome is predicted by self-monitoring weight, baseline parent percent overweight, and participation in fewer subsequent weight control programs.

The effect of weight control on lipid changes in obese children.

The effects of weight change on serum lipid changes were assessed in a sample of 56 obese children randomly assigned to family-based behavioral obesity treatment vs controls given no treatment. Fasting serum lipid levels, height, weight, and fitness were measured at program entry and after 6 months of treatment. Children assigned to treatment showed significantly greater relative weight and weight changes than children in the control group, and the weight changes were significantly related to reductions in fasting serum triglyceride and total serum cholesterol levels and increases in high-density lipoprotein serum cholesterol. Results after 5 years of follow-up available for a small sample of treated children showed that a change in relative weight and fitness from 6 months to 5 years was also associated with changes in the high-density lipoprotein level. These results suggest that weight control in obese preadolescent children may be associated with improvement in lipoprotein profiles.

Perception of eating and exercise in children as a function of child and parent weight status.

The present study assessed the effects of child body weight (obese/lean) and familial loading for obesity (two obese parents/two lean parents) on the psychophysics of sweetness, fatness and exercise workloads, as well as subjective ratings of foods varying in sugar and fat and activities varying in energy expenditure. Children were measured in a baseline state and at 6 months after the obese children had participated in a family-based behavioral weight control program. No differences between obese and lean children in perceptual ratings were observed. However, offspring of obese or lean parents differed on intensity rating, food palatability and activity enjoyment ratings. Intensity ratings for sweetness in offspring of obese parents was increased, with a similar trend for intensity ratings for fatness. Offspring of obese parents rated all foods and activities with lower palatability and enjoyment ratings than offspring of lean parents. After a 6 month family-based behavioral weight loss treatment, obese children had significant decreases in percent overweight while lean children remained stable. Changes in the pattern of food ratings were observed for the obese children, with a reduction in liking for foods high in fat and/or sugar, and an increase in ratings for food slower in fat and sugar. The effects of parental obesity on food and exercise intensity ratings and hedonic ratings were maintained. Overall, these results suggest parental weight influences behavioral factors related to obesity in children.

An antimycin-insensitive succinate-cytochrome c reductase activity in pure reconstitutively active succinate dehydrogenase.

Antimycin-insensitive succinate-cytochrome c reductase activity has been detected in pure, reconstitutively active succinate dehydrogenase. The enzyme catalyzes electron transfer from succinate to cytochrome c at a rate of 0.7 mumole succinate oxidized per min per mg protein, in the presence of 100 microM cytochrome c. This activity, which is about 2% of that of reconstitutive (the ability of succinate dehydrogenase to reconstitute with coenzyme ubiquinone-binding proteins (QPs) to form succinate-ubiquinone reductase) or succinate-phenazine methosulfate activity in the preparation, differs from antimycin-insensitive succinate-cytochrome c reductase activity detected in submitochondrial particles or isolated succinate-cytochrome c reductase. The Km for cytochrome c for the former is too high to be measured. The Km for the latter is about 4.4 microM, similar to that of antimycin-sensitive succinate-cytochrome c activity in isolated succinate-cytochrome c reductase, suggesting that antimycin-insensitive succinate-cytochrome c activity of succinate-cytochrome c reductase probably results from incomplete inhibition by antimycin. Like reconstitutive activity of succinate dehydrogenase, the antimycin-insensitive succinate-cytochrome c activity of succinate dehydrogenase is sensitive to oxygen; the half-life is about 20 min at 0 degrees C at a protein concentration of 23 mg/ml. In the presence of QPs, the antimycin-insensitive succinate-cytochrome c activity of succinate dehydrogenase disappears and at the same time a thenoyltrifluoroacetone-sensitive succinate-ubiquinone reductase activity appears. This suggests that antimycin-insensitive succinate-cytochrome c reductase activity of succinate dehydrogenase appears when succinate dehydrogenase is detached from the membrane or from QPs. Reconstitutively active succinate dehydrogenase oxidizes succinate using succinylated cytochrome c as electron acceptor, suggesting that a low potential intermediate (radical) may be involved. This suggestion is confirmed by the detection of an unknown radical by spin trapping techniques. When a spin trap, alpha-phenyl-N-tert-butylnitrone (PBN), is added to a succinate oxidizing system containing reconstitutively active succinate dehydrogenase, a PBN spin adduct is generated. Although this PBN spin adduct is identical to that generated by xanthine oxidase, indicating that a perhydroxy radical might be involved, the insensitivity of this antimycin-insensitive succinate-cytochrome c reductase activity to superoxide dismutase and oxygen questions the nature of this observed radical.

Prediction of mature cow carcass composition from live animal measurements.

Two trials were conducted with mature cows to develop prediction equations for estimating carcass composition from live animal measurements. Trial 1 involved 82 animals that were used to develop these equations. Subjective criteria (frame and condition scores) also were utilized along with live weight in development of prediction equations. Equations using subjective independent variables predicting energy and carcass tissue weights apparently were slightly more variable in precision (R2 = .69 to .91) than those developed with objective variables (R2 = .74 to .90). Equations predicting percent compositions from subjective variables had lower coefficients of determination (R2 = .49 to .76) than those developed with objective variables (R2 = .63 to .83). Forty-one animals similar to those used in Trial 1 served as a test group in Trial 2. Measured carcass composition was regressed on composition predicted by equations developed in Trial 1. These equations generally had slopes and intercepts with confidence intervals that included 0 and 1, respectively, indicating that most of the prediction equations were unbiased. This work indicates that carcass composition can be predicted from both objective and subjective measurements, with some accuracy and precision.

The EPR spectra of the cytochrome b-c1 complex of Rhodopseudomonas sphaeroides.

The purified cytochrome b-c1 complex of Rhodopseudomonas sphaeroides has two b cytochromes distinguishable by optical, thermodynamic and electron paramagnetic resonance criteria (gz values are approximately equal to 3.75 and approximately equal to 3.4). EPR features typical of a Rieske iron sulfur cluster (g values of 2.03 1.90 and 1.81) and a c1 type cytochrome (g approximately equal to 3.4) were also observed. The b and c1 cytochromes were individually purified from the complex. The cytochrome c1 retained its native EPR spectrum. The b cytochrome lost over 90% of the intensity from the 'b566 type' heme site (g approximately equal to 3.75), while the 'b561 type' heme site (g approximately equal to 3.4) retained its native EPR spectrum.

Growth patterns of Angus, Hereford and shorthorn cattle. I. Comparison of inbred and noninbred lines, changes in patterns over time and effects of level of inbreeding and reproductive performance.

Mature weight (A) and rate of maturing (K) were estimated for 283 Angus, 140 Hereford and 280 Shorthorn cows utilizing the asymptotic regression equation Yt=A(1-Be-Kt). The Yt was weight of the individual at age t; and B was an estimate related to early life weight changes and provided for a Y-intercept (A-B). Each breed consisted of four inbred and two noninbred lines. Regression of estimated growth curve parameters on levels of inbreeding of the individuals and of their dams and effects of early reproductive performance (EREPRO) were studied as sources of variation in addition to line differences and trends in line values over years. Considered jointly with inbreeding of the dam and with EREPRO, inbreeding of the individual was negatively related (P less than .01) to estimates of A in the three breeds and unrelated (P greater than .10) to estimates of K. Each 1% increase in inbreeding of the individual was associated with about 2 kg decrease in estimated mature weight. An increase in inbreeding of the dam of an individual was negatively related to estimates of K in Angus (P less than .01) and Shorthorns (P less than .05). Inbreeding of dams was positively related to estimates of A in the three breeds, but only in Shorthorns could the relationship be declared significant. Estimates of A were about 46 kg heavier and estimates of K about .010 less for each year an individual failed to produce a calf during her first three opportunities. In general, A values declined in all three breeds during the study. Significant changes were observed in all Hereford analyses and after inbreeding was included in the Angus analysis, while inclusion of inbreeding in the Shorthorn analysis caused the change to become unimportant. The K values increased in all three breeds during the study; however, the change could be declared significant only in Herefords.

Growth patterns of Angus, Hereford and shorthorn cattle. II. Relationship of growth patterns of dams with progeny performance.

Relationships between estimated growth curve parameters of dams and performance traits of their progeny were studied in Angus, Hereford and Shorthorn herds, each divided into four inbred and two noninbred lines. Growth curve parameters were calculated from the growth function Yt=A(1-Be-Kt), where Yt was weight at age t, A was estimated mature weight, B was an estimate related to early life weight changes and provided for a Y-intercept term and K was estimated general rate of maturing. Least-squares analyses of progeny variables were calculated separately for each breed and sex. Line differences did not influence any of the progeny variables except weaning type score of Shorthorn males (P less than .01). Birth year exerted a curvilinear effect on birth weights of Angus female progeny (P less than .01), a linear influence on 205-d weights of Shorthorn male progeny (P less than .05) and a curvilinear effect on weaning type scores of Angus and Shorthorn male progeny (P less than .01). Regression coefficients on weaning age indicated that calves born earlier in the calving season had lighter birth weights and that older calves at weaning received higher type scores. Inbreeding of the progeny negatively influenced (P less than .05) birth weights of Angus male and Hereford female progeny. Hereford male and Shorthorn female 205-d weights were negatively affected (P less than .05) by inbreeding, while weaning type scores of Shorthorn female progeny were negatively influenced (P less than .001) by inbreeding. Regression coefficients of all progeny variables on inbreeding in all analyses indicated negative trends.(ABSTRACT TRUNCATED AT 250 WORDS)