Steroidogenesis by bovine theca interna in an in vitro perifusion system.

The aims of these studies were: to examine the steroidogenic responses of perifused bovine theca interna to varying flow rates of media and varying amounts of luteinizing hormone (LH), and to compare the steroidogenic outputs of theca interna from follicles of differing size and health with those of other ovarian tissues. The results showed that the outputs of androstenedione by thecae interna from healthy but not atretic follicles, with or without stimulation by LH, were amplified by the flow rate of media. Steroidogenesis by perifused theca interna was also influenced by the mass and concentration of LH as well as by the duration of exposure to LH. When expressed on a per unit mass basis, the outputs of androstenedione from LH-primed thecae interna from small (2-5.5 mm diameter), medium (6-9.5 mm diameter) and large (greater than or equal to 10 mm diameter) healthy follicles were comparable. But when the above data were expressed per total mass of theca interna, the androstenedione output increased significantly with increasing follicular diameter (P less than 0.01). Under the experimental conditions employed, the fraction of androstenedione produced by thecal tissue as a percentage of the total output of progesterone, androstenedione, testosterone and estradiol was 82%, whereas the progesterone, testosterone and estradiol fractions were 1%, 15% and 2%, respectively. By contrast, the granulosa cell output of progesterone, androstenedione, testosterone and estradiol were 79%, 0%, 0% and 21%, respectively. When this cell type was supplied with saturating amounts of androstenedione, it contributed greater than or equal to 90% of the total quantity of estradiol by the two cell types in isolation.

Changes in gonadotrophin secretion and ovarian antral follicular activity in seasonally breeding sheep throughout the year.

Overall, significantly more antral follicles greater than or equal to 1 mm diameter were present in Romney ewes during anoestrus than in the breeding season (anoestrus, 35 +/- 3 (mean +/- s.e.m.) follicles per ewe, 23 sheep; Day 9-10 of oestrous cycle, 24 +/- 1 follicles per ewe, 22 sheep; P less than 0.01), although the mean numbers of preovulatory-sized follicles (greater than or equal to 5 mm diam.) were similar (anoestrus, 1.3 +/- 0.2 per ewe; oestrous cycle, 1.0 +/- 0.1 per ewe). The ability of ovarian follicles to synthesize oestradiol did not differ between anoestrus and the breeding season as assessed from the levels of extant aromatase enzyme activity in granulosa cells and steroid concentrations in follicular fluid. Although the mean plasma concentration of LH did not differ between anoestrus and the luteal phase of the breeding season, the pattern of LH secretion differed markedly; on Day 9-10 of the oestrous cycle there were significantly more (P less than 0.001) high-amplitude LH peaks (i.e. greater than or equal to 1 ng/ml) in plasma and significantly fewer (P less than 0.001) low amplitude peaks (less than 1 ng/ml) than in anoestrous ewes. Moreover, the mean concentrations of FSH and prolactin were significantly lower during the luteal phase of the cycle than during anoestrus (FSH, P less than 0.05, prolactin, P less than 0.001). It is concluded that, in Romney ewes, the levels of antral follicular activity change throughout the year in synchrony with the circannual patterns of prolactin and day-length. Also, these data support the notion that anovulation during seasonal anoestrus is due to a reduced frequency of high-amplitude LH discharges from the pituitary gland.