ABSTRACT
Benzoporphyrin derivatives (BPDs) are photosensitizers, which fluoresce strongly at 690 nm, and may be candidates for various applications of photodynamic therapy (PDT). Fluorescence-activated cell sorting (FACS) analysis, subsequent to ultraviolet light excitation, revealed pronounced differences in red fluorescence between leukemic cell lines (HL60, K562 and L1210), leukemic clinical isolates, and normal human or murine bone marrow cells incubated with BPD. These observed differences in BPD-mediated fluorescence provide the rationale for sorting leukemic from normal cells via FACS or may constitute a novel method for extracorporeal purging of remission marrow by photodynamic therapy in autologous bone marrow transplantation.
Subject(s)
Biological Transport , Leukemia/metabolism , Monocytes/metabolism , Porphyrins/metabolism , Radiation-Sensitizing Agents/metabolism , Tumor Cells, Cultured/metabolism , Animals , Bone Marrow/metabolism , Cell Line , Female , Flow Cytometry , Humans , In Vitro Techniques , Kinetics , Leukemia/blood , Leukemia L1210/metabolism , Mice , Mice, Inbred DBA , Reference Values , Spleen/metabolism , Structure-Activity RelationshipABSTRACT
The majority of patients presenting for resection of phaeochromocytoma require some form of antihypertensive therapy during operation. Phentolamine and sodium nitroprusside have both been used successfully for this purpose, but each has disadvantages. This report describes the use of intravenous nitroglycerin, a rapidly acting venodilator with no appreciable toxicity, as the sole antihypertensive agent in two patients with phaeochromocytoma and partial alpha adrenergic blockade. Hypertensive episodes were quickly and effectively controlled in each case. There were no hypotensive periods and no side effects.
