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1.
PLoS Genet ; 8(7): e1002655, 2012.
Article in English | MEDLINE | ID: mdl-22829771

ABSTRACT

Stature is a classical and highly heritable complex trait, with 80%-90% of variation explained by genetic factors. In recent years, genome-wide association studies (GWAS) have successfully identified many common additive variants influencing human height; however, little attention has been given to the potential role of recessive genetic effects. Here, we investigated genome-wide recessive effects by an analysis of inbreeding depression on adult height in over 35,000 people from 21 different population samples. We found a highly significant inverse association between height and genome-wide homozygosity, equivalent to a height reduction of up to 3 cm in the offspring of first cousins compared with the offspring of unrelated individuals, an effect which remained after controlling for the effects of socio-economic status, an important confounder (χ(2) = 83.89, df = 1; p = 5.2 × 10(-20)). There was, however, a high degree of heterogeneity among populations: whereas the direction of the effect was consistent across most population samples, the effect size differed significantly among populations. It is likely that this reflects true biological heterogeneity: whether or not an effect can be observed will depend on both the variance in homozygosity in the population and the chance inheritance of individual recessive genotypes. These results predict that multiple, rare, recessive variants influence human height. Although this exploratory work focuses on height alone, the methodology developed is generally applicable to heritable quantitative traits (QT), paving the way for an investigation into inbreeding effects, and therefore genetic architecture, on a range of QT of biomedical importance.


Subject(s)
Body Height/genetics , Consanguinity , Genes, Recessive , Genetic Heterogeneity , Quantitative Trait, Heritable , Adult , Aged , Databases, Genetic , Family , Female , Genome-Wide Association Study , Homozygote , Humans , Male , Middle Aged , Polymorphism, Single Nucleotide
2.
Biol Psychiatry ; 70(6): 513-8, 2011 Sep 15.
Article in English | MEDLINE | ID: mdl-21529783

ABSTRACT

BACKGROUND: Given moderately strong genetic contributions to variation in alcoholism and heaviness of drinking (50% to 60% heritability) with high correlation of genetic influences, we have conducted a quantitative trait genome-wide association study (GWAS) for phenotypes related to alcohol use and dependence. METHODS: Diagnostic interview and blood/buccal samples were obtained from sibships ascertained through the Australian Twin Registry. Genome-wide single nucleotide polymorphism (SNP) genotyping was performed with 8754 individuals (2062 alcohol-dependent cases) selected for informativeness for alcohol use disorder and associated quantitative traits. Family-based association tests were performed for alcohol dependence, dependence factor score, and heaviness of drinking factor score, with confirmatory case-population control comparisons using an unassessed population control series of 3393 Australians with genome-wide SNP data. RESULTS: No findings reached genome-wide significance (p = 8.4 × 10(-8) for this study), with lowest p value for primary phenotypes of 1.2 × 10(-7). Convergent findings for quantitative consumption and diagnostic and quantitative dependence measures suggest possible roles for a transmembrane protein gene (TMEM108) and for ANKS1A. The major finding, however, was small effect sizes estimated for individual SNPs, suggesting that hundreds of genetic variants make modest contributions (1/4% of variance or less) to alcohol dependence risk. CONCLUSIONS: We conclude that 1) meta-analyses of consumption data may contribute usefully to gene discovery; 2) translation of human alcoholism GWAS results to drug discovery or clinically useful prediction of risk will be challenging; and 3) through accumulation across studies, GWAS data may become valuable for improved genetic risk differentiation in research in biological psychiatry (e.g., prospective high-risk or resilience studies).


Subject(s)
Alcohol Drinking/genetics , Alcoholism/genetics , Genetic Predisposition to Disease/genetics , Genome-Wide Association Study/statistics & numerical data , Quantitative Trait Loci/genetics , Alcoholism/diagnosis , Case-Control Studies , Genotype , Humans , Phenotype , Polymorphism, Single Nucleotide , Residence Characteristics
3.
Genome Res ; 21(6): 821-9, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21518737

ABSTRACT

Genetic and fossil evidence supports a single, recent (<200,000 yr) origin of modern Homo sapiens in Africa, followed by later population divergence and dispersal across the globe (the "Out of Africa" model). However, there is less agreement on the exact nature of this migration event and dispersal of populations relative to one another. We use the empirically observed genetic correlation structure (or linkage disequilibrium) between 242,000 genome-wide single nucleotide polymorphisms (SNPs) in 17 global populations to reconstruct two key parameters of human evolution: effective population size (N(e)) and population divergence times (T). A linkage disequilibrium (LD)-based approach allows changes in human population size to be traced over time and reveals a substantial reduction in N(e) accompanying the "Out of Africa" exodus as well as the dramatic re-expansion of non-Africans as they spread across the globe. Secondly, two parallel estimates of population divergence times provide clear evidence of population dispersal patterns "Out of Africa" and subsequent dispersal of proto-European and proto-East Asian populations. Estimates of divergence times between European-African and East Asian-African populations are inconsistent with its simplest manifestation: a single dispersal from the continent followed by a split into Western and Eastern Eurasian branches. Rather, population divergence times are consistent with substantial ancient gene flow to the proto-European population after its divergence with proto-East Asians, suggesting distinct, early dispersals of modern H. sapiens from Africa. We use simulated genetic polymorphism data to demonstrate the validity of our conclusions against alternative population demographic scenarios.


Subject(s)
Demography , Evolution, Molecular , Genome, Human/genetics , Linkage Disequilibrium , Polymorphism, Single Nucleotide/genetics , Population Dynamics , Computer Simulation , Gene Frequency , Humans , Models, Genetic
4.
Biol Psychol ; 85(2): 306-17, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20691247

ABSTRACT

Variation in personality traits is 30-60% attributed to genetic influences. Attempts to unravel these genetic influences at the molecular level have, so far, been inconclusive. We performed the first genome-wide association study of Cloninger's temperament scales in a sample of 5117 individuals, in order to identify common genetic variants underlying variation in personality. Participants' scores on Harm Avoidance, Novelty Seeking, Reward Dependence, and Persistence were tested for association with 1,252,387 genetic markers. We also performed gene-based association tests and biological pathway analyses. No genetic variants that significantly contribute to personality variation were identified, while our sample provides over 90% power to detect variants that explain only 1% of the trait variance. This indicates that individual common genetic variants of this size or greater do not contribute to personality trait variation, which has important implications regarding the genetic architecture of personality and the evolutionary mechanisms by which heritable variation is maintained.


Subject(s)
Personality/genetics , Twins/genetics , Adult , Age Factors , Exploratory Behavior , Female , Genome-Wide Association Study , Genotype , Humans , Life Style , Male , Middle Aged , Reward , Surveys and Questionnaires , Temperament
5.
Am J Hum Genet ; 87(2): 297-305, 2010 Aug 13.
Article in English | MEDLINE | ID: mdl-20691402

ABSTRACT

Australia was probably settled soon after modern humans left Africa, but details of this ancient migration are not well understood. Debate centers on whether the Pleistocene Sahul continent (composed of New Guinea, Australia, and Tasmania) was first settled by a single wave followed by regional divergence into Aboriginal Australian and New Guinean populations (common origin) or whether different parts of the continent were initially populated independently. Australia has been the subject of relatively few DNA studies even though understanding regional variation in genomic structure and diversity will be important if disease-association mapping methods are to be successfully evaluated and applied across populations. We report on a genome-wide investigation of Australian Aboriginal SNP diversity in a sample of participants from the Riverine region. The phylogenetic relationship of these Aboriginal Australians to a range of other global populations demonstrates a deep common origin with Papuan New Guineans and Melanesians, with little evidence of substantial later migration until the very recent arrival of European colonists. The study provides valuable and robust insights into an early and important phase of human colonization of the globe. A broader survey of Australia, including diverse geographic sample populations, will be required to fully appreciate the continent's unique population history and consequent genetic heritage, as well as the importance of both to the understanding of health issues.


Subject(s)
Genetic Variation , Genome, Human/genetics , Native Hawaiian or Other Pacific Islander/genetics , Phylogeny , Australia , Genetic Markers , Genetics, Population , Geography , Humans , Polymorphism, Single Nucleotide/genetics , Principal Component Analysis
6.
Nat Genet ; 42(7): 565-9, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20562875

ABSTRACT

SNPs discovered by genome-wide association studies (GWASs) account for only a small fraction of the genetic variation of complex traits in human populations. Where is the remaining heritability? We estimated the proportion of variance for human height explained by 294,831 SNPs genotyped on 3,925 unrelated individuals using a linear model analysis, and validated the estimation method with simulations based on the observed genotype data. We show that 45% of variance can be explained by considering all SNPs simultaneously. Thus, most of the heritability is not missing but has not previously been detected because the individual effects are too small to pass stringent significance tests. We provide evidence that the remaining heritability is due to incomplete linkage disequilibrium between causal variants and genotyped SNPs, exacerbated by causal variants having lower minor allele frequency than the SNPs explored to date.


Subject(s)
Body Height/genetics , Genetic Predisposition to Disease/genetics , Genome-Wide Association Study/methods , Polymorphism, Single Nucleotide , Adolescent , Adult , Aged , Aged, 80 and over , Algorithms , Female , Gene Frequency , Genome, Human , Genotype , Humans , Linkage Disequilibrium , Logistic Models , Male , Middle Aged , Models, Genetic , Young Adult
7.
Am J Hum Genet ; 86(1): 88-92, 2010 Jan.
Article in English | MEDLINE | ID: mdl-20045101

ABSTRACT

Abnormal expansion or depletion of particular lymphocyte subsets is associated with clinical manifestations such as HIV progression to AIDS and autoimmune disease. We sought to identify genetic predictors of lymphocyte levels and reasoned that these may play a role in immune-related diseases. We tested 2.3 million variants for association with five lymphocyte subsets, measured in 2538 individuals from the general population, including CD4+ T cells, CD8+ T cells, CD56+ natural killer (NK) cells, and the derived measure CD4:CD8 ratio. We identified two regions of strong association. The first was located in the major histocompatibility complex (MHC), with multiple SNPs strongly associated with CD4:CD8 ratio (rs2524054, p = 2.1 x 10(-28)). The second region was centered within a cluster of genes from the Schlafen family and was associated with NK cell levels (rs1838149, p = 6.1 x 10(-14)). The MHC association with CD4:CD8 replicated convincingly (p = 1.4 x 10(-9)) in an independent panel of 988 individuals. Conditional analyses indicate that there are two major independent quantitative trait loci (QTL) in the MHC region that regulate CD4:CD8 ratio: one is located in the class I cluster and influences CD8 levels, whereas the second is located in the class II cluster and regulates CD4 levels. Jointly, both QTL explained 8% of the variance in CD4:CD8 ratio. The class I variants are also strongly associated with durable host control of HIV, and class II variants are associated with type-1 diabetes, suggesting that genetic variation at the MHC may predispose one to immune-related diseases partly through disregulation of T cell homeostasis.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Diabetes Mellitus, Type 1/immunology , HIV Infections/immunology , HIV-1/metabolism , Adolescent , CD4-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/cytology , Child , Diabetes Mellitus, Type 1/blood , Genetic Predisposition to Disease , Genetic Variation , Genotype , HIV Infections/blood , Humans , Killer Cells, Natural/immunology , Lymphocyte Count , Quantitative Trait Loci , Risk
8.
Am J Hum Genet ; 85(5): 750-5, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19896111

ABSTRACT

Hair morphology is highly differentiated between populations and among people of European ancestry. Whereas hair morphology in East Asian populations has been studied extensively, relatively little is known about the genetics of this trait in Europeans. We performed a genome-wide association scan for hair morphology (straight, wavy, curly) in three Australian samples of European descent. All three samples showed evidence of association implicating the Trichohyalin gene (TCHH), which is expressed in the developing inner root sheath of the hair follicle, and explaining approximately 6% of variance (p=1.5x10(-31)). These variants are at their highest frequency in Northern Europeans, paralleling the distribution of the straight-hair EDAR variant in Asian populations.


Subject(s)
Genetic Variation , Hair/anatomy & histology , Protein Precursors/genetics , White People/genetics , Adolescent , Adult , Alleles , Amino Acid Sequence , Amino Acid Substitution , Australia/ethnology , Child , Chromosomes, Human, Pair 1 , Cohort Studies , Female , Gene Frequency , Genetic Markers , Genetics, Population , Genome, Human , Genome-Wide Association Study , Genotype , Geography , Hair Follicle/anatomy & histology , Haplotypes , Humans , Intermediate Filament Proteins , Karyotyping , Linkage Disequilibrium , Male , Meta-Analysis as Topic , Methionine/metabolism , Molecular Sequence Data , Nuclear Family , Polymorphism, Single Nucleotide , Young Adult
9.
Econ Hum Biol ; 7(3): 294-306, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19818695

ABSTRACT

Height is correlated with risk to certain diseases and various socio-economic outcomes. As an easy to observe and measure trait, it has been a classic paradigm in the emergence of fundamental concepts regarding inheritance and genetics. Resemblances in height between relatives suggest that 80% of height variation is under genetic control with the rest controlled by environmental factors such as diet and disease exposure. Nearly a century ago it was recognised that many genes were likely to be involved but it is only with recent advances in technology that it has become possible to comprehensively search the human genome for DNA variants that control height. About 50 genes and regions of the genome have been associated with height to date. These begin to explain the biological basis of height, its links to disease and aid our understanding of the evolution of human height. The genes discovered so far have a very small individual effect and hundreds, maybe thousands, more of even smaller effects are still lost in the genome. Despite a successful start to height gene mapping, there remain considerable theoretical, technological, and statistical hurdles to be overcome in order to unravel its full genetic basis.


Subject(s)
Body Height/genetics , Biological Evolution , Chromosome Mapping , Diet , Environment , Genome-Wide Association Study , Health Status , Humans , Racial Groups , Sexual Behavior
10.
Nat Genet ; 41(11): 1173-5, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19820699

ABSTRACT

We report a genome-wide association study to iron status. We identify an association of SNPs in TPMRSS6 to serum iron (rs855791, combined P = 1.5 x 10(-20)), transferrin saturation (combined P = 2.2 x 10(-23)) and erythrocyte mean cell volume (MCV, combined P = 1.1 x 10(-10)). We also find suggestive evidence of association with blood hemoglobin levels (combined P = 5.3 x 10(-7)). These findings demonstrate the involvement of TMPRSS6 in control of iron homeostasis and in normal erythropoiesis.


Subject(s)
Erythrocyte Volume , Erythrocytes/cytology , Erythrocytes/metabolism , Homeostasis , Iron/blood , Membrane Proteins/genetics , Polymorphism, Single Nucleotide , Serine Endopeptidases/genetics , Chromosomes, Human, Pair 22 , Genome-Wide Association Study , Humans , Transferrin/metabolism
11.
Genome Res ; 19(5): 804-14, 2009 May.
Article in English | MEDLINE | ID: mdl-19265028

ABSTRACT

Population structure can provide novel insight into the human past, and recognizing and correcting for such stratification is a practical concern in gene mapping by many association methodologies. We investigate these patterns, primarily through principal component (PC) analysis of whole genome SNP polymorphism, in 2099 individuals from populations of Northern European origin (Ireland, United Kingdom, Netherlands, Denmark, Sweden, Finland, Australia, and HapMap European-American). The major trends (PC1 and PC2) demonstrate an ability to detect geographic substructure, even over a small area like the British Isles, and this information can then be applied to finely dissect the ancestry of the European-Australian and European-American samples. They simultaneously point to the importance of considering population stratification in what might be considered a small homogeneous region. There is evidence from F(ST)-based analysis of genic and nongenic SNPs that differential positive selection has operated across these populations despite their short divergence time and relatively similar geographic and environmental range. The pressure appears to have been focused on genes involved in immunity, perhaps reflecting response to infectious disease epidemic. Such an event may explain a striking selective sweep centered on the rs2508049-G allele, close to the HLA-G gene on chromosome 6. Evidence of the sweep extends over a 8-Mb/3.5-cM region. Overall, the results illustrate the power of dense genotype and sample data to explore regional population variation, the events that have crafted it, and their implications in both explaining disease prevalence and mapping these genes by association.


Subject(s)
Selection, Genetic , White People/genetics , Europe , Genetics, Population , Genome-Wide Association Study , Genotype , Geography , Humans , Polymorphism, Single Nucleotide , Principal Component Analysis
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