Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Immunol ; 164(7): 3870-7, 2000 Apr 01.
Article in English | MEDLINE | ID: mdl-10725749

ABSTRACT

The major cause of death in cystic fibrosis (CF) is chronic lung disease associated with persistent infection by the bacterium, Pseudomonas aeruginosa. S100A8, an S-100 calcium-binding protein with chemotactic activity, is constitutively expressed in the lungs and serum of CF patients. Levels of S100A8 mRNA were found to be three to four times higher in the lungs of mice carrying the G551D mutation in CF transmembrane conductance regulator compared with littermate controls. Intravenous injection of bacterial LPS induced S100A8 mRNA in the lung to a greater extent in G551D mice than in wild-type littermates. Localization of S100A8 mRNA and protein in the lung indicate that it is a marker for neutrophil accumulation. Bone marrow-derived macrophages from G551D mice were shown to also exhibit hypersensitivity to LPS, measured by induction of TNF-alpha. These results provide evidence that the pathology of CF relates to abnormal regulation of the immune system.


Subject(s)
Amino Acid Substitution/genetics , Cystic Fibrosis/genetics , Cystic Fibrosis/pathology , Lung/pathology , Macrophages/pathology , Mutation, Missense , Animals , Aspartic Acid/genetics , Biomarkers , Bone Marrow Cells/immunology , Bone Marrow Cells/metabolism , Bone Marrow Cells/pathology , Cell Movement , Cystic Fibrosis/immunology , Cystic Fibrosis/metabolism , Disease Models, Animal , Glycine/genetics , Inflammation/genetics , Inflammation/immunology , Inflammation/metabolism , Inflammation/pathology , Injections, Intravenous , Lipopolysaccharides/administration & dosage , Lung/metabolism , Macrophages/immunology , Macrophages/metabolism , Mice , Mice, Mutant Strains , Neutrophils/pathology , RNA, Messenger/biosynthesis
SELECTION OF CITATIONS
SEARCH DETAIL
...