Aryl (ß,ß',ß″-Trifluoro)-tert-butyl: A Candidate Motif for the Discovery of Bioactives.

The (ß,ß',ß″-trifluoro)-tert-butyl (TFTB) group has received very little attention in the literature. This work presents a direct synthesis of this group and explores its properties. The TFTB group arises when the methyl groups of a tert-butyl moiety are exchanged for fluoromethyl groups. Sequential fluoromethylations result in a decrease of Log P (increasing hydrophilicity), ultimately by 1.7 Log P units in the TFTB group relative to that of tert-butyl benzene itself. A focus is placed on synthetic transformations, conformational analysis, and metabolism of the TFTB group in the context of presenting a favorable profile as a motif for the discovery of bioactives.

Objective Measures of Strain and Subjective Muscle Soreness Differ Between Positional Groups and Season Phases in American College Football.

PURPOSE: To assess objective strain and subjective muscle soreness in "Bigs" (offensive and defensive line), "Combos" (tight ends, quarterbacks, line backers, and running backs), and "Skills" (wide receivers and defensive backs) in American college football players during off-season, fall camp, and in-season phases. METHODS: Twenty-three male players were assessed once weekly (3-wk off-season, 4-wk fall camp, and 3-wk in-season) for hydroperoxides (free oxygen radical test [FORT]), antioxidant capacity (free oxygen radical defense test [FORD]), oxidative stress index (OSI), countermovement-jump flight time, Reactive Strength Index (RSI) modified, and subjective soreness. Linear mixed models analyzed the effect of a 2-within-subject-SD change between predictor and dependent variables. RESULTS: Compared to fall camp and in-season phases, off-season FORT (P ≤ .001 and <.001), FORD (P ≤ .001 and <.001), OSI (P ≤ .001 and <.001), flight time (P ≤ .001 and <.001), RSI modified (P ≤ .001 and <.001), and soreness (P ≤ .001 and <.001) were higher for "Bigs," whereas FORT (P ≤ .001 and <.001) and OSI (P = .02 and <.001) were lower for "Combos." FORT was higher for "Bigs" compared to "Combos" in all phases (P ≤ .001, .02, and .01). FORD was higher for "Skills" compared with "Bigs" in off-season (P = .02) and "Combos" in-season (P = .01). OSI was higher for "Bigs" compared with "Combos" (P ≤ .001) and "Skills" (P = .01) during off-season and to "Combos" in-season (P ≤ .001). Flight time was higher for "Skills" in fall camp compared with "Bigs" (P = .04) and to "Combos" in-season (P = .01). RSI modified was higher for "Skills" during off-season compared with "Bigs" (P = .02) and "Combos" during fall camp (P = .03), and in-season (P = .03). CONCLUSION: Off-season American college football training resulted in higher objective strain and subjective muscle soreness in "Bigs" compared with fall camp and during in-season compared with "Combos" and "Skills" players.

Uphill sprinting load- and force-velocity profiling: Assessment and potential applications.

This study aimed to quantify the validity and reliability of load-velocity (LV) relationship of hill sprinting using a range of different hill gradients and to describe the effect of hill gradient on sprint performance. Twenty-four collegiate-level athletes performed a series of maximal sprints on either flat terrain or hills of gradients 5.2, 8.8 and 17.6%. Velocity-time curves were recorded using a radar device. LV relationships were established using the maximal velocity achieved in each sprinting condition, whilst force-velocity-power (FVP) profiles were established using only the flat terrain sprint. LV profiles were shown to be valid (R2 = 0.99) and reliable (TE < 4.4%). For every 1-degree increase in slope, subjects' velocity decreased by 1.7 ± 0.1% on average. All the slopes used represented low resistance relative to the entire LV spectrum (<25% velocity loss). Subjects who exhibited greater horizontal force output at higher velocities on flat terrain were most affected by the gradient of the hill. Hills of gradients up to 17.6% do not provide sufficient resistance to optimize power development. However, such hills could be used to develop late-stage technical ability, due to the prolonged horizontally oriented body position that occurs as subjects attempt to overcome the acceleration due to gravity.

The Association Between Alterations in Redox Homeostasis, Cortisol, and Commonly Used Objective and Subjective Markers of Fatigue in American Collegiate Football.

PURPOSE: To assess associations between a free oxygen radical test (FORT), free oxygen radical defense test (FORD), oxidative stress index, urinary cortisol, countermovement jump (CMJ), and subjective wellness in American college football. METHODS: Twenty-three male student athlete American college football players were assessed over 10 weeks: off-season conditioning (3 wk), preseason camp (4 wk), and in season (3 wk). Assessments included a once-weekly FORT and FORD blood sample, urinary cortisol sample, CMJ assessment including flight time, reactive strength index modified and concentric impulse, and a daily subjective wellness questionnaire. Linear mixed models analyzed the effect of a 2 within-subject SD change in the predictor variable on the dependent variable. The effects were interpreted using magnitude-based inference and are presented as standardized effect size (ES) ± 90% confidence intervals. RESULTS: Small negative associations were observed between FORT-flight time, FORT-fatigue, FORT-soreness (ES range = -0.30 to -0.48), FORD-sleep (ES = 0.42 ± 0.29), and oxidative stress index soreness (ES = 0.56 ± 0.29). Small positive associations were observed between FORT-cortisol (ES = 0.36 ± 0.35), FORD-flight time, FORD reactive strength index modified and FORD-soreness (0.37-0.41), oxidative stress index concentric impulse (ES = 0.37 ± 0.28), and with soreness-concentric impulse, soreness-flight time, and soreness reactive strength index modified (0.33-0.59). Moderate positive associations were observed between cortisol-concentric impulse and cortisol-sleep (0.57-0.60). CONCLUSION: FORT/FORD was associated with CMJ variables and subjective wellness. Greater amounts of subjective soreness were associated with decreased CMJ performance, increased FORT and cortisol, and decreased FORD.