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1.
Mol Ther ; 3(4): 543-50, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11319916

ABSTRACT

Gene therapy of bone would benefit from the availability of vectors that provide stable, osteoblast-specific expression. This would allow bone-specific expression of Col1a1 cDNAs for treatment of osteogenesis imperfecta. In addition, such a vector would restrict expression of secreted therapeutic proteins to the bone-synthesizing regions of the bone marrow after ex vivo transduction of marrow stromal cells and reintroduction of the cells into patients. Retrovirus vectors stably integrate into target cell genomes; however, long-term regulated expression from internal cellular promoters has not been consistently achieved. In some cases this is due to a stem cell-specific mechanism for transcriptional repression of retroviruses. We evaluated the ability of self-inactivating ROSA-derived vectors containing a bone-directed 2.3-kb rat Col1a1 promoter to display osteoblast-specific expression. In vitro expression was examined in bone marrow stromal cell cultures induced to undergo osteoblastic differentiation. In vivo expression was evaluated in chimeric mice derived from transduced embryonic stem cells. The results indicate that self-inactivating retrovirus vectors containing the Col1a1 promoter are not permanently inactivated in embryonic stem cells and are specifically expressed in osteoblasts in vivo and in vitro. Thus these vectors should be useful for bone-directed gene therapy.


Subject(s)
Bone Marrow Cells/cytology , Bone and Bones/metabolism , Collagen Type I , Collagen/genetics , Mice, Transgenic , Promoter Regions, Genetic , Retroviridae/genetics , Animals , Cell Line , Cells, Cultured , Collagen Type I, alpha 1 Chain , DNA, Complementary/metabolism , Embryo, Mammalian/cytology , Green Fluorescent Proteins , Humans , Luminescent Proteins/biosynthesis , Mice , Microscopy, Phase-Contrast , Models, Genetic , Osteoblasts/metabolism , Rats , Stem Cells/metabolism , Time Factors , Transduction, Genetic , Transfection , beta-Galactosidase/metabolism
2.
Croat Med J ; 40(4): 528-32, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10554355

ABSTRACT

AIM: To analyze the influence of the premature termination codon on mRNA transport and stability METHODS: Chondrocyte mRNA was isolated from homozygous and heterozygous nanomelic 17-days old embryos and examined by RT-PCR analysis. To analyze aggrecan mRNA stability, mRNA synthesis was inhibited with DRB [5,6 dichloro-1-(-D-ribofuranosyl benzimidazole)], a specific inhibitor of RNA polymerase II. Visualization of the aggrecan alleles was performed by in situ hybridization. RESULTS: The level of mutant aggrecan mRNA within the nucleus was equal to that of the control, but no mutant mRNA was observed in the cytoplasm. RT-PCR revealed that the mutant transcript was only detectable in the nucleus, compared with house-keeping glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene or collagen type II. A restriction site induced by premature termination codon TAA allowed the distinction of normal and mutant transcripts in chondrocytes derived from embryos heterozygous for the nanomelic mutation. After the treatment with DRB, identical decay rates were demonstrated for both transcripts within the heterozygous nucleus. In situ hybridization showed no abnormal mRNA accumulation. CONCLUSION: This is the first evidence suggesting that the transcript of the mRNA with the premature termination codon within an exon does exit the nucleus.


Subject(s)
Cartilage Diseases/genetics , Cartilage/metabolism , Chondroitin Sulfate Proteoglycans/genetics , Codon, Terminator/genetics , Extracellular Matrix Proteins , Protein Biosynthesis/genetics , Proteoglycans/genetics , RNA, Messenger/metabolism , Aggrecans , Animals , Cartilage/embryology , Cartilage Diseases/drug therapy , Cartilage Diseases/metabolism , Cell Culture Techniques , Chick Embryo , Chondrocytes/cytology , Chondrocytes/metabolism , Chondroitin Sulfate Proteoglycans/drug effects , Dichlororibofuranosylbenzimidazole/pharmacology , Genotype , Lectins, C-Type , Mutation , Nucleic Acid Synthesis Inhibitors/pharmacology , Proteoglycans/drug effects , RNA Polymerase II/antagonists & inhibitors , RNA, Messenger/drug effects , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction
3.
J Clin Invest ; 97(4): 1035-40, 1996 Feb 15.
Article in English | MEDLINE | ID: mdl-8613526

ABSTRACT

Osteogenesis imperfecta (OI) is a heritable connective tissue disorder characterized by bone fragility. Most cases of severe OI result from mutations in the coding region of the COL1A1 or COL1A2 genes yielding an abnormal collagen alpha chain. In contrast, many patients with mild OI show evidence of a null allele due to a premature stop mutation in the mutant RNA transcript. We have previously described a null allele arising from a splice donor mutation where the transcript containing the included intron was sequestered in the nucleus. Here we demonstrate that transcripts from null alleles arising from premature stop mutations are also present in the nucleus and absent in the cytoplasm. Using reverse transcriptase-PCR and single-strand conformational polymorphism of COL1A1 mRNA from patients with mild OI, we describe three patients with distinct null producing mutations identified from the mutant transcript within the nuclear compartment. A fourth patient with a Gly--->Arg expressed point mutation exhibits the mutant transcript in both compartments. Defining the distribution of allelic variants of COL1A1 mRNA in the nuclear and cytoplasmic compartments gives further insight into cell biology of OI and provides a strategy for investigating potential causes of a null allele.


Subject(s)
Collagen/genetics , Osteogenesis Imperfecta/genetics , Adult , Alleles , Base Sequence , Cell Compartmentation , Cell Nucleus/metabolism , DNA Primers/chemistry , Humans , Molecular Sequence Data , Point Mutation , Polymorphism, Single-Stranded Conformational , RNA, Messenger/genetics
4.
J Clin Invest ; 92(4): 1994-2002, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8408653

ABSTRACT

Osteogenesis imperfecta (OI) type I is the mildest form of heritable bone fragility resulting from mutations within the COL1A1 gene. We studied fibroblasts established from a child with OI type I and demonstrated underproduction of alpha 1 (I) collagen chains and alpha 1 (I) mRNA. Indirect RNase protection suggested two species of alpha 1 (I) mRNA, one of which was not collinear with fully spliced alpha 1 (I) mRNA. The noncollinear population was confined to the nuclear compartment of the cell, and contained the entire sequence of intron 26 and a G-->A transition in the first position of the intron donor site. The G-->A transition was also identified in the genomic DNA. The retained intron contained an in-frame stop codon and introduced an out-of-frame insertion within the collagen mRNA producing stop codons downstream of the insertion. These changes probably account for the failure of the mutant RNA to appear in the cytoplasm. Unlike other splice site mutations within collagen mRNA that resulted in exon skipping and a truncated but inframe RNA transcript, this mutation did not result in production of a defective collagen pro alpha 1 (I) chain. Instead, the mild nature of the disease in this case reflects failure to process the defective mRNA and thus the absence of a protein product from the mutant allele.


Subject(s)
Alternative Splicing , Collagen/genetics , Mutation , Osteogenesis Imperfecta/genetics , RNA, Messenger/genetics , Adolescent , Alleles , Amino Acid Sequence , Base Sequence , Cell Line , Collagen/biosynthesis , Exons , Female , Fibroblasts/metabolism , Humans , Introns , Molecular Sequence Data , Oligodeoxyribonucleotides , Polymerase Chain Reaction , RNA, Messenger/metabolism , Skin/metabolism
5.
J Bone Miner Res ; 7(7): 793-805, 1992 Jul.
Article in English | MEDLINE | ID: mdl-1642148

ABSTRACT

This study compares the synthesis of mutant type I collagen in cultured dermal fibroblasts and trabecular osteoblasts that were isolated from a patient with moderately severe osteogenesis imperfecta (type IV). Previous study of this patient's dermal fibroblasts revealed a 2000 dalton deletion located in cyanogen bromide peptide 4 of alpha 2(I)-collagen. The phenotype of the bone cell cultures was defined by a 3-4 day logarithmic phase doubling time, predominantly type I collagen production over type III and alkaline phosphatase activity 13.5 times dermal fibroblast levels. The current study revealed that both fibroblasts and osteoblasts synthesized a normal and a shortened alpha 2(I) chain, each as the product of separate alleles. Following pepsin treatment of the procollagens, a shortened alpha 1(I) chain was also seen in both cell types. Cyanogen bromide peptide mapping of osteoblast alpha-chains demonstrated the same deletions in the cyanogen bromide peptide 4 as observed in the fibroblast cyanogen bromide maps. PAGE analysis of oligonucleotide-specific cDNA that was reverse transcribed from RNA isolated from fibroblasts and osteoblasts also demonstrated the presence of two bands, one the normal size of alpha 2(I) cDNA and a second species that was smaller by 54 base pairs. Sequencing of polymerase chain reaction-amplified cDNA fragments revealed an in-frame deletion of exon 12. This finding was confirmed by the RNase protection method. Genomic DNA sequencing detected a T----G point mutation in the second position of the 5' splice donor site of intron 12. Therefore, in this patient with osteogenesis imperfecta there was no qualitative alteration in the osteoblast-specific expression of this mutant alpha 2(I)-collagen allele compared to dermal fibroblasts.


Subject(s)
Fibroblasts/metabolism , Osteoblasts/metabolism , Osteogenesis Imperfecta/metabolism , Procollagen/biosynthesis , Alkaline Phosphatase/metabolism , Amino Acid Sequence , Base Sequence , Cells, Cultured , Collagen/biosynthesis , DNA/analysis , Electrophoresis, Polyacrylamide Gel , Exons , Female , Humans , Middle Aged , Molecular Sequence Data , Mutation , Osteogenesis Imperfecta/genetics , Polymerase Chain Reaction , Transcription, Genetic
6.
J Clin Invest ; 89(2): 567-73, 1992 Feb.
Article in English | MEDLINE | ID: mdl-1737847

ABSTRACT

Mutations affecting the pro alpha 1(I) or pro alpha 2(I) collagen genes have been identified in each of the major clinical types of osteogenesis imperfecta. This study reports the presence of a heritable connective tissue disorder in a family with an osteopenic syndrome which has features of mild osteogenesis imperfecta but was considered idiopathic osteoporosis in the proband. At age 38, while still premenopausal, she was found to have osteopenia, short stature, hypermobile joints, mild hyperelastic skin, mild scoliosis, and blue sclerae. There was no history of vertebral or appendicular fracture. Hip and vertebral bone mineral density measurements were consistent with marked fracture risk. Delayed reduction SDS-PAGE of pepsin-digested collagens from dermal fibroblast cultures demonstrated an anomalous band migrating between alpha 1(I) and alpha 1(III). This band merged with the normal alpha-chains upon prereduction, indicating an unexpected cysteine residue. Cyanogen bromide peptide mapping suggested that the mutation was in the smaller NH2-terminal peptides. cDNA was reverse transcribed from mRNA and amplified by the polymerase chain reaction. A basepair mismatch between proband and control alpha 1(I) cDNA hybrids was detected by chemical cleavage with hydroxylamine:piperidine. The cysteine substitution was thus localized to alpha 1(I) exon 9 within the cyanogen bromide 4 peptide. Nucleotide sequence analysis localized a G----T point mutation in the first position of helical codon 43, replacing the expected glycine (GGT) residue with a cysteine (TGT). The prevalence of similar NH2-terminal mutations in subjects with this phenotype which clinically overlaps idiopathic osteoporosis remains to be determined.


Subject(s)
Collagen/genetics , Osteogenesis Imperfecta/genetics , Osteoporosis/genetics , Adolescent , Adult , Base Sequence , Child , Child, Preschool , Collagen/analysis , Cysteine/analysis , DNA/analysis , Electrophoresis, Polyacrylamide Gel , Female , Glycine/analysis , Humans , Molecular Sequence Data , Mutation , Protein Conformation
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