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2.
Invest Ophthalmol Vis Sci ; 64(14): 40, 2023 Nov 01.
Article in English | MEDLINE | ID: mdl-38015176

ABSTRACT

Purpose: Diabetic retinopathy (DR) is a leading cause of blindness in working-age adults characterized by retinal dysfunction and neurovascular degeneration. We previously reported that deletion of X-box binding protein 1 (XBP1) leads to accelerated retinal neurodegeneration in diabetes; however, the mechanisms remain elusive. The goal of this study is to determine the role of XBP1 in the regulation of photoreceptor synaptic integrity in early DR. Methods: Diabetes was induced by streptozotocin in retina-specific XBP1 conditional knockout (cKO) or wild-type (WT) mice to generate diabetic cKO (cKO/DM) or WT/DM mice for comparison with nondiabetic cKO (cKO/NDM) and WT/NDM mice. Retinal morphology, structure, and function were assessed by immunohistochemistry, optical coherence tomography, and electroretinogram (ERG) after 3 months of diabetes. The synapses between photoreceptors and bipolar cells were examined by confocal microscopy, and synaptic integrity was quantified using the QUANTOS algorithm. Results: We found a thinning of the outer nuclear layer and a decline in the b-wave amplitude in dark- and light-adapted ERG in cKO/DM mice compared to all other groups. In line with these changes, cKO mice showed increased loss of synaptic integrity compared to WT mice, regardless of diabetes status. In searching for candidate molecules responsible for the loss of photoreceptor synaptic integrity in diabetic and XBP1-deficient retinas, we found decreased mRNA and protein levels of DLG4/PSD-95 in cKO/DM retina compared to WT/DM. Conclusions: These findings suggest that XBP1 is a crucial regulator in maintaining synaptic integrity and retinal function, possibly through regulation of synaptic scaffold proteins.


Subject(s)
Diabetes Mellitus , Diabetic Retinopathy , X-Box Binding Protein 1 , Animals , Mice , Algorithms , Diabetic Retinopathy/genetics , Electroretinography , Retina , X-Box Binding Protein 1/genetics
3.
Cells ; 12(12)2023 06 06.
Article in English | MEDLINE | ID: mdl-37371028

ABSTRACT

p58IPK is a multifaceted endoplasmic reticulum (ER) chaperone and a regulator of eIF2α kinases involved in a wide range of cellular processes including protein synthesis, ER stress response, and macrophage-mediated inflammation. Systemic deletion of p58IPK leads to age-related loss of retinal ganglion cells (RGC) and exacerbates RGC damage induced by ischemia/reperfusion and increased intraocular pressure (IOP), suggesting a protective role of p58IPK in the retina. However, the mechanisms remain elusive. Herein, we investigated the cellular mechanisms underlying the neuroprotection action of p58IPK using conditional knockout (cKO) mouse lines where p58IPK is deleted in retinal neurons (Chx10-p58IPK cKO) or in myeloid cells (Lyz2-p58IPK cKO). In addition, we overexpressed p58IPK by adeno-associated virus (AAV) in the retina to examine the effect of p58IPK on RGC survival after ocular hypertension (OHT) in wild type (WT) mice. Our results show that overexpression of p58IPK by AAV significantly improved RGC survival after OHT in WT mice, suggesting a protective effect of p58IPK on reducing RGC injury. Conditional knockout of p58IPK in retinal neurons or in myeloid cells did not alter retinal structure or cellular composition. However, a significant reduction in the b wave of light-adapted electroretinogram (ERG) was observed in Chx10-p58IPK cKO mice. Deletion of p58IPK in retinal neurons exacerbates RGC loss at 14 days after OHT. In contrast, deficiency of p58IPK in myeloid cells increased the microglia/macrophage activation but had no effect on RGC loss. We conclude that deletion of p58IPK in macrophages increases their activation, but does not influence RGC survival. These results suggest that the neuroprotective action of p58IPK is mediated by its expression in retinal neurons, but not in macrophages. Therefore, targeting p58IPK specifically in retinal neurons is a promising approach for the treatment of neurodegenerative retinal diseases including glaucoma.


Subject(s)
Glaucoma , Ocular Hypertension , Animals , Mice , HSP40 Heat-Shock Proteins , Macrophage Activation , Macrophages/metabolism , Microglia/metabolism , Retinal Ganglion Cells/metabolism
4.
Mol Neurodegener ; 17(1): 25, 2022 03 28.
Article in English | MEDLINE | ID: mdl-35346303

ABSTRACT

BACKGROUND: The retina, as part of the central nervous system (CNS) with limited capacity for self-reparation and regeneration in mammals, is under cumulative environmental stress due to high-energy demands and rapid protein turnover. These stressors disrupt the cellular protein and metabolic homeostasis, which, if not alleviated, can lead to dysfunction and cell death of retinal neurons. One primary cellular stress response is the highly conserved unfolded protein response (UPR). The UPR acts through three main signaling pathways in an attempt to restore the protein homeostasis in the endoplasmic reticulum (ER) by various means, including but not limited to, reducing protein translation, increasing protein-folding capacity, and promoting misfolded protein degradation. Moreover, recent work has identified a novel function of the UPR in regulation of cellular metabolism and mitochondrial function, disturbance of which contributes to neuronal degeneration and dysfunction. The role of the UPR in retinal neurons during aging and under disease conditions in age-related macular degeneration (AMD), retinitis pigmentosa (RP), glaucoma, and diabetic retinopathy (DR) has been explored over the past two decades. Each of the disease conditions and their corresponding animal models provide distinct challenges and unique opportunities to gain a better understanding of the role of the UPR in the maintenance of retinal health and function. METHOD: We performed an extensive literature search on PubMed and Google Scholar using the following keywords: unfolded protein response, metabolism, ER stress, retinal degeneration, aging, age-related macular degeneration, retinitis pigmentosa, glaucoma, diabetic retinopathy. RESULTS AND CONCLUSION: We summarize recent advances in understanding cellular stress response, in particular the UPR, in retinal diseases, highlighting the potential roles of UPR pathways in regulation of cellular metabolism and mitochondrial function in retinal neurons. Further, we provide perspective on the promise and challenges for targeting the UPR pathways as a new therapeutic approach in age- and disease-related retinal degeneration.


Subject(s)
Retinal Degeneration , Animals , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum Stress , Mammals , Retinal Degeneration/metabolism , Signal Transduction/physiology , Unfolded Protein Response
5.
J Clin Med ; 8(6)2019 Jun 25.
Article in English | MEDLINE | ID: mdl-31242599

ABSTRACT

Retinal neuronal injury and degeneration is one of the primary manifestations of diabetic retinopathy, a leading cause of vision loss in working age adults. In pathological conditions, including diabetes and some physiological conditions such as aging, protein homeostasis can become disrupted, leading to endoplasmic reticulum (ER) stress. Severe or unmitigated ER stress can lead to cell death, which in retinal neurons results in irreversible loss of visual function. X-box binding protein 1 (XBP1) is a major transcription factor responsible for the adaptive unfolded protein response (UPR) to maintain protein homeostasis in cells undergoing ER stress. The purpose of this study is to determine the role of XBP1-mediated UPR in retinal neuronal survival and function in a mouse model of type 1 diabetes. Using a conditional retina-specific XBP1 knockout mouse line, we demonstrate that depletion of XBP1 in retinal neurons results in early onset retinal function decline, loss of retinal ganglion cells and photoreceptors, disrupted photoreceptor ribbon synapses, and Müller cell activation after induction of diabetes. Our findings suggest an important role of XBP1-mediated adaptive UPR in retinal neuronal survival and function in diabetes.

6.
Diabetologia ; 62(3): 531-543, 2019 03.
Article in English | MEDLINE | ID: mdl-30612139

ABSTRACT

AIMS/HYPOTHESIS: Müller glia (MG) are major sources of retinal cytokines, and their activation is closely linked to retinal inflammation and vascular leakage in diabetic retinopathy. Previously, we demonstrated that X-box binding protein 1 (XBP1), a transcription factor activated by endoplasmic reticulum (ER) stress in diabetic retinopathy, is involved in regulation of inflammation in retinal endothelial cells. Now, we have explored the role of XBP1 and ER stress in the regulation of MG-derived proinflammatory factors, and their influence on vascular permeability in diabetic retinopathy. METHODS: MG-specific conditional Xbp1 knockout (Xbp1Müller-/-) mice were generated by crossing Xbp1 flox/flox mice with Müller-Cre transgenic mice. Diabetes was modelled by induction with streptozotocin, and retinal vascular permeability was measured with FITC-conjugated dextran 2 months after induction. Primary Müller cells were isolated from Xbp1Müller-/- and Xbp1Müller+/+ mice and exposed to hypoxia and high levels of glucose. Levels of ER-stress and inflammatory factors were examined by real-time PCR, western blotting or immunohistochemistry. RESULTS: Xbp1Müller-/- mice exhibited normal retinal development and retinal function and expressed similar levels of ER-stress and inflammatory genes to Xbp1Müller+/+ littermates. In diabetes-inducing conditions, compared with Xbp1Müller+/+ mice, Xbp1Müller-/- mice had higher mRNA levels of retinal Vegf (also known as Vegfa) and Tnf-α (also known as Tnf) and ER-stress marker genes Grp78 (also known as Hspa5), Atf4, Chop (also known as Ddit3) and Atf6 and higher protein levels of vascular endothelial growth factor (VEGF), TNF-α, phospho-c-Jun N-terminal kinase (JNK), 78 kDa glucose-regulated protein (GRP78), phospho-eukaryotic translation initiation factor (eIF)2α and activating transcription factor (ATF)6. Retinal vascular permeability was significantly higher in diabetic Xbp1Müller-/- mice than in diabetic Xbp1Müller+/+ mice (p < 0.01). Results obtained in vitro with primary Müller cells isolated from Xbp1Müller-/- mice confirmed higher expression levels of inflammatory and ER-stress markers (but not GRP78) than in cells from Xbp1Müller+/+ mice. Moreover, XBP1-deficient Müller cells were more susceptible to high-glucose- or hypoxia-induced ER stress and inflammation than cells from Xbp1Müller+/+ mice. Inhibition of ER stress with chemical chaperones suppressed hypoxia-induced VEGF and TNF-α production in XBP1-deficient Müller cells. CONCLUSIONS/INTERPRETATION: Our results have revealed an important role of XBP1 and ER stress in MG-driven retinal inflammation, and suggest that targeting ER stress may represent a promising approach for the prevention and treatment of diabetic retinopathy.


Subject(s)
Diabetes Mellitus, Experimental/metabolism , Diabetic Retinopathy/metabolism , Ependymoglial Cells/metabolism , Inflammation/metabolism , Retina/metabolism , X-Box Binding Protein 1/metabolism , Animals , Capillary Permeability/physiology , Diabetes Mellitus, Experimental/pathology , Diabetic Retinopathy/pathology , Endoplasmic Reticulum Chaperone BiP , Endoplasmic Reticulum Stress/physiology , Ependymoglial Cells/pathology , Inflammation/pathology , Mice , Retina/pathology
7.
Front Aging Neurosci ; 10: 267, 2018.
Article in English | MEDLINE | ID: mdl-30245625

ABSTRACT

p58IPK is an endoplasmic reticulum (ER)-resident chaperone playing a critical role in facilitating protein folding and protein homeostasis. Previously, we have demonstrated that p58IPK is expressed broadly in retinal neurons including retinal ganglion cells (RGCs) and loss of p58IPK results in age-related RGC degeneration. In the present study, we investigate the role of p58IPK in neuroprotection by in vitro and in vivo studies using primary RGC culture and two well-established disease-relevant RGC injury models: retinal ischemia/reperfusion (I/R) and microbead-induced ocular hypertension. Our results demonstrate that in both in vivo models, p58IPK -/- mice exhibit significantly increased RGC loss compared to wild type (WT) mice. In vitro, p58IPK-deficient RGCs show reduced viability and are more susceptible to cell death induced by the ER stress inducer tunicamycin (TM). Overexpression of p58IPK by adeno-associated virus (AAV) significantly diminishes TM-induced cell death in both WT and p58IPK -/- RGCs. Interestingly, we find that loss of p58IPK leads to reduced mRNA expression, but not the protein level, of mesencephalic astrocyte-derived neurotrophic factor (MANF), a neurotrophic factor that resides in the ER. Treatment with recombinant MANF protein protects R28 retinal neural cells and mouse retinal explants from TM-induced cell death. Taken together, our study suggests that p58IPK functions as an endogenous neuroprotectant for RGCs. The mechanisms underlying p58IPK's neuroprotective action and the potential interactions between p58IPK and MANF warrant future investigation.

8.
Adv Exp Med Biol ; 1074: 421-427, 2018.
Article in English | MEDLINE | ID: mdl-29721972

ABSTRACT

The molecular chaperone endoplasmic reticulum protein 29 (ERp29) plays a critical role in protein folding, trafficking, and secretion. Though ubiquitously expressed, ERp29 is upregulated in response to ER stress and is found at higher levels in certain cell types such as secretory epithelial cells and neurons. As an ER resident protein, ERp29 shares many structural and functional similarities with protein disulfide isomerases, but is not regarded as part of this family due to several key differences. The broad expression and myriad roles of ERp29 coupled with its upregulation via the unfolded protein response (UPR) upon ER stress have implicated ERp29 in a range of cellular processes and diseases. We summarize the diverse activities of ERp29 in protein trafficking, cell survival and apoptosis, and ER homeostasis and highlight a potential role of ERp29 in neuroprotection in retinal and neurodegenerative diseases.


Subject(s)
Heat-Shock Proteins/physiology , Neurodegenerative Diseases/metabolism , Retinal Degeneration/metabolism , Apoptosis , DNA Repair , Endoplasmic Reticulum Stress , Epithelial Cells/metabolism , Eye Proteins/physiology , Gap Junctions/physiology , Homeostasis , Humans , Molecular Targeted Therapy , Neurodegenerative Diseases/prevention & control , Neurodegenerative Diseases/therapy , Neurons/metabolism , Protein Folding , Protein Transport , Retinal Degeneration/prevention & control , Retinal Degeneration/therapy , Unfolded Protein Response
9.
Mol Neurodegener ; 13(1): 16, 2018 04 04.
Article in English | MEDLINE | ID: mdl-29615095

ABSTRACT

BACKGROUND: Aging is the strongest risk factor for neurodegenerative diseases and extended age results in neuronal degeneration and functional decline in the visual system. Among many contributing factors to age-related deterioration of neurons is an insufficient activation of the Unfolded Protein Response (UPR) in the endoplasmic reticulum (ER) in response to cellular stress. X-box binding protein 1 (XBP1) is a major component of the UPR and is essential for maintaining protein homeostasis and reducing cellular stresses. Herein, we investigate the role of XBP1 in maintaining morphological and functional integrity in retinal neurons during adulthood and the early stages of aging. METHODS: The basal and induced levels of XBP1 activation in the retina were measured in young adult and aged mice. Conditional knockout (cKO) of XBP1 in retinal neurons was achieved by crossing XBP1 floxed mice with a retina specific Cre-recombinase line (Chx10-Cre). Retinal morphology, neuronal populations including photoreceptors, bipolar cells, and retinal ganglion cells (RGCs), synaptic structure, and microglial activation were examined with immunohistochemistry and staining of retinal sections. Retinal function was evaluated with light-adapted (photopic) and dark adapted (scotopic) electroretinograms. Retinal mitochondrial function and metabolism was assessed by Seahorse XFe24 Extracellular Flux Analyzer. RESULTS: The retinas of aged wild type (WT) mice display a significantly reduced basal level of Xbp1s and compromised activation of ER stress response. In XBP1 cKO mice, significant structural degeneration of the retina, evidenced by thinning of retinal layers and a loss of RGCs, and functional defects indicated by diminished photopic and scotopic ERG b-waves are observed at the age of 12-14 months. Furthermore, discontinuous and disorganized synaptic laminae, colocalized with activated microglia, in the inner plexiform layer is found in the XBP1 cKO retinas. In addition, cKO mice demonstrate a significant increase in ectopic synapses between bipolar cells and photoreceptors, which is strikingly similar to WT mice at 20-24 months of age. These changes are associated with defective retinal glycolysis while mitochondrial respiratory function appears normal in the cKO retina. CONCLUSIONS: XBP1 cKO mice at 12-14 months of age show significant structural, functional, and metabolic deficits that closely resemble WT mice twice that age. Our findings suggest that the absence of XBP1, a critical component of the UPR, accelerates age-related retinal neurodegeneration.


Subject(s)
Aging/metabolism , Nerve Degeneration/metabolism , Retina/metabolism , Unfolded Protein Response/physiology , X-Box Binding Protein 1/metabolism , Aging/pathology , Animals , Mice , Mice, Knockout , Nerve Degeneration/pathology , Retina/pathology
10.
Neural Regen Res ; 10(8): 1211-3, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26487839
11.
Mol Cell Neurosci ; 63: 24-30, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25051176

ABSTRACT

The projection from the retina to the superior colliculus in mice is organized in a retinotopic map that develops through the formation and guidance of interstitial branches extended by retinal ganglion cell axons. Bidirectional branch guidance along the lateral-medial collicular axis is critical to mapping the dorsal-ventral retinal axis. EphB receptor tyrosine kinases expressed in an overall low to high dorsal-ventral retinal gradient have been implicated in this mapping in response to the graded low to high lateral-medial expression of a ligand, ephrin-B1, in the superior colliculus. However, the relative contributions of EphBs and ephrin-B1 are not well understood. We examined EphB1, EphB2, and EphB3 mutant mice and find that each has ectopic arborizations of retinal axon branches lateral to their appropriate termination zone, with no qualitative differences in aberrant mapping, suggesting a similar role for each EphB. However, the frequency of cases with map defects progressively rises in compound EphB mutants coincident with the number of EphB null alleles from one to five of the six total alleles indicating that EphB level is critical. We analyzed branch extension in vitro and find that dorsal branches, with low EphB levels, exhibit a negative response to ephrin-B1, whereas ventral branches, with high EphB levels, exhibit a positive response to ephrin-B1. Using EphB mutant retina, we show that both of these differential branch extension responses are dependent on EphB level. Our findings show a bifunctional action of ephrin-B1 regulated by EphB levels that can account for the bidirectional extension of interstitial branches required to establish a retinotopic map.


Subject(s)
Ephrin-B1/metabolism , Receptors, Eph Family/metabolism , Retina/metabolism , Animals , Axons/metabolism , Cells, Cultured , Mice , Mutation , Receptors, Eph Family/genetics , Retina/cytology , Retina/physiology , Superior Colliculi/cytology , Superior Colliculi/metabolism , Superior Colliculi/physiology
12.
J Neurosci ; 34(19): 6438-47, 2014 May 07.
Article in English | MEDLINE | ID: mdl-24806670

ABSTRACT

In the developing brain, initial neuronal projections are formed through extensive growth and branching of developing axons, but many branches are later pruned to sculpt the mature pattern of connections. Despite its widespread occurrence, the mechanisms controlling pruning remain incompletely characterized. Based on pharmacological and biochemical analysis in vitro and initial genetic analysis in vivo, prior studies implicated a pathway involving binding of the Amyloid Precursor Protein (APP) to Death Receptor 6 (DR6) and activation of a downstream caspase cascade in axonal pruning. Here, we further test their involvement in pruning in vivo and their mechanism of action through extensive genetic and biochemical analysis. Genetic deletion of DR6 was previously shown to impair pruning of retinal axons in vivo. We show that genetic deletion of APP similarly impairs pruning of retinal axons in vivo and provide evidence that APP and DR6 act cell autonomously and in the same pathway to control pruning. Prior analysis had suggested that ß-secretase cleavage of APP and binding of an N-terminal fragment of APP to DR6 is required for their actions, but further genetic and biochemical analysis reveals that ß-secretase activity is not required and that high-affinity binding to DR6 requires a more C-terminal portion of the APP ectodomain. These results provide direct support for the model that APP and DR6 function cell autonomously and in the same pathway to control pruning in vivo and raise the possibility of alternate mechanisms for how APP and DR6 control pruning.


Subject(s)
Amyloid Precursor Protein Secretases/physiology , Amyloid beta-Protein Precursor/genetics , Axons/physiology , Receptors, Tumor Necrosis Factor/genetics , Signal Transduction/physiology , Animals , Animals, Genetically Modified , Cell Count , Cells, Cultured , Ganglia, Spinal/cytology , Ganglia, Spinal/physiology , Immunohistochemistry , Immunoprecipitation , Mice , Nerve Degeneration/genetics , Nerve Degeneration/pathology , Protein Binding , RNA, Small Interfering/genetics , Retinal Ganglion Cells/physiology , Sensory Receptor Cells/physiology
13.
J Neurosci ; 32(49): 17540-53, 2012 Dec 05.
Article in English | MEDLINE | ID: mdl-23223278

ABSTRACT

Axon degeneration initiated by trophic factor withdrawal shares many features with programmed cell death, but many prior studies discounted a role for caspases in this process, particularly Caspase-3. Recently, Caspase-6 was implicated based on pharmacological and knockdown evidence, and we report here that genetic deletion of Caspase-6 indeed provides partial protection from degeneration. However, we find at a biochemical level that Caspase-6 is activated effectively only by Caspase-3 but not other "upstream" caspases, prompting us to revisit the role of Caspase-3. In vitro, we show that genetic deletion of Caspase-3 is fully protective against sensory axon degeneration initiated by trophic factor withdrawal, but not injury-induced Wallerian degeneration, and we define a biochemical cascade from prosurvival Bcl2 family regulators to Caspase-9, then Caspase-3, and then Caspase-6. Only low levels of active Caspase-3 appear to be required, helping explain why its critical role has been obscured in prior studies. In vivo, Caspase-3 and Caspase-6-knockout mice show a delay in developmental pruning of retinocollicular axons, thereby implicating both Caspase-3 and Caspase-6 in axon degeneration that occurs as a part of normal development.


Subject(s)
Axons/enzymology , Caspase 3/physiology , Caspase 6/physiology , Nerve Degeneration/enzymology , Superior Colliculi/growth & development , Animals , Axons/pathology , Axons/ultrastructure , Caspase 3/genetics , Caspase 6/genetics , Cells, Cultured , Enzyme Activation/physiology , Mice , Mice, Inbred C57BL , Mice, Knockout , Molecular Imaging/methods , Nerve Degeneration/genetics , Nerve Degeneration/pathology , Nerve Growth Factor/adverse effects , Proto-Oncogene Proteins c-bcl-2/physiology , Sensory Receptor Cells/enzymology , Sensory Receptor Cells/pathology , Signal Transduction/genetics , Signal Transduction/physiology , Superior Colliculi/enzymology , Wallerian Degeneration/enzymology , Wallerian Degeneration/genetics , Wallerian Degeneration/pathology , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/physiology
14.
J Neurochem ; 121(2): 263-76, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22303986

ABSTRACT

Insulin offers trophic support through receptors expressed widely on peripheral neurons. In this work, we studied whether peripheral sensory neurons demonstrate resistance to its trophic properties, a property relevant during type 2 diabetes mellitus or following supraphysiological therapy. Insulin receptors were not only localized to neuronal membranes and cytoplasm but also had a unique, previously unrecognized localization to neuronal nuclei. We confirmed that nanomolar doses increased neurite outgrowth of adult sensory neurons, but in response to micromolar doses of insulin, even following a brief 2-h exposure, survival and outgrowth of neurites were blunted. Neurons exposed to picomolar insulin concentrations in their media for 5 days had resistance to the impact of later nanomolar doses of insulin. Using a stripe assay seeded with insulin, neurites were more likely to reject higher doses of insulin. Insulin down-regulated mRNAs of the insulin receptor ß subunit and up-regulated levels of GSK-3ß, both potential mechanisms of insulin resistance, while down-regulating the protein expression of pAkt and pGSK-3ß. Overall, these studies identify neuronal nuclear targeting of insulin and evidence for insulin-induced resistance to its trophic properties. The findings have implications for the understanding of the actions of insulin in the treatment of diabetes and neurological disorders.


Subject(s)
Hypoglycemic Agents/pharmacology , Insulin/pharmacology , Neurites/drug effects , Sensory Receptor Cells/drug effects , Animals , Axons/physiology , Blotting, Western , Cell Membrane/drug effects , Cell Nucleus/drug effects , Cells, Cultured , Cytoplasm/drug effects , Female , Gene Expression/drug effects , Glycogen Synthase Kinase 3/biosynthesis , Glycogen Synthase Kinase 3 beta , Immunohistochemistry , Insulin Resistance/genetics , Insulin Resistance/physiology , Male , Mice , Mice, Inbred C57BL , Oncogene Protein v-akt/biosynthesis , Rats , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction , Receptor, Insulin/biosynthesis
15.
Nature ; 457(7232): 981-9, 2009 Feb 19.
Article in English | MEDLINE | ID: mdl-19225519

ABSTRACT

Naturally occurring axonal pruning and neuronal cell death help to sculpt neuronal connections during development, but their mechanistic basis remains poorly understood. Here we report that beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. DR6 is broadly expressed by developing neurons, and is required for normal cell body death and axonal pruning both in vivo and after trophic-factor deprivation in vitro. Unlike neuronal cell body apoptosis, which requires caspase 3, we show that axonal degeneration requires caspase 6, which is activated in a punctate pattern that parallels the pattern of axonal fragmentation. DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation, and we identify APP as a DR6 ligand. Trophic-factor deprivation triggers the shedding of surface APP in a beta-secretase (BACE)-dependent manner. Loss- and gain-of-function studies support a model in which a cleaved amino-terminal fragment of APP (N-APP) binds DR6 and triggers degeneration. Genetic support is provided by a common neuromuscular junction phenotype in mutant mice. Our results indicate that APP and DR6 are components of a neuronal self-destruction pathway, and suggest that an extracellular fragment of APP, acting via DR6 and caspase 6, contributes to Alzheimer's disease.


Subject(s)
Amyloid beta-Protein Precursor/metabolism , Axons/metabolism , Caspase 6/metabolism , Caspases/metabolism , Neurons/cytology , Neurons/metabolism , Receptors, Tumor Necrosis Factor/metabolism , Alzheimer Disease/metabolism , Amyloid beta-Protein Precursor/chemistry , Animals , Caspase 3/metabolism , Cell Death , Ligands , Mice , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Protein Binding , Signal Transduction , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism
16.
Neuron ; 59(5): 746-58, 2008 Sep 11.
Article in English | MEDLINE | ID: mdl-18786358

ABSTRACT

Reverse signaling by ephrin-As upon binding EphAs controls axon guidance and mapping. Ephrin-As are GPI-anchored to the membrane, requiring that they complex with transmembrane proteins that transduce their signals. We show that the p75 neurotrophin receptor (NTR) serves this role in retinal axons. p75(NTR) and ephrin-A colocalize within caveolae along retinal axons and form a complex required for Fyn phosphorylation upon binding EphAs, activating a signaling pathway leading to cytoskeletal changes. In vitro, retinal axon repulsion to EphAs by ephrin-A reverse signaling requires p75(NTR), but repulsion to ephrin-As by EphA forward signaling does not. Constitutive and retina-specific p75(NTR) knockout mice have aberrant anterior shifts in retinal axon terminations in superior colliculus, consistent with diminished repellent activity mediated by graded ephrin-A reverse signaling induced by graded collicular EphAs. We conclude that p75(NTR) is a signaling partner for ephrin-As and the ephrin-A- p75(NTR) complex reverse signals to mediate axon repulsion required for guidance and mapping.


Subject(s)
Axons/physiology , Brain Mapping , Ephrins/metabolism , Retinal Ganglion Cells/cytology , Signal Transduction/physiology , Amino Acids/metabolism , Animals , Animals, Newborn , Cell Line, Transformed , GAP-43 Protein/genetics , GAP-43 Protein/metabolism , Gene Expression Regulation/physiology , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Humans , Mice , Mice, Transgenic , Mutation , Nerve Tissue Proteins , Proto-Oncogene Proteins c-fyn/metabolism , Rats , Receptors, Nerve Growth Factor , Retina/cytology , Retinal Ganglion Cells/physiology , Visual Pathways/physiology
17.
Neuron ; 50(6): 883-95, 2006 Jun 15.
Article in English | MEDLINE | ID: mdl-16772170

ABSTRACT

Axon pruning by degeneration remodels exuberant axonal connections and is widely required for the development of proper circuitry in the nervous system from insects to mammals. Developmental axon degeneration morphologically resembles injury-induced Wallerian degeneration, suggesting similar underlying mechanisms. As previously reported for mice, we show that Wlds protein substantially delays Wallerian degeneration in flies. Surprisingly, Wlds has no effect on naturally occurring developmental axon degeneration in flies or mice, although it protects against injury-induced degeneration of the same axons at the same developmental age. By contrast, the ubiquitin-proteasome system is intrinsically required for both developmental and injury-induced axon degeneration. We also show that the glial cell surface receptor Draper is required for efficient clearance of axon fragments during developmental axon degeneration, similar to its function in injury-induced degeneration. Thus, mechanistically, naturally occurring developmental axon pruning by degeneration and injury-induced axon degeneration differ significantly in early steps, but may converge onto a common execution pathway.


Subject(s)
Axons/physiology , Drosophila Proteins/physiology , Gene Expression Regulation, Developmental/physiology , Nerve Tissue Proteins/physiology , Wallerian Degeneration/metabolism , Animals , Animals, Genetically Modified , Axons/metabolism , Axons/ultrastructure , Drosophila , Drosophila Proteins/biosynthesis , Drosophila Proteins/genetics , Mice , Mice, Inbred C57BL , Nerve Tissue Proteins/biosynthesis , Nerve Tissue Proteins/genetics , Wallerian Degeneration/genetics , Wallerian Degeneration/pathology
18.
Annu Rev Neurosci ; 28: 327-55, 2005.
Article in English | MEDLINE | ID: mdl-16022599

ABSTRACT

Gradients of axon guidance molecules have long been postulated to control the development of the organization of neural connections into topographic maps. We review progress in identifying molecules required for mapping and the mechanisms by which they act, focusing on the visual system, the predominant model for map development. The Eph family of receptor tyrosine kinases and their ligands, the ephrins, remain the only molecules that meet all criteria for graded topographic guidance molecules, although others fulfill some criteria. Recent reports further define their modes of action and new roles for them, including EphB/ephrin-B control of dorsal-ventral mapping, bidirectional signaling of EphAs/ephrin-As, bifunctional action of ephrins as attractants or repellents in a context-dependent manner, and complex interactions between multiple guidance molecules. In addition, spontaneous patterned neural activity has recently been shown to be required for map refinement during a brief critical period. We speculate on additional activities required for map development and suggest a synthesis of molecular and cellular mechanisms within the context of the complexities of map development.


Subject(s)
Brain Mapping , Gene Expression Regulation, Developmental/physiology , Retina/embryology , Retinal Ganglion Cells/physiology , Visual Pathways/embryology , Animals , Axons/physiology , Embryonic Development , Humans , Models, Biological , Receptors, Eph Family/genetics , Receptors, Eph Family/metabolism , Retina/cytology , Retinal Ganglion Cells/cytology
19.
Prog Brain Res ; 147: 43-65, 2005.
Article in English | MEDLINE | ID: mdl-15581697

ABSTRACT

This chapter summarizes mechanisms that control the development of retinotopic maps in the brain, focusing on work from our laboratory using as models the projection of retinal ganglion cells (RGCs) to the chick optic tectum (OT) or rodent superior colliculus (SC). The formation of a retinotopic map involves the establishment of an initial, very coarse map that subsequently undergoes large-scale remodeling to generate a refined map. All arbors are formed by interstitial branches that form in a topographically biased manner along RGC axons that overshoot their correct termination zone (TZ) along the anterior-posterior (A-P) axis of the OT/SC. The interstitial branches exhibit directed growth along the lateral-medial (L-M) axis of the OT/SC to position the branch at the topographically correct location, where it arborizes to form the TZ. EphA receptors and ephrin-A ligands control in part RGC axon mapping along the A-P axis by inhibiting branching and arborization posterior to the correct TZ. Ephrin-B1 acts bifunctionally through EphB forward signaling to direct branches along the L-M axis of the OT/SC to their topographically correct site. Computational modeling indicates that multiple graded activities are required along each axis to generate a retinotopic map, and makes several predictions, including: the progressive addition of ephrin-As within the OT/SC, due to its expression on RGC axon branches and arbors, is required to increase topographic specificity in branching and arborization as well as eliminate the initial axon overshoot, and that interactions amongst RGC axons that resemble correlated neural activity are required to drive retinotopic refinement. Analyses of mutant mice that lack early spontaneous retinal waves that correlate activity amongst neighboring RGCs, confirm this modeling prediction and show that correlated activity during an early brief critical period is required to drive the large-scale remodeling of the initially topographically coarse projection into a refined one. In summary, multiple graded guidance molecules, retinal waves and correlated spontaneous RGC activity cooperate to generate retinotopic maps.


Subject(s)
Aging/physiology , Brain/embryology , Brain/growth & development , Retina/embryology , Retina/growth & development , Visual Pathways/embryology , Visual Pathways/growth & development , Animals , Embryonic Development , Ephrins/metabolism , Receptors, Eph Family/metabolism , Retina/physiology
20.
J Neurobiol ; 59(1): 95-113, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15007830

ABSTRACT

The topographic projection of retinal ganglion cell (RGC) axons to mouse superior colliculus (SC) or chick optic tectum (OT) is formed in three phases: RGC axons overshoot their termination zone (TZ); they exhibit interstitial branching along the axon that is topographically biased for the correct location of their future TZ; and branches arborize preferentially at the TZ and the initial exuberant projection refines through axon and branch elimination to generate a precise retinotopic map. We present a computational model of map development that demonstrates that the countergradients of EphAs and ephrinAs in retina and the OT/SC and bidirectional repellent signaling between RGC axons and OT/SC cells are sufficient to direct an initial topographic bias in RGC axon branching. Our model also suggests that a proposed repellent action of EphAs/ephrinAs present on RGC branches and arbors added to that of EphAs/ephrinAs expressed by OT/SC cells is required to progressively restrict branching and arborization to topographically correct locations and eliminate axon overshoot. Simulations show that this molecular framework alone can develop considerable topographic order and refinement, including axon elimination, a feature not programmed into the model. Generating a refined map with a condensed TZ as in vivo requires an additional parameter that enhances branch formation along an RGC axon near sites that it has a higher branch density, and resembles an assumed role for patterned neural activity. The same computational model generates the phenotypes reported in ephrinA deficient mice and Isl2-EphA3 knockin mice. This modeling suggests that gradients of counter-repellents can establish a substantial degree of topographic order in the OT/SC, and that repellents present on RGC axon branches and arbors make a substantial contribution to map refinement. However, competitive interactions between RGC axons that enhance the probability of continued local branching are required to generate precise retinotopy.


Subject(s)
Axons/physiology , Body Patterning/physiology , Neural Networks, Computer , Receptors, Eph Family/physiology , Retina/physiology , Animals , Brain Mapping , Chick Embryo , Computer Simulation , Gene Expression Regulation, Developmental , Mice , Mice, Knockout , Retina/cytology , Retinal Ganglion Cells/cytology , Retinal Ganglion Cells/physiology , Superior Colliculi
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