Evaluation of the reliability of the Cutaneous Dermatomyositis Disease Area and Severity Index and the Cutaneous Assessment Tool-Binary Method in juvenile dermatomyositis among paediatric dermatologists, rheumatologists and neurologists.

BACKGROUND: The Cutaneous Dermatomyositis Disease Area and Severity Index (CDASI) and Cutaneous Assessment Tool-Binary Method (CAT-BM) have been shown to be reliable and valid outcome measures to assess cutaneous disease in adult dermatomyositis (DM) and juvenile DM (JDM), respectively. OBJECTIVES: This study compared the CDASI and CAT-BM for use by paediatric dermatologists, paediatric rheumatologists and paediatric neurologists in patients with JDM. METHODS: Five paediatric dermatologists, five paediatric rheumatologists and five paediatric neurologists each evaluated 14 patients with JDM using the CDASI, CAT-BM, and skin Physician Global Assessment (PGA) scales. Inter-rater reliability, intra-rater reliability, construct validity and completion time were compared. RESULTS: Inter-rater reliability for CDASI activity and damage scores was good to moderate for paediatric dermatologists and rheumatologists, but poor for paediatric neurologists. The inter-rater reliability for CAT-BM activity scores was moderate for paediatric dermatologists and rheumatologists, but poor for paediatric neurologists and poor across all specialties for damage scores. Intra-rater reliability for the CDASI and CAT-BM activity and damage scores was moderate to excellent for paediatric dermatologists, rheumatologists and neurologists. Strong associations were found between skin PGA activity and damage scores and CDASI or CAT-BM activity and damage scores, respectively (P < 0·002). The CDASI had a mean completion time of 5·4 min compared with that for the CAT-BM of 3·1 min. CONCLUSIONS: Our data confirm the reliability of the CDASI activity and damage scores and the CAT-BM activity scores when used by paediatric dermatologists and rheumatologists in assessing JDM. Significant variation existed in the paediatric neurologists' scores.

The relationship between the orientation of the glenoid and tears of the rotator cuff.

Our aim was to determine the most repeatable three-dimensional measurement of glenoid orientation and to compare it between shoulders with intact and torn rotator cuffs. Our null hypothesis was that glenoid orientation in the scapulae of shoulders with a full-thickness tear of the rotator cuff was the same as that in shoulders with an intact rotator cuff. We studied 24 shoulders in cadavers, 12 with an intact rotator cuff and 12 with a full-thickness tear. Two different observers used a three-dimensional digitising system to measure glenoid orientation in the scapular plane (ie glenoid inclination) using six different techniques. Glenoid version was also measured. The overall precision of the measurements revealed an error of less than 0.6 degrees. Intraobserver reliability (correlation coefficients of 0.990 and 0.984 for each observer) and interobserver reliability (correlation coefficient of 0.985) were highest for measurement of glenoid inclination based on the angle obtained from a line connecting the superior and inferior points of the glenoid and that connecting the most superior point of the glenoid and the most superior point on the body of the scapula. There were no differences in glenoid inclination (p = 0.34) or glenoid version (p = 0.12) in scapulae from shoulders with an intact rotator cuff and those with a full-thickness tear. Abnormal glenoid orientation was not present in shoulders with a torn rotator cuff.

Thermal and cold neutron phase-contrast radiography.

In this paper, we will discuss a phase-contrast imaging method that avoids the complications of interferometry to provide phase contrast in weakly absorbing samples. A transversely coherent neutron beam is used with the traditional radiography scheme. Images taken with this scheme show dramatic intensity variations due to sharp changes in the neutron wave refractive index. With some numerical processing these images may be used to reconstruct a quantitative phase radiograph of specimens imaged with this technique.

Quantitative phase-amplitude microscopy. III. The effects of noise.

We explore the effect of noise on images obtained using quantitative phase-amplitude microscopy - a new microscopy technique based on the determination of phase from the intensity evolution of propagating radiation. We compare the predictions with experimental results and also propose an approach that allows good-quality quantitative phase retrieval to be obtained even for very noisy data.

Quantitative phase radiography with polychromatic neutrons.

We develop and experimentally demonstrate a formalism that allows accurate phase imaging using neutron sources producing highly polychromatic beams. The results of measurements from a rectangular block of silicon compare favorably with theoretical simulations based upon the known composition and geometry of the block. The increased flux and reduced exposure times will permit a simple extension of the technique to tomographic phase imaging.

Measurement of the spatial coherence function of undulator radiation using a phase mask.

A measurement of the horizontal coherence function of 7.9 keV radiation from an undulator beam line at the Advanced Photon Source is reported. X-ray diffraction from a phase-shifting mask was used, and the coherence function was measured as a function of the width of beam-conditioning slits in the beam line. The coherence distribution is found to be best described by a Lorentzian function.

Quantitative phase-amplitude microscopy II: differential interference contrast imaging for biological TEM.

Although phase contrast microscopy is widespread in optical microscopy, it has not been as widely adopted in transmission electron microscopy (TEM), which has therefore to a large extent relied on staining techniques to yield sufficient contrast. Those methods of phase contrast that are used in biological electron microscopy have been limited by factors such as the need for small phase shifts in very thin samples, the requirement for difficult experimental conditions, or the use of complex data analysis methods. We here demonstrate a simple method for quantitative TEM phase microscopy that is suitable for large phase shifts and requires only two images. We present a TEM phase image of unstained Radula sp. (liverwort spore). We show how the image may be transformed into the differential interference contrast image format familiar from optical microscopy. The phase images contain features not visible with the other imaging modalities. The resulting technique should permit phase contrast TEM to be performed almost as readily as phase contrast optical microscopy.

The pore structure in processed Victorian Brown coal.

Changes in the pore structure of Victorian Brown coal when upgraded with heated gases under pressure are investigated. We show that the results obtained from ultra-small-angle neutron scattering (USANS) measurements agree with previous results using small-angle X-ray scattering results but that USANS may also be used to investigate the meso porosity. Findings from small-angle scattering are confirmed using electron microscopy. We also show evidence from electron diffraction that thermal conditions within the brown coals during the upgrade procedure may be far more extreme than previously thought.

The effects of tibial rotation on the patellofemoral joint: assessment of the changes in in situ strain in the peripatellar retinaculum and the patellofemoral contact pressures and areas.

The objective of this study was to determine the effects of tibial rotation on in situ strain in the peripatellar retinaculum and patellofemoral contact pressures and areas. Patellofemoral joint biomechanics demonstrate a strong correlation with the etiology of patellofemoral disorders, such as chondromalacia, and are significantly influenced by tibial rotation. Six human cadaveric knees were used along with a patellofemoral joint testing jig that permits physiological loading of the knee extensor muscles. Patellofemoral contact pressures and areas were measured with a Fuji pressure-sensitive film, and the changes in in situ strain in the peripatellar retinaculum were measured with four differential variable reluctance transducers. Tibial rotation had a significant effect on patellofemoral joint biomechanics. The data showed an inverse relationship between increasing knee flexion angles and the change in patellofemoral contact pressures and in situ strain with tibial rotation. At higher knee flexion angles, the patella is well-seated in the trochlear groove and the function of the peripatellar retinaculum is minimized and less affected by tibial rotations.

Deformation and strain characteristics along the length of the anterior band of the inferior glenohumeral ligament.

Efficacious surgical treatment of anterior glenohumeral instability often requires repair of the anteroinferior capsulolabral structures, including the glenoid origin of the anterior band of the inferior glenohumeral ligament. Rupture in this location, the Bankart lesion, may be accompanied by nonrecoverable stretching of the anterior band. The purpose of this study was to evaluate the amount and location of nonrecoverable stretching with tensile testing. Twelve glenoid-soft tissue-humerus complexes from fresh-frozen glenohumeral joints were studied by means of a custom jig, an Instron machine, and a video digitizing system. The joints were positioned to simulate that known to cause apprehension for anterior instability. Nonrecoverable deformation differed along the length of the anterior band but was slight in all locations. For those that failed at the glenoid insertion region, the mean nonrecoverable deformation was 0.10 +/- 0.16 mm (mean +/- SEM) at the bone-labral junction of the glenoid insertion region and 0.38 +/- 0.23 mm at the labral-ligament junction of the glenoid insertion region. It was 0.53 +/- 0.23 mm at the ligament midsubstance and 0.04 +/- 0.10 mm at the humeral insertion region. For those that failed at the glenoid insertion region, the nonrecoverable stretching was 1.4% +/- 1.9% at the bone-labral junction of the glenoid insertion region and 3.5% +/- 2.0% at the labral-ligament junction of the glenoid insertion region. It was 2.3% +/- 1.1% at the ligament midsubstance and 0.0% +/- 1.4% at the humeral insertion region. Rupture of the anterior band resulted in little nonrecoverable stretching at both the site of failure and elsewhere along the length, remote from the failure site. Surgical repairs after initial dislocation may restore the length of the anterior band of the inferior glenohumeral ligament with little shortening.

The healing effects on the biomechanical properties of joint capsular tissue treated with Ho:YAG laser: An in vivo rabbit study.

PURPOSE: The objective of this study was to evaluate the healing response, after thermal treatment with a Ho:YAG laser, on the biomechanical properties of capsular soft tissue. TYPE OF STUDY: Before and after trial. METHODS: Forty-five New Zealand white rabbits were used in this study. A medial peripatellar retinacular thermal capsuloplasty using a Ho:YAG laser and a lateral peripatellar retinacular release was performed on 1 knee of each rabbit. The contralateral knee served as a control and had a lateral release of the retinaculum only. The temperature of the medial retinaculum was maintained at 55 degrees C +/- 5 degrees C during treatment. The medial peripatellar retinaculum was evaluated at 0, 6, and 12 weeks postoperatively. Tensile testing of the medial retinaculum and a biomechanical assessment evaluating the structural and material properties were performed. RESULTS: The ultimate load (force) of the medial retinaculum was 70%, 56%, and 84% of control at 0, 6, and 12 weeks, respectively, after the procedure. The stiffness (force/deformation) of the medial retinaculum was 83% of control at 0 weeks, 54% at 6 weeks, and 85% at 12 weeks. The ultimate stress (force/area) of the medial retinaculum also showed a significant reduction at 0 and 6 weeks postoperatively, 63% and 62% of control, respectively. By 12 weeks, the ultimate stress was 83% of control. CONCLUSIONS: Thermal treatment of the medial retinaculum with a Ho:YAG laser results in soft tissue with significantly diminished biomechanical properties after treatment. The results of this study suggest that a 12-week period of minimal stress on the capsular tissues should follow a thermal capsuloplasty procedure.

Arthroscopic suture tying: A comparison of knot types and suture materials.

PURPOSE: The purpose of this study was to determine the structural properties of 3 arthroscopically tied knots using 2 different suture materials: the French knot, the Duncan loop knot, and the original Revo knot. TYPE OF STUDY: Cohort analytic study. METHODS: The sutures used were No. 1 PDS II, an absorbent monofilament, and No. 1 Ethibond (Ethicon, Somerville, NJ), a braided nonabsorbent material. The resulting 6 suture-knot combinations were individually tested to failure in both open- and closed-loop configurations. RESULTS: The French knot showed the greatest strength compared with the Duncan loop and the Revo knot with both No. 1 Ethibond and No. 1 PDS II sutures (P <.05). The No. 1 Ethibond exhibited higher initial stiffness than the No. 1 PDS II for all 3 knot types (P <.05). Results were similar for both open and closed-loop configurations. Also, the French knot failed predominantly by suture breakage instead of knot slippage for both suture materials. CONCLUSIONS: The results of this study suggest that, among the suture and knot combinations investigated in this study, the arthroscopic repair of musculoskeletal injuries should be performed using the French knot and No. 1 Ethibond suture.

Release of the coracoacromial ligament can lead to glenohumeral laxity: a biomechanical study.

The purpose of this study was to determine change in glenohumeral joint translation after release of the coracoacromial ligament. Six fresh, frozen unpaired glenohumeral joints were tested in a neutral position and at 30 degrees internal and 30 degrees external rotation of the humerus at 0 degrees, 30 degrees, and 60 degrees of abduction on a custom glenohumeral joint translation testing apparatus. A joint compression load of 20 N was simulated; then a 15-N load was applied to the humerus in anterior, posterior, superior, and inferior directions, and translations on the glenoid were measured with an electromagnetic tracking device. The tests were then repeated after a 1.5-cm section of the coracoacromial ligament was released from the acromion. A multivariate analysis of variance was used for statistical analyses with a P value of.05 as the level of significance. At 0 degrees and 30 degrees of abduction, release of the coracoacromial ligament resulted in a significant increase in glenohumeral joint translations, in both the anterior and inferior directions. In addition, the differences in translation between before and after the release of the coracoacromial ligament decreased in all directions as glenohumeral abduction increased, and they were not significant at 60 degrees of abduction in any of the rotations. The results of this study suggest that the coracoacromial ligament has a role in static restraint of the glenohumeral joint. It provides a suspension function and may restrain anterior and inferior translations through an interaction with the coracohumeral ligament. Although this is a biomechanical study without simulation of the shoulder muscles, it indicates that the coracoacromial ligament contributes to glenohumeral stability. Caution should be exercised in the release of the coracoacromial ligament in those with rotator cuff pain associated with glenohumeral instability.

Precision of ACL tunnel placement using traditional and robotic techniques.

The objective of this study was to examine the precision of ACL tunnel placement using: (1) CASPAR (orto MAQUET GmbH Co. KG)--an active robotic system, and (2) four orthopedic surgeons with various levels of experience (between 100 and 3,500 ACL reconstructions). The robotic system and each surgeon drilled tunnels for ACL reconstruction in 10 plastic knees (total n = 50) that included a reference cube in the medial aspect of the proximal tibia and distal femur. For the robotic system, the placement of each tunnel was planned preoperatively using custom software and CT data for each femur and tibia. The robotic system then drilled the tunnels in the femur and tibia based on the preoperative plan. For the surgeons, tunnel placement was accomplished using their preferred technique, which was based on the one-incision arthroscopic technique. The distribution of intra-articular points on the tibia was contained within a sphere of radius 2.0 mm (robot system), 2.1 mm (Fellow 1), 2.4 mm (Fellow 2), 3.4 mm (Experienced Surgeon 1), or 2.0 mm (Experienced Surgeon 2). On the femur, no significant differences in the distribution of intra-articular points could be demonstrated between the robotic system (2.1 mm), Fellow 1 (4.5 mm), Fellow 2 (4.1 mm), Experienced Surgeon 1 (2.3 mm), and Experienced Surgeon 2 (3.0 mm). The direction of the tunnels drilled in the femur and tibia was different with the robotic and traditional techniques. However, the robotic system had the most consistent tunnel directions, while the surgeons' tunnels were more dispersed. Variation in surgeon precision of tunnel placement for ACL reconstruction is greater on the femur than the tibia, and this can be correlated with experience. Our data also suggest that the robotic system has the same precision as the most experienced surgeons.

The effects of ulnar axial malalignment on supination and pronation.

BACKGROUND: Forearm fractures are common injuries in both adults and children. Despite efforts to obtain anatomical alignment, axial rotational malunions occur, resulting in a decreased range of motion and a poor appearance. The objective of this study was to quantify loss of forearm rotation after simulation of ulnar malunions in supination and pronation. METHODS: Six fresh-frozen cadaveric upper extremities (mean age at the time of death, 79.4+/-2.8 years) were used to quantify loss of forearm rotation after simulation of axial rotational malunions of the ulna. First, maximum forearm rotation in supination and pronation was measured at torques of 6.8, 13.6, and 20.4 kilograms-centimeter applied with use of a custom jig. Following a midshaft ulnar osteotomy, a custom adjustable internal fixation plate was used to simulate axial rotational malunions of the ulna of 0, 15, 30, and 45 degrees in both directions. Measurements in supination and pronation were then repeated at the prespecified torques. Analysis of variance, with a p value of 0.05, was used for statistical analysis. RESULTS: In all instances, a decrease in forearm rotation after simulation of the ulnar rotational malunion was accompanied by an increase in rotation in the opposite direction. Supination and pronation were significantly influenced, whereas the total arc of rotation was not affected by ulnar rotational malunion. At a torque of 20.4 kilograms-centimeter, pronation malunions of 15, 30, and 45 degrees resulted in a mean loss of supination (and standard error of the mean) of 5+/-1, 11+/-1, and 20+/-1 degrees, respectively, and supination malunions of 15, 30, and 45 degrees resulted in a mean loss of pronation of 4+/-1, 10+/-2, and 18+/-4 degrees, respectively. The ratio of the simulated rotational malunion to the loss of motion was larger than one. CONCLUSIONS: Ulnar rotational malunions do not lead to a significant change in the total arc of forearm rotation. Instead, loss of motion in one direction is accompanied by increased motion in the opposite direction. Even with a 45-degree ulnar rotational malunion, forearm rotation decreases no more than 20 degrees.

Noninterferometric quantitative phase imaging with soft x rays.

We demonstrate quantitative noninterferometric x-ray phase-amplitude measurement. We present results from two experimental geometries. The first geometry uses x rays diverging from a point source to produce high-resolution holograms of submicrometer-sized objects. The measured phase of the projected image agrees with the geometrically determined phase to within +/-7%. The second geometry uses a direct imaging microscope setup that allows the formation of a magnified image with a zone-plate lens. Here a direct measure of the object phase is made and agrees with that of the magnified object to better than +/-10%. In both cases the accuracy of the phase is limited by the pixel resolution.

Electromyographic analysis of deltoid and rotator cuff function under varying loads and speeds.

The purpose of this study was to compare the effect of increasing loads and doubling speed on the deltoid and rotator cuff muscles during isotonic scapular plane abduction (scaption) with neutral humeral rotation. These muscles were studied in 16 volunteers with asymptomatic shoulders with the use of fine wire electromyography. The addition of load to the arm during scaption caused an increase in electromyographic activity during the first 90 degrees of motion. Furthermore electromyographic activity decreased during the final 30 degrees of motion with each increase in load. Doubling the speed caused an increase in electromyographic activity during the first 60 degrees of motion while causing a decrease in activity in the final 60 degrees. This study demonstrates the response of the rotator cuff and deltoid muscles to varying loads and speeds during the most basic shoulder motion. With the data obtained in this study, rehabilitation exercises and experimental shoulder models can be refined to reflect this more physiologic situation.

Glenohumeral translation after arthroscopic thermal capsuloplasty with a radiofrequency probe.

The purpose of this study was to determine whether there are changes in anterior and posterior glenohumeral translation after arthroscopic thermal capsuloplasty with a radiofrequency probe. Anteriorly directed loads of 15 N and 20 N were sequentially applied to the humerus of each of 5 cadaveric glenohumeral joints, and anterior translation on the glenoid was measured through use of a customized translation apparatus and an electromagnetic tracking device. The tests were then repeated with posteriorly directed forces, and posterior translation was measured. During testing, the glenoid was rigidly fixed and the glenohumeral joint was positioned to simulate 90 degrees of shoulder abduction and 90 degrees of external rotation. By means of the radiofrequency probe, thermal energy was then applied to the anteroinferior capsuloligamentous structures; anterior and posterior translation measurements were repeated. The results showed a significant reduction in anterior and posterior translations after thermal capsuloplasty (P < .05). Anterior translation decreased from 6.8 to 4.0 mm (a 41% decrease) with the 15-N load and from 8.6 to 4.9 mm (a 42% decrease) with the 20-N load. Posterior translation decreased from 9.3 to 5.8 mm (a 36% decrease) with the 15-N load and from 10.4 to 6.5 mm (a 35% decrease) with the 20-N load. The results of this study indicate that the radiofrequency probe can be used to decrease both anterior and posterior glenohumeral translation in vitro. The biological effect on heat-treated tissues over time needs to be studied to prove that this is a satisfactory treatment for glenohumeral instability.

The painful shoulder. Zeroing in on the most common causes.

Most shoulder problems seen by the primary care physician involve impingement syndrome or problems at the acromioclavicular joint. Despite the complexity of the structures involved, most of these conditions can be diagnosed and treated without difficulty. MRI or other imaging studies are seldom needed but can be used to confirm a questionable diagnosis. Referral to an orthopedic surgeon is appropriate if shoulder problems persist for 3 to 6 months or if there is evidence of a medium or large rotator cuff tear, severe shoulder stiffness, or a complicated fracture.