Subject(s)
Pharmacy Service, Hospital/organization & administration , Humans , Medication Systems, Hospital , Mississippi , Outcome and Process Assessment, Health Care , Personnel Staffing and Scheduling , Pharmacists , Pharmacy Service, Hospital/trends , Pilot Projects , Task Performance and Analysis , WorkforceABSTRACT
Three cases are presented which illustrate the possible difficulties in differentiating between the diagnoses of Guillain-Barre Syndrome and conversion disorder. Accepted criteria are specified for each condition, as well as some associated features often characteristic of similar cases. Supportive interdisciplinary treatment for Guillain-Barre Syndrome is reviewed, and an interdisciplinary multi-modal approach to treatment of conversion disorder is described.
Subject(s)
Conversion Disorder/diagnosis , Polyradiculoneuropathy/diagnosis , Adolescent , Child , Conversion Disorder/psychology , Conversion Disorder/therapy , Diagnosis, Differential , Female , Humans , Male , Polyradiculoneuropathy/therapyABSTRACT
Survey data were collected from a sample of 298 occupational therapy department directors on (a) department demographics; (b) availability of micro- or macrocomputers; (c) types of hardware, software, and peripheral devices used; (d) major purposes and functions for computers; and (e) major factors regarding choice of computers and equipment or factors most influential in the nonuse of computers. Usable questionnaires were returned from 238 or 80% of the sample. Thirty-five percent of the respondents had access to microcomputers in their work setting; however, only 11% actually had them in their occupational therapy department. The study also noted the versatility and adaptability of the computer as used in occupational therapy and discussed common limitations in its use.
Subject(s)
Computers , Occupational Therapy/instrumentation , Microcomputers , Surveys and Questionnaires , United StatesABSTRACT
Analysis of human glyceraldehyde-3-phosphate dehydrogenase mRNA revealed that levels in adult skeletal muscle are 12-fold greater per microgram of polyadenylated RNA than in fetal skeletal muscle, whereas in cardiac muscle RNA levels were about equal in fetal and adult tissue. The mRNA levels correlate well with glyceraldehyde 3-phosphate dehydrogenase enzyme activities. There was no evidence for fetus- or tissue-specific forms.
Subject(s)
Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , Muscles/enzymology , RNA, Messenger/genetics , Adult , Cell Line , Embryonic and Fetal Development , Female , Heart/embryology , Humans , Isoenzymes/genetics , Myocardium/enzymology , Nucleic Acid Hybridization , PregnancyABSTRACT
Our previous studies using rodent/human somatic-cell hybrids suggested that the expression of human mitochondrial glycerol-3-phosphate dehydrogenase (GPDM) is dependent on the presence of human mitochondria. This has now been tested directly by analysis of GPDM activity in a series of nine hybrid-cell lines, four segregating human chromosomes and five losing rodent chromosomes (reverse segregants). The chromosome composition of the hybrids was deduced from analysis of biochemical markers and examination of G- and G11-banded metaphase spreads and the mitochondrial content was determined by Southern blot analysis, using cloned mouse and human mtDNA sequences as probes. We found that the mtDNA species present in these hybrids correlated exactly with the pattern of chromosome segregation such that the conventional hybrids contained rodent mtDNA and the reverse segregants human mtDNA. However, the pattern of GPDM expression was not directly correlated with the species of chromosomes or mitochondria present: all the hybrids showed strong rodent GPDM activity and two from each class of hybrid also showed human GPDM activity but the other hybrids were negative for human GPDM. We conclude that rodent GPDM readily integrates into human mitochondria, that the expression of rodent GPDM is not dependent on the presence of rodent mitochondria, and that GPDM is not coded by mtDNA. Human GPDM either is not capable of being inserted into the rodent mitochondrial membrane or is regulated in some way in the hybrid cells by an unidentified rodent factor.
Subject(s)
Glycerolphosphate Dehydrogenase/genetics , Hybrid Cells/enzymology , Animals , Cell Line , Cricetinae , DNA Restriction Enzymes , DNA, Mitochondrial/genetics , Humans , Isoenzymes/genetics , Karyotyping , Liver Neoplasms, Experimental/enzymology , Mice , Mitochondria/enzymology , Neuroblastoma/enzymology , Rats , Species SpecificityABSTRACT
The parental origin of mitochondria in hydatidiform moles has been investigated by analysis of genetic variants of mtDNA restriction enzyme patterns. In six complete moles the mtDNA was found to be maternal in origin, with no contribution from the sperm mitochondria, while the nuclear genome was shown to be exclusively paternal in five cases. The occurrence of mtDNA variation in the healthy population was investigated using white blood cells and placentae, and the most common variation occurred at the Ava II restriction sites. The variants exhibited by molar mtDNA were the same as those found in material from healthy individuals.
Subject(s)
DNA, Mitochondrial/genetics , Hydatidiform Mole/genetics , Uterine Neoplasms/genetics , DNA Restriction Enzymes , DNA, Neoplasm/genetics , Female , Genetic Variation , Heterozygote , Homozygote , Humans , Hydatidiform Mole/analysis , Leukocytes/analysis , Mitochondria/analysis , Pedigree , Placenta/analysis , Pregnancy , Uterine Neoplasms/analysisABSTRACT
Pediatricians are often asked to assist families planning or undergoing divorce. Parents generally seek information regarding how and what to tell their children; others bring concerns about their children's emotional responses and subsequent behavior. This article reviews the more common responses of children and parents to the divorce process. Developmental concerns are highlighted, and possible responses to parental questions regarding problems of visitation, dating, and discipline are reviewed. The magnitude of the distress experienced by children of divorce may be related to the failure of parents to understand their children's responses and to provide comforting reassurances. The role of the pediatrician in relieving this distress by counseling parents is emphasized.
