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2.
Ann Neurol ; 83(1): 142-152, 2018 01.
Article in English | MEDLINE | ID: mdl-29283444

ABSTRACT

OBJECTIVE: Microvascular brain injury (mVBI) is a common pathological correlate of vascular contributions to cognitive impairment and dementia (VCID) that leads to white matter (WM) injury (WMI). VCID appears to arise from chronic recurrent white matter ischemia that triggers oxidative stress and an increase in total oligodendrocyte lineage cells. We hypothesized that mVBI involves vasodilator dysfunction of white matter penetrating arterioles and aberrant oligodendrocyte progenitor cell (OPC) responses to WMI. METHODS: We analyzed cases of mVBI with low Alzheimer's disease neuropathological change in prefrontal cortex WM from rapid autopsies in a population-based cohort where VCID frequently occurs. Arteriolar vasodilator function was quantified by videomicroscopy. OPC maturation was quantified using lineage specific markers. RESULTS: Acetylcholine-mediated arteriolar dilation in mVBI was significantly reduced in WM penetrators relative to pial arterioles. Astrogliosis-defined WMI was positively associated with increased OPCs and was negatively associated with decreased mature oligodendrocytes. INTERPRETATION: Selectively impaired vasodilator function of WM penetrating arterioles in mVBI occurs in association with aberrant differentiation of OPCs in WMI, which supports that myelination disturbances in VCID are related to disrupted maturation of myelinating oligodendrocytes. Ann Neurol 2018;83:142-152.


Subject(s)
Aging/pathology , Oligodendroglia/pathology , Vasodilation , White Matter/pathology , Acetylcholine/pharmacology , Aged , Aged, 80 and over , Alzheimer Disease/pathology , Arterioles/pathology , Autopsy , Cell Lineage , Cognition Disorders/pathology , Cohort Studies , Female , Gliosis/pathology , Humans , Immunohistochemistry , Male , Neural Stem Cells/pathology , Prefrontal Cortex/pathology
3.
J Neuropathol Exp Neurol ; 75(6): 539-54, 2016 06.
Article in English | MEDLINE | ID: mdl-27142644

ABSTRACT

Cerebral white matter injury (WMI) contributes to cognitive dysfunction associated with pathological aging. Because reactive astrocyte-related factors contribute to remyelination failure after WMI, we sought accurate, cost-effective, and reproducible histopathological approaches for quantification of morphometric features of reactive astrogliosis in aged human white matter in patients with vascular brain injury (VBI). We compared 7 distinct approaches to quantify the features of glial fibrillary acidic protein (GFAP)-labeled astrocytes in the prefrontal white matter of brains from patients with VBI (n = 17, mean age 88.8 years) and controls that did not exhibit VBI (n = 11, mean age 86.6 years). Only modern stereological techniques (ie, optical fractionator and spaceballs) and virtual process thickness measurements demonstrated significant changes in astrocyte number, process length, or proximal process thickness in cases with VBI relative to controls. The widely employed methods of neuropathological scoring, antibody capture assay (histelide), area fraction fractionator, and Cavalieri point counting failed to detect significant differences in GFAP expression between the groups. Unbiased stereological approaches and virtual thickness measurements provided the only sensitive and accurate means to quantify astrocyte reactivity as a surrogate marker of WMI in human brains with VBI.


Subject(s)
Aging/pathology , Cerebrovascular Trauma/pathology , Glial Fibrillary Acidic Protein/biosynthesis , Gliosis/pathology , Leukoencephalopathies/pathology , Stereotaxic Techniques , Aged , Aged, 80 and over , Aging/metabolism , Animals , Cerebrovascular Trauma/metabolism , Cross-Sectional Studies , Female , Gliosis/metabolism , Humans , Leukoencephalopathies/metabolism , Male , Population Surveillance , White Matter/metabolism , White Matter/pathology
4.
Exp Brain Res ; 232(12): 3929-38, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25163672

ABSTRACT

We investigated recovery of precision grasping of small objects between the index finger and thumb of the impaired hand without forced use after surgically placed lesions to the hand/arm areas of M1 and M1 + lateral premotor cortex in two monkeys. The unilateral lesions were contralateral to the monkey's preferred hand, which was established in prelesion testing as the hand used most often to acquire raisins in a foraging board (FB) task in which the monkey was free to use either hand to acquire treats. The lesions initially produced a clear paresis of the contralesional hand and use of only the ipsilesional hand to acquire raisins in the FB task. However, beginning about 3 weeks after the lesion both monkeys spontaneously began using the impaired contralesional hand in the FB task and increased use of that hand over the next few tests. Moreover, the monkeys clearly used precision grasp to acquire the raisins in a similar manner to prelesion performances, although grasp durations were longer. Although the monkeys used the contralesional hand more often than the ipsilesional hand in some postlesion testing sessions, they did not recover to use the hand as often as in prelesion testing when the preferred hand was used almost exclusively. These findings suggest that recovery of fine hand/digit motor function after localized damage to the lateral frontal motor areas in rhesus monkeys does not require forced use of the impaired hand.


Subject(s)
Brain Injuries/physiopathology , Hand Strength/physiology , Motor Cortex/physiopathology , Motor Skills/physiology , Recovery of Function/physiology , Animals , Functional Laterality/physiology , Hand/physiopathology , Macaca mulatta , Male , Motor Cortex/injuries
5.
Hand (N Y) ; 9(1): 105-11, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24570646

ABSTRACT

PURPOSE: The A2 and A4 pulleys have been shown to be important in finger flexor tendon function. Other authors have suggested either reconstruction or venting of portions of these pulleys in an attempt to preserve finger function in certain clinical situations. This study examines the effects of partial incision of these pulleys on finger flexion kinematics and biomechanics. METHODS: The index and ring fingers of 16 cadaveric hands were studied. The flexor digitorum profundus tendon was isolated and attached to a computer driven servo-motor. Micro-potentiometers measured flexion angles of the metacarpophalangeal, proximal inter-phalangeal and distal inter-phalangeal joints. Joint inertial torques were calculated making use of this experimental kinematic data. RESULTS: Proximal 50 % incisions of either the A2 or the A4 pulleys resulted in a statistically significant decrease in overall finger motion. This effect was greatest in the proximal inter-phalangeal joint, with a decrease in joint motion, as well as an earlier time to initiation of motion. These changes in finger motion were more pronounced with A2 pulley incision than they were with A4 pulley incision, but the changes were statistically significant in either case. No significant changes in joint inertial torques were shown. CONCLUSIONS: Our data provides evidence to the importance of the proximal portions of the A2 and A4 pulleys, and may support partial distal incision of these pulleys in certain clinical situations.

6.
J Comp Neurol ; 521(18): 4205-35, 2013 Dec 15.
Article in English | MEDLINE | ID: mdl-23840034

ABSTRACT

To further our understanding of the corticospinal projection (CSP) from the hand/arm representation of the primary motor cortex (M1), high-resolution anterograde tracing methodology and stereology were used to investigate the terminal distribution of this connection at spinal levels C5 to T1. The highest number of labeled terminal boutons occurred contralaterally (98%) with few ipsilaterally (2%). Contralaterally, labeled boutons were located within laminae I-X, with the densest distribution found in lamina VII and, to a lesser extent, laminae IX and VI. Fewer terminals were found in other contralateral laminae. Within lamina VII, terminal boutons were most prominent in the dorsomedial, dorsolateral, and ventrolateral subsectors. Within lamina IX, the heaviest terminal labeling was distributed dorsally. Ipsilaterally, boutons were found in laminae V-X. The most pronounced distribution occurred in the dorsomedial and ventromedial sectors of lamina VII and fewer labeled boutons were located in other ipsilateral laminae. Segmentally, contralateral lamina VII labeling was highest at levels C5-C7. In contrast, lamina IX labeling was highest at C7-T1 and more widely dispersed among the quadrants at C8-T1. Our findings suggest dominant contralateral influence of the M1 hand/arm CSP, a contralateral innervation pattern in lamina VII supporting Kuypers (1982) conceptual framework of a "lateral motor system," and a projection to lamina IX indicating significant influence on motoneurons innervating flexors acting on the shoulder and elbow rostrally (C5-C7), along with flexors, extensors, abductors and adductors acting on the digits, hand and wrist caudally (C8-T1).


Subject(s)
Arm/innervation , Hand/innervation , Macaca mulatta/anatomy & histology , Motor Cortex/cytology , Pyramidal Tracts/cytology , Animals , Female , Male
7.
Handb Clin Neurol ; 110: 13-21, 2013.
Article in English | MEDLINE | ID: mdl-23312627

ABSTRACT

Over the past two decades, results from neurophysiological studies in animal models and neuroimaging studies in human populations have converged along a common thread. Neuroplasticity in the remaining, intact tissue accompanies functional recovery after brain injury. Now, virtually every new therapeutic approach in postinjury rehabilitation relies on the fundamental principles of neuroplasticity for theoretical validity. In this chapter, the basic tenets of plasticity are outlined, and the neural substrates in the cerebral cortex that may subserve recovered functions are reviewed.


Subject(s)
Brain Diseases , Cerebral Cortex/physiology , Neuronal Plasticity/physiology , Recovery of Function , Animals , Brain Diseases/pathology , Brain Diseases/physiopathology , Cerebral Cortex/cytology , Humans
8.
Exp Neurol ; 231(1): 56-71, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21703261

ABSTRACT

Damage to the motor cortex of one hemisphere has classically been associated with contralateral upper limb paresis, but recent patient studies have identified deficits in both upper limbs. In non-human primates, we tested the hypothesis that the severity of ipsilesional upper limb motor impairment in the early post-injury phase depends on the volume of gray and white matter damage of the motor areas of the frontal lobe. We also postulated that substantial recovery would accompany minimal task practice and that ipsilesional limb recovery would be correlated with recovery of the contralesional limb. Gross (reaching) and fine hand motor functions were assessed for 3-12 months post-injury using two motor tests. Volumes of white and gray matter lesions were assessed using quantitative histology. Early changes in post-lesion motor performance were inversely correlated with white matter lesion volume indicating that larger lesions produced greater decreases in ipsilesional hand movement control. All monkeys showed improvements in ipsilesional hand motor skill during the post-lesion period, with reaching skill improvements being positively correlated with total lesion volume indicating that larger lesions were associated with greater ipsilesional motor skill recovery. We suggest that reduced trans-callosal inhibition from the lesioned hemisphere may play a role in the observed skill improvements. Our findings show that significant ipsilesional hand motor recovery is likely to accompany injury limited to frontal motor areas. In humans, more pronounced ipsilesional motor deficits that invariably develop after stroke may, in part, be a consequence of more extensive subcortical white and gray matter damage.


Subject(s)
Brain Injuries/pathology , Functional Laterality/physiology , Motor Cortex/injuries , Motor Cortex/pathology , Paresis/physiopathology , Recovery of Function/physiology , Animals , Brain Injuries/complications , Brain Injuries/physiopathology , Disease Models, Animal , Hand/innervation , Hand/physiopathology , Macaca mulatta , Motor Cortex/physiopathology , Nerve Fibers, Myelinated/pathology , Neural Pathways/injuries , Neural Pathways/pathology , Neural Pathways/physiopathology , Severity of Illness Index
9.
Exp Brain Res ; 202(3): 529-42, 2010 May.
Article in English | MEDLINE | ID: mdl-20107980

ABSTRACT

The purpose of this study was to determine if recovery of neurologically impaired hand function following isolated motor cortex injury would occur without constraint of the non-impaired limb, and without daily forced use of the impaired limb. Nine monkeys (Macaca mulatta) received neurosurgical lesions of various extents to arm representations of motor cortex in the hemisphere contralateral to the preferred hand. After the lesion, no physical constraints were placed on the ipsilesional arm/hand and motor testing was carried out weekly with a maximum of 40 attempts in two fine motor tasks that required use of the contralesional hand for successful food acquisition. These motor tests were the only "forced use" of the contralesional hand. We also tested regularly for spontaneous use of the contralesional hand in a fine motor task in which either hand could be used for successful performance. This minimal intervention was sufficient to induce recovery of the contralesional hand to such a functional level that eight of the monkeys chose to use that hand on some trials when either hand could be used. Percentage use of the contralesional hand (in the task when either hand could be used) varied considerably among monkeys and was not related to lesion volume or recovery of motor skill. These data demonstrate a remarkable capacity for recovery of spontaneous use of the impaired hand following localized frontal lobe lesions. Clinically, these observations underscore the importance of therapeutic intervention to inhibit the induction of the learned nonuse phenomenon after neurological injury.


Subject(s)
Arm/physiology , Brain Injuries/rehabilitation , Motor Cortex/physiology , Recovery of Function/physiology , Animals , Arm/innervation , Brain Injuries/physiopathology , Disease Models, Animal , Exercise Therapy/methods , Female , Functional Laterality/physiology , Macaca mulatta , Male , Motor Cortex/injuries , Paresis/etiology , Paresis/rehabilitation , Random Allocation
10.
J Comp Neurol ; 518(5): 586-621, 2010 Mar 01.
Article in English | MEDLINE | ID: mdl-20034062

ABSTRACT

Brain injury affecting the frontal motor cortex or its descending axons often causes contralateral upper extremity paresis. Although recovery is variable, the underlying mechanisms supporting favorable motor recovery remain unclear. Because the medial wall of the cerebral hemisphere is often spared following brain injury and recent functional neuroimaging studies in patients indicate a potential role for this brain region in the recovery process, we investigated the long-term effects of isolated lateral frontal motor cortical injury on the corticospinal projection (CSP) from intact, ipsilesional supplementary motor cortex (M2). After injury to the arm region of the primary motor (M1) and lateral premotor (LPMC) cortices, upper extremity recovery is accompanied by terminal axon plasticity in the contralateral CSP but not the ipsilateral CSP from M2. Furthermore, significant contralateral plasticity occurs only in lamina VII and dorsally within lamina IX. Thus, selective intraspinal sprouting transpires in regions containing interneurons, flexor-related motor neurons, and motor neurons supplying intrinsic hand muscles, which all play important roles in mediating reaching and digit movements. After recovery, subsequent injury of M2 leads to reemergence of hand motor deficits. Considering the importance of the CSP in humans and the common occurrence of lateral frontal cortex injury, these findings suggest that spared supplementary motor cortex may serve as an important therapeutic target that should be considered when designing acute and long-term postinjury patient intervention strategies aimed to enhance the motor recovery process following lateral cortical trauma.


Subject(s)
Brain Injuries/physiopathology , Frontal Lobe/physiology , Motor Cortex/physiology , Neuronal Plasticity/physiology , Pyramidal Tracts/physiology , Animals , Arm/innervation , Arm/physiopathology , Axons/physiology , Axons/ultrastructure , Brain Mapping , Dextrans , Disease Models, Animal , Female , Fluorescein , Frontal Lobe/anatomy & histology , Functional Laterality/physiology , Interneurons/cytology , Interneurons/physiology , Macaca mulatta , Male , Motor Cortex/anatomy & histology , Motor Cortex/injuries , Motor Neurons/cytology , Motor Neurons/physiology , Muscle, Skeletal/innervation , Muscle, Skeletal/physiopathology , Nerve Regeneration/physiology , Neuroanatomical Tract-Tracing Techniques , Paresis/physiopathology , Pyramidal Tracts/anatomy & histology , Recovery of Function/physiology , Spinal Cord/cytology , Spinal Cord/physiology , Time , Time Factors
11.
Exp Neurol ; 220(1): 90-108, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19679127

ABSTRACT

Due to the heterogeneous nature of most brain injuries, the contributions of gray and white matter involvement to motor deficits and recovery potential remain obscure. We tested the hypothesis that duration of hand motor impairment and recovery of skilled arm and hand motor function depends on the volume of gray and white matter damage of the frontal lobe. Lesions of the primary motor cortex (M1), M1 + lateral premotor cortex (LPMC), M1 + LPMC + supplementary motor cortex (M2) or multifocal lesions affecting motor areas and medial prefrontal cortex were evaluated in rhesus monkeys. Fine hand motor function was quantitatively assessed pre-lesion and for 3-12 months post-lesion using two motor tests. White and gray matter lesion volumes were determined using histological and quantitative methods. Regression analyses showed that duration of fine hand motor impairment was strongly correlated (R(2)>0.8) with the volume of gray and white matter lesions, with white matter lesion volume being the primary predictor of impairment duration. Level of recovery of fine hand motor skill was also well correlated (R(2)>0.5) with gray and white matter lesion volume. In some monkeys post-lesion skill exceeded pre-lesion skill in one or both motor tasks demonstrating that continued post-injury task practice can improve motor performance after localized loss of frontal motor cortex. These findings will assist in interpreting acute motor deficits, predicting the time course and expected level of functional recovery, and designing therapeutic strategies in patients with localized frontal lobe injury or neurosurgical resection.


Subject(s)
Brain Injuries/pathology , Motor Cortex/injuries , Motor Cortex/pathology , Movement Disorders/pathology , Recovery of Function/physiology , Animals , Biomechanical Phenomena , Brain Injuries/physiopathology , Disability Evaluation , Disease Models, Animal , Hand/innervation , Hand/physiopathology , Macaca mulatta , Motor Cortex/physiopathology , Motor Skills/physiology , Movement Disorders/physiopathology , Nerve Fibers, Myelinated/pathology , Neural Pathways/injuries , Neural Pathways/pathology , Neural Pathways/physiopathology , Paresis/etiology , Paresis/pathology , Paresis/physiopathology , Severity of Illness Index
12.
J Neurotrauma ; 24(11): 1719-42, 2007 Nov.
Article in English | MEDLINE | ID: mdl-18001202

ABSTRACT

Impaired fine motor functions after traumatic brain injury (TBI) in humans and non-human primates often continue to improve months after injury. To initiate a series of studies in the primate model designed to investigate possible involvement of microglia/macrophage in the long-term recovery processes, changes in these cells were studied in the rhesus monkey central nervous system at 1, 6, and 12 months after a combined unilateral lesion of the arm area of the primary motor cortex and arm area of the lateral premotor cortex. Immunohistological studies showed profound CD68 immunoreactivity in the lesion area and the contralateral lateral corticospinal tract in the spinal cord at all time points, demonstrating that microglia/macrophage remain reactive at the sites of injury and axonal degeneration/survival for at least 12 months. We also observed marked increases in brain-derived neurotrophic factor (BDNF) and its receptor subtypes, TrkB[gp145] and TrkB[TK-], around the cortical lesion site after 6-month survival. Similar increases were also observed in the spinal cord, although it was less apparent for TrkB[gp145]. Double-labeling revealed that a subpopulation of CD68-immunoreacitve microglia/macrophage co-expressed BDNF in the cortex and spinal cord, and also TrkB[gp145] or TrkB[TK-] in the spinal cord. In contrast, cytokine expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) at these time intervals was less prominent, suggesting that immediate inflammatory responses had subsided. These results demonstrate that microglia/macrophage undergo prolonged activation after TBI in the non-human primate brain and express BDNF and its receptors, suggesting their tropic/trophic roles in the long-term recovery processes.


Subject(s)
Brain Injuries/pathology , Gliosis/etiology , Spinal Cord/pathology , Animals , Antigens, CD/metabolism , Antigens, Differentiation, Myelomonocytic/metabolism , Brain Injuries/complications , Brain Injuries/metabolism , Brain-Derived Neurotrophic Factor/metabolism , Cervical Vertebrae , Gliosis/metabolism , Gliosis/pathology , Macaca mulatta , Macrophages/pathology , Male , Microglia/pathology , Receptor, trkB/metabolism , Spinal Cord/metabolism , Time Factors
13.
J Comp Neurol ; 504(2): 149-67, 2007 Sep 10.
Article in English | MEDLINE | ID: mdl-17626268

ABSTRACT

Motor deficit severity and the potential for recovery in patients with brain injury depend on the integrity of descending corticofugal projections. Clinical assessment of these conditions following subtotal brain trauma requires a comprehensive understanding of the anatomical structures involved in the lesion as well as those structures that are spared. To assist in this endeavor, we investigated motor fiber organization in the crus cerebri of the cerebral peduncle (ccCP) in the rhesus monkey. Fibers originating from the arm representations of the primary (M1), supplementary (M2), rostral cingulate (M3), caudal cingulate (M4), dorsolateral pre- (LPMCd) and ventrolateral pre- (LPMCv) motor cortices were studied. The projections from the frontal and cingulate motor cortices formed descending longitudinal bundles that occupied the medial three-fifths of the ccCP at superior and middle levels. Although considerable overlap characterized these corticofugal projections, a general topography was discernable. Fibers from M1 and M4 occupied the central subsector of the ccCP, and fibers from M3 resided medially. The main distribution of LPMCd, LPMCv, and M2 fibers occupied the centromedial region and overlapped extensively. Progressing inferiorly, all fiber bundles in the central and centromedial sectors gradually extended medially, and overlap increased. A common location of fiber passage occurred at the midbrain-pontine isthmus where all of the fiber bundles overlapped. Our findings indicate that the widespread distribution of corticofugal motor projections may account for the favorable levels of motor recovery that accompany subtotal midbrain injury. At superior and mid-levels of the ccCP anteromedial lesions may disrupt projections from M3, whereas anterolateral lesions may disrupt projections from M1 and M4. Fibers from M2, LPMCv, and LPMCd may be compromised to some degree in both situations. The compact and commixed nature of motor fiber organization at inferior levels and the midbrain-pontine isthmus suggests a vulnerable region of passage for comprehensive disruption of frontal and cingulate corticofugal projection fibers.


Subject(s)
Arm/innervation , Brain Mapping , Frontal Lobe/anatomy & histology , Gyrus Cinguli/anatomy & histology , Macaca mulatta/physiology , Mesencephalon/anatomy & histology , Animals , Arm/physiology , Electric Stimulation , Frontal Lobe/physiology , Gyrus Cinguli/physiology , Macaca mulatta/anatomy & histology , Mesencephalon/physiology , Neural Pathways/anatomy & histology , Neural Pathways/physiology
14.
J Neurophysiol ; 98(2): 1015-29, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17553948

ABSTRACT

A modified "Klüver" or dexterity board was developed to assess fine control of hand and digit movements by nonhuman primates during the acquisition of small food pellets from wells of different diameter. The primary advantages of the new device over those used previously include standardized positioning of target food pellets and controlled testing of each hand without the need for restraints, thereby allowing the monkey to move freely about the cage. Three-dimensional video analysis of hand motion was used to provide measures of reaching accuracy and grip aperture, as well as temporal measures of reach duration and food-pellet manipulation. We also present a validated performance score based on these measures, which serves as an indicator of successful food-pellet retrieval. Tests in three monkeys show that the performance score is an effective measure with which to study fine motor control associated with learning and handedness. We also show that the device and performance scores are effective for differentiating the effects of localized injury to motor areas of the cerebral cortex.


Subject(s)
Functional Laterality , Hand Strength/physiology , Hand/physiology , Movement/physiology , Numerical Analysis, Computer-Assisted/instrumentation , Psychomotor Performance/physiology , Animals , Behavior, Animal , Biomechanical Phenomena , Brain Mapping , Feeding Behavior/physiology , Macaca mulatta/physiology , Reproducibility of Results , Videotape Recording/methods
15.
J Comp Neurol ; 500(1): 134-65, 2007 Jan 01.
Article in English | MEDLINE | ID: mdl-17099887

ABSTRACT

Amygdala interconnections with the cingulate motor cortices were investigated in the rhesus monkey. Using multiple tracing approaches, we found a robust projection from the lateral basal nucleus of the amygdala to Layers II, IIIa, and V of the rostral cingulate motor cortex (M3). A smaller source of amygdala input arose from the accessory basal, cortical, and lateral nuclei, which targeted only the rostral region of M3. We also found a light projection from the lateral basal nucleus to the same layers of the caudal cingulate motor cortex (M4). Experiments examining this projection to cingulate somatotopy using combined neural tracing strategies and stereology to estimate the total number of terminal-like immunoreactive particles demonstrated that the amygdala projection terminates heavily in the face representation of M3 and moderately in its arm representation. Fewer terminal profiles were found in the leg representation of M3 and the face, arm, and leg representations of M4. Anterograde tracers placed directly into M3 and M4 revealed the amygdala connection to be reciprocal and documented corticofugal projections to the facial nucleus, surrounding pontine reticular formation, and spinal cord. Clinically, such pathways would be in a position to contribute to mediating movements in the face, neck, and upper extremity accompanying medial temporal lobe seizures that have historically characterized this syndrome. Alterations within or disruption of the amygdalo-cingulate projection to the rostral part of M3 may also have an adverse effect on facial expression in patients presenting with neurological or neuropsychiatric abnormalities of medial temporal lobe involvement. Finally, the prominent amygdala projection to the face region of M3 may significantly influence the outcome of higher-order facial expressions associated with social communication and emotional constructs such as fear, anger, happiness, and sadness.


Subject(s)
Amygdala/anatomy & histology , Amygdala/physiology , Gyrus Cinguli , Motor Cortex/anatomy & histology , Motor Cortex/physiology , Neural Pathways/anatomy & histology , Amino Acids/metabolism , Animals , Biotin/analogs & derivatives , Biotin/metabolism , Brain Mapping , Dextrans/metabolism , Electric Stimulation/methods , Fluorescent Dyes/metabolism , Macaca mulatta/anatomy & histology , Models, Anatomic , Neural Pathways/physiology , Tritium/metabolism
16.
J Neurosci Methods ; 154(1-2): 38-44, 2006 Jun 30.
Article in English | MEDLINE | ID: mdl-16464505

ABSTRACT

We present a modification of the automated movement assessment panel [Gash DM, Zhang Z, Umberger G, Mahood K, Smith M, Smith C, et al. An automated movement assessment panel for upper limb motor functions in rhesus monkeys and humans. J Neurosci Methods 1999;89:111-7] that incorporates a three-dimensional load cell to record forces applied by monkeys while manipulating food targets. The absolute force-time integral (total absolute impulse) is used to characterize the total of the applied forces over time as the food (carrot chip with a hole punched through the center) is manipulated and lifted from a flat surface (easiest task) and threaded over a straight rod (medium difficulty) or curved rod (highest difficulty). The total impulse can be measured even on unsuccessful attempts to acquire the food. Thus, it can be used to evaluate changes in performance even before successful acquisition occurs as in learning or recovery following a nervous system insult. We show from tests in three rhesus monkeys that the total absolute impulse measure is sensitive to task complexity, learning and lesion of frontal lobe motor areas (in one case) and that there is good reliability in day-to-day performance (even with long periods between performances) after the monkey has learned the task. Importantly, the task requires minimal training as the monkeys can be successful on even the most difficult of these tasks with one or two training sessions, yet performance improvements continue to occur over several testing sessions. Furthermore, the three levels of task difficulty permit analysis of a progression of ability.


Subject(s)
Psychomotor Performance/physiology , Animals , Conditioning, Operant/physiology , Data Collection , Functional Laterality , Hand/physiology , Hemiplegia/physiopathology , Macaca mulatta , Motor Cortex/physiology
17.
J Hand Surg Am ; 31(2): 228-35, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16473683

ABSTRACT

PURPOSE: The purpose of this study was to quantify the changes in the arc of digital flexion before and after metacarpophalangeal (MCP) silicone arthroplasty with a 30 degrees preflexed design. METHODS: Index, middle, and ring fingers of 4 fresh-frozen cadaver hands were used. Each hand was attached (palmar side up) to a custom test apparatus. The tendon was drawn by a small winch-type servomotor. Micropotentiometers that were attached to the centers of rotation of the MCP, distal interphalangeal, and proximal interphalangeal joints measured angular displacement before and after MCP arthroplasty as a function of tendon excursion. The data were analyzed comparing the angle of flexion initiation and the angular displacement as a function of tendon excursion before and after joint arthroplasty. RESULTS: There were no statistical differences in the angles of the MCP joints at rest, the order of initiation of joint flexion, and the overall degree of flexion between the unoperated fingers and the fingers that had surgery. There was, however, a trend toward delay in flexion initiation, an increase in the MCP angle at rest, and a decrease in torque after implant arthroplasty. CONCLUSIONS: The decrease in initiation of flexion of the MCP joint, although not statistically significant, probably was related to the 30 degrees of preflexion built into the implant. We also noted a trend of decreased flexion at the MCP joint and increased flexion at the proximal interphalangeal and distal interphalangeal joints. This trend may be advantageous in the reconstruction of hands that initially have an MCP joint flexion deformity. TYPE OF STUDY/LEVEL OF EVIDENCE: to come.


Subject(s)
Arthroplasty, Replacement, Finger , Metacarpophalangeal Joint/surgery , Biomechanical Phenomena , Cadaver , Finger Joint/physiology , Humans , Metacarpophalangeal Joint/physiology , Range of Motion, Articular/physiology , Software
18.
Virus Res ; 106(1): 35-42, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15522445

ABSTRACT

Age-dependent poliomyelitis (ADPM) or murine amyotrophic lateral sclerosis (ALS) is a murine paralytic disease triggered in immunosuppressed genetically-susceptible mice by infection with the arterivirus lactate dehydrogenase-elevating virus (LDV). This disease provides an animal model for ALS, affecting anterior horn neurons and resulting in neuroparalysis 2-3 weeks after LDV infection. We have tested the hypothesis that spinal cord apoptosis is a feature of the LDV-induced murine ALS, since apoptosis is postulated to be a causal factor in human ALS. Gene microarray analyses of spinal cords from paralyzed animals revealed upregulation of several genes associated with apoptosis. Spinal cord apoptosis was investigated further by TUNEL and activated caspase-3 assays, and was observed to emerge concurrent with paralytic symptoms in both neuronal and non-neuronal cells. Caspase-3-dependent apoptosis was also triggered in cultured macrophages by neurovirulent LDV infection. Thus, virus-induced spinal cord apoptosis is a pre-mortem feature of ADPM, which affects both neuronal and support cells, and may contribute to the pathogenesis of this ALS-like disease.


Subject(s)
Amyotrophic Lateral Sclerosis/pathology , Apoptosis , Arterivirus Infections/pathology , Lactate dehydrogenase-elevating virus/physiology , Macrophages/virology , Amyotrophic Lateral Sclerosis/physiopathology , Animals , Arterivirus Infections/physiopathology , Arterivirus Infections/virology , Cell Culture Techniques , Disease Models, Animal , Lactate dehydrogenase-elevating virus/pathogenicity , Mice , Mice, Inbred Strains , Spinal Cord/pathology
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