Individual- and community-level factors associated with the presence of adequate iodized salt in households in Bangladesh: a multilevel modelling approach.

BACKGROUND: The aim of this study is to estimate the factors at both the individual and community levels related to the adequacy of iodized salt in households in Bangladesh. METHODS: For this study we utilized the 2019 Multiple Indicator Cluster Survey data. A total of 61 242 households were chosen as samples from Bangladesh. In our study of socio-economic disparities, we applied a concentration indexing method. To identify the factors associated with the adequacy of iodine in salt at both the individual and community levels, we employed multilevel logistic regression. Aside from the multilevel regression used in the study, we also applied spatial analysis. RESULTS: The results indicated that the prevalence of iodine adequacy in household salt was found to be 57.8% (95% confidence interval 57.4 to 58.2). Rural areas have a higher concentration of iodine than urban areas. According to the multilevel model, younger women (adjusted odds ratio [aOR] 0.70), Muslim women (aOR 0.89), illiterate women (aOR 0.80) and those from poor households (aOR 0.33) were found to be less likely to consume iodine in concentrated salt compared with their counterparts. Disabled women and those with low media exposure have a lower likelihood of iodine adequacy in salt compared to their reference group. Furthermore, households in urban areas exhibited higher odds of having iodine adequacy in salt compared with households in rural areas. Barisal, Chattogram, Dhaka, Khulna, Mymensingh, Rajshahi and Rangpur Divisions have lower iodine adequacy in salt compared with Sylhet Division. CONCLUSIONS: The findings reveal that religion, physical disability and exposure to media exert an equal influence on the presence of iodized salt intake. Moreover, women's age, wealth status, education level and the educational background of the household head positively contribute to the adequacy of iodine in household salt. In light of these results, policymakers are advised to prioritize efforts aimed at enhancing iodine concentration, with a particular focus on mass media advertising, especially in rural areas (excluding Sylhet Division).

Determinants of household adoption of clean energy with its rural-urban disparities in Bangladesh.

This study aims to investigate factors influencing the adoption of clean energy among households in Bangladesh, using Blinder-Oaxaca decomposition and extended probit regression model with data from the 2019 Bangladesh multiple indicator cluster survey. Small households, primarily Muslim and urban dwellers, who speak the Bengali language and are Internet and mobile users, were likelier to adopt cleaner fuels than their counterparts. On the contrary, households residing in the Barisal, Khulna, Rajshahi, and Rangpur divisions, belonging to poor and middle-class households, with household heads aged 15-64 and without formal education, were less likely to adopt cleaner fuels than their counterparts. The concentration curve revealed socioeconomic inequality in the adoption of clean energy, particularly favouring richer households in urban and rural areas. Further analysis using the Blinder-Oaxaca decomposition showed that urban residents showed a higher probability of adopting clean energy, with a significant difference of 0.508 compared to rural areas. Regarding the endowment effect, poor wealth quintile contributed the most, followed by the ownership of rented dwellings and the middle wealth quintile. The Bengali differential effect made the largest contribution to this aspect of the disparity, followed by the exposure of the Internet and the influence of the Dhaka and Chattogram divisions. The detailed analysis provides valuable insights for policymakers and practitioners on the issue of disparities in the adoption of clean energy between urban and rural areas in Bangladesh.

Jute Stick-Derived Cellulose-Based Hydrogel: Synthesis, Characterization, and Methylene Blue Removal from Aqueous Solution.

In this work, microcrystalline cellulose (MCC) was isolated from jute sticks and sodium carboxymethyl cellulose (Na-CMC) was synthesized from the isolated MCC. Na-CMC is an anionic derivative of microcrystalline cellulose. The microcrystalline cellulose-based hydrogel (MCCH) and Na-CMC-based hydrogel (Na-CMCH) were prepared by using epichlorohydrin (ECH) as a crosslinker by a chemical crosslinking method. The isolated MCC, synthesized Na-CMC, and corresponding hydrogels were characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electronic microscopy (SEM), and energy dispersive spectroscopy (EDS) for functional groups, crystallinity, surface morphology, and composite elemental composition, respectively. Pseudo-first-order, pseudo-second-order, and Elovich models were used to investigate the adsorption kinetics. The pseudo-second-order one is favorable for both hydrogels. Freundlich, Langmuir, and Temkin adsorption isotherm models were investigated. MCCH follows the Freundlich model (R2 = 0.9967), and Na-CMCH follows the Langmuir isotherm model (R2 = 0.9974). The methylene blue (MB) dye adsorption capacities of ionic (Na-CMCH) and nonionic (MCCH) hydrogels in different contact times (up to 600 min), initial concentrations (10-50 ppm), and temperatures (298-318 K) were investigated and compared. The maximum adsorption capacity of MCCH and Na-CMCH was 23.73 and 196.46 mg/g, respectively, and the removal efficiency of MB was determined to be 26.93% for MCCH and 58.73% for Na-CMCH. The Na-CMCH efficiently removed the MB from aqueous solutions as well as spiked industrial wastewater. The Na-CMCH also remarkably efficiently reduced priority metal ions from an industrial effluent. An effort has been made to utilize inexpensive, readily available, and environmentally friendly waste materials (jute sticks) to synthesize valuable adsorbent materials.

Early Prediction and HRCT Evaluation of Post Covid-19 Related Lung Fibrosis.

Background: Early detection of post-COVID-19-related lung fibrosis is very important for the early introduction of treatment and to minimize morbidity and mortality. The aim of this study is the early detection and evaluation of post-COVID-19 fibrosis by high-resolution computed tomography (HRCT). Methods: This prospective study included 115 patients irrespective of age and sex, who tested positive for the SARS-CoV-2 by nasopharyngeal swab (RT PCR), admitted to the Dhaka North City Corporation (DNCC) dedicated COVID-19 hospital, Dhaka, and discharged after recovery. Patients went through a chest HRCT scan at least once during their hospital stay and another scan during follow-up after hospital discharge and 8 to 12 weeks of negative RT-PCR report. Result: Among 100 patients 23 patients had >50% of total lung involvement by visual assessment. Thirty-three patients had 25% to 50% of total lung volume involvement. Twenty-seven patients had less than 25% of total lung involvement, whereas 17 patients had no visual fibrotic change on the follow-up HRCT scan. A statistical association was found between age, gender, smoking, and severe form of lung fibrosis (P < .05). Patients with mild CT severity score (⩽8) had a very good prognosis. Patients who were admitted to the hospital for more than 15 days were more prone to developing moderate and severe forms of fibrosis. Patients who received at least 2 doses of the COVID-19 vaccine had less severe forms of fibrosis as well as more cases of complete radiological recovery. On the HRCT scan, most of the patients had bilateral, peripheral (68%), and predominant mid & lower lobar parenchymal involvement. Conclusion: Early detection and HRCT evaluation of post-COVID-19 related lung fibrosis is very crucial for early management and introduction of anti-fibrotic drugs.

Individual and community-level factors associated with discrimination among women aged 15-49 years in Bangladesh: Evidence based on multiple indicator cluster survey.

BACKGROUND: This research aimed to examine the factors at both the individual and community levels that are linked to discrimination experienced by women aged 15-49 in Bangladesh. METHODS: The relevant data was taken from the 2019 Multiple Indicator Cluster Survey in Bangladesh. The risk factors for discrimination against women in Bangladesh were determined using multilevel logistic regression models. RESULTS: The overall prevalence of discrimination against women was found to be 10.4% (95% CI: 10.1-10.6). Based on the final model (Model 1V), at the individual level higher odds of discrimination were observed among women from poor (AOR:1.21,95%CI: 1.12-1.32) and middle income households (AOR:1.12, 95%CI:1.02-1.22) compared to those from rich households etc. Women who have never used ICT were 1.27 times (AOR = 1.27, 95% CI = 1.07-1.51) higher odds of discrimination when compared with women who were ICT exposed. Respondents who married before 18 years 10% more likely to (AOR = 1.10, 95% CI:1.02-1.19) discriminated than women married aged 18 years old or above. Women from urban communities were 15% less likely to experience discrimination than their rural counterparts. In comparison to the Sylhet Division, women in the Barisal, Chattogram, Dhaka, Khulna Mymensingh, Rajshahi, and Rangpur Divisions were respectively 3.02, 1.84, 1.68, 2.06, 4.97, 4.06, and 1.74 times more likely to experience discrimination. CONCLUSION: Findings revealed that various individual-level factors such as wealth index, CEB, ICT exposure, marital status, functional difficulty, age, women's happiness, magazine and radio exposure, age at marriage, current contraceptive use, polygamy, husband beating, place of attack, and household head age were found to have a significant association with women discrimination. Community-level factors such as residence and division were also found to have a notable impact on discrimination. Policymakers should incorporate substantial components targeting both individual and community levels into intervention programs with the goal of raising awareness about women's discrimination.

Prevalence and Factors Associated with Alcohol Use among Dayak Adolescents in Sarawak, Malaysia

@#Introduction: Alcohol is classified as a psychoactive substance, and its dependence is one of the three leading risk factors for death, disability, or disease. Early age alcohol use by the age of 15 is implied to be harmful due to interference in the brain development of adolescents, causing alcohol-related problems later in life. This study aims to determine the prevalence and factors associated with alcohol use among Dayak adolescents in Sarawak, Malaysia. Methods: This was a cross-sectional quantitative research approach. A total of 12 districts within the 12 divisions were selected by multistage stratified cluster sampling. Dayak housing area lists were obtained from district offices and the local councils. The respondents were selected systematically according to male and female respondent houses, and they were interviewed using an interviewer-administered questionnaire. Data were entered into Microsoft Excel and analysed through IBM SPSS Version 27.0. Results: Dayak adolescents’ mean age was 17.31 years. They were either low-risk (50.0 %), hazardous (31.0%), abstainers (11.0%), harmful (5.0%) or alcohol dependant (3.0%) drinkers. Age (p<.001), gender (p<.05), ethnicity (p<.001), religion (p<.05), employment (p<.001), level of education (p<.01), allowance (p<.001), family relationship (p<.05), and family size (p<.05) were the potential predictors of alcohol use disorder. Conclusion: Dayak adolescent alcohol drinking is prevalent and a public health problem, particularly among disadvantaged socio-economic families. The lower proportion of abstainers suggested that alcohol use among adolescents is on the rise. Hence, the results should be utilised to do targeted health promotion regarding harmful alcohol use among adolescents since early use of alcohol among adolescents may lead to more severe health and non-health outcomes in the future.

Synthesis, characterization and application of a novel polyazo dye as a universal acid-base indicator.

A novel organic polyazo dye is synthesized by the diazotization of aromatic aniline, followed by coupling it with sulfanilic acid and N,N-dimethylaniline. Characterization was done by 1H-NMR, 13C-NMR, and FTIR spectroscopy. Differential scanning calorimetry (DSC) reveals that phase transition for this molecule is exothermic. The optical band gap is estimated from the absorption cutoff point using UV-Visible spectroscopy. Thermal gravimetric analysis (TGA) addresses the thermal stability of the molecule and is found to be at ∼250 °C. The structure of the synthesized molecule is analogous to that of methyl orange and contains three azo groups. These three azo groups help accept more than two protons and provide two pK a values when diprotic acid or a mixture of acids is used in different titrations. Specifically, when a polybasic acid is in strong base titration, the pK a values were found to be 3.5 and 9.1. Moreover, for strong base and (strong + weak) acid mixture titration, the pK a values are found to be 9.2 and 3.3. Furthermore, the pK a values are found to be 8.6 and 2.8 for (strong and weak) base mixture and (strong and weak) acid mixture titration, respectively. Owing to its increased proton accepting capacity, it can be found in the two pH ranges of 2.1-3.8 for orange color and 8.2-9.8 for yellow color, thus indicating a unique property as a universal indicator for acid-base titration. The dissociation constant of this dye is found to be 3.4 × 10-6, determined in a mixed aqueous solution of 10 wt% ethanol, and a linear relationship between pK a and pH is observed in this solvent system.

Hydrogeochemical processes and groundwater quality of over-exploited Dupi Tila aquifer in Dhaka city, Bangladesh.

Dhaka is one of the highly populated cities in the world. Increased urbanization and population growth in Dhaka are mostly dependent on groundwater, with 78% of the supply water coming from the Plio-Pleistocene Dupi Tila aquifer. This research was conducted with the objectives of identifying ion chemistry, hydrochemical processes and their relationship to groundwater quality and finding out the impact of over-exploitation on Dupi Tila aquifer. Three consecutive semi-confined aquifers have been delineated up to the explored depth: the Upper Dupi Tila aquifer (UDA), Middle Dupi Tila aquifer (MDA) and Lower Dupi Tila aquifer (LDA). Hydrogeochemical processes and water quality have been inferred from bivariate plots, correlation of major ions, piper plot and stable isotopes analysis. The total dissolved solids show an increasing downwards trend from an average of 267 mg/l in UDA to an average of 284 mg/l in LDA. Waters in all three aquifers as well as surface water (SW) are mainly of Ca/Mg-HCO3 type. The weathering of aluminosilicates controls the concentration of the major ions. Even though there is a significant decline in piezometric level both in UDA (> -85 m Public Works Department reference datum (PWD)) and MDA (> -65 m PWD), there is no evidence of groundwater recharge through direct infiltration from the river. The stable isotopes indicate most of the UDA, MDA and LDA waters are mainly from local precipitation. Increasingly enriched mean values for stable isotopes for the subsequent aquifers UDA, MDA and LDA, as well as increasing PCO2 for UDA < MDA < LDA, indicate increasingly warmer recharge conditions. The overall groundwater quality in the aquifers is good. At present, we have no indication that groundwater exploitation has altered groundwater quality of the Dupi Tila aquifer. Therefore, it is of vital importance to regularly monitor water quality for the purposes in order to timely detect any potential water quality alteration that could be aggravated by the vast decline in piezometric level, in view of the long-term sustainable development of the groundwater resources of this city.

A Single GUV Method for Revealing the Action of Cell-Penetrating Peptides in Biomembranes.

The mechanism of entry of cell-penetrating peptides (CPPs) into the cytosol of various cells has been studied by examining the interaction of CPPs with lipid bilayers and their entry into lipid vesicle lumens using various methods. Here we describe a single giant unilamellar vesicle (GUV) method to study CPPs. In this new method, we use GUVs containing small GUVs in the mother GUV lumen or GUVs containing large unilamellar vesicles (LUVs) in the GUV lumen and investigate the interaction of fluorescent probe-labeled CPPs with single GUVs in real time using confocal laser scanning microscopy. This method can detect CPPs in the GUV lumen with high sensitivity, allowing immediate measurement of the time course of entry of CPPs into the vesicle lumen. This method allows simultaneous measurement of the entry of CPPs and of CPP-induced pore formation, allowing the relationship between the two events to be determined. One can also simultaneously measure the entry of CPPs and the CPP concentration in the GUV membrane. The rate of entry of CPPs into a single GUV lumen can be estimated by obtaining the fraction of GUVs into which CPPs entered before a specific time t without pore formation among all examined GUVs (i.e., the fraction of entry) and the lumen intensity due to LUVs with bound CPPs. This method is therefore useful for elucidating the mechanism of entry of CPPs into lipid vesicles.

Gender-Stratified Factors Associated with Stigma Toward HIV/ AIDS among Rural Communities in Sarawak, Malaysia

@#Introduction: Many factors hinder the effective responses to the HIV/AIDS epidemic. Stigma is one of the major barriers to effective responses to the HIV/AIDS epidemic. It is often considered the main reason for the low response to access proper services for prevention, treatment, and care for HIV/AIDS. In this context, we assessed the perceived level of stigmatisation towards people with HIV/AIDS among rural communities in Sarawak and determined its associated factors. Methods: This cross-sectional community-based study was conducted among the adult population aged 18 years and above among rural communities in Sarawak, Malaysia. A gender-stratified multistage cluster sampling technique was adopted to select the respondents. A total of 900 respondents were successfully interviewed using interviewer-guided questionnaires. Stepwise multiple linear regression with forward and backward selection method was used to determine the HIV/AIDS-related stigma. A p-value of ≤.05 was considered statistically significant. Results: Analysis revealed that females are more stigmatised than males (p<.05). Multivariate analysis showed that those who did not know someone had HIV and Chinese ethnicity were potential predictors in both male and female groups. Having no gainful job, and discussion of HIV/AIDS, household income less than MYR 500, history of HIV testing, knowledge on HIV transmission and exposure to HIV/AIDS information were factors that influenced HIV/ AIDS-related stigma among female respondents. On the other hand, age group 30 to 39, Bidayuh, Chinese ethnicity, and not knowing someone had HIV and media exposure on HIV were factors that influenced HIV/AIDS-related stigma among male respondents. Conclusion: HIV/AIDS-related stigma towards HIV/AIDS existed in rural communities. Enhanced health promotion related to HIV/AIDS activities in rural communities are needed to curb stigmatisation toward people with HIV/AIDS.

A Comparative Study on the knowledge and attitude of COVID-19 among Urban and Rural populations of Bangladesh.

AimThis study is aimed to identify the awareness and behavioral perspective on COVID-19 between urban and rural people of Bangladesh during the period of outbreak. MethodsA community-based cross-sectional study was conducted in 30 districts of Bangladesh, where 322 participants from urban and 312 from rural area. Participants were selected through convenience sampling. ResultsRural people are found to be much more inter connected to receive information from neighbor. Regarding the incubation periods and the general symptoms, knowledge differs significantly from urban to rural. Even their precautionary and transmission knowledge is found to associate in most of the cases. During this outbreak, urban people significantly increase their religious habits and also believe that there will some major change of life after outbreak. DiscussionThe study reflected that health education program needed to aware about COVID-19 in both urban and rural in Bangladesh that helps in formulating and executing communication and outbreak management.

Pencil graphite as electrode platform for free chlorine sensors and energy storage devices.

Multifunctional and low-cost electrode materials are desirable for the next-generation sensors and energy storage applications. This paper reports the use of pencil graphite as an electrode for dual applications that include the detection of free residual chlorine using electro-oxidation process and as an electrochemical energy storage cathode. The pencil graphite is transferred to cellulose paper by drawing ten times and applied for the detection of free residual chlorine, which shows a sensitivity of 27 µA mM-1 cm-2 with a limit of detection of 88.9 µM and linearity up to 7 mM. The sample matrix effect study for the commonly interfering ions such as NO3-, SO42-, CO32-, Cl-, HCO3- shows minimal impact on free residual chlorine detection. Pencil graphite then used after cyclic voltammogram treatment as a cathode in the aqueous Zn/Al-ion battery, showing an average discharge potential plateau of ~1.1 V, with a specific cathode capacity of ~54.1 mAh g-1 at a current of 55 mA g-1. It maintains ~95.8% of its initial efficiency after 100 cycles. Results obtained from the density functional theory calculation is consistent with the electro-oxidation process involved in the detection of free residual chlorine, as well as intercalation and de-intercalation behavior of Al3+ into the graphite layers of Zn/Al-ion battery. Therefore, pencil graphite due to its excellent electro-oxidation and conducting properties, can be successfully implemented as low cost, disposable and green material for both sensor and energy-storage applications.

Groundwater salinization and freshening processes in coastal aquifers from southwest Bangladesh.

The hydrogeochemical processes in the multilayer aquifers of southwest (SW) coastal area of Bangladesh provide important information for quantifying hydrochemical differences between different aquifers. Hydrogeochemical processes affecting groundwater chemistry in this coastal area have been evaluated by interpreting conventional plots, ionic delta, HFE-diagram, stable isotopes and geochemical modelling. The median TDS distribution of the aquifers has an increasing trend from below 1000 mg/l in the deep aquifer (DA) to 2622 mg/l in lower shallow aquifer (LSA) and 7012 mg/l in upper shallow aquifer (USA). Na+ is the dominant cation in all the aquifers. HCO3- is the dominant anion in DA with high median concentration (495 mg/l), which is more than double than that in LSA (214 mg/l) and USA (159 mg/l). The groundwater in the DA is freshened NaHCO3+ type due to cation exchange process that enhances second stage of calcite dissolution. Few NaCl+ type DA waters found in the SW corner of the study area indicate the presence of connate water confined in the inter-basin during the Holocene transgression. The stable isotopes indicate that the DA waters have been deposited during warmer periods. In contrast, both the LSA and USA are dominated by NaCl- type water; some CaCl- type waters have also been found in these aquifers, indicating salinization. The salinization processes consist of infiltration of redissolved evaporite salts, reverse cation exchange, and mixing with marine influenced flood water. Thus, the knowledge of different processes controlling freshening and salinization will help properly manage and preserve the environmental characteristics in the aquifer systems of SW Bangladesh as well as similar complex coastal geological settings.

Polymorphic variants INSIG2 rs6726538, HLA-DRB1 rs9272143, and GCNT1P5 rs7780883 contribute to the susceptibility of cervical cancer in the Bangladeshi women.

OBJECTIVE: Cervical cancer is a gynecological health problem, affecting nearly 500,000 women each year worldwide. Genome-wide association studies have revealed multiple susceptible genes and their polymorphisms for cervical carcinoma risk. We have carried out this case-control study to investigate the association of INSIG2 rs6726538 (A; T), HLA-DRB1 rs9272143 (T; C), and GCNT1P5 rs7780883 (G; A) with cervical cancer. METHODS: The present study recruited 234 cervical cancer patients as cases and 212 healthy females as controls. We have applied the tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) method for genotyping. RESULTS: The SNP rs6726538 was significantly associated with increased risk of cervical cancer in all genetic models (AT vs. AA: OR = 3.30, 95% CI = 2.19-4.97, p < 0.0001; TT vs. AA: OR = 8.72, 95% CI = 3.87-19.7, p < 0.0001; AT+TT vs. AA: OR = 3.87, 95% CI = 2.61-5.73, p < 0.0001; T vs. A: OR = 2.97, 95% CI = 2.20-4.01, p < 0.0001) except the recessive model which showed a significantly reduced risk (TT vs. AA+AT: OR = 0.20, 95% CI = 0.09-0.44, p = 0.0001). rs9272143 showed significantly reduced risk for the additive model 1, dominant model, and allelic model (TC vs. TT: OR = 0.46, 95% CI = 0.31-0.70, p = 0.0004; TC+CC vs. TT: OR = 0.47 95% CI = 0.32-0.70, p = 0.0002; C vs. T: OR = 0.56, 95% CI = 0.40-0.78, p = 0.0006, respectively). The third variant, rs7780883, was significantly associated with increased risk in additive model 2, dominant, and allelic models (AA vs. GG: OR = 5.08, 95% CI = 2.45-10.5, p < 0.0001; GA+AA vs. GG: OR = 1.54, 95% CI = 1.06-2.24, p = 0.0237; A vs. G: OR = 1.88, 95% CI = 1.34-2.52, p < 0.0001, consecutively), whereas recessive model reduced the risk of cervical cancer (AA vs. GG+GA: OR = 0.20, 95% CI = 0.09-0.41, p < 0.0001). Other models of these SNPs were not associated with cervical cancer. All significant associations for three SNPs withstand after Bonferroni correction except the additive model 2 of rs7780883. CONCLUSION: Our study concludes that INSIG2 rs6726538, HLA-DRB1 rs9272143, and GCNT1P5 rs7780883 polymorphisms may contribute to the development of cervical cancer in the Bangladeshi population.

Large scale genomic and evolutionary study reveals SARS-CoV-2 virus isolates from Bangladesh strongly correlate with European origin and not with China.

RationaleThe global public health is in serious crisis due to emergence of SARS-CoV-2 virus. Studies are ongoing to reveal the genomic variants of the virus circulating in various parts of the world. However, data generated from low- and middle-income countries are scarce due to resource limitation. This study was focused to perform whole genome sequencing of 151 SARS-CoV-2 isolates from COVID-19 positive Bangladeshi patients. The goal of this study was to identify the genomic variants among the SARS-CoV-2 virus isolates in Bangladesh, to determine the molecular epidemiology and to develop a relationship between host clinical trait with the virus genomic variants. MethodSuspected patients were tested for COVID-19 using one step commercial qPCR kit for SARS-CoV-2 Virus. Viral RNA was extracted from positive patients, converted to cDNA which was amplified using Ion AmpliSeq SARS-CoV-2 Research Panel. Massive parallel sequencing was carried out using Ion AmpliSeq Library Kit Plus. Assembly of raw data is done by aligning the reads to a pre-defined reference genome (NC_045512.2) while retaining the unique variations of the input raw data by creating a consensus genome. A random forest-based association analysis was carried out to correlate the viral genomic variants with the clinical traits present in the host. ResultAmong the 151 viral isolates, we observed the 413 unique variants. Among these 8 variants occurred in more than 80 % of cases which include 241C to T, 1163A to T, 3037C to T,14408C to T, 23403A to G, 28881G to A, 28882 G to A, and finally the 28883G to C. Phylogenetic analysis revealed a predominance of variants belonging to GR clade, which have a strong geographical presence in Europe, indicating possible introduction of the SARS-CoV-2 virus into Bangladesh through a European channel. However, other possibilities like a route of entry from China cannot be ruled out as viral isolate belonging to L clade with a close relationship to Wuhan reference genome was also detected. We observed a total of 37 genomic variants to be strongly associated with clinical symptoms such as fever, sore throat, overall symptomatic status, etc. (Fishers Exact Test p-value<0.05). The most mention-worthy among those were the 3916CtoT (associated with causing sore throat, p-value 0.0005), the 14408C to T (associated with protection from developing cough, p-value= 0.027), and the 28881G to A, 28882G to A, and 28883G to C variant (associated with causing chest pain, p-value 0.025). ConclusionTo our knowledge, this study is the first large scale phylogenomic studies of SARS-CoV-2 virus circulating in Bangladesh. The observed epidemiological and genomic features may inform future research platform for disease management, vaccine development and epidemiological study.

Prevalence and factors associated with undernutrition among Dayak children in rural areas of Sarawak, Malaysia

@#Background: Undernutrition among children has become a major public health issue due to the high risk of morbidity and mortality involving this vulnerable age group. This study focused on the rural population, especially among the Dayak children in Sarawak, Malaysia. Methodology: This community-based cross-sectional study was conducted in Sarawak’s rural areas using a multistage stratified cluster sampling technique. Data were collected through face-to-face interviews using an interviewer-guided questionnaire. The nutritional status of the under-five children was measured according to the World Health Organization guidelines. The nutritional indicators were calculated as z-scores. Multivariate logistic regression analyses were performed using SPSS to identify the factors associated with nutritional status. Results: The prevalence of undernutrition among the under-five Dayak children in the rural areas of Sarawak was 39.6%. The analysis suggested that wasting was predominantly associated with parental education levels of primary and below, poor wealth index, environmental and sanitation issues, children with history of low birth weight, shorter duration of exclusive breastfeeding, and poor appetite. Stunting was high within the age group of <3 years old and was associated with parents’ occupation and household wealth index. Low parental education, poor wealth index, environmental and sanitation issues, poor appetite, and the children’s recent illness predicted underweight. Conclusion: These findings imply that a multi-sectoral and multi-dimensional approach is essential to address undernutrition in rural settings. Improvement on households’ socioeconomy, environment and sanitation should be emphasised to reduce undernutrition among the children.

Perceived Body Image And Its Relationship With Body Mass Index, Self-Esteem, Body Change Strategy, And Social Network Usage Among Rural Adolescents In Sarawak, Malaysia

@#Body image is a complex and multifaceted construct with multiple associated factors determining its perception among adolescents. This study aimed to determine the perceived body image and its relationship with body mass index, self-esteem, body change strategy, and social network usage among rural adolescents. This study was a cross-sectional study design using a multistage random sampling technique to select adolescents aged 10 to 19 years. A total of 318 adolescents’ data were collected using a self-administered questionnaire. Data analysis was done using SPSS version 22.0 with a p-value of <0.05 was considered statistically significant. The mean (SD) age of adolescents was 14.6 (2.7) years with a male and female ratio of 1.09:1. The mean score (SD) of perceived body image was 3.63 (0.87). Multiple linear regression analysis revealed that gender, body mass index, and strategy to increase weight could predict perceived body image among adolescents. However, self-esteem did not correlate with perceived body image. Although, the body image dissatisfaction level was low among rural adolescents compared to other studies in Malaysia. However, it can still pose a threat to adolescents’ health if not addressed accordingly.

Association Between Preventive Behaviour On Colorectal Cancer And Health Literacy Among The Adult Population In Sarawak, Malaysia

@#Health literacy has shown to have some influence in preventive behaviour, including on colorectal cancer. Such a relationship, however, is not well established in Malaysia, particularly Sarawak. This study is aimed to estimate the level of health literacy and preventive practice against colorectal cancer and factors associated with it. This was a cross-sectional study conducted in Sarawak, among adults aged 50 years and above. Data was collected by face to face interviews using a validated questionnaire. Descriptive statistics were used to determine the health literacy and preventive behaviour on colorectal cancer. Multiple linear regression was used to determine the factors associated with preventive behaviour on colorectal cancer and also to examine its relationship with health literacy. A p-value of ≤0.05 was considered as statistically significant. A total of 829 respondents were included in the analysis. Mean age (standard deviation) of the respondents was 55.78 (5.01) years. A large proportion had an educational background of upper secondary school and above (41%) and from Iban ethnicity (32.4%). Only 1.1% of them had a family history of colorectal cancer. Multiple linear regression analysis revealed that gender (p<0.01), ethnicity (p<0.05), functional health literacy (p<0.05), and knowledge on colorectal cancer (p<0.05) appeared to be significant predictors for preventive behaviour on colorectal cancer. Health literacy has a positive effect on preventive behaviour on colorectal cancer. The findings assist in designing and developing targeted informational campaigns to promote preventive behaviour, particularly among low health literate group.

Psychological Impact of Covid-19 Pandemic on Mental Health Among Medical Students in Malaysia

@#Introduction: The Coronavirus disease-2019 impacted the governments and public health systems worldwide, with the psychological impact including an increase in the level of stress, anxiety and depression. We determine the prevalence of depression, anxiety and stress using the Depression Anxiety Stress Scales (DASS-21), and to determine the psychological impact of COVID-19 pandemic amongst medical students in Sarawak, Malaysia using the revised Impact of Event Scale (IES-R). Methods: We analysed 355 medical student’s data obtained from an online survey. All the undergraduate medical students were the sample population. IBM SPSS version 22.0 was used for data analysis. A p-value of ≤.05 was considered statistically significant. Results: Analysis indicated that three-fourths of the students had no mental health issues. More than one-tenth of the students were moderately depressed (12.4%) and 4.3% severely depressed. A similar pattern of prevalence of anxiety was noted with 10% reported moderate anxiety, and 6.8% had severe anxiety. 15.8% of the students had mild stress, 4.2% moderately stressed, and 2% severely stressed. A hierarchical multiple linear regression analysis showed that hyperarousal appeared to be a predictor of depression (β=.466, p<.001), anxiety (β=.481, p<.001) and stress β=.508, p<.001). Along with hyperarousal, intrusion (β=.148, p<.05) predicts stress among the students. Conclusion: Our analysis revealed that hyperarousal symptoms related to the global pandemic of COVID-19 led to significant mental health issues among students. Therefore, urgent strategies to support the students’ psychosocial wellbeing and self-care and to strengthen their coping skills and resilience need to be implemented.

Acidochromic Behavior of Dibenzylidene Cyclohexanone-Based Bischalcone: Experimental and Theoretical Study.

Synthesis and characterization of substituted 2,6-dibenzylidene cyclohexanone-based bischalcone derivatives and their optimized geometries were investigated by density functional theory. The synthesized compounds were identified through ultraviolet-visible, Fourier transform infrared, and 1H nuclear magnetic resonance spectroscopies and elemental analysis. Significant acidochromic behavior was observed for 2,6-bis(4-dimethylamino-benzylidene)-cyclohexanone 1e. This result is owing to the preferential protonation of the chromophoric N,N-dimethylamino group, that is, quaternary salt formation and deactivation of the resonance system. The result was consistent with computational studies where the protonation was favored by 211 kcal/mol in the gas phase. The compounds also showed solvatochromic behavior. The geometries of the synthesized compounds were optimized with B3LYP/6-311G+(d,p) and APFD/6-311+G(d,p) basis sets. The single point energy indicated that APFD/6-311+G(d,p) basis set gave the lowest energy of 445-655 kcal/mol for the studied bischalcone derivatives. Quantum chemical parameters were also calculated.