Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
J Appl Microbiol ; 103(6): 2316-24, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18045416

ABSTRACT

AIMS: The contribution of the glutamate decarboxylase (GAD) acid resistance system to survival and growth of Listeria monocytogenes LO28 in modified atmosphere-packaged foods was examined. METHODS AND RESULTS: The survival and growth of the wild-type LO28 and four GAD deletion mutants (DeltagadA, DeltagadB, DeltagadC, DeltagadAB) in packaged foods (minced beef, lettuce, dry coleslaw mix) during storage at 4, 8 and 15 degrees C were studied. Survival and growth patterns varied with strain, product type, gas atmosphere and storage temperature. In minced beef, the wild-type LO28 survived better (P < 0.05) than the GAD mutant strains at 8 and 15 degrees C. In both packaged vegetables at all storage temperatures, the wild-type strain survived better (P < 0.05) than the double mutant DeltagadAB. The requirement for the individual gad genes varied depending on the packaged food. In the case of lettuce, gadA played the most important role, while the gadB and gadC genes played the greatest role in packaged coleslaw (at 15 degrees C). CONCLUSIONS: This work demonstrates that elements of the GAD system play significant roles in survival of L. monocytogenes LO28 during storage in modified atmosphere-packaged foods. SIGNIFICANCE AND IMPACT OF THE STUDY: A better understanding of how L. monocytogenes behaves in modified atmosphere-packaged foods, and how it responds to elevated carbon dioxide atmospheres.


Subject(s)
Food Microbiology , Food Packaging/methods , Glutamate Decarboxylase/metabolism , Listeria monocytogenes/enzymology , Microbial Viability , Antiporters/genetics , Bacterial Proteins/genetics , Carbon Dioxide , Food Preservation/methods , Gene Deletion , Lactuca , Listeria monocytogenes/growth & development , Listeria monocytogenes/metabolism , Listeriosis/transmission , Meat , Pressure , Risk , Temperature , Vegetables
2.
J Appl Microbiol ; 103(5): 1681-90, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17953579

ABSTRACT

AIMS: The aims of this research were (1) to determine the occurrence of Salmonella in Irish restaurant kitchens and (2) to investigate the serovar, genotype, antibiotic resistance profile and survival/growth profile of the Salmonella under catering chilled storage and temperature abuse conditions. METHODS: Five sites/tools in each of 200 randomly selected restaurant kitchens were examined for the presence of presumptive Salmonella spp. by enrichment. Serotyping, antibiotic resistance studies and genotyping were performed using the Kauffmann-White, CLSI and PulseNet methods, respectively. Survival/growth was investigated in milk, meat and vegetable products. RESULTS: Presumptive isolates from 15 of the 200 restaurant kitchens were recovered and confirmed as Salmonella positive. Seven different serovars showing a variety of antibiotic resistance profiles were detected. PFGE profiles suggested that isolates from geographically adjacent restaurants were related. Salmonella survived in foods stored at typical catering refrigeration temperatures and increased by approximately 0.8 log(10) CFU ml(-1) per day in food products stored under conditions of thermal abuse (20 degrees C). CONCLUSIONS: Inadequate hygiene has resulted in contamination of restaurant kitchens with Salmonella, which may persist/multiply in cross-contaminated foods. SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights the need for greater hygiene in restaurant kitchens coupled with rapid chilling of food not for immediate consumption and reheating before subsequent serving.


Subject(s)
Food Handling/standards , Food Microbiology , Restaurants , Salmonella Food Poisoning/prevention & control , Salmonella/isolation & purification , Animals , Bacteriological Techniques , Colony Count, Microbial , Drug Resistance, Microbial , Electrophoresis, Gel, Pulsed-Field , Humans , Ireland , Microbial Sensitivity Tests , Refrigeration , Salmonella/growth & development , Serotyping
SELECTION OF CITATIONS
SEARCH DETAIL
...