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1.
J Acoust Soc Am ; 142(5): EL473, 2017 11.
Article in English | MEDLINE | ID: mdl-29195440

ABSTRACT

Forecasting ambient noise levels in the ocean can be a useful way of characterizing the detection performance of sonar systems and projecting bounds on performance into the near future. The assertion is that noise forecasting can be improved with a priori knowledge of source positions coupled with the ability to resolve closely separated sources in bearing. One example of such a system is the large aperture research array located at the South Florida Test Facility. Given radar and Automatic Identification System defined source positions and environmental information, transmission loss (TL) is computed from known source positions to the array. Source levels (SLs) of individual ships are then estimated from computed TL and the pre-determined beam response of the array using a non-negative least squares algorithm. Ambient noise forecasts are formed by projecting the estimated SLs along known ship tracks. Ambient noise forecast estimates are compared to measured beam level data and mean-squared error is computed. A mean squared error as low as 3.5 dB is demonstrated in 30 min forecast estimates when compared to ground truth.

2.
J Proteome Res ; 11(8): 4382-91, 2012 Aug 03.
Article in English | MEDLINE | ID: mdl-22742968

ABSTRACT

Proteomics aspires to elucidate the functions of all proteins. Protein microarrays provide an important step by enabling high-throughput studies of displayed proteins. However, many functional assays of proteins include untethered intermediates or products, which could frustrate the use of planar arrays at very high densities because of diffusion to neighboring features. The nucleic acid programmable protein array (NAPPA) is a robust in situ synthesis method for producing functional proteins just-in-time, which includes steps with diffusible intermediates. We determined that diffusion of expressed proteins led to cross-binding at neighboring spots at very high densities with reduced interspot spacing. To address this limitation, we have developed an innovative platform using photolithographically etched discrete silicon nanowells and used NAPPA as a test case. This arrested protein diffusion and cross-binding. We present confined high density protein expression and display, as well as functional protein-protein interactions, in 8000 nanowell arrays. This is the highest density of individual proteins in nanovessels demonstrated on a single slide. We further present proof of principle results on ultrahigh density protein arrays capable of up to 24000 nanowells on a single slide.


Subject(s)
Lab-On-A-Chip Devices , Protein Array Analysis/instrumentation , Diffusion , Humans , Protein Biosynthesis , Protein Interaction Mapping , Proteome/biosynthesis , Proteome/genetics , Proteomics , Silicon/chemistry
3.
J Acoust Soc Am ; 126(5): 2234-41, 2009 Nov.
Article in English | MEDLINE | ID: mdl-19894804

ABSTRACT

Recently, a method has been developed that exploits the correlation properties of the ocean's ambient noise to measure water depth (a passive fathometer) and seabed layering [M. Siderius et al., J. Acoust. Soc. Am. 120, 1315-1323 (2006)]. This processing is based on the cross-correlation between the surface noise and the echo return from the seabed. To quantitatively study the dependency between processing and environmental factors such as wind speed, measurements were made using a fixed hydrophone array while simultaneously characterizing the environment. The measurements were made in 2006 in the shallow waters (25 m) approximately 75 km off the coast of Savannah, GA. A Navy tower about 100 m from the array was used to measure wind speed and to observe the sea-surface using a video camera. Data were collected in various environmental conditions with wind speeds ranging from 5 to 21 ms and wave heights of 1-3.4 m. The data are analyzed to quantify the dependency of passive fathometer results on wind speeds, wave conditions, and averaging times. One result shows that the seabed reflection is detectable even in the lowest wind conditions. Further, a technique is developed to remove the environmental dependency so that the returns estimate seabed impedance.


Subject(s)
Acoustics , Environment , Models, Theoretical , Oceanography/methods , Seawater , Calibration , Oceans and Seas , Surface Properties , Wind
4.
J Acoust Soc Am ; 124(2): 841-50, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18681576

ABSTRACT

A coupled hydrodynamic-acoustic model describing acoustic propagation in a fluid containing multiple bubbles is proposed and applied to simulate noise generated by impacting water jets. The total pressure is decomposed into a "hydrodynamic" part and an "acoustic" part and computed using different schemes. The hydrodynamic pressure field is calculated independently using a generalized hydrodynamic model, and the pressure variations serve as sources in the wave equation for the acoustic pressure. A numerical algorithm developed to calculate wave propagation in an irregular region is used to account for the existence of the cavities. Noise generated by the impact of two cylindrical water jets is predicted. The computed near-field pressure is compared with the experimental data.


Subject(s)
Acoustics , Gases , Models, Theoretical , Noise , Water , Algorithms , Computer Simulation , Motion , Power Plants , Pressure , Reproducibility of Results , Sound Spectrography , Surface Properties , Time Factors
5.
J Acoust Soc Am ; 112(2): 481-8, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12186029

ABSTRACT

Range-time-frequency distributions of surf-generated noise were measured within the surf zone during the SandyDuck'97 experiment at Duck, NC. A 24-phone, 138-m, bottom-mounted, linear array located along a line perpendicular to the shore at a depth of 1 to 3 m recorded the surf-generated noise. Concurrent video measurements of the location, size, and time-evolution of the individual breaking waves directly above the array were made from a nearby 43-m tower. Source level spectra are obtained by using a modified fast field program to account for water column and geoacoustic propagation from the distributed source region to an individual hydrophone. The length, location, and orientation of the leading edge of breakers are tracked in time from rectified video images. It is observed that the source levels from spilling breakers are lower (approximately 5-10 dB) than those produced by plunging breakers that occurred during the same time period. Plunging breakers generated time-frequency signatures with a sharp onset while spilling breakers' signatures had a gradual low-frequency precursor. Range-time signatures of plunging breakers indicate a burst of acoustic energy while spilling breakers' signatures depict sound being generated over a longer time period with the source region moving with the breaking surface wave.

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