ABSTRACT
This study aimed to investigate the effect of using menbutone in addition to ammonium molybdate on liver enzymes in sheep naturally poisoned with copper. Merino lambs (n = 30), naturally poisoned with copper and which also had high liver enzyme levels, were divided into two groups, each with 15 lambs. The AM + MEN group received ammonium molybdate and menbutone and the AM group received only ammonium molybdate solution. Both groups received 1.7% ammonium molybdate solution (1 mL per 10 kg body weight [BW]) subcutaneously on 0, 2nd and 4th days of the study. Menbutone (Genabil®, Boehringer Ingelheim, Germany) was administered intramuscularly at a dose of 10 mg/kg BW on days 0 and 2, in addition to ammonium molybdate in the AM + MEN group. Blood samples were collected on days 0 and 7, and aspartate aminotransferase (AST), gamma-glutamyltranspeptidase (GGT) and creatinine levels were evaluated. Over 7 days, AST levels decreased from 351.04 ± 63.50 IU/L to 286.40 ± 55.68 IU/L in the AM group (P > 0.05) and from 425.00 ± 119.25 IU/L to 240.83 ± 29.62 IU/L in the AM + MEN group (P ≤ 0.05). GGT levels decreased from 121.16 ± 15.88 IU/L to 110.39 ± 10.13 IU/L in the AM group (P > 0.05) and 124.52 ± 15.50 to 98.60 ± 9.08 IU/L in the AM + MEN group (P ≤ 0.05). Based on these findings, the use of menbutone, in addition to ammonium molybdate, has significantly reduced the level of liver enzymes.
