[Development of methods for determining acrylamide in food products by gas-liquid chromatography].

The method of determination of acrylamide in various food (milk powder, potato chips, instant coffee) by gas-liquid chromatography after pre-bromination was developed. Studies have shown the possibility of using bromination of acrylamide to give it the necessary properties for better extraction, purification and detection. Also revealed the possibility of qualitative and quantitative determine a acrylamide in food by gas-liquid chromatography with detection by electron capture detector.

[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].

The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg.

[The role of changes in thrombocytic hemostasis in the pathokinesis of purulent surgical infection]. / Rol' izmenenii trombotsitarnogo gemostaza v patokineze gnoinoi khirurgicheskoi infektsii.

A study of 425 patients demonstrated that a pyodestructive process, located anywhere, affects platelet functional properties, resulting in the first place, in disorders of spontaneous cell disaggregation. Impaired platelet functional properties are conducive to the development of postoperative purulent and thromboembolic complications.

[Electrophoretic properties of a population of Escherichia coli cells studied by microelectrophoresis]. / Issledovanie élektroforeticheskikh svoistv populiatsii kletok Escherichia coli metodom mikroélektroforeza.

The technique of microelectrophoresis was used to measure the rate of E. coli cell motility under the action of a constant electric field, and the electrokinetic potential (xi-potential) was calculated from the experimental data. As was shown by analysing the results for a sample from 100 cells, the property varied within a wide range which exceeded several times the error of a single measurement. This variation was associated with the different electrosurface properties of microbial cell groups and is characteristic of a cell population. The empirical distribution of the parameter was shown to be consistent with the normal law. The results of determining the electrokinetic potential may statistically be processed with a computer, and the profile of an experimental curve will characterize the physico-chemical state of cells.

[Effect of interlok, remantadine and biotop on normal monocyte functional activity and in acute viral hepatitis]. / Vliianie interloka, remantadina i biotopa na funktsional'nuiu aktivnost' monotsitov v norme i proi ostrom virusnom gepatite.

The influence of interlok, biotop and remantadine on the functional activity of monocytes was determined with the use of the NBT test permitting the quantitative evaluation of this characteristic. In this study interlok was found to give promising results in the treatment of acute viral hepatitis, due both to its antiviral action and to the stimulation of the functional activity of monocytes. biotop proved to render a sharply pronounced stimulating effect on the functional activity of monocytes, thus enhancing antiviral resistance. Remantadine suppressed the functional activity of monocytes and increased their lesion by the virus, thus creating favorable conditions for virus persistence in monocytes.