[The evaluation of diagnostic effectiveness of algorithm of isolation and express identification of opportunistic enterobacteria using national chromogenic growth mediums].

The testing of specificity and diagnostic effectiveness was applied to new national chromogenic growth mediums. The algorithm was developed to isolate and express identify the clinically significant and sanitary demonstrative opportunistic enterobacteria using chromogenic growth mediums.

[Multiple antibiotic resistance of associative microflora during urogenital pathology].

Susceptibility of associative microflora isolated from patients with inflammatory diseases of urogenital tract was investigated. Etiologic structure of the diseases and cross-resistance to antibiotics of Escherichia coli, Staphylococcusaureus, and Klebsiella pneumoniae strains isolated from women with endocervicitis and men with urethritiswas assessed. Ciprofloxacin and gentamycin had the highest activity, whereas beta-lactam antibiotics were mildly active. Isolated strainswere resistant to macrolides, penicillines and imipenem. Main types of multidrug resistance to antibiotics were presented in strains circulated in Dagestan.

[Diagnostic effectiveness of nutrient media for the bacteriological detection of enterobacteria].

The effectiveness of new nutrient media intended for the differential isolation of pathogenic and opportunistic enterobacteria (SS agar), as well as for the preliminary identification of enterobacteria, was determined on a wide range the strains of the family Enterobacteriaceae. The comparative evaluation of SS agar and Ploskirev's bactoagar was carried out. The newly developed medium was shown to have advantage in its differentiating and inhibiting properties. The clinical trials of the media, carried out in this study, revealed high detection rate and exceedingly exact differentiation of lactose-negative lactose-positive enterobacteria, as well as 100% coincidence of the results obtained on the nutrient medium for the preliminary identification of enterobacteria and on laboratory-made control media.

[Microtest systems in the diagnosis of cholera]. / Ispol'zovanie mikrotest-sistem v diagnostike kholery.

Micro test systems for the biochemical identification of comma bacilli (MTS-V) and for a rapid determination of their groups according Heiberg (MTS-H) were designed and commissioned into medical practice. The micro test systems showed their advantages before the control media of Hiss and Bacterium Incubation Media at studying the museum-kept and freely-obtained vibrio strains as well as at studying the concomitant microflora and clinical materials taken from ill people and vibrio-carriers during the 1994 outbreak of cholera in Daghestan.

[Protection of mammalian genome from the mutagenicity of styrene and aniline using the extract from unabi(Zizyphus mill) fruit and a composite preparation]. / Zashchita genoma mlekopitaiushchikh ot mutagennosti stirola i anilina ékstraktom iz plodov unabi (Zizyphus mill) i skonstruirovannym kompozitsionnym preparatom.

The unabi (Zizyphus Mill) fruit extract as well as a composite preparation produced on its base in proportional mix with the extract of germinating wheat seed embryos were determined on antimutagenous activity in model experiments with laboratory animals. The specified gene-protected properties of the preparation were approved against the influence of work place environmental factors in chemical and textile industries.

[The development and testing of dried nutrient media for the demonstration of ureaplasmas]. / Razrabotka i ispytaniia sukhikh pitatel'nykh sred dlia indikatsii ureaplazm.

The results of the development of new culture media for the indication of ureaplasmas are presented. The media have been developed on the basis of the dried nutrient broth produced by the Research and Production Amalgamation "Nutrient Media". The composition of the media includes Russian-made ingredients ensuring the growth of ureaplasmas. The physico-chemical and biological characteristics of the media have been perfected. The clinical trial of the newly developed media has been carried out in the process of the examination of 280 pregnant women with the normal course of pregnancy, with the threat of pregnancy interruption and with complications of pregnancy.

[An evaluation of a nutrient medium for isolating and cultivating Helicobacter pylori]. / Otsenka pitatel'noi sredy dlia vydeleniia i kul'tivirovaniia Helicobacter pylori.

Selective Helicobacter agar containing the selective supplement and blood, adding ex tempore, for the isolation and cultivation of H. pylori was developed. The Helicobacter agar was studied with the use of 5 newly isolated H. pylori strains, 13 bacterial associated cultures, as well as 21 inoculated biopsy specimens of the gastric and duodenal mucosa of patients with peptic ulcer. The study revealed that Helicobacter agar ensured the growth of H. pylori and their isolation from clinical material. The positive results after the inoculation of the specimens of biopsy material on Helicobacter agar and control media was 85%. In addition, the study of Helicobacter agar showed that it also exhibited pronounced selective properties with respect to bacterial associations, not inhibiting the growth of Helicobacter organisms and retaining their main biological properties. It is possible to recommend Helicobacter agar for use in laboratory practice in diagnosing Helicobacter-associated diseases.

[Study of the phenomenon and substantiation of the mechanism of antimutagenesis under conditions of action of sodium fluoride]. / Issledovanie fenomena i obosnovanie mekhanizma antimutageneza v usloviiakh vozdeistviia ftoristym natriem.

Transplanted human amnion cells have been used in experiments differing in the regularity of the sodium fluoride and alpha-tocopherol action to determine a considerable antimutagenic efficiency of the mutagenic process modifier, the efficiency being dependent on the treatment variability.

[Standard dried nutrient medium for determining microorganism sensitivity to antibiotics]. / Standartnaia sukhaia pitatel'naia sreda dlia opredeleniia chuvstvitel'nosti mikroorganizmov k antibiotikam.

The possibility of standardization of the dry nutrient medium AGV by the content of calcium and magnesium ions was studied. For manufacturing the commercial lots of the medium it is necessary to use special batches of agar-agar. Biological assay with the use of standard strains is included into specification of the medium.

[Effect of R plasmids on the growth characteristics of Shigella sonnei and Escherichia coli]. / Izuchenie vliianiia R-plazmid na rostovye kharakteristiki Shigella sonnei i Escherichia coli.

Revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: E. coli 15-3 and Sh. sonnei 11-941 containing conjugative R plasmids differing in the set of the resistance markers. It was shown that the R plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. It was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of Sh. sonnei 11-941 and its plasmid variants was of the same order. Counting of the viable cells in mixed cultures of the plasmid-free strain of E. coli 15-3 and its plasmid variant on the complete nutrient medium revealed an insignificant increase (by 10 per cent) in the proportion of the bacteria carrying the plasmid after 6-hour growth (during early stationary growth phase) and later, up to 24 hours. The results of the study suggested that development of nutrient media for microbial strains containing R plasmids does not require additional cultivation conditions.

[Use of Ploskirev's medium with tetracycline for the bacteriological diagnosis of dysentery]. / Ispol'zovanie sredy Ploskireva s tetratsiklinom dlia bakteriologicheskoi diagnostiki dizenterii.

The Ploskirev medium with addition of tetracycline in an amount of 10 mu/ml was used for increasing the isolation frequency of dysenteric bacteria. 102 faeces samples from children with acute dysentery were tested. It was shown that the Ploskirev medium with tetracycline was elective and its use is advisable for isolation of antibiotic resistant Shigella.