ABCA7 and EphA1 Genes Polymorphisms in Late-Onset Alzheimer's Disease.

Large-scale genome-wide studies have revealed the role of several genes and their respective single-nucleotide polymorphisms (SNPs) in the pathophysiology of late-onset Alzheimer's disease (LOAD). Here, the frequencies of ABCA7 SNPs rs3764650 and rs4147929 and EphA1 SNP rs11771145 were assessed and compared in LOAD patients and healthy subjects. In a case-control study, 110 patients with LOAD (case) and 88 healthy unrelated age- and gender-matched individuals (control), both from Azeri descent, were enrolled. DNA was extracted from blood samples using the salting out method, and the genotyping was performed by RFLP-PCR for rs3764650, rs4147929, and rs11771145 polymorphisms. Electrophoresis was carried out on agarose gel. Sequencing was utilized for confirmation of the results. No differences were found in the frequencies of ABCA7 SNP rs3764650 and EphA1 SNP rs11771145 between healthy subjects and LOAD patients. However, a significant difference was revealed in the frequencies of AA (p = 0.042, OR = 0.150; 95%CI = 0.005-1.410) and GG (p = 0.009, OR = 1.716; 95%CI = 0.918-3.218) genotypes of ABCA7 SNP rs4147929 between the mentioned groups. This study showed that ABCA7 SNP rs4147929 might be a predisposing factor for LOAD. However, such an association was not found between ABCA7 SNP rs3764650 as well as EphA1 SNP rs11771145 and LOAD. These results must be confirmed in other ethnic groups.

Correction to: ABCA7 and EphA1 Genes Polymorphisms in Late-Onset Alzheimer's Disease.

The original version of this article unfortunately contained a mistake in the Authorgroup section. Author Azra Delpak's given name was misspelled as "Azar".

Association of MS4A6A, CD33, and TREM2 gene polymorphisms with the late-onset Alzheimer's disease.

Introduction: Alzheimer's disease (AD), which is a progressive neurodegenerative disorder, causes structural and functional brain disruption. MS4A6A, TREM2, and CD33 gene polymorphisms loci have been found to be associated with the pathobiology of late-onset AD (LOAD). In the present study, we tested the hypothesis of association of LOAD with rs983392, rs75932628, and rs3865444 polymorphisms in MS4A6A, TREM2, CD33 genes, respectively. Methods: In the present study, 113 LOAD patients and 100 healthy unrelated age- and gender-matched controls were selected. DNA was extracted from blood samples by the salting-out method and the genotyping was performed by RFLP-PCR. Electrophoresis was carried out on agarose gel. Sequencing was thereafter utilized for the confirmation of the results. Results: Only CD33 rs3865444 polymorphism revealed a significant difference in the genotypic frequencies of GG (P = 0.001) and GT (P = 0.001), and allelic frequencies of G (P = 0.033) and T (P = 0.03) between LOAD patients and controls. Conclusion: The evidence from the present study suggests that T allele of CD33 rs3865444 polymorphism is associated with LOAD in the studied Iranian population.

The use of mixer torque rheometry to study the effect of formulation variables on the properties of wet granulations.

Mixer torque rheometry was used to investigate the rheological behavior of wet granulations with different concentrations of drug, binder, and water. An experimental design was employed to systematically study the effects of the three formulation variables on the torque profiles of the wet masses over time. Under comparable conditions, increasing binder and water concentrations tended to produce higher wet mass consistencies. Friability of the dried granules was measured as an indication of the strength of the granules. A reduced quadratic model in terms of each of the three variables was found to satisfactorily predict granule friability. Granule friability decreased with increases in the binder level and increased slightly with increasing drug concentration. An inverse relationship was seen between granule friability and the amount of water added to the formulation, especially at lower drug concentrations. Mixer torque rheometry is a useful method for studying the properties of wet granulations when minimal amounts of drug are available for the development of a wet granulated formulation.