ABSTRACT
Vibronic excitations in molecules are key to the fundamental understanding of the interaction between vibrational and electronic degrees of freedom. In order to probe the genuine vibronic properties of a molecule even after its adsorption on a surface appropriate buffer layers are of paramount importance. Here, vibrational progression in both molecular frontier orbitals is observed with submolecular resolution on a graphene-covered metal surface using scanning tunnelling spectroscopy. Accompanying calculations demonstrate that the vibrational modes that cause the orbital replica in the progression share the same symmetry as the electronic states they couple to. In addition, the vibrational progression is more pronounced for separated molecules than for molecules embedded in molecular assemblies. The entire vibronic spectra of these molecular species are moreover rigidly shifted with respect to each other. This work unravels intramolecular changes in the vibronic and electronic structure owing to the efficient reduction of the molecule-metal hybridization by graphene.
ABSTRACT
The molecular donor tetraphenyldibenzoperiflanthene (DBP) forms coverage-dependent superstructures on Au(111). At submonolayer coverage, the molecules align parallel to each other. They arrange in row-like structures, which exhibit a nearly rectangular primitive unit cell. By contrast, the molecular monolayer is characterized by a herringbone-type DBP arrangement spanned by an almost square unit cell containing two molecules. Both superstructures occur simultaneously in a narrow coverage range close to completion of the molecular monolayer. The adsorbate-substrate interaction is similar to other physisorbed molecular films on Au(111), but differs for the two adsorption phases as inferred from the different modification of the Au(111) surface reconstruction. Structural properties were consistently probed in real and reciprocal space by scanning tunneling microscopy and low-energy electron diffraction, respectively.
ABSTRACT
Tensor fascia lata is utilized in the management of complex soft-tissue injuries and defects, but has not been described in the literature in the use of tissue interposition with resection of talocalcaneal middle facet coalitions. This article is a case presentation of a resection of a middle facet coalition with interposition of an allograft of tensor fascia lata. At 14 months postoperative follow-up, range of motion of the subtalar joint was noted to be 20 degrees, and without pain or crepitus. There was no radiographic evidence of degenerative changes in Chopart's joint. The patient returned to all routine and sports activities without pain. He was satisfied with the outcome of the procedure.
Subject(s)
Fascia/transplantation , Subtalar Joint/pathology , Subtalar Joint/surgery , Synostosis/surgery , Adolescent , Calcaneus/pathology , Calcaneus/surgery , Humans , Male , Subtalar Joint/physiopathology , Talus/pathology , Talus/surgery , Transplantation, HomologousABSTRACT
Papillary eccrine adenoma is a rare, slow-growing cutaneous tumor of sweat gland origin. It is a benign lesion that occurs most often in the distal extremities. Only 33 cases have been reported in the literature with few located in the distal lower extremity. There have been no cases reported in the podiatric literature. The clinical and surgical history of a case report of a papillary eccrine adenoma in a 35-year-old white male is presented.
Subject(s)
Adenoma, Sweat Gland/diagnosis , Adenoma, Sweat Gland/surgery , Heel , Sweat Gland Neoplasms/diagnosis , Sweat Gland Neoplasms/surgery , Adult , Biopsy, Needle , Follow-Up Studies , Humans , Magnetic Resonance Imaging , Male , Photomicrography , Skin Transplantation , Treatment OutcomeABSTRACT
Clinical data were collected on injury occurrence to members of the University of Michigan Marching Band over the course of one season including a bowl game appearance. Band members were asked to fill out forms at the time of injury to gather pertinent information. The majority of the injuries were self-limiting. Included in this study was a total of 179 injuries, of which 153 (85.5%) involved the lower extremities. The authors provided a one-season injury survey of a collegiate marching band and made recommendations that may help to reduce and prevent future band-related injuries.
Subject(s)
Leg Injuries/etiology , Adolescent , Adult , Female , Humans , Male , Music , Wounds and Injuries/etiologyABSTRACT
The six sulfhydryl groups in each subunit of the alanyl-tRNA synthetase of Escherichia coli react with sulfhydryl reagents with at least four different rates. One reacts very rapidly with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), and a second reacts somewhat less rapidly with this reagent. These two groups are required for transfer activity, which is lost in proportion to the extent of derivatization. Two other groups react more slowly, with a consequent loss of exchange activity. The remaining two sulfhydryl groups do not react with DTNB until the protein is denatured. The inactivations are reversed by dithiothreitol. Two sulfhydryl groups react with N-ethylmaleimide (NEM) and with a spin-label derivative of NEM. These reactions resemble the modification of two sulfhydryl groups with DTNB, in that they also inactivate the transfer reaction but not the ATP:PPi exchange. The two spin labels are incorporated at similar rates but are in very different environments, one highly exposed and one highly immobilized. These groups do not interact with Mn2+, which is bound to the enzyme in the absence of ATP.
Subject(s)
Alanine-tRNA Ligase/metabolism , Amino Acyl-tRNA Synthetases/metabolism , Escherichia coli/enzymology , Sulfhydryl Compounds/metabolism , Alanine-tRNA Ligase/antagonists & inhibitors , Binding Sites , Chemical Phenomena , Chemistry , Dithionitrobenzoic Acid/pharmacology , Electrochemistry , Electron Spin Resonance Spectroscopy , Ethylmaleimide/pharmacology , Manganese/metabolism , Spin Labels , Sulfhydryl Reagents/pharmacologySubject(s)
Adenosine Triphosphate/analogs & derivatives , Affinity Labels/pharmacology , Amino Acyl-tRNA Synthetases/antagonists & inhibitors , Periodic Acid/pharmacology , Ribonucleotides/pharmacology , Adenosine Triphosphate/pharmacology , Animals , Escherichia coli/enzymology , Fructose-Bisphosphate Aldolase/antagonists & inhibitors , Isoleucine-tRNA Ligase/antagonists & inhibitors , Kinetics , Lysine-tRNA Ligase/antagonists & inhibitors , Muscles/enzymology , Oxidation-Reduction , Phenylalanine-tRNA Ligase/antagonists & inhibitors , RabbitsSubject(s)
Acetates , Amino Acyl-tRNA Synthetases/metabolism , Magnesium/pharmacology , Cations , Enzyme Activation/drug effects , Escherichia coli/enzymology , Hydrogen-Ion Concentration , In Vitro Techniques , Isoleucine-tRNA Ligase/metabolism , Lysine-tRNA Ligase/metabolism , Phenylalanine-tRNA Ligase/metabolism , Quaternary Ammonium Compounds/pharmacology , Spermine/pharmacologyABSTRACT
The effect of pH on the properties of the partial reactions of arginyl-tRNA synthetase of E. coli has been investigated. V max of pyrophosphorolysis of arginyl adenylate has a pH optimum at pH 6.1, whereas V max of the transfer of arginine to tRNA has a pH optimum of 8.2. These values correlate with the pH optima of the ATP:PPi exchange and the overall esterification reaction, respectively. Only the pyrophosphorolysis reaction requires a divalent cation; transfer proceeds in the presence of EDTA. Inorganic pyrophosphate inhibits the transfer reaction to an extent independent of the concentration of tRNA; the maximum inhibition is a function of pH, corresponding to the relative rate of pyrophosphorolysis of the common intermediate compared with the rate of transfer. These results show that different groups on the enzyme participate in the rate-limiting steps of the two partial reactions and that these partial reactions have properties consistent with their participation in the overall esterification of arginine with tRNA.
Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Arginine-tRNA Ligase/metabolism , Diphosphates/pharmacology , Escherichia coli/enzymology , Hydrogen-Ion Concentration , KineticsABSTRACT
The rate of transfer of amino acid from enzyme-bound aminoacyl adenylate to tRNA has been compared with the rate of esterification of free amino acid. The approach of Lövgren et al. (Lövgren, T. N. E., Heinonen, J., and Loftfield, R. B. (1975) J. Biol. Chem. 250, 3854-3860) was used, with 14C in the aminoacyl adenylate and 3H in the free amino acid and with both the lysine and isoleucine systems of Escherichia coli. In both systems kinetic analyses show more rapid transfer from the preformed enzyme complex when interference by the back reaction with inorganic pyrophosphate was eliminated. Parallel experiments, in which the amount of enzyme complex was measured, confirmed that aminoacyl adenylate is an intermediate in both systems. No evidence was found for an alternative mechanism.
Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Escherichia coli/enzymology , Isoleucine-tRNA Ligase/metabolism , Lysine-tRNA Ligase/metabolism , Transfer RNA Aminoacylation , Adenosine Monophosphate/metabolism , Isoleucine/analogs & derivatives , Kinetics , Lysine/analogs & derivatives , RNA, Transfer/metabolismABSTRACT
The primary structure of tRNALys of E. coli was determined by use of [32P]-tRNA. The sequence is pGGGUCGUUAGCUCAGDDGGDAGAGCAGUUGACUmam5-s2-UUU-t6AApsiCAAUUGm7GXCGCAGGTpsiCGAAUCCUGCACGACCCACCA. No s4-U was detected in position 8. No other lysine tRNA was detected but the existence of another species has not been ruled out.
Subject(s)
Escherichia coli/analysis , Lysine , RNA, Transfer/analysis , Autoradiography , Base Sequence , RNA, Bacterial/analysis , RibonucleasesABSTRACT
The question whether aminoacyl-tRNA synthetases act in a stepwise or in a concerted mechanism has been investigated kinetically with the valine enzyme of Escherichia coli, which had been used in previous studies by others who concluded that the physiological mechanism is concerted. An exchange between aminoacyl-tRNA and tRNA, dependent upon AMP, was studied. PP-i inhibits this exchange completely in the presence of Mg2+ and AMP but in the absence of added Mg2+ or with dAMP as the nucleotide the inhibition by PP-i is only partial; this is compatible with a stepwise, not a concerted, reaction. Exchange of isotopically labeled substrates in a system at chemical equilibrium also shows effects of substrate concentrations on rates in agreement with the predictions of a stepwise mechanism.
Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Escherichia coli/enzymology , RNA, Transfer/metabolism , Valine-tRNA Ligase/metabolism , Adenosine Monophosphate , Adenosine Triphosphate , Binding Sites , Diphosphates/pharmacology , Kinetics , Magnesium/pharmacology , Mathematics , Protein BindingABSTRACT
We have analyzed the function of spermine in the aminoacylation of tRNA-Val by the valyl-tRNA synthetase of Escherichia coli. Our results indicate that Mg2+ is required for the aminoacylation reaction as well as for the ATP-PP-i exchange catalyzed by this enzyme. The apparent stimulation by spermine is a function of the tRNA used, which appears to contain bound cations even after dialysis against 10 minus 4 M EDTA. Higher concentrations of EDTA totally abolish spermine-stimulated esterification of tRNA-Val.
Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Edetic Acid/pharmacology , Escherichia coli/enzymology , Magnesium/pharmacology , Spermine/pharmacology , Valine-tRNA Ligase/metabolism , KineticsSubject(s)
Amino Acyl-tRNA Synthetases/metabolism , Adenosine Triphosphate , Amino Acyl-tRNA Synthetases/antagonists & inhibitors , Animals , Binding Sites , Carbon Radioisotopes , Diphosphates , Electrophoresis, Paper , Escherichia coli/enzymology , Evaluation Studies as Topic , Kinetics , Mathematics , Methods , Pancreas/enzymology , Phosphoric Diester Hydrolases , Protein Binding , RNA, Transfer , Ribonucleases , Snakes , Spectrophotometry, Ultraviolet , Time Factors , Transfer RNA Aminoacylation , Valine , VenomsABSTRACT
Microheterogeneity of rabbit muscle aldolase is caused by deamidation in vivo of an asparagine residue near the C-terminus of each subunit. Isotopic labeling of a peptide containing the asparagine residue at various time intervals before isolation of aldolase permits estimation of the half-time for the deamidation as about 8 days, which is about the time estimated for the half-life of the enzyme in vivo. It is concluded that the aldolase as genetically determined is a tetramer, designated alpha(4), that undergoes random deamidation to form alpha(3)beta, alpha(2)beta(2), and alphabeta(3) species as intermediates in the formation of beta(4), the species in which all of the specific asparagine has been deamidated. Isoelectric focusing data indicate that the subunits do not exchange appreciably in vivo.