ABSTRACT
This study was aimed for the evaluation of somatic cell count (SCC), physicochemical, and microbiological parameters during the end of lactation in the raw milk of Alpine and native Red goat breed. In the experiment, 102 milk samples from Alpine and native Red goats were included. Two different groups within the same breed were analyzed: a group consisting of animals in their first lactation and the second group consisting of animals from the fifth lactation. The milk samples were individually and daily collected during late lactation for three consecutive weeks, and milk fat, protein, lactose, SCC, and total bacteria with enterobacteria were assessed. Fresh milk of goats from late lactation period had a number of somatic cells (SC) within the expected value with log10 of 5.8-6.18 cells/ml for the compared groups. In both breeds, the total mesophilic bacteria were fewer in numbers, however, in the native Red goat, a larger population of such bacteria was enumerated. The number of coliforms and enterobacteria was below 100 cfu/ml. In the current study, we were able to show a significant difference among the studied breeds depending on lactation and season for fat (p = 0.002), but not for lactose and protein content. A positive correlation for total protein (TP), lactose, and fat as well as for lactose and SCC was found in the native Red goat breed. In the Alpine goat breed, a strong positive correlation (0.821**) was found for lactose and enterobacteria count (EC). In conclusion, these findings evaluate different goat milk parameters during late lactation period and provide an indirect measure to monitor goat mammary gland health for both breeds.
ABSTRACT
Tick-borne diseases pose a serious threat to human health in South-Eastern Europe, including Kosovo. While Crimean-Congo hemorrhagic fever (CCHF) is a well-known emerging infection in this area, there are no accurate data on Lyme borreliosis and tick-borne encephalitis (TBE). Therefore, we sampled and tested 795 ticks. Ixodes ricinus (n = 218), Dermacentor marginatus (n = 98), and Haemaphysalis spp. (n = 24) were collected from the environment by flagging (all from Kosovo), while Hyalomma marginatum (n = 199 from Kosovo, all from Kosovo) and Rhipicephalus bursa (n = 130, 126 from Albania) could be collected only by removal from animal pasture and domestic ruminants. Ticks were collected in the years 2014/2015 and tested for viral RNA of CCHF and TBE viruses, as well as for DNA of Borrelia burgdorferi sensu lato by real-time PCR. In Kosovo, nine ticks were positive for RNA of Crimean-Congo hemorrhagic fever virus and seven for DNA of B. burgdorferi s. l. None of the ticks tested positive for TBEV. CCHF virus was detected in one H. marginatum male specimen collected while feeding on grazing cattle from the Prizren region and in eight R. bursa specimens (five females and three males collected while feeding on grazing sheep and cattle) from the Prishtina region (Kosovo). B. burgdorferi s. l. was detected in seven questing ticks (four male and one female D. marginatus, two I. ricinus one female and one male) from the Mitrovica region (Kosovo). Our study confirmed that CCHF virus is circulating in Kosovo mainly in H. marginatum and R. bursa in the central areas of the country. B. burgdorferi s. l. was found in its major European host tick, I. ricinus, but also in D. marginatus, in the north of the Kosovo. In order to prevent the spread of these diseases and better control of the tick-borne infections, an improved vector surveillance and testing of ticks for the presence of pathogens needs to be established.
