ABSTRACT
Present study was aimed to evaluate the wound healing activity of aqueous extract of leaves and roots of Coleus aromaticus using excisional wound model in albino rats. The aqueous extracts were prepared using maceration method and were applied as 5% and 10% ointment. The wound healing activity of these extracts was compared with a standard drug povidone-iodine ointment. The healing tissue was also tested for tensile strength, hydroxyproline content and protein content. The histopathological examination of healing tissue was also performed. Ten percent ointment of aqueous extract of root showed complete epithelization after 12 days (p <0.01) and 5% ointment of leaf extract showed complete healing after 16 days (p <0.01).
Subject(s)
Coleus , Plant Extracts/pharmacology , Wound Healing/drug effects , Animals , Female , Male , Plant Leaves , Plant Roots , Rats , Rats, WistarABSTRACT
Present study was aimed to elucidate hypolipidemic effect of fresh Triticum aestivum (common wheat) grass juice (GJ) in experimentally induced hypercholesterolemia in rats and to investigate its role in cholesterol excretion. Hypercholesterolemia was induced experimentally in rats by including 0.75 g% cholesterol and 1.5 g% bile salts in normal diet for 14 days. Hypercholesterolemic rats were administered fresh Triticum aestivum GJ at the dose of 5 mL/kg and 10 mL/kg and the standard drug atorvastatin 0.02% w/v in 2% gum acacia suspension at the dose of 1 mg/kg for 14 days by gavage. Blood samples were collected after 24 h of last administration and used for estimation of lipid profile. Fecal cholesterol levels were estimated using standard methods. Fresh GJ administration at 5 mL/kg and 10 mL/kg resulted in dose dependent significant decline in total cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) levels in hypercholesterolemic rats. Further, in comparison to atorvastatin, GJ administration at the dose of 10 mL/kg resulted in comparable decrease of TC, LDL-C, TG and VLDL-C levels (p > 0.05). Fecal cholesterol excretion was significantly (p < 0.05) enhanced by Triticum aestivum GJ administration. Phytochemical analysis revealed the presence of flavonoids, triterpenoids, anthraquinol, alkaloids, tannins, saponins and sterols in fresh wheat grass juice. The results of present study revealed hypolipidemic effect of Triticum aestivum GJ in hypercholesterolemic rats by increasing fecal cholesterol excretion. Fresh GJ could have potentially beneficial effect in atherosclerosis associated with hyperlipidemia.
Subject(s)
Anticholesteremic Agents/administration & dosage , Beverages , Hypercholesterolemia/drug therapy , Plant Extracts/administration & dosage , Triticum , Administration, Oral , Animals , Anticholesteremic Agents/chemistry , Atorvastatin , Bile Acids and Salts , Cholesterol, Dietary/blood , Cholesterol, LDL/blood , Cholesterol, VLDL/blood , Disease Models, Animal , Dose-Response Relationship, Drug , Feces/chemistry , Female , Heptanoic Acids/pharmacology , Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology , Hypercholesterolemia/blood , Hypercholesterolemia/etiology , Male , Plant Extracts/chemistry , Pyrroles/pharmacology , Rats , Rats, Wistar , Time Factors , Triglycerides/bloodABSTRACT
OBJECTIVE: To study the hypolipidemic activity of fresh grass juice of Triticum aestivum in normal rats. MATERIALS AND METHODS: Freshly prepared Triticum aestivum grass juice was administered to normal rats at the dose of 5 ml/kg and 10 ml/kg orally once daily for 21 days. Blood samples were collected after 24 hours of last administration and used for estimation of lipid profile. Fresh grass juice was also subjected to preliminary phytochemical screening. RESULTS: Fresh grass juice administration produced dose related significant (P < 0.05) reduction in total chloesterol, triglycerides, low density lipoprotein-cholesterol and very low density lipoprotein-cholesterol levels in normal rats as compared to control. Preliminary phytochemical screening revealed presence of alkaloids, tannins, saponins and sterols in Triticum aestivum grass. CONCLUSION: The results of the present study lndicate hypolipidemic activity of fresh Triticum aestivum grass juice.
