Does feeding extruded linseed to dairy cows improve reproductive performance in dairy herds? An observational study.

Feeding n-3 fatty acids (FA) is often cited as a promising strategy to tackle impaired reproduction in dairy cows. However, the scientific literature shows conflicting results that may be explained by the nature of n-3 FA used, the amount supplemented and the timing of supplementation. In addition, designing a proper experimental design to study n-3 FA and reproduction is subjected to other difficulties such as the choice of the control diet or gaining enough statistical power. The objective of this retrospective observational study was to quantify the average effects of supplementing extruded linseed (EL), a feed rich in α-linolenic acid, to dairy cows on reproductive performances under field conditions in French commercial farms. Exposure measurement to EL feeding was particularly challenging as exact cow diets are not traced in farms. Therefore, to investigate the potential dose-effect relationship, we defined a proxy of EL intake per day by using deliveries of EL based feeds from 22 companies in the study period 2008-2015 in France. An artificial insemination (AI) was considered exposed only if the cow was supplemented with EL from the calving until 17 days after AI. Based on recommendations for EL use on the field, 4 exposures classes were created: [1-50] (n = 14,126 AIs), [50-300] (n = 88,261 AIs), [300-600] (n = 66,136 AIs), and [600-1500] (n = 28,287 AIs) g/cow/d. The reference population was composed of cows that did not receive any EL between calving until 17 days after AI within herds that were supplied, but not continuously during the study period (n = 226,795 AIs). Mean daily EL intake in exposed population was 337 g/cow/d (±239.4). Reproductive performance was studied on 423,605 AIs from 1096 herds and 158,125 cows using Cox models for days to first AI and days to conception, and logistic regression models for risk of return-to-service, adjusted for factors likely to influence the reproductive performance and for a herd random effect. Risk of return-to-service between 18 and 78 days after first and second AI did not differ between exposed and reference populations, Nevertheless, the effect on the days to first AI was higher with the lowest EL intake (HR: 1.14; 95% CI: 1.11, 1.17) than with higher EL intake levels (HR ranging from 1.06 to 1.07; 95% CI: 1.04, 1.09). Similarly, for the effect on the time from calving to conception from the lowest EL intake (HR: 1.19; 95% CI: 1.15, 1.23) compared to the higher EL intake levels (HR ranging from 1.08 to 1.11; 95% CI: 1.06, 1.14). This original large-scale epidemiological study provides new insights into the effects of feeding EL at a commercially sustainable level to dairy cows.

Effects of feeding extruded linseed on production performance and milk fatty acid profile in dairy cows: A meta-analysis.

The objectives of this study were to quantify the effects on production performance and milk fatty acid (FA) profile of feeding dairy cows extruded linseed (EL), a feed rich in α-linolenic acid, and to assess the variability of the responses related to the dose of EL and the basal diet composition. This meta-analysis was carried out using only data from trials including a control diet without fat supplementation. The dependent variables were defined by the mean differences between values from EL-supplemented groups and values from control groups. The data were processed by regression testing the dose effect, multivariable regression testing the effect of each potential interfering factor associated with the dose effect, and then stepwise regression with backward elimination procedure with all potential interfering factors retained in previous steps. This entire strategy was also applied to a restricted data set, including only trials conducted inside a practical range of fat feeding (only supplemented diets with <60 g of fat/kg of dry matter and supplemented with <600 g of fat from EL). The whole data set consisted of 17 publications, representing 21 control diets and 29 EL-supplemented diets. The daily intake of fat from EL supplementation ranged from 87 to 1,194 g/cow per day. The dry matter intake was numerically reduced in high-fat diets. Extruded linseed supplementation increased milk yield (0.72 kg/d in the restricted data set) and decreased milk protein content by a dilutive effect (-0.58 g/kg in the restricted data set). No effect of dose or diet was identified on dry matter intake, milk yield, or milk protein content. Milk fat content decreased when EL was supplemented to diets with high proportion of corn silage in the forage (-2.8 g/kg between low and high corn silage-based diets in the restricted data set) but did not decrease when the diet contained alfalfa hay. Milk trans-10 18:1 proportion increased when EL was supplemented to high corn silage-based diets. A shift in ruminal biohydrogenation pathways, from trans-11 18:1 to trans-10 18:1, probably occurred when supplementing EL with high corn silage-based diets related to a change in the activity or composition of the microbial equilibrium in the rumen. The sum of pairs 4:0 to 14:0 (FA synthesized de novo by the udder), palmitic acid, and the sum of saturated FA decreased linearly, whereas oleic acid, vaccenic acid, rumenic acid, α-linolenic acid, and the sums of mono- and polyunsaturated FA increased linearly when the daily intake of fat from EL was increased. In experimental conditions, EL supplementation increased linearly proportions of potentially human health-beneficial FA in milk (i.e., oleic acid, vaccenic acid, rumenic acid, α-linolenic acid, total polyunsaturated FA), but should be used cautiously in corn silage-based diets.