Efficacy of tulathromycin in the treatment of respiratory disease in pigs caused by Actinobacillus pleuropneumoniae.

The efficacy of a single dose of tulathromycin, a novel triamilide antimicrobial of the macrolide class, given at 2.5 mg/kg or 5 mg/kg bodyweight, or three daily doses of ceftiofur, given at 3 mg/kg bodyweight, was evaluated in pigs with respiratory disease induced experimentally with Actinobacillus pleuropneumoniae. On day 0, 100 pigs with clinical signs of respiratory disease were randomly assigned to groups of 25 pigs, which were treated with either saline, one of the doses of tulathromycin, or ceftiofur. The pigs' rectal temperatures and clinical scores for respiratory signs and general attitude were recorded daily until day 10. Animals withdrawn from the study for welfare reasons were recorded. On day 10, the animals remaining in the study were weighed, euthanased and examined postmortem. Three of the animals treated with saline and one of those treated with 2.5 mg/kg tulathromycin were withdrawn from the study, but none of those treated with 5 mg/kg tulathromycin or ceftiofur were withdrawn. The least squares mean bodyweight gains of the pigs treated with the antimicrobial agents were significantly (P<0.05) higher than that of the saline-treated group, and the least squares mean percentages of the total lung involvement and incidence of respiratory disease associated with A. pleuropneumoniae were significantly (P<0.05) lower, but there were no significant differences between the three groups of pigs treated with the antimicrobial agents.

Efficacy of topically administered doramectin against eyeworms, lungworms, and gastrointestinal nematodes of cattle.

OBJECTIVE: To evaluate efficacy of topically administered doramectin against eyeworms, lungworms, and gastrointestinal nematodes of cattle. ANIMALS: 400 cattle (20 cattle in each of 20 trials). PROCEDURE: Trials were conducted in North America; natural and experimentally induced infections were used. In each trial, cattle were allocated randomly to control (placebo [saline [0.9% NaCl] solution at 1 ml/10 kg of body weight] or untreated; n = 10) or doramectin-treated (500 microg/kg of body weight; 10) groups. Treatments were applied in a single passage along the midline of the back, from the withers to the tailhead. Cattle were euthanatized > or =14 days after treatment, and worm burdens were determined by use of standard techniques. RESULTS: Efficacy of doramectin was > or =95.3% against adults of Thelazia gulosa, T skrjabini, Dictyocaulus viviparus, Haemonchus contortus, H placei, Ostertagia lyrata, O ostertagi, Trichostrongylus axei, Bunostomum phlebotomum, Capillaria spp, Cooperia oncophora, C pectinata, C punctata, C spatulata, C surnabada, Nematodirus spathiger, Strongyloides papillosus, T colubriformis, Oesophagostomum radiatum, and Trichuris spp. Efficacy was 95.1% against fourth-stage larvae of D viviparus, H placei, O lyrata, O ostertagi, T axei, C oncophora, C punctata, C spatulata, C surnabada, N helvetianus, T colubriformis, O radiatum, and Trichuris spp. In addition, efficacy against inhibited fourth-stage larvae of O ostertagi and Ostertagia spp was > or =98.1%. CONCLUSIONS AND CLINICAL RELEVANCE: A single topical application of doramectin pour-on was efficacious against a broad range of nematode species in cattle.

Efficacy of a pour-on formulation of doramectin against lice, mites, and grubs of cattle.

OBJECTIVE: To determine effectiveness of a pour-on formulation of doramectin against Damalinia bovis, Haematopinus eurysternus, Linognathus vituli, Solenopotes capillatus, Chorioptes bovis, Sarcoptes scabiei, Hypoderma bovis, and Hypoderma lineatum. ANIMALS: Cattle of various ages with naturally acquired or artificial infestations with 1 or more species of lice, mites, or grubs. PROCEDURE: In 10 louse and 6 mite studies, cattle were treated with doramectin (500 microg/kg, topically) on day 0, and parasite counts were performed approximately weekly from days 0 to 35. In 6 grub studies, cattle expected to harbor Hypoderma spp were treated before emergence of warbles. After warbles began to emerge, they were counted every 2 weeks, and grubs were collected and identified by species. RESULTS: Burdens of D bovis, H eurystemus, L vituli, and S capillatus on doramectin-treated cattle were 0 by 28 days after treatment. Burdens of C bovis and S scabiei decreased to 0 in naturally infested cattle and approximately 0 in artificially infested cattle by day 14 to 15. In grub studies, 107 of 136 control cattle had warbles, whereas 2 of 136 doramectin-treated cattle had 1 warble each, which represented a cure rate of 98.5%. CONCLUSION AND CLINICAL RELEVANCE: One topical application of doramectin was highly efficacious against common species of lice, mites, and grubs known to affect performance, health, and appearance of cattle.

Merit of outliers for milk yield as indicators of accuracy of genetic evaluations of sires.

To determine whether the accuracy of the genetic evaluations of sires can be assessed by the presence of extreme daughter records, we studied herd-years with records from first-crop daughters of 217 Holstein bulls that were not sampled by artificial insemination (AI) organizations but that later entered AI. The presence of outliers for standardized milk yield was determined within herd-year. Outliers were defined as records exceeding 1.5 interquartile ranges below the 25th percentile or above the 75th percentile. Herd-years were separated into two groups based on whether or not an outlier daughter record was present for an AI bull that had initially been sampled through non-AI. Herd-years without daughter outliers from those bulls were divided into herd-years with 1) no daughter outliers from any bull, 2) only negative daughter outliers from other bulls, 3) only positive daughter outliers from other bulls, or 4) negative and positive daughter outliers from other bulls. Herd-years with daughter outliers from AI bulls initially sampled through non-AI were divided into herd-years with 1) only negative daughter outliers, 2) only positive daughter outliers, 3) positive daughter outliers from those bulls and negative daughter outliers from other bulls, or 4) both negative and positive daughter outliers. The relationship between the frequency of outlier classes and a change in the Modified Contemporary Comparison genetic evaluations (the difference between the last available second-crop evaluation and the next to the last first-crop evaluation) was examined with logistic regression. For AI bulls that were initially sampled through non-AI and having evaluations that decreased > or = 386 kg, 9% of herd-years had positive first-crop daughter outliers and negative daughter outliers from other bulls; 38% had no outliers. For bulls with evaluations that increased > or = 194 kg, comparable percentages were 2 and 53%.

Field efficacy of doramectin pour-on against naturally-acquired, gastrointestinal nematodes of cattle in North America.

Seven studies were conducted under field conditions in North America to evaluate the therapeutic efficacy of doramectin in a pour-on formulation at a dosage of 500 microg/kg (1 ml/10 kg) for cattle harboring naturally-acquired infections of gastrointestinal nematodes, including species of Haemonchus, Ostertagia, Trichostrongylus, Bunostomum, Cooperia, and Oesophagostomum. In each study, 40 to 100 cattle were randomly allocated to a saline- or doramectin-treated group in a tiered manner based on Day -7 bodyweight. On Day 0, the cattle received either saline or doramectin topically, according to their treatment group. Weather and safety observations were made following treatment. No adverse reaction to treatment was observed at any time during these studies. Fecal egg count (FEC) determinations were carried out on each animal on Days -7, 0, 7, 14, and 21. Reductions in FEC for the doramectin-treated animals compared to saline-treated cattle were > or = 96.0% by Day -7 and > or = 99.0% on Days 14 and 21 for each study. Across all studies regardless of weather conditions, the reduction by Day 21 for the doramectin-treated animals compared to saline controls was 99.7% (p < or = 0.0001) and compared to pretreatment levels in doramectin-treated cattle was 99.9% (p < or = 0.0001). Doramectin pour-on should provide a useful new treatment for controlling nematode parasites of cattle.

Usability for genetic evaluations of records from herds participating in progeny test programs of artificial insemination organizations.

Thirteen AI organizations provided identification of herds that participated in their progeny test programs in 1989 and 1990; 15% of those herds participated in programs of more than one AI organization, but only 2.6% participated in programs of more than two AI organizations. Of the 19,589 participating herds, 82 and 76% were enrolled in DHI test plans that were considered to be usable for genetic evaluations during 1991 and 1992. For herds that had participated in AI progeny test programs, mean percentages of usable records were 77% in 1991 and 78% in 1992; the mean percentages of usable records for nonparticipating herds were 62% in 1991 and 60% in 1992. Participating herds had larger mean herd sizes, higher means and standard deviations of milk yields, younger cows, and a lower percentage of registered cows than did nonparticipating herds. Analysis of variance was used to explain the variation in the percentage of records that were usable for genetic evaluations. Herds that participated in AI progeny test programs or that had smaller herd sizes, higher mean milk yields, younger cows, or larger percentages of registered cows had higher percentages of records that were usable for genetic evaluations. Improved usability of records for genetic evaluations would increase the efficiency of AI progeny testing, and consideration of herd characteristics associated with higher percentages of usable records should aid AI organizations in evaluating prospective herds for progeny test programs.

Relationship of yield during early lactation and days open during current lactation with 305-day yield.

To measure and to partition the effect of pregnancy on yield, the relationships among milk, fat, and protein yields during early lactation, current days open, and 305-d yields were investigated using sample day records of 247,310 Holstein cows. The model included fixed effects of calving herd-year-season, calving age, and days open; the continuous variable of early cumulative yield to 80, 100, 120, or 140 d; and a random residual effect. As days open during first lactation increased from 30 to 100 d, 305-d milk yield increased by 876 kg; as days open increased from 100 to 200 d, milk yield increased by only 172 kg. The impact of current days open was greater on second lactation than on first; the difference in 305-d milk yield between cows open 40 and 290 d was 1199 kg for first lactation and 1613 kg for second lactation. If early yield to 120 d was included in the model, the corresponding difference was reduced to 860 kg for first lactation and 1001 kg for second lactation. Inclusion of early yield in the model reduced regression coefficients for days open during first lactation by 22% for 80-d yield, 24% for 100-d yield, 27% for 120-d yield, and 30% for 140-d yield and by 31, 35, 38, and 41%, respectively, for second lactation. Statistical models to derive adjustment factors should account for early lactation yield so that those factors can remove effects of pregnancy but not correlations between yield and fertility caused by early yield.

Consideration of percentage of milk shipped for calculation of total lactation yields from various morning and evening plans of milk sampling.

Milk yield recorded on DHI test day was compared with data on milk shipped from Texas and Minnesota herds for an innovative DHI test plan referred to as alternate a.m.-p.m. without a timer. Controls were yields for test day and for milk shipped from official DHI herds in Texas, Illinois, Minnesota, and several northeastern US states. Herd milk yield for a test day as a percentage of milk shipped was considered to be an indicator of the accuracy of the DHI recording plans. Mean percentage of milk shipped was 103 for all plans and regions. When herd test days with missing values were excluded, the percentage of herd test days within 96 to 110% of milk shipped were 77 for Texas and 82 for Minnesota innovative plans and 82 for Texas, 82 for Minnesota, 79 for Illinois, and 81 for northeastern official plans. Analysis indicated that the percentage of milk shipped was consistent across herd sizes, data source, and milk yield. Eight hypothetical testing plans were examined with or without adjustment of lactation yields for percentage of milk shipped. Estimates of variance components of lactation milk yields were computed and compared using a multitrait animal model. Adjustment of records for percentage of milk shipped would decrease mean milk yields by 3%, could result in better estimates of actual milk produced, but would have little effect on accuracy of genetic evaluations.

Age and seasonal effects on Holstein yield for four regions of the United States over time.

Multiplicative factors to adjust Holstein yield for age and season of calving were calculated and analyzed from 20 data files for region and time. Regions were 1) California, 2) Minnesota and Wisconsin, 3) New York and Pennsylvania, and 4) six southern states (Texas, Louisiana, Mississippi, Alabama, Georgia, and Florida). Time periods were calving years of 1964 to 1968, 1971 to 1975, 1976 to 1980, 1981 to 1985, and 1986 to 1990. The same statistical model was used that had been used to derive current USDA factors from 1964 to 1968 data. The number of lactation records for each data file ranged from 81,394 to 2,238,201 and increased with time. Effect of calving season on milk yield generally decreased with time; the largest decrease was for the southern region. Effect of calving age also generally decreased for recent data, and the largest decreases were for California. Updated factors to adjust yield for calving age and season are indicated for some regions.

Records contributing to national genetic evaluations by year, registration status, herd size, and region.

Lactation records from official DHIA were screened for usability in computing USDA-DHIA genetic evaluations. Counts of usable lactation records by state, registration status, and year were examined. Number of cows enrolled in official record-keeping plans of DHIA was the denominator for calculation of percentage of official DHIA records usable in genetic evaluations for state-years. Percentage of official records usable for genetic evaluations had a small increase from 44% during 1968 to 49% during 1978, had a steep increase through 1988 (65%), and then showed little change during the last 2 yr. Number of usable records of registered cows for genetic evaluations increased from 604,570 during 1968 to 864,630 during 1990, which, nevertheless, represented a decline from 64% of all usable records during 1968 to only 42% during 1990. Effects of year, DHIA region, average herd size, and average percentage of registered cows were important for explaining variation in percentage of records usable for genetic evaluations for state-years. States with large average herd sizes and low percentages of dairy cows that were registered had low percentages of records usable for genetic evaluations. Increased official DHIA participation and improved sire identification increases the percentage of records usable for genetic evaluations and helps facilitate genetic improvement.

Estimates of genetic trend in an artificial insemination progeny test program and their association with herd characteristics.

Individual lactation records from Holstein cows in 3449 herds participating in an AI stud's young sire sampling program from 1971 to 1987 were used to characterize the sampling program and to estimate genetic merit and trend. Average genetic merit of cows in sampling program herds was consistently superior to the average genetic merit of cows in the US population. Genetic trend of sires of first-crop cows was 58 kg of milk and 1.5 kg of fat/yr from 1971 to 1978 and 176 kg of milk and 5.5 kg of fat/yr from 1979 to 1987. The average genetic merit of sires of first-crop cows born after 1983 was equivalent to or exceeded the genetic level of sires of other cows in the herd. Within-herd-year means and standard deviations of yield, genetic evaluation, and management traits (herd-year characteristics) were computed for a subset of 341 herds contributing first-crop daughters for at least 10 yr. The average of each herd-year characteristic during 10 or more years was used to predict within-herd genetic trend. Herd characteristics explained up to 51% of differences in within-herd genetic trends. Average sire genetic merit of daughters other than first-crop daughters accounted for up to 80% of the explained differences. Other herd characteristics suggested that herds with larger within-herd standard deviation milk yields, a larger number of young sires represented, younger cows, and greater percentage of cows sired by AI sires made greater genetic improvement. Results indicated that the average genetic merit of cows and the rate of within-herd genetic improvement are higher in herds that participate in a young sire sampling program.

Association of genetic defects with yield and type traits: the weaver locus effect on yield.

The association of recessive genetic disorders with yield and type traits was investigated. The frequency of a defective gene could be increased by selection if it is positively associated with selected traits, despite efforts to reduce it. Genetic defects considered were weaver in Brown Swiss and rectovaginal constriction and limber leg in Jerseys. Data sets for linkage analysis consisted of 245 sons of 9 carrier sires, 1036 sons of 16 carrier sires, and 557 sons of 10 carrier sires, respectively. Weaver carrier sons had higher producing daughters than noncarrier sons within all 9 sire families. Weaver carrier cows have an advantage of 673.6 kg milk and 26.0 kg fat and a disadvantage in rear legs score, indicating that the condition may not be completely recessive. Carriers of the other defect genes have no advantage for milk production, are scored lower for pelvic angle, and limber leg carriers have more desirable udders. Estimates of defect gene frequencies in 264,000 Jersey cows show a decrease over time for rectovaginal constriction and limber leg; in 97,723 Brown Swiss cows, frequency of the weaver gene increased over time. Gene frequencies in daughters of the youngest sires were 5.48, 2.13, and 8.89%, respectively. Consistently higher yield evaluations of weaver carrier sons within each sire family, large advantage in production of weaver carrier cows, and increasing gene frequency over time indicate that a chromosome segment with major effect on yield is tightly linked to weaver in Brown Swiss.