Subject(s)
Diabetes Mellitus, Experimental/chemically induced , Hypolipidemic Agents/toxicity , Islets of Langerhans/drug effects , Xanthurenates/toxicity , Animals , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Experimental/pathology , Disease Models, Animal , Glucose Tolerance Test , Insulin/metabolism , Insulin Secretion , Islets of Langerhans/metabolism , Islets of Langerhans/pathology , Mice , Rabbits , RatsABSTRACT
Histochemical pseudoisocyanin and immunofluorescent methods were employed to investigate the level of insulin in cultivated pancreatic islet tissue (PIT) using two techniques: 1) staining of isolated PIT, cultivated on mica sheets; 2) staining of cultivated PIT sections. Stained sections ensure better results of microscopic and photometric investigations. On the whole, the most optimum method for histochemical analysis of the state of cultivated PIT is the combination of the above two techniques.
Subject(s)
Insulin/metabolism , Pancreas/metabolism , Animals , Animals, Newborn , Culture Techniques/methods , Fluorescent Antibody Technique , Histocytochemistry/methods , Quinolines , Staining and Labeling/methods , SwineABSTRACT
Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.
Subject(s)
Diabetes Mellitus, Experimental/pathology , Insulin/analysis , Islets of Langerhans/analysis , Animals , Fluorometry , In Vitro Techniques , Islets of Langerhans/pathology , Microscopy, Fluorescence , Rats , Rats, Inbred StrainsSubject(s)
Leukocyte Count/instrumentation , Urine/cytology , Adolescent , Adult , Aged , Clinical Trials as Topic , Emergencies , Female , Humans , Male , Middle AgedABSTRACT
Administration of a nondiabetogenic complex-former-sodium diethyldithiocarbamate (SDEDTC) to rabbits prevented the appearance in beta-cells of the insular apparatus of histological and ultramicroscopic changes with the subsequent administration of a potent diabetogenic agent-ditisone. SDEDTC blocked the insular zinc and prevented ditisone from forming in the islets a complex with this metal producing a destructive action of beta-cells.
