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Probl Endokrinol (Mosk) ; 33(6): 49-51, 1987.
Article in Russian | MEDLINE | ID: mdl-3325972

ABSTRACT

Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.


Subject(s)
Diabetes Mellitus, Experimental/pathology , Insulin/analysis , Islets of Langerhans/analysis , Animals , Fluorometry , In Vitro Techniques , Islets of Langerhans/pathology , Microscopy, Fluorescence , Rats , Rats, Inbred Strains
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