ABSTRACT
The investigation of microkinetics of 90Sr, 137Cs and 32P penetration into erythrocytes of dog's blood was made. It was shown that processes of penetration of 90Sr and 32P are monomolecular. The slow stage is monomolecular destruction of complexes of 90Sr and 32P with plasma components. The process of 137Cs penetration is bimolecular. The slowest stage of 137Cs penetration is adsorption on the surface of erythrocytes.
Subject(s)
Blood Cells/metabolism , Cesium Radioisotopes/blood , Phosphorus Radioisotopes/blood , Plasma/metabolism , Strontium Radioisotopes/blood , Animals , Dogs , Female , Male , Time FactorsABSTRACT
Decreased 2-deoxyglucose (2-DOG) uptake is well described in the neutrophils of patients with glycogen storage disease type 1b (GSD 1b). We report a patient with GSD 1b who presented with a normal antenatal and perinatal 2-deoxyglucose uptake that showed a slow regression during the first months of life. These indicate limitations of 2-deoxyglucose uptake in the diagnosis of GSD 1b. While it appears that low uptake rate below 0.25 nmol/min in 10(6) cells is of significance, normal uptake does not rule out the presence of the disease. It seems that antenatal diagnosis of GSD 1b cannot be made by measurement of 2-deoxyglucose uptake in the fetal neutrophils.
Subject(s)
Glucose/metabolism , Glycogen Storage Disease Type I/metabolism , Aging/metabolism , Deoxyglucose , Glycogen Storage Disease Type I/diagnosis , Glycogen Storage Disease Type I/enzymology , Humans , In Vitro Techniques , Infant , Infant, Newborn , Liver/enzymology , Liver/metabolism , Male , Neutrophils/metabolismABSTRACT
During development of transmitted solid hepatoma 22 pools of purine metabolites were studied as well as the rate of 3H-hypoxanthine and inosine incorporation was measured in acid soluble fraction of liver tissue and erythrocytes of mice C3HA as well as of the tumor tissue. Specific alterations in metabolism of adenine and guanine nucleotides and of their derivatives were detected at various steps of the hepatoma development in the tissues studied. Specific interaction of the hepatoma cells with host tissues appears to be realized via the pool of purine nucleotides.
Subject(s)
Erythrocytes/metabolism , Liver Neoplasms, Experimental/metabolism , Liver/metabolism , Purines/metabolism , Adenine Nucleotides/metabolism , Adenosine Triphosphate/metabolism , Animals , Guanine Nucleotides/metabolism , Hypoxanthines/metabolism , Inosine/metabolism , Male , Mice , Mice, Inbred C3H , Pyrimidines/metabolismABSTRACT
Utilization of ion-air reagents in a reversed-phase chromatographic system allows solving a number of problems related to the separation of purine and pyrimidine derivatives. Simultaneous analysis of nucleotides, nucleosides and their bases was carried out by acetonitrile gradient elution using tetrabutyl ammonium phosphate as a counterion in the mobile phase. Besides, optimal conditions were selected for isocratic separation of adenine nucleotides and their metabolites. Furthermore, isocratic separation of certain purines and pyrimidines was achieved by modifying the stationary C18-phase with pentane- and heptane sulphonic acids.
Subject(s)
Chromatography, High Pressure Liquid/methods , Purines/isolation & purification , Pyrimidines/isolation & purificationABSTRACT
Simultaneous separation of the main ribonucleotides, nucleosides and bases was carried out using tetrabutyl ammonium phosphate (TBA) hetaerons with a reversed-phase (C18) packing material. Chromatographic properties of purine and pyrimidine metabolites in radially compressed NovaPak C18 system was studied at various concentrations of acetonitrile and TBA in the mobile phase. The optimal conditions for gradient separation of nucleotides, nucleosides and bases were evaluated. The procedure was used for analysis of blood from healthy persons and patients with acute myocardial infarction.
Subject(s)
Chromatography, High Pressure Liquid/methods , Purine Nucleosides/analysis , Purine Nucleotides/analysis , Pyrimidine Nucleosides/analysis , Pyrimidine Nucleotides/analysis , Animals , Humans , Mice , Myocardial Infarction/blood , Purine Nucleosides/blood , Purine Nucleotides/blood , Pyrimidine Nucleosides/blood , Pyrimidine Nucleotides/blood , Thymus Gland/analysisABSTRACT
A method is described for the simultaneous analysis of twelve major nucleotides, certain biochemically essential nucleosides and nitrogen bases. The separation was achieved using an ion-pair reversed-phase high-performance liquid chromatographic (HPLC) system with a radially compressed NovaPak C18 cartridge and tetrabutylammonium phosphate (TBA) as a mobile phase modifier under acetonitrile gradient conditions. The effects of TBA and acetonitrile on the retention of major nucleotides were studied. The retention of nucleotides increased significantly with an increase in the TBA concentration from 0 to 2 mM and remained constant up to 5 mM TBA. The logarithm of the capacity factor decreased in proportion to the concentration of acetonitrile in the mobile phase. The separation of nitrogen bases, nucleosides and some of their deoxy forms was performed on an isocratic ion-pair reversed-phase HPLC system with pentanesulphonic and heptanesulphonic acid using a mu Bondapak C18 column. The described chromatographic procedures were applied to the separation of various biological extracts.
Subject(s)
Nucleosides/analysis , Ribonucleotides/analysis , Animals , Chromatography, High Pressure Liquid , Humans , Liver/analysis , Lymphocytes/analysis , Mice , Mice, Inbred C3H , Thymus Gland/analysisABSTRACT
The authors explored the statics of Ca2+ and Sr2+ sorption from a saline, imitating the salt (mineral) composition of the blood plasma, and from blood plasma. As the plasma concentration was raised, there was a consistent decrease in ion sorption of KU = 2 X 8 cationite characteristic for M2+ complex formation in solution. Conventional constants for formation of complex compounds and mole proportions (%) of free (M2+) and complex forms (MLx-) metals were calculated. The time course of calcium and strontium sorption on KU = 2 X 8 ion-exchange resin from saline and blood plasma was studied. It was discovered that sorption from saline proceeded according to the externally diffusion kinetics type, whereas sorption from plasma according to the mixed type of the ion-exchange kinetics. The kinetic ratios were calculated for the indicated versions of sorption experiments. The mechanisms of sorption found in model experiments were extrapolated to dog experiments with blood decalcination, which supported the adequacy of experimental hemosorption under consideration.
Subject(s)
Calcium/blood , Cation Exchange Resins , Hemoperfusion/methods , Ion Exchange Resins , Strontium/blood , Animals , DogsABSTRACT
A case of hypernephroma is presented in which the initial finding was heavy bleeding from a vaginal tumor. Fewer than 90 cases of genital metastases in males or females have previously been reported.
Subject(s)
Adenocarcinoma/diagnosis , Kidney Neoplasms/diagnosis , Vaginal Neoplasms/secondary , Adenocarcinoma/secondary , Diagnosis, Differential , Female , Humans , Kidney Neoplasms/pathology , Vaginal Neoplasms/diagnosisABSTRACT
A method of cation metabolism on cationite KY=2X8 in the balanced-salt form was applied to the study of sorption of Mg2+, Ca2+ and Sr2+ ions from physiological solutions and solutions containing blood plasma of dogs and physiological saline in different ratio. Balance constants, which permitted to determine mol fractions (or the content percentage), of "free" and "bound" ions of the corresponding metals were calculated from the experiments.
