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1.
Tsitol Genet ; 44(4): 16-22, 2010.
Article in Russian | MEDLINE | ID: mdl-20722281

ABSTRACT

The level of gene polymorphism of 10 microsatellite loci in 27 American Pit Bull Terrier dogs which have a high value of the percentage of blood and inbreeding coefficient achieved 43.8% was studied. The excess of heterozigosity over expected for this level of inbreeding coefficient was established. Suggestion that the high level of heterozigosity is the result of the selection against homozygotes was made.


Subject(s)
Dogs/genetics , Inbreeding , Microsatellite Repeats/genetics , Polymorphism, Genetic , Animals , Animals, Inbred Strains , Genetic Markers , Heterozygote , Pedigree
2.
Mikrobiol Z ; 68(4): 70-8, 2006.
Article in Russian | MEDLINE | ID: mdl-17100331

ABSTRACT

A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.


Subject(s)
Influenza A Virus, H5N1 Subtype , Influenza in Birds/virology , Polymerase Chain Reaction/methods , Amino Acid Sequence , Animals , Birds , Influenza A Virus, H5N1 Subtype/genetics , Influenza A Virus, H5N1 Subtype/isolation & purification , Influenza A Virus, H5N1 Subtype/pathogenicity , Molecular Sequence Data , RNA, Viral/genetics , Reproducibility of Results , Sensitivity and Specificity , Virulence
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