1.
Klin Lab Diagn
; 59(11): 50-3, 2014 Nov.
Article
in Russian
| MEDLINE
| ID: mdl-25850248
ABSTRACT
The article presents data concerning analysis of bioassays under suspicion of influenza A(H1N1)pdm09. The technique of back transcription polymerase chain reaction and sequencing was applied. The separation of native influenza virus A was implemented using passaging through developing chick embryos.
Subject(s)
Hemagglutinins, Viral/genetics , Influenza A Virus, H1N1 Subtype/genetics , Influenza, Human/virology , RNA, Viral/isolation & purification , Animals , Chick Embryo , Hemagglutinins, Viral/isolation & purification , High-Throughput Nucleotide Sequencing , Humans , Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza A Virus, H1N1 Subtype/pathogenicity , Influenza A Virus, H1N1 Subtype/ultrastructure , Influenza, Human/genetics , Influenza, Human/pathology , Microscopy, Electron , RNA, Viral/genetics
2.
Klin Lab Diagn
; (3): 41-4, 2013 Mar.
Article
in Russian
| MEDLINE
| ID: mdl-23808026
ABSTRACT
The external and internal control samples are used in case of polymerase chain reaction application to ensure validity of results. The external control samples are used to establish operability of reagents included into diagnostic kit. The internal control samples make it possible to check not only all the stages of reaction but also the process of amplification in each individual test tube with reaction mixture.
Subject(s)
DNA/isolation & purification , Reference Standards , Reverse Transcriptase Polymerase Chain Reaction/methods , DNA/chemistry , Humans , Reverse Transcriptase Polymerase Chain Reaction/standards
3.
Voen Med Zh
; 328(9): 57-63, 2007 Sep.
Article
in Russian
| MEDLINE
| ID: mdl-18154055
