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1.
Ter Arkh ; 95(11): 958-964, 2023 Dec 22.
Article in Russian | MEDLINE | ID: mdl-38158952

ABSTRACT

AIM: The search for etiopathogenetic agents to prevent the development of severe and extremely severe COVID-19 remains relevant. A placebo-controlled randomized clinical trial was conducted to evaluate the efficacy and safety of the antibody-based biological drug (Raphamin). MATERIALS AND METHODS: 785 outpatients 18-75 y.o. with laboratory confirmed mild COVID-19 were included within 24 hours from the disease onset. 771 patients were randomized to the group Raphamin (n=382) and the Placebo group (n=389). The study drug/placebo was prescribed for 5 days. The rate of progression to a more severe degree of COVID-19 by day 28 as well as the time to sustained clinical recovery and the frequency of hospitalization were evaluated. Safety was assessed taking into account adverse events, vital signs and laboratory parameters. RESULTS: The number of cases of progression to a more severe degree of COVID-19 in participants receiving Raphamin was 59 (15.5%) [52 (14.6%)] versus placebo - 89 (22.9%) [85 (23.7%)], ITT and [PP] analysis data are presented. The odds ratio between groups was OR=0.6157 [OR=0.5494], 95% confidence interval 0.4276-0.8866 [0.3750-0.8048], which meant a reduction in the chance of progression to a more severe degree by 38.4% [45.1%] or 1.48 [1.62] times; p=0.0088 [p=0.0019]. The time to sustained recovery in the Raphamin group was 4.5±2.4 [4.6±2.4] days, versus placebo - 5.8±4.7 [6.0±4.8] days; p=0.0025 [p=0.0036]. No adverse events with a certain relationship were registered. CONCLUSION: Raphamin reduces the risk of progression to a more severe degree of the COVID-19 and significantly shortens the duration of clinical symptoms.


Subject(s)
COVID-19 , Humans , SARS-CoV-2 , Treatment Outcome , Hospitalization , Double-Blind Method
2.
Bull Exp Biol Med ; 164(1): 75-79, 2017 Nov.
Article in English | MEDLINE | ID: mdl-29119394

ABSTRACT

The effects of bone marrow multipotent mesenchymal stromal cells and their secretion products on the subpopulation composition of thymic and splenic lymphocytes were studied in female Wistar rats with experimental chronic inflammatory process in the internal genitals. Stromal cells and medium conditioned by these cells in different administration routes (intravenous or lymphotropic injection) produces different modulating effect on blood leukocyte count and on subpopulation composition of the splenic and thymic lymphocytes. The most manifest anti-inflammatory effect was observed after lymphotropic injection of multipotent mesenchymal stromal cells creating a high concentration and long persistence of the factors produced by these cells in the focus of inflammation.


Subject(s)
Mesenchymal Stem Cells/metabolism , Spleen/pathology , Thymus Gland/pathology , Vaginitis/therapy , Animals , Bone Marrow Cells/metabolism , Cells, Cultured , Female , Lymphocyte Count , Mesenchymal Stem Cell Transplantation , Rats, Wistar , Spleen/immunology , T-Lymphocytes/physiology , Thymus Gland/immunology , Vaginitis/immunology
3.
Bull Exp Biol Med ; 161(1): 150-4, 2016 May.
Article in English | MEDLINE | ID: mdl-27270930

ABSTRACT

We studied the effect of a biomedical cell product (bone marrow multipotent mesenchymal stromal cells and products secreted by these cells in conditioned medium) on subpopulation composition of lymphoid cells of central and peripheral lymphoid organs of Wistar rats under normal conditions. Changes in the subpopulation composition of lymphoid organs depended on the route of administration of biomedical cell product.


Subject(s)
Lymph Nodes/cytology , Spleen/cytology , Thymus Gland/cytology , Administration, Intravenous , Animals , Cells, Cultured , Culture Media, Conditioned , Female , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/metabolism , Rats, Wistar
4.
Bull Exp Biol Med ; 160(1): 28-31, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26608379

ABSTRACT

We studied the effect of dyslipidemia induced by poloxamer 407 (300 mg/kg twice a week for 30 days) on cellular composition of the spleen and splenocyte lysosomes in mice. Changes in blood lipid profile included elevated concentrations of total cholesterol, aterogenic LDL, and triglycerides most pronounced in 24 h after the last poloxamer 407 injection; gradual normalization of lipid profile was observed in 4 days (except triglycerides) and 10 days. The most pronounced changes in the spleen (increase in organ weight and number of cells, inhibition in apoptosis, and reduced accumulation of vital dye acridine orange in lysosomes) were detected on day 4; on day 10, the indices returned to normal. Cathepsin D activity in the spleen also increased at these terms. The relationship between changes in the cellular composition of the spleen and dynamics of serum lipid profile in mice in dyslipidemia caused by repeated administrations of relatively low doses of poloxamer 407 is discussed.


Subject(s)
Dyslipidemias/pathology , Poloxamer/toxicity , Spleen/pathology , Animals , Cathepsin D/analysis , Cholesterol/blood , Cholesterol, LDL/blood , Dyslipidemias/blood , Dyslipidemias/chemically induced , Lysosomes/drug effects , Lysosomes/ultrastructure , Male , Mice , Mice, Inbred CBA , Organ Size/drug effects , Spleen/drug effects , Spleen/enzymology , Spleen/ultrastructure , Triglycerides/blood
5.
Bull Exp Biol Med ; 159(6): 732-5, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26515173

ABSTRACT

We studied the response of the pineal gland and organs of the immune system to melatonin treatment in Wistar rats kept under conditions of abnormal illumination regimen. The animals were kept under natural light regimen or continuous illumination for 14 days and then received daily injections of melatonin (once a day in the evening) for 7 days. Administration of melatonin to rats kept at natural light cycle was followed by a decrease in percent ratio of CD4+8+ splenocytes and CD4-8+ thymocytes. In 24-h light with the following melatonin injections were accompanied by an increase in percent rate and absolute amount of CD4+8+ cells in the spleen, and a decrease in percent rate of CD11b/c and CD4-8+ splenocytes. In the thymus amount of CD4-8+ cells increased, and absolute number of CD4+25+ cells reduced. Melatonin significantly decreased lipofuscin concentration in the pineal gland during continuous light. Direction and intensity of effects of melatonin on parameters of cell immunity and state of the pineal gland were different under normal and continuous light conditions. It should be taken into account during using of this hormone for correction of immune and endocrine impairments developing during change in light/dark rhythm.


Subject(s)
Circadian Rhythm/drug effects , Immune System/drug effects , Lighting , Melatonin/pharmacology , Photoperiod , Pineal Gland/drug effects , Animals , Circadian Rhythm/physiology , Immune System/anatomy & histology , Immune System/physiology , Lighting/adverse effects , Lymphocytes/cytology , Lymphocytes/drug effects , Male , Pineal Gland/anatomy & histology , Pineal Gland/physiology , Rats , Rats, Wistar , Spleen/anatomy & histology , Spleen/drug effects , Spleen/physiology , Thymus Gland/anatomy & histology , Thymus Gland/drug effects , Thymus Gland/physiology
6.
Bull Exp Biol Med ; 159(1): 62-5, 2015 May.
Article in English | MEDLINE | ID: mdl-26033592

ABSTRACT

We compared the effectiveness of immunomodulators used in the treatment of patients with chronic salpingitis and oophoritis with or without changes in succinate dehydrogenase (SDH) activity in blood lymphocytes at incubation with the drug. Diurnal variations in individual reaction of SDH in blood lymphocytes to thymalin or ridostin were revealed. In the groups of women receiving ridostin or thymalin during the reaction of lymphocyte SDH to it, improvement of clinical laboratory and immunological parameters was observed in the majority of the patients and no effect was found in a lesser group of patients than in the groups treated with drugs during the absence of lymphocyte SDH reaction thereto. The timing of the presence of SDH reaction to drugs in the immunocompetent cells makes it possible to set the optimal daily regime of their application and to select a drug that would be most effective in each particular case.


Subject(s)
Drug Chronotherapy , Immunologic Factors/administration & dosage , Lymphocyte Subsets/drug effects , Oophoritis/drug therapy , RNA, Double-Stranded/administration & dosage , RNA, Fungal/administration & dosage , Salpingitis/drug therapy , Succinate Dehydrogenase/blood , Thymus Hormones/administration & dosage , Adolescent , Adult , Anti-Bacterial Agents/therapeutic use , Chronic Disease , Combined Modality Therapy , Cytoplasmic Granules/enzymology , Drug Therapy, Combination , Female , Humans , Immunologic Factors/pharmacology , Killer Cells, Natural/drug effects , Killer Cells, Natural/enzymology , Killer Cells, Natural/immunology , L-Lactate Dehydrogenase/blood , Lymphocyte Subsets/enzymology , Lymphocyte Subsets/immunology , Monocytes/drug effects , Monocytes/enzymology , Monocytes/immunology , Oophoritis/immunology , Oophoritis/therapy , Physical Therapy Modalities , Precision Medicine , RNA, Double-Stranded/pharmacology , RNA, Fungal/pharmacology , Salpingitis/immunology , Salpingitis/therapy , Thymus Hormones/pharmacology , Treatment Outcome , Vitamins/therapeutic use , Young Adult
7.
Bull Exp Biol Med ; 158(6): 769-71, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25894775

ABSTRACT

We found the peculiarities of the effects of dipeptidyl peptidase IV inhibitor diprotin A on immunological parameters of lymphocytes in lymphoid organs in intact animals and in animals with experimental autoimmune process. In intact animals, diprotin A increased cellularity of the thymus and spleen, number of CD4+, CD8+, and CD4+8+ thymocytes and CD3+ splenocytes and reduced cellularity of lymph nodes and the number of antibody-forming cells in the spleen. Diprotin A administered against the background of autoimmune process, reduced thymus cellularity, number of antibody-forming cells in the spleen, and number of CD4 and CD4+8+ thymocytes in comparison with the corresponding parameters of autoimmune process.


Subject(s)
Autoimmunity/drug effects , Dipeptidyl-Peptidase IV Inhibitors/pharmacology , Oligopeptides/pharmacology , Animals , CD3 Complex/metabolism , CD4-Positive T-Lymphocytes/drug effects , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/drug effects , CD8-Positive T-Lymphocytes/immunology , Lymphocytes/drug effects , Lymphocytes/immunology , Male , T-Lymphocyte Subsets/drug effects , T-Lymphocyte Subsets/immunology
8.
Bull Exp Biol Med ; 158(4): 471-4, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25708328

ABSTRACT

We studied the chronodependent effect of IL-2 in the experimental model of immunodeficiency, cyclophosphamide-induced immunosuppression in mice. IL-2 in a dose of 100 U/ mouse was administered at 10.00 and 16.00 for 3 days after injection of cyclophosphamide. In contrast to the morning treatment with the cytokine, evening administration produced antiapoptotic effect on splenocytes and stimulated proliferation to a greater extent. This was accompanied by an increase in the number of CD4(+), CD25(+) and CD4(+)25(+) cells in the spleen to a level of intact mice. More pronounced effect of the evening mode of IL-2 administration on the proliferation and subpopulation composition of mouse spleen cells in the studied model can be associated with high blood level of CD25(+) cells at this time of the day.


Subject(s)
Cyclophosphamide/immunology , Immunologic Deficiency Syndromes/immunology , Immunosuppressive Agents/immunology , Interleukin-2/pharmacology , Spleen/cytology , Animals , Antibodies, Monoclonal , Apoptosis/drug effects , Cell Proliferation/drug effects , Fluorescein-5-isothiocyanate , Immunologic Deficiency Syndromes/chemically induced , Male , Mice , Mice, Inbred CBA , Statistics, Nonparametric , Time Factors
9.
Ross Fiziol Zh Im I M Sechenova ; 100(2): 201-14, 2014 Feb.
Article in Russian | MEDLINE | ID: mdl-25470897

ABSTRACT

UNLABELLED: Chronic social defeat stress leads to the development of mixed anxiety-depression state, which accompanied by immune deficiency in male mice. Paper aimed to study effects of ronkoleukin on the parameters of cellular immunity in the thymus and spleen and psychoemotional state in these animals. METHODS: Mixed anxiety/depression state was produced by chronic social defeat stress during 20 days in male mice. Roncoleukin (5000 ME/kg, i/p) and saline were chronically injected to depressive mice during 2 weeks without agonistic interactions. After this period subpopulations of lymphocytes in the thymus and spleen were studied in male mice. The animals were also studied in behavioral tests estimating the levels of communicativeness, anxiety and depressiveness. RESULTS: Roncoleukin decreases the number of lymphocytes in the thymus and spleen, and increased the number of lymphocytes in blood and thymus index. Medication increased per cent of CD4+8+ lymphocytes in the thymus and per cent of CD8+ and CD3+25- lymphocytes in the spleen. Roncoleukin induced anxiogenic, stimulative and antidepressive effects. CONCLUSION: Roncoleukin has small efficacy for treatment of immune suppression induced by chronic social defeat stress and has anxiogenic, stimulating and weak antidepressive effects.


Subject(s)
Antidepressive Agents/pharmacology , Anxiety/drug therapy , Depression/drug therapy , Immunologic Factors/pharmacology , Interleukin-2/pharmacology , Stress, Psychological/drug therapy , Animals , Anxiety/immunology , Anxiety/physiopathology , Behavior, Animal/drug effects , Depression/immunology , Depression/physiopathology , Immunity, Cellular/drug effects , Immunity, Innate/drug effects , Immunocompromised Host , Immunophenotyping , Injections, Intraperitoneal , Lymphocyte Count , Male , Mice , Mice, Inbred C57BL , Spleen/drug effects , Spleen/immunology , Stress, Psychological/immunology , Stress, Psychological/physiopathology , T-Lymphocyte Subsets/drug effects , T-Lymphocyte Subsets/immunology , Thymus Gland/drug effects , Thymus Gland/immunology
10.
Bull Exp Biol Med ; 158(1): 66-9, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25408522

ABSTRACT

The effects of dipeptidyl peptidase IV inhibitor sitagliptin on immunological parameters were studied in animals with experimental autoimmune process. The effects of the drug administered in preventive (before manifestation of autoimmune processes) and therapeutic (after manifestation of autoimmune process) modes were studied.


Subject(s)
Autoimmune Diseases/immunology , Dipeptidyl-Peptidase IV Inhibitors/pharmacology , Lymphocytes/immunology , Pyrazines/pharmacology , Triazoles/pharmacology , Animals , Drug Evaluation, Preclinical , Lymph Nodes/drug effects , Lymph Nodes/immunology , Lymphocytes/drug effects , Male , Mice, Inbred CBA , Sitagliptin Phosphate , Spleen/drug effects , Spleen/immunology , Thymus Gland/drug effects , Thymus Gland/immunology
11.
Bull Exp Biol Med ; 158(1): 42-5, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25403394

ABSTRACT

We studied the effects of melatonin on the status of immune organs and parameters of lipid metabolism in rats with alimentary obesity and parameters of lipid metabolism and immune status in Wistar rats kept on high-fat diet and receiving melatonin solution per os. Melatonin leveled the changes in blood and liver parameters of lipid metabolism, which was paralleled by normalization of cellular composition of immune organs. We conclude that melatonin can be a promising agent for the treatment of lipid metabolism and immune status disorders in alimentary obesity.


Subject(s)
Immunologic Factors/pharmacology , Lipid Metabolism/drug effects , Melatonin/pharmacology , Obesity/immunology , Spleen/drug effects , Animals , Cholesterol/blood , Diet, High-Fat/adverse effects , Drug Evaluation, Preclinical , Female , Leukocyte Count , Liver/drug effects , Liver/metabolism , Lymph Nodes/drug effects , Lymph Nodes/pathology , Lymphocytes/drug effects , Obesity/blood , Obesity/etiology , Rats, Wistar , Spleen/pathology , Triglycerides/blood
12.
Morfologiia ; 145(2): 26-30, 2014.
Article in Russian | MEDLINE | ID: mdl-25282821

ABSTRACT

The objective of this investigation was to determine the complex response of the pineal gland (PG) and of the organs of the immune system in Wistar rats in response to a violation of the illumination regime in the experiment. Animals were kept under natural light regime and continuous illumination for 14 days. After that rats were sacrificed and the mass of the body, PG, gonads, thymus and spleen was measured. Thymus and spleen cell subpopulations were determined by flow cytometry. The lipofuscin content in PG was determined by measuring an autofluorescence intensity in frozen tissue sections in the wavelength range of 505-545 nm using a confocal laser scanning microscope LSM 510 META (Carl Zeiss). The correlation analysis showed an increase in the amount and the change of sign and direction of relations between the indices of the state of PG and the immune system. This indicates the up-regulation of the intensity of inter-system relationships and the change of migration and differentiation vector of immunocompetent cells.


Subject(s)
Photoperiod , Pineal Gland , Spleen , Thymus Gland , Animals , Male , Pineal Gland/cytology , Pineal Gland/immunology , Rats , Rats, Wistar , Spleen/cytology , Spleen/immunology , Thymus Gland/cytology , Thymus Gland/immunology
13.
Bull Exp Biol Med ; 156(5): 642-4, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24770748

ABSTRACT

Neuroprotective effect of erythropoietin administered before ischemia has been previously demonstrated. The efficiency of erythropoietin administration after ischemia was not studied, though in case of success these protocols would be applied in clinical neurology. In our experiments on the model of transitory focal ischemia, erythropoietin was injected intraperitoneally during the early and delayed postischemic period (3 and 12 h). The size of the necrotic zone, neurological deficit, and the severity of brain edema were evaluated in 48 h. Injection of erythropoietin in 3 and 12 h after ischemia significantly reduced the size of necrosis (p = 0.0007 and p=0.0016, respectively), neurological deficit (p=0.0013 and p=0.0062, respectively), and brain edema (p=0.02 and p=0.0186, respectively). Injection of erythropoietin after transitory focal cerebral ischemia produced a pronounced neuroprotective effect.


Subject(s)
Erythropoietin/administration & dosage , Infarction, Middle Cerebral Artery/drug therapy , Neuroprotective Agents/administration & dosage , Animals , Brain/blood supply , Brain/pathology , Infarction, Middle Cerebral Artery/pathology , Infarction, Middle Cerebral Artery/physiopathology , Injections, Intraperitoneal , Male , Rats, Wistar
14.
Ross Fiziol Zh Im I M Sechenova ; 100(11): 1268-79, 2014 Nov.
Article in Russian | MEDLINE | ID: mdl-25665405

ABSTRACT

It has been shown that psychoneurological disorders are accompanied by different disturbances of immunity. Paper aimed to study the effects of repeated experience of aggression in daily agonistic interactions leading to the development of behavioral psychopathology on the parameters of cellular immunity in the thymus and spleen. There were no found the changes in the weight indexes, the number of cells in the thymus, spleen and blood in aggressive mice. In the spleen of aggressive mice percent of B-lymphocytes--CD19+ and CD16/32+, as well as T-lymphocytes CD4+8-, CD4-8+, and CD4+25(hi) decreased and percent of CD4-25+ increased in comparison with the controls. In the thymus percent of CD4-25+ cells are decreased without changes of other types of lymphocytes. Flow cytometric analysis revealed decreased percentage of apoptotic (A(0)) and resting (G0/G1) cells and increased percentage of proliferating cells in phase S+G2/M in the spleen of aggressive male mice in comparison with the control. The percentage of apoptotic thymocytes is increased and the percentage of thymocytes in S+G2/M phase is decreased under the repeated experience of aggression. Data suggest the possible development of an autoimmune procceses in male mice under the influence of repeated experience of aggression.


Subject(s)
Aggression/psychology , B-Lymphocyte Subsets/immunology , Spleen/immunology , Stress, Psychological/immunology , T-Lymphocyte Subsets/immunology , Thymus Gland/immunology , Animals , Antigens, CD/genetics , Antigens, CD/immunology , Autoimmunity , B-Lymphocyte Subsets/pathology , Cell Proliferation , G1 Phase/genetics , G1 Phase/immunology , G2 Phase Cell Cycle Checkpoints/genetics , G2 Phase Cell Cycle Checkpoints/immunology , Gene Expression , Immunity, Innate , Immunophenotyping , Lymphocyte Count , Male , Mice , Mice, Inbred C57BL , Spleen/pathology , Stress, Psychological/pathology , T-Lymphocyte Subsets/pathology , Thymus Gland/pathology
15.
Bull Exp Biol Med ; 156(1): 73-7, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24319734

ABSTRACT

We studied the effects of IL-2 administration at different times of day to female B6D2F1 mice with experimental immunopathology, graft-versus-host chronic reaction induced by the administration of lymphoid cells from parental female DBA/2 mice. Recombinant murine IL-2 was injected subcutaneously at 10.00 (group 1) or 16.00 (group 2) after the first cell transfer. Evening administration of IL-2 in contrast to its morning injection prevented cell proliferation in the thymus and spleen, decreased the number of apoptotic splenocytes, significantly stimulated differentiation processes in the thymus, increased the amount of CD4 (+) 25 (high) thymocytes, and decreased the number of CD4 (+) 27 (low) splenocytes. These findings can serve as a prerequisite for the development of chronotherapeutic schemes for immunocorrection.


Subject(s)
Cell Proliferation , Graft vs Host Disease/pathology , Immunologic Factors/administration & dosage , Interleukin-2/administration & dosage , Spleen/pathology , Thymus Gland/pathology , Animals , Drug Administration Schedule , Female , Graft vs Host Disease/drug therapy , Graft vs Host Disease/immunology , Lymphocyte Transfusion , Lymphocytes/drug effects , Lymphocytes/immunology , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Organ Size/drug effects , Spleen/drug effects , Thymus Gland/drug effects
16.
Bull Exp Biol Med ; 156(1): 86-90, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24319737

ABSTRACT

We analyzed activities of lysosomal cystein cathepsins B and L in mouse LS lymphosarcoma and its drug-resistant RLS 40 strain and their correlations with the dynamics of the percentage of cells with fragmented DNA and CD14 (+) phagocytes over 3 days after cyclophosphamide injection. LS regression and inhibition of RLS 40 growth after cyclophosphamide injection were paralleled by an increase in cathepsins B and L activities in tumor tissues. The antitumor effect of cyclophosphamide associated with apoptosis intensity and protease activities were significantly higher in LS. Positive correlations between activities of cathepsins B and L and the LS tissue content of cells with fragmented DNA and CD14 (+) phagocytes and negative correlations thereof with tumor weight were detected. It seems that the increase in cathepsins B and L activities in LS tissues was caused by cyclophosphamide induction of apoptosis and depended on the level of tumor cell infiltration with mononuclear phagocytes.


Subject(s)
Antineoplastic Agents, Alkylating/pharmacology , Apoptosis/drug effects , Cathepsin B/metabolism , Cathepsin L/metabolism , Cyclophosphamide/pharmacology , Lymphoma, Non-Hodgkin/enzymology , Phagocytes/immunology , Animals , DNA Fragmentation , Enzyme Activation , Lymphoma, Non-Hodgkin/drug therapy , Lymphoma, Non-Hodgkin/immunology , Lymphoma, Non-Hodgkin/pathology , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Neoplasm Transplantation , Phagocytes/drug effects
17.
Bull Exp Biol Med ; 156(1): 94-7, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24319739

ABSTRACT

The levels of CD19 (+) and CD20(+) microvesicles were estimated in the blood of patients with B-cell chronic lymphocytic leukemia and indolent non-Hodgkin lymphoma by flow cytometry method. It was found that the number of B cell microvesicles is several times higher in patients than in volunteers. The level of CD20 (+) microvesicles directly correlated with the number of CD20(+) lymphocytes in patients with chronic lymphoproliferative diseases. Extramedullary tumors cells can be a source of CD19 (+) microvesicles.


Subject(s)
Cell-Derived Microparticles/metabolism , Leukemia, Lymphocytic, Chronic, B-Cell/blood , Lymphoma, Non-Hodgkin/blood , Adult , Aged , Antigens, CD19/metabolism , Antigens, CD20/metabolism , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , Case-Control Studies , Female , Humans , Immunophenotyping , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Lymphocyte Count , Lymphoma, Non-Hodgkin/immunology , Male , Middle Aged
18.
Bull Exp Biol Med ; 156(2): 236-8, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24319757

ABSTRACT

Changes in the cellular and humoral immunity parameters were revealed in animals receiving thyroxin and mercazolyl per os for 2 weeks. Specific features of effects of the hormone and its inhibitor on the parameters of immune system were found.


Subject(s)
Immunity, Cellular/immunology , Immunity, Humoral/immunology , Methimazole/pharmacology , T-Lymphocyte Subsets/immunology , Thyroxine/pharmacology , Animals , Lymphocyte Count , Lymphoid Tissue/drug effects , Male , Mice , Mice, Inbred CBA , Spleen/cytology , Spleen/immunology , Thymus Gland/cytology , Thymus Gland/immunology , Thyroxine/blood , Triiodothyronine/blood
19.
Bull Exp Biol Med ; 155(6): 724-8, 2013 Oct.
Article in English | MEDLINE | ID: mdl-24288751

ABSTRACT

We studied the effect of bezafibrate on hepatic PPARα activity and immune parameters in hypertensive ISIAH rats in comparison with normotensive WAG rats under conditions of LPS treatment. Bezafibrate increased activity of PPARα in WAG rats, but not in ISIAH rats. As differentiated from WAG rats, bezafibrate produced a potent effect on the content of T cell subpopulations in the thymus and spleen of ISIAH rats. Administration of LPS after injection of bezafibrate caused death of 50% ISIAH animals (but not WAG rats), which was associated with low level of HDL cholesterol and increased triglyceride content. Our results suggest that the hypolipidemic treatment (e.g., bezafibrate) can increase the severity of complications in patients with infectious and inflammatory diseases in association with low level of HDL.


Subject(s)
Bezafibrate/toxicity , Cholesterol, HDL/blood , Hypertension/immunology , Hypolipidemic Agents/toxicity , Immunosuppressive Agents/toxicity , PPAR alpha/metabolism , Adaptive Immunity/drug effects , Animals , Hypertension/blood , Lipopolysaccharides/pharmacology , Liver/drug effects , Liver/immunology , Liver/metabolism , Lymphocyte Count , Rats , Rats, Wistar , Spleen/drug effects , Spleen/immunology , Spleen/pathology , Thymus Gland/drug effects , Thymus Gland/immunology , Thymus Gland/pathology
20.
Klin Lab Diagn ; (3): 41-4, 2013 Mar.
Article in Russian | MEDLINE | ID: mdl-23808026

ABSTRACT

The external and internal control samples are used in case of polymerase chain reaction application to ensure validity of results. The external control samples are used to establish operability of reagents included into diagnostic kit. The internal control samples make it possible to check not only all the stages of reaction but also the process of amplification in each individual test tube with reaction mixture.


Subject(s)
DNA/isolation & purification , Reference Standards , Reverse Transcriptase Polymerase Chain Reaction/methods , DNA/chemistry , Humans , Reverse Transcriptase Polymerase Chain Reaction/standards
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