ABSTRACT
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
Subject(s)
Caenorhabditis elegans Proteins/genetics , Glycine max/genetics , Proto-Oncogene Proteins c-bcl-2/genetics , Root Nodules, Plant/genetics , Salt Tolerance/genetics , Apoptosis , Caenorhabditis elegans Proteins/biosynthesis , Malondialdehyde/metabolism , Oxidative Stress , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Reactive Oxygen Species/metabolism , Root Nodules, Plant/growth & development , Glycine max/growth & developmentABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
Subject(s)
Aminobutyrates/pharmacology , Herbicides/pharmacology , Plants, Genetically Modified , Triticum/genetics , Blotting, Southern , DNA, Plant , Drug Resistance , Glucuronidase/metabolism , Models, Genetic , Plasmids/metabolism , Polymerase Chain Reaction , Time FactorsABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.
Subject(s)
Aminobutyrates/pharmacology , Herbicides , Plants, Genetically Modified , Triticum , Blotting, Southern , DNA, Plant , Glucuronidase , Models, Genetic , Plasmids/metabolism , Polymerase Chain Reaction , Drug Resistance , Time FactorsABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.(AU)
Subject(s)
Aminobutyrates/pharmacology , Herbicides/pharmacology , Plants, Genetically Modified , Triticum/genetics , Blotting, Southern , DNA, Plant , Drug Resistance , Glucuronidase/metabolism , Models, Genetic , Plasmids/metabolism , Polymerase Chain Reaction , Time FactorsABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which [quot ]model[quot ] cultivar was used.
