ABSTRACT
Toxoplasma gondii is a ubiquitous parasitic protozoan causing congenital infection and severe encephalitis in the course of the acquired immunodeficiency syndrome. Glycosyl-phosphatidylinositols of T. gondii have been shown to be identical with the low molecular weight antigen which elicits an early immunoglobulin M immune response in humans. A detailed study of the structures of these glycolipid antigens was performed. Radiolabelled glycolipids were extensively analysed by chemical and exoglycosidase treatments in combination with high pH anion-exchange chromatography, gel-filtration and lectin affinity chromatography. In addition, carbohydrate fragments prepared and purified from bulk preparations of unlabelled glycolipids by high performance liquid chromatography were subjected to two-dimensional 1H nuclear magnetic resonance spectroscopy, fast-atom bombardment-mass spectrometry, and methylation linkage analysis in order to elucidate the structure of T. gondii GPIs. The following structures were identified: (ethanolamine-PO4)-Man alpha 1-2Man alpha 1-6(GalNAc beta 1-4)Man alpha 1-4GlcN alpha-inositol-PO4-lipid and the novel structure (ethanolamine-PO4)-Man alpha 1-2Man alpha 1-6(Glc alpha 1-4GalNAc beta 1-4)Man alpha 1-4 GlcN alpha-inositol-PO4-lipid both with and without terminal ethanolamine phosphate. Evidence is provided, that only T. gondii GPIs bearing the unique glucose-N-acetylgalactosamine side branch are immunogenic in humans and that this structure is widely distributed among T. gondii isolates. Monoclonal antibodies have been characterized to recognize structures with different degrees of side-chain modification. We suggest that these reagents in combination with recently devised techniques for insertional mutagenesis in T. gondii should greatly facilitate the cloning of genes essential for GPI side-chain modification.
Subject(s)
Antigens, Protozoan/chemistry , Glycosylphosphatidylinositols/immunology , Polysaccharides/chemistry , Toxoplasma/immunology , Acetylgalactosamine/analogs & derivatives , Acetylgalactosamine/immunology , Animals , Antibodies, Monoclonal/immunology , Antigens, Protozoan/immunology , Blotting, Western , Carbohydrate Conformation , Carbohydrate Sequence , Chromatography, Affinity , Chromatography, High Pressure Liquid , Epitopes/immunology , Ethanolamine , Ethanolamines/analysis , Glucosides/chemistry , Glucosides/immunology , Glycosylphosphatidylinositols/chemistry , Humans , Magnetic Resonance Spectroscopy , Methylation , Molecular Sequence Data , Molecular Structure , Monosaccharides/analysis , Oligosaccharides/chemistry , Oligosaccharides/immunology , Polysaccharides/immunology , Polysaccharides/isolation & purification , Spectrometry, Mass, Fast Atom Bombardment , Toxoplasma/chemistry , Toxoplasmosis/immunologyABSTRACT
We are investigating the structure and biosynthesis of glycosyl-phosphatidylinositols (GPI) in the protozoa Toxoplasma gondii, Plasmodium falciparum, Plasmodium yoelii and Paramecium primaurelia. This comparison of structural and biosynthesis data should lead us to common and individual features of the GPI-biosynthesis and transport in different organisms.
Subject(s)
Eukaryota/metabolism , Glycosylphosphatidylinositols/metabolism , Animals , Glycosylphosphatidylinositols/biosynthesis , Glycosylphosphatidylinositols/chemistry , Histocytochemistry , Molecular Structure , Paramecium/metabolism , Plasmodium falciparum/metabolism , Plasmodium yoelii/metabolism , Toxoplasma/metabolismABSTRACT
The hexapeptide dimer (H-Hcy-Glu-His-Phe-D-Lys-Phe-OH)2 was synthesized using solution methods and characterized. Its conversion into H-Met(O2)-Glu-His-Phe-D-Lys-Phe-OH, Org 2766, was studied on a small scale in as short a time as possible; reduction of the disulfide bond using Na/NH3, reaction with CH3I, oxidation with H2O2 and catalyst and purification by HPLC were carried out starting with 2 mg of the dimer in a total preparation time of approximately 22 min, starting with the addition of CH3I. The preparation of the 11C-labelled analogue was carried out by methylation with 11CH3I. Restrictions imposed by working with carbon-11 will be discussed.
Subject(s)
Adrenocorticotropic Hormone/analogs & derivatives , Anti-Anxiety Agents/chemical synthesis , Peptide Fragments/chemical synthesis , Adrenocorticotropic Hormone/chemical synthesis , Amino Acid Sequence , Molecular Sequence Data , Radioactive TracersABSTRACT
Reversed-phase high-performance liquid chromatography is a valuable analytical technique to support the synthesis, isolation and purification of peptides, as is illustrated by some critical separations. In addition to this technique, capillary isotachophoresis can give useful information on the purity determination of peptides and on the presence of ionic compounds of a non-peptidic nature. With regard to the latter aspect, isotachophoresis proved to be a suitable technique as a check on the effective removal of salts after preparative high-performance liquid chromatography.
Subject(s)
Peptides/analysis , Adrenocorticotropic Hormone/analogs & derivatives , Adrenocorticotropic Hormone/analysis , Animals , Cattle , Cholecystokinin/analysis , Chromatography, High Pressure Liquid , Electrophoresis/methods , Humans , Insulin/analysis , Peptide Fragments/analysis , Spectrophotometry, Ultraviolet , Swine , Vasopressins/analysis , beta-Endorphin/analysisABSTRACT
The application of reversed-phase high performance liquid chromatography in peptide chemistry is illustrated. Its use is described for separation of closely related endorphins, as an optical purity criterion, for rapid determination of the species of origin of insulin preparations, and finally for analysis of small, basic (hydrophilic) peptides.
