Deployment and Fact Analysis of the In2Care® Mosquito Trap, A Novel Tool for Controlling Invasive Aedes Species.

During April-October 2019, the West Valley Mosquito and Vector Control District (Ontario, CA) deployed large numbers of In2Care® mosquito traps in a preliminary study to evaluate the trap's potential effectiveness at controlling invasive Aedes aegypti (L.) and Ae. albopictus (Skuse) in 6 cities of San Bernardino County, CA. The trap was used to attract ovipositing females, expose them to the juvenile hormone mimic pyriproxyfen and the entomopathogenic fungus Beauveria bassiana, and autodisseminate pyriproxyfen to other water sources prior to their death from fungal infection. The trap attracted Ae. aegypti and Culex quinquefasciatus, with the latter species predominating at much higher larval densities in the trap reservoirs. Field-collected larvae and pupae from the trap reservoirs showed complete adult emergence inhibition. Furthermore, the trap reservoirs retained high levels of residual larvicidal, pupicidal, and emergence inhibition activity after they were retrieved from the field, as indicated by laboratory bioassays against laboratory colony of Cx. quinquefasciatus. Results of this study support more detailed quantitative local evaluations on trap efficacy to measure the impact of the In2Care mosquito trap on wild invasive Aedes and Culex populations in future mosquito control efforts.

Laboratory and Field Evaluations of Mosquiron® 0.12CRD, a New Formulation of Novaluron, Against Culex Mosquitoes.

Laboratory and field evaluations were conducted to assess the activity and efficacy of Mosquiron® 0.12CRD, a new formulation containing 0.12% novaluron, against immature Culex mosquitoes. In laboratory bioassays, this formulation was highly active against Culex quinquefasciatus as indicated by low inhibition of emergence (IE) values (IE50 and IE90). When Mosquiron 0.12CRD was applied at 1 briquet per underground stormwater vault, counts of late instars showed a significant reduction on day 28 posttreatment. When the late instars and pupae collected from Mosquiron-treated water were brought back to the laboratory for posttreatment observation, emergence inhibition was >90% on day 7. When the late instars from a laboratory colony of Cx. quinquefasciatus were exposed to the Mosquiron-treated water, 99% and 95% emergence inhibition was noted on day 7 and day 14, respectively. When Mosquiron 0.12CRD was applied at 11 briquets per vault, significant reductions of larval populations were encountered on days 7 and 35 posttreatment for early instars, and on days 14, 21, and 35 posttreatment for late instars. Laboratory observation of late instars and pupae sampled from the treated vault water showed nearly complete emergence inhibition from day 7 to day 28 posttreatment. A similar trend was observed in laboratory-reared late instars of Cx. quinquefasciatus when exposed to the treated water. Preliminary evaluations indicated that Mosquiron 0.12CRD is a useful new tool to control Culex mosquitoes breeding in persistent sources.