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2.
Brasília; Sociedade Brasileira de Medicina Veterinária;Academia Brasileira de Medicina Veterinária; 2010. 192 p. ilus, tab, graf.
Monography in Portuguese | LILACS, Coleciona SUS, Sec. Munic. Saúde SP, COVISA-Acervo | ID: biblio-1556436
5.
Rio de Janeiro; Grafica & Editora OCB; 1992. 352 p. ilus.
Monography in English | LILACS | ID: lil-370258
6.
Mem. Inst. Oswaldo Cruz ; 85(4): 459-67, Oct.-Dec. 1990. tab, ilus
Article in English | LILACS | ID: lil-127783

ABSTRACT

Foureen marmosets (Callithrix penicillata) were inoculated intradermally with promastigotes and/or amastigotes of Leishmania (Viannia) brazilensis (L. (V) b.) strains MHOM/BR/83/LTB-300MHOM/BR/85/LTB-12 MHOM/BR/81/LTB-179 and MHOM/BR/82/LTB-250. The evolution of subsequent lesions was studied for 15 to 75 weeks post-inoculation (PI). All but of the L. (V) b. injected marmosets developed a cutaneous lesion at the point of inoculation after 3 to 9 weeks, characterized by the appearance of subcutaneous nodules containing parasites. parasites were isolated by culture (Difco Blood Agar) from all 11 positive animals. The maximum size of the lesions was variable and ranged between 37 mm² to 107 mm². Ulceration of primary nodules became evident after 3 to 12 weeks in all infected marmosets, but was faster and larger in 5 of the 11 animals. The active lesions persisted in 9 out of 11 Callithrix until the en of the observation period, which varied from 15-75 weeks. In 3 animals spontaneous healing of their lesions (13 to 25 weeks, PI) was observed buth with cryptic parasitism. In another 2 infected animals there was regression followed by reactivation of the cutaneous lesions. The appearance of smaller satellite lesions adjacent to primary ones, as well as metastatic lesions to the ear lobes, were documented in 2 animals. Promastigotes of L. (Leishmania) amazonensis (L.(L)a.) MHOM/BR/77/LTB-16 were inoculated in 1 marmoset. This animal remained chronically infected for 6 months and the lesions developed in a similar manner to L.(V)b. infected marmosets. No significant differences in clinical and parasitological behaviour were observed between promastigote or amastigote derived infections of the 2 species. Both produced chronic, long lasting lesions which eventually healed. The same was true for parameters of size and ulceration. Skin tests converted to parasite in 11 of 15 inected masmosets and in 1-0 of 12 parasites positive animnals. Moderate levels of circulating antibodies we also observed by IFAT/IgG assays. In spite of the failure to rep[roduce the mucosal form of the disease, an important aspect of the Callithrix model in experimental cutaneous leishmaniasis lies in the reproduction of 2 clinical events that are common in humans, namely, the chronic ulceration and spontaneous healing of the lesions


Subject(s)
Animals , Leishmania braziliensis/physiology , Leishmaniasis, Cutaneous/parasitology , Antibodies, Protozoan/analysis , Callithrix , Disease Models, Animal , Leishmania braziliensis/immunology , Leishmaniasis, Cutaneous/immunology
7.
Ciênc. cult. (Säo Paulo) ; 37(3): 430-9, mar. 1985. mapas, tab
Article in Portuguese | LILACS | ID: lil-30638

Subject(s)
Veterinary Medicine
9.
Article in Spanish | PAHO | ID: pah-33950

ABSTRACT

The reactivation of foci of human bubonic plague in the endemic areas of Brazil during the period 1964-1966 led to checking the usefulness of the hemagglutination microtechnique test in diagnosing the disease in rural areas of the States of Bahia and Minas Gerais. Both the clinical and the bacteriological diagnoses were made in 1965 and 1966, and the blood samples used for the test came from patients who had recovered from the disease


Three differente groups were tested. The first consisted of 11 persons, among whom those who had a serum titer of 1:24 or over were considered positive; the second and third groups consisted of 30 and 8 persons, respectively, among whom agglutinations observed at 1:4 and 1:8 dilutions were considered suspect, and agglutinations observed at dilutions of 1:16 or over were recorded as positive. The serological tests in all three grups were made with tanned red blood cells of sheep (Group A), formolized and tanned (Groups B and C), and sensitized with fraction 1 (F1) or Pasteurella pestis. To dilute the sera in all cases, a saline solution plus normal 5 per cent rabbit serum, inactive and adsorbed in red blood cell of sheep, were used


Survey results indicated that retrospective diagnoses of human bubonic plague could be obtained through the hemagglutination test, using the microtechnique method. The test made it possible to confirm the clinical and ...(AU)


Subject(s)
Plague/diagnosis , Hemagglutination Tests , Rural Population , Yersinia pestis , Clinical Laboratory Techniques/methods , Brazil
10.
Article | PAHO-IRIS | ID: phr-15280

ABSTRACT

The reactivation of foci of human bubonic plague in the endemic areas of Brazil during the period 1964-1966 led to checking the usefulness of the hemagglutination microtechnique test in diagnosing the disease in rural areas of the States of Bahia and Minas Gerais. Both the clinical and the bacteriological diagnoses were made in 1965 and 1966, and the blood samples used for the test came from patients who had recovered from the disease


Three differente groups were tested. The first consisted of 11 persons, among whom those who had a serum titer of 1:24 or over were considered positive; the second and third groups consisted of 30 and 8 persons, respectively, among whom agglutinations observed at 1:4 and 1:8 dilutions were considered suspect, and agglutinations observed at dilutions of 1:16 or over were recorded as positive. The serological tests in all three grups were made with tanned red blood cells of sheep (Group A), formolized and tanned (Groups B and C), and sensitized with fraction 1 (F1) or Pasteurella pestis. To dilute the sera in all cases, a saline solution plus normal 5 per cent rabbit serum, inactive and adsorbed in red blood cell of sheep, were used


Survey results indicated that retrospective diagnoses of human bubonic plague could be obtained through the hemagglutination test, using the microtechnique method. The test made it possible to confirm the clinical and ...(AU)


Subject(s)
Plague , Yersinia pestis , Hemagglutination Tests , Rural Population , Brazil
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