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1.
J Mol Biol ; 208(3): 429-43, 1989 Aug 05.
Article in English | MEDLINE | ID: mdl-2477552

ABSTRACT

Site-directed nuclease digestion and nonsense mutations of the Escherichia coli metG gene were used to produce a series of C-terminal truncated methionyl-tRNA synthetases. Genetic complementation studies and characterization of the truncated enzymes establish that the methionyl-tRNA synthetase polypeptide (676 residues) can be reduced to 547 residues without significant effect on either the activity or the stability of the enzyme. The truncated enzyme (M547) appears to be similar to a previously described fully active monomeric from of 64,000 Mr derived from the native homodimeric methionyl-tRNA synthetase (2 x 76,000 Mr) by limited trypsinolysis in vitro. According to the crystallographic three-dimensional structure at 2.5 A resolution of this trypsin-modified enzyme, the polypeptide backbone folds into two domains. The former, the N-domain, contain a crevice that is believed to bind ATP. The latter, the C-domain, has a 28 C-residue extension (520 to 547), which folds back, toward the N-domain and forms an arm linking the two domains. This study shows that upon progressive shortening of this C-terminal extension, the enzyme thermostability decreases. This observation, combined with the study of several point mutations, allows us to propose that the link made by the C-terminal arm of M547 between its N and C-terminal domains is essential to sustain an active enzyme conformation. Moreover, directing point mutations in the 528-533 region, which overhangs the putative ATP-binding site, demonstrates that this part of the C-terminal arm participates also in the specific complexation of methionyl-tRNA synthetase with its cognate tRNAs.


Subject(s)
Amino Acyl-tRNA Synthetases/metabolism , Escherichia coli/genetics , Methionine-tRNA Ligase/metabolism , Peptides/metabolism , RNA, Bacterial/genetics , RNA, Transfer/genetics , Acylation , Binding Sites , Genes, Bacterial , Genetic Complementation Test , Hot Temperature , Models, Molecular , Structure-Activity Relationship , Trypsin
2.
Biochimie ; 70(6): 773-82, 1988 Jun.
Article in English | MEDLINE | ID: mdl-3139093

ABSTRACT

The construction of a family of plasmids carrying derivatives of metG, the gene for E. coli methionyl-tRNA synthetase, is described. These plasmids allow expression of native or truncated forms of the enzyme and easy purification of the products. To facilitate the characterization of modified enzymes with very low catalytic activity, a specialized vector was constructed, in which metG was fused in frame with lacZ, the gene for beta-galactosidase. This plasmid expresses a methionyl-tRNA synthetase-beta-galactosidase chimeric protein, which is shown to carry the activities of both enzymes. This hybrid can be purified in a single step of affinity chromatography for beta-galactosidase. The methionyl-tRNA synthetase moiety can be regenerated by mild proteolysis, thus providing a simple method for purifying and studying mutated proteins.


Subject(s)
Amino Acyl-tRNA Synthetases/genetics , Chimera , Galactosidases/metabolism , Methionine-tRNA Ligase/genetics , Protein Engineering/methods , beta-Galactosidase/metabolism , Escherichia coli/genetics , Genetic Vectors , Mutation , Peptide Hydrolases/metabolism , Plasmids
3.
J Bacteriol ; 164(1): 63-9, 1985 Oct.
Article in English | MEDLINE | ID: mdl-2995325

ABSTRACT

A clone overproducing diadenosine tetraphosphatase (diadenosine 5', 5'''-P1, P4-tetraphosphate pyrophosphohydrolase) activity was isolated from an Escherichia coli cosmid library. Localization of the DNA region responsible for stimulation of this activity was achieved by deletion mapping and subcloning in various vectors. Maxicell experiments and immunological assays demonstrated that a 3.5-kilobase-pair DNA fragment carried the structural gene apaH encoding the E. coli diadenosine tetraphosphatase. The DNA coding strand was determined by cloning this fragment in both orientations in pUC plasmids. It was also shown that the overproduction of diadenosine tetraphosphatase decreased the dinucleoside tetraphosphate concentration in E. coli by a factor of 10.


Subject(s)
Acid Anhydride Hydrolases , Cloning, Molecular , Escherichia coli/genetics , Genes, Bacterial , Phosphoric Diester Hydrolases/genetics , Cosmids , DNA, Bacterial/analysis , Dinucleoside Phosphates , Genes , Oligonucleotides/analysis , Phosphoric Diester Hydrolases/biosynthesis , Transcription, Genetic
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